scholarly journals Effect of Duyun Compound Green Tea on Gut Microbiota Diversity in High-Fat-Diet-Induced Mice Revealed by Illumina High-Throughput Sequencing

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Caibi Zhou ◽  
Xiaolu Zhou ◽  
Zhirui Wen ◽  
Zaibo Yang ◽  
Ren Mu ◽  
...  
Keyword(s):  
Treatment Group ◽  
Green Tea ◽  
Intestinal Microbiota ◽  
High Throughput ◽  
High Fat Diet ◽  
Intestinal Tract ◽  
High Throughput Sequencing ◽  
Control Group ◽  
High Fat ◽  
Beneficial Bacteria

Intake of a high-fat diet (HFD) is closely related to disorders of the intestinal microbiota, which plays a key role in the pathogenesis of obesity. Duyun compound green tea, an ancient Chinese drink, is widely consumed to reduce weight, although the mechanism is not clear. In this study, 50 mice were randomly divided into 5 groups: normal control group (CK), HFD model control group (NK), positive control group with medicine (YK), low-dose compound tea group (DL), and high-dose compound tea group (DH). After 4 weeks of intervention, the feces of mice were taken under sterile conditions and evaluated using Illumina high-throughput sequencing technology. The results showed that the diversity of intestinal microbiota was the highest in the CK group, the lowest in the NK group, and relatively increased in the compound tea treatment group. Second, there were differences in intestinal microbiota in each group, among which the beneficial bacteria in the intestinal tract of the CK group were higher than those in the other groups, while the beneficial bacteria in each compound tea treatment group were more abundant than those in the NK group, in which harmful bacteria in the intestinal tract were found to be the highest. These results suggest that compounds in tea may be able to attenuate imbalances of intestinal microbiota induced by poor diet, acting as a therapeutic agent in obesity or other diseases associated with gut dysbiosis.

scholarly journals Histologi Lumen Dan Endotelium Arteri Dorsal Penis Tikus Wistar (Rattus Novergicus) Yang Diinduksi Pakan Tinggi Lemak

2020 ◽  
Vol 7 (1) ◽  
pp. 73
Author(s):  
I Wayan Rosiana ◽  
I Gede Widhiantara
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Lumen Diameter ◽  
Control Group ◽  
High Fat ◽  
Arterial Lumen ◽  
Male Wistar Rats ◽  
The Difference

This study aims to look at the histopathological picture of the dorsal arteries of the penis of the hiperlipidemic wistar rats (Rattus novergicus) induction by high-fat diet that seen in terms of lumen diameter and thickness of the arterial endotelium wall. Hyperlipidemia is a risk factor for ateriosclerosis in the penile arteries causing erectile dysfunction in men. This study is an experimental study with a randomized posttest only control goup design. The sample are  10 individuals adult male wistar rats aged 3-4 months with a range of body weight 150-200 grams. Before treatment, adaptation was carried out for 7 days. After that the sample rats in the treatment group were made hyperlidemic by feeding lard for 50 days. Then surgery is performed for histopathological preparations at the posttest. To determine the differences in endotelium thickness and arterial lumen diameter in the two groups, an independent t-test was used. Thick diameter data of the endotelium and dorsal arteries of the penis of the wistar rat between the lower treatment group and the control group. The difference that occurred was statistically significant (p <0.05). So it can be concluded that the provision of high-fat diet (hyperlipidemia) decreases the lumen diameter and endotelium thickness of dorsal arteries penis. Keywords: Dorsal arteries, high-fat diet, Wistar rats

scholarly journals Inulin from Jerusalem artichoke tubers alleviates hyperglycaemia in high-fat-diet-induced diabetes mice through the intestinal microflora improvement

2019 ◽  
Vol 123 (3) ◽  
pp. 308-318 ◽  
Author(s):  
Tianyun Shao ◽  
Qiuhong Yu ◽  
Tingshuo Zhu ◽  
Anhong Liu ◽  
Xiumei Gao ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Intestinal Microbiota ◽  
High Fat Diet ◽  
High Throughput Sequencing ◽  
Fasting Blood Glucose ◽  
Jerusalem Artichoke ◽  
Differentially Expressed ◽  
High Fat ◽  
Blood Concentrations ◽  
Linear Discriminant

AbstractThe rate of hyperglycaemia in people around the world is increasing at an alarming rate at present, and innovative methods of alleviating hyperglycaemia are needed. The effects of Jerusalem artichoke inulin on hyperglycaemia, liver-related genes and the intestinal microbiota in mice fed a high-fat diet (HFD) and treated with streptozotocin (STZ) to induce hyperglycaemia were investigated. Inulin-treated hyperglycaemic mice had decreased average daily food consumption, body weight, average daily water consumption and relative liver weight and blood concentrations of TAG, total cholesterol, HDL-cholesterol and fasting blood glucose. Liver-related gene expressions in hyperglycaemic (HFD-fed and STZ-treated) compared with control mice showed eighty-four differentially expressed genes (forty-nine up-regulated and thirty-five down-regulated). In contrast, hyperglycaemic mice treated with inulin had twenty-two differentially expressed genes compared with control ones. Using Illumina high-throughput sequencing technology, the rarefaction and the rank abundance curves as well as the α diversity indices showed the treatment-induced differences in bacterial diversity in intestine. The linear discriminant analysis of effect size showed that the inulin treatment improved intestinal microbiota; in particular, it significantly increased the number of Bacteroides in the intestine of mice. In conclusion, inulin is potentially an effective functional food for the prevention and/or treatment of hyperglycaemia.

scholarly journals The effect of aerobic exercise on relative leukocyte telomere length in male Sprague-Dawley rats given a high fat-diet

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1143
Author(s):  
Dewi Irawati Soeria Santoso ◽  
Nurul Paramita ◽  
Ani Retno Prijanti ◽  
Thressia Hendrawan ◽  
Swandito Wicaksono
Keyword(s):  
Treatment Group ◽  
Aerobic Exercise ◽  
Telomere Length ◽  
High Fat Diet ◽  
Control Group ◽  
Sprague Dawley ◽  
High Fat ◽  
White Rats ◽  
Relative Telomere Length ◽  
Length Changes

Background: There is an increasing number of studies showing that physical activity and aerobic exercise have a positive effect on telomere length. Some studies also show that dynamics of telomere length is influenced by various environmental factors such as lifestyle and diet. However, the association between exercise and diet with telomere length is still questionable. The aim of this study was to examine the effects of aerobic physical exercise on relative telomere length changes in high fat-diet condition in rat animal models. Methods: This study was an in vivo experimental study using twelve Sprague-Dawley male white rats (12-month-old). Subjects were evenly and randomly divided into two groups (n=6): (1) high fat-diet fed control group; (2) high fat-diet fed and aerobic exercise treatment group. Aerobic exercise was conducted using animal treadmill with intensity of 20 m/min, 5 days/week. At weeks 4 and 8, relative telomere length was compared with week 0 control group, using q-RT-PCR. Results: Lengthening of relative telomere length was observed in both control and treatment groups at weeks 4 and 8, when compared to week 0 control group. The lengthening in the control group was much greater than the treatment group. Conclusions: Excessive increase of relative telomere length was seen in high fat-diet conditions. Aerobic exercise for 8 weeks suppresses excessive increase of relative telomere length in high fat-diet conditions.

scholarly journals Effect of succinic acid on the organism of mice and their intestinal microbiota against the background of excessive fat consumption

2020 ◽  
Vol 11 (2) ◽  
pp. 153-161
Author(s):  
M. A. Lieshchova ◽  
M. V. Bilan ◽  
A. A. Bohomaz ◽  
N. M. Tishkina ◽  
V. V. Brygadyrenko
Keyword(s):  
Succinic Acid ◽  
Intestinal Microbiota ◽  
High Fat Diet ◽  
Hepatic Function ◽  
Functional Condition ◽  
Laboratory Animals ◽  
Relative Mass ◽  
Control Group ◽  
Quantitative Composition ◽  
High Fat

Succinic acid and its salts (succinates) positively affect the oxygenation of the internal environment, stabilize the structure and functional activity of mitochondria, and normalize the ion metabolism in the cell. Separate clinical studies and experimental surveys confirmed that having low toxicity succinic acid has well-manifested antioxidant, immunostimulating, adaptogenic properties. In this study, we determined the influence of succinic acid on the organism of laboratory animals against the backround of high-fat diet: the changes in body weight, indices of the mass of the internal organs, blood parameters and the changes in the intestinal microbiota were determined. For the experiment, we formed three experimental and three control groups of male white mice. The animals of the control group received 0.5% solution of succinic acid instead of water. In the experiment, we determined that succinic acid has no effect on the intensity of growth of weight of young mice against the background of excessive fat in their diet. Excessive consumption of fat by male mice leads to mainly disorders in the functioning of the liver, excretory and the immune systems. High-fat diet of mice is accompanied by impaired hepatic function, manifested in sharp hypoproteinemia due to globulins, increase in the activity of hepatic enzymes against the background of reduced activity of alkaline phosphatase, increase in the level of bilirubin, and decrease in glucose. Excess of fat in the diet leads to malfunctioning of the excretory system, manifested in the reduced index of kidneys’ weight, high content of creatinine and reduced level of urea in the blood. Addition of succinic acid has a positive effect on the functional condition of the liver and the kidneys, especially noticeable during long-term intake. High-fat diet causes disorders in the functioning of the organs of blood circulation and immune protection, accompanied by decrease in the relative mass of the thymus and spleen, low content of hemoglobin and the number of erythrocytes, but has no significant effect on the content of other cellular elements in the blood. By the middle of the experiment, succinic acid had exacerbated these processes compared to the control, but by the end of the experiment, by contrast, these processes were alleviated. Addition of the succinic acid to high-fat diet contributed to the change in the quantitative composition of the main representatives of the obligatory microbiota (Bifidobacterium spp., Lactobacillus spp. and typical Escherichia coli) in the laboratory animals. Such changes in the intestinal microbiota may lead to such consequences as reproduction of the facultative microflora, and, thus, development of various diseases.

scholarly journals Citrobacter Species Increase Energy Harvest by Modulating Intestinal Microbiota in Fish: Nondominant Species Play Important Functions

mSystems ◽  
2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Mei-Ling Zhang ◽  
Miao Li ◽  
Yi Sheng ◽  
Fang Tan ◽  
Liqiao Chen ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Intestinal Microbiota ◽  
Lipid Accumulation ◽  
High Fat Diet ◽  
High Throughput Sequencing ◽  
Intestinal Bacteria ◽  
Absorption Efficiency ◽  
Energy Harvest ◽  
High Fat ◽  
Microbial Composition

ABSTRACT An efficient energy harvesting mechanism is likely critical for animals in their natural environment. Intestinal microbiota enriched by a high-fat diet aid in lipid accumulation, a strategy likely evolved for energy harvest in mammals. However, whether this strategy is conserved among vertebrate organisms remains unclear. A bacterial strain (S1), enriched on soybean oil rich medium, was isolated from the gut of Nile tilapia and demonstrated to be a member of the Citrobacter genus. Although a high-fat diet increased the number of Citrobacter spp., these bacteria were not abundant in the intestine by high-throughput sequencing. Addition of bacterium S1 to a high-fat diet modulated intestinal microbial composition and increased high-fat diet-induced lipid accumulation in mesenteric adipose tissue, accompanied by (i) increased triglyceride absorption efficiency and triglyceride reesterification and (ii) increased intestinal permeability. Collectively, our results provide evidence that specific intestinal bacteria aid the host in harvesting more energy from a high-fat diet in fish. Furthermore, the results from the present study also suggest that nondominant bacteria in the gut may play an important role in regulating host metabolism. IMPORTANCE This study shows that the ability of gut microbiota members to enhance host energy harvest from a high-fat diet is a conserved feature of host-microbe interactions in fish, as in mammals. It also underscores that gut microbiota members are able to significantly impact host biology even when at low abundance.

scholarly journals Comparative analysis of gut bacterial communities in housefly larvae fed different diets using a high-throughput sequencing approach

2019 ◽  
Vol 366 (11) ◽  
Author(s):  
Zhijing Xue ◽  
Junli Zhang ◽  
Ruiling Zhang ◽  
Zhendong Huang ◽  
Qing Wan ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
High Throughput ◽  
Bacterial Communities ◽  
High Fat Diet ◽  
High Throughput Sequencing ◽  
Animal Feed ◽  
Intestinal Bacteria ◽  
High Protein ◽  
Fish Feed ◽  
High Fat

ABSTRACT Housefly larvae are a synanthropic host for various bacteria, including pathogens and commensals and an important protein source for monogastric animal feed. Many factors, such as diets, life stages, host habitats can influence microbial community structure. In this study, the diversity of bacterial communities in the gut of housefly larvae fed on different artificial diets was comprehensively characterized using high-throughput sequencing with the aim shedding light on an optimal larval diet. The results showed that the dominant bacteria belonging to Proteobacteria, Firmicutes and Bacteroidetes phyla were related to polysaccharide degradation. The comparative analysis indicated that the dominant intestinal bacteria of larvae fed on high-protein were similar to those on high-fat diet. The same was the case in larvae fed high-starch diet and wheat bran alone. In addition, the diversity of intestinal bacteria at genus level in larvae fed high-protein and high-fat diet was higher than in larvae fed the other two diets. Further analysis indicated that the increase of potential commensals and decrease of pathogens in larvae fed on high-fat diet contributed to the increase of housefly larvae immunity. It established a foundation for further research on improvement of nutrition of housefly larvae used for poultry and fish feed.

scholarly journals Effect of Kelussia odoratissima Mozaff extract on PNPLA3 gene expression in non-alcoholic fatty liver and control rats

2021 ◽  
pp. 335-345
Keyword(s):  
Gene Expression ◽  
Treatment Group ◽  
Fatty Liver ◽  
High Fat Diet ◽  
Blood Lipids ◽  
Control Group ◽  
Protective Effects ◽  
High Fat ◽  
Biochemical Tests ◽  
Alcoholic Fatty Liver

Background and Aims: Non-alcoholic fatty liver disease (NAFLD) is associated with such symptoms as steatosis, fibrosis, and liver cirrhosis. Kelussia has attracted assiduous attention due to its protective effects on the liver. The PNPLA3 gene is mainly expressed in the liver and plays a major role in the degradation rate of hepatic triglycerides. Therefore, the present study aimed to assess the effect of Kelussia extract on PNPLA3 gene expression in rats with fatty liver and healthy rats. Materials and Methods: This experimental study was conducted on 24 male Wistar rats in the control group (no treatment), obese group (which received a high-fat diet), treatment group 1 (which received a high-fat diet with Kelussia extract 400 mg/kg) and treatment group 2 (a high-fat diet with Kelussia extract 800 mg/kg) for six weeks. Blood samples were taken from rats and the factors of (LDL, HDL, Cholesterol, Triglyceride, and fasting sugar) were measured. After sampling the rat liver, the effect of Kelussia on PNPLA3 gene expression was investigated using the Real-time reverse transcription-polymerase chain reaction (RT-PCR) technique and analyzed in SPSS software (version 22). Results: Based on the results, Kelussia extract at a dose of 800 mg/kg resulted in a more dramatic decrease in PNPLA3 gene expression in rats with fatty liver, compared to a dose of 400 mg /kg, and this reduction was statistically significant, compared to the fatty liver group (P<0.05). The results of biochemical tests confirmed liver improvement in the rats treated with Kelussia extract at a dose of 800 mg/kg. Conclusion: It can be said that Kelussia had a beneficial effect on the reduction of blood lipids; moreover, it reduces the accumulation of triglycerides in the liver and improves the tissue structure of the liver by reducing the expression of PNPLA3 gene; therefore, with more studies, it can be considered a supplement to reduce blood lipids.

scholarly journals Effect of Green Tea Ingestion on Postprandial Triglyceride Levels in Young Women

2017 ◽  
Vol 1 (1) ◽  
pp. 23
Author(s):  
Trismiyanti Trismiyanti ◽  
Ida Gunawan ◽  
Tri Juli Edi Tarigan
Keyword(s):  
Treatment Group ◽  
Green Tea ◽  
Young Women ◽  
Intestinal Lumen ◽  
Group Method ◽  
Control Group ◽  
Plain Water ◽  
High Fat ◽  
Postprandial Triglyceride ◽  
Fat Meal

Introduction: High blood triglyceride (TG) level is a risk factor for cardiovascular disease. Green tea, as a beverage, may reduce postprandial blood TG level through inhibition of fat absorption in the intestinal lumen. The aim of this study was to assess the difference of postprandial blood TG level changes between treatment group (high-fat meal and green tea beverage containing 738 mg of catechins) and control group (high-fat meal and plain water containing 0 mg of catechins).Methods: The study was a randomized, single-blind, parallel-group clinical trial including 40 healthy young women. Blood was collected immediately before the meal and then at 2 and 4 h thereafter of each group. Method of TG measurement: GPO-PAP, using Beckman CX 5-CE machine and Good’s buffer reagent.Results: Postprandial blood TG level at 4 h in the treatment group was significantly lower compared to the control group (1.00 ± 0.27 vs. 1.22 ± 0.34 mmol/L, p = 0.03). The change in blood TG level from baseline to 4 h postprandial was also significantly lower in the treatment group compared to the control group (0.21 ± 0.14 vs. 0.37 ± 0.26 mmol/L, p = 0.02).Conclusion: It was concluded that green tea ingestion during high fat meal suppressed postprandial elevation of TG 4 hour after meal.

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