scholarly journals Histologi Lumen Dan Endotelium Arteri Dorsal Penis Tikus Wistar (Rattus Novergicus) Yang Diinduksi Pakan Tinggi Lemak

2020 ◽  
Vol 7 (1) ◽  
pp. 73
Author(s):  
I Wayan Rosiana ◽  
I Gede Widhiantara
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Wistar Rat ◽  
Lumen Diameter ◽  
Control Group ◽  
High Fat ◽  
Arterial Lumen ◽  
Male Wistar Rats ◽  
The Difference

This study aims to look at the histopathological picture of the dorsal arteries of the penis of the hiperlipidemic wistar rats (Rattus novergicus) induction by high-fat diet that seen in terms of lumen diameter and thickness of the arterial endotelium wall. Hyperlipidemia is a risk factor for ateriosclerosis in the penile arteries causing erectile dysfunction in men. This study is an experimental study with a randomized posttest only control goup design. The sample are  10 individuals adult male wistar rats aged 3-4 months with a range of body weight 150-200 grams. Before treatment, adaptation was carried out for 7 days. After that the sample rats in the treatment group were made hyperlidemic by feeding lard for 50 days. Then surgery is performed for histopathological preparations at the posttest. To determine the differences in endotelium thickness and arterial lumen diameter in the two groups, an independent t-test was used. Thick diameter data of the endotelium and dorsal arteries of the penis of the wistar rat between the lower treatment group and the control group. The difference that occurred was statistically significant (p <0.05). So it can be concluded that the provision of high-fat diet (hyperlipidemia) decreases the lumen diameter and endotelium thickness of dorsal arteries penis. Keywords: Dorsal arteries, high-fat diet, Wistar rats

scholarly journals Protective role of melatonin against adipose-hepatic metabolic comorbidities in experimentally induced obese rat model

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260546
Author(s):  
Mary J. Obayemi ◽  
Christopher O. Akintayo ◽  
Adesola A. Oniyide ◽  
Ayodeji Aturamu ◽  
Olabimpe C. Badejogbin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Food Intake ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Liver Lipid ◽  
Control Group ◽  
Metabolic Dysfunction ◽  
High Fat ◽  
Male Wistar Rats

Background Adipose and hepatic metabolic dysfunctions are critical comorbidities that also aggravate insulin resistance in obese individuals. Melatonin is a low-cost agent and previous studies suggest that its use may promote metabolic health. However, its effects on some comorbidities associated with obesity are unknown. Herein, we investigated the hypothesis that melatonin supplementation would attenuate adipose-hepatic metabolic dysfunction in high fat diet (HFD)-induced obesity in male Wistar rats. Materials and methods Twenty-four adult male Wistar rats (n = 6/group) were used: Control group received vehicle (normal saline), obese group received 40% high fat diet, melatonin-treated group received 4 mg/kg of melatonin, and obese plus melatonin group received 40% HFD and melatonin. The treatment lasted for 12 weeks. Results HFD caused increased food intake, body weight, insulin level, insulin resistance and plasma and liver lipid but decreased adipose lipid. In addition, HFD also increased plasma, adipose and liver malondialdehyde, IL-6, uric acid and decreased Glucose-6-phosphate dehydrogenase, glutathione, nitric oxide and circulating obestatin concentration. However, these deleterious effects except food intake were attenuated when supplemented with melatonin. Conclusion Taken together, the present results indicate that HFD exposure causes adipose-hepatic metabolic disturbance in obese animals, which are accompanied by oxidative stress and inflammation. In addition, the present results suggest that melatonin supplementation attenuates adipose-hepatic metabolic dysfunction, accompanying obesity by suppression of oxidative stress/inflammation-dependent mechanism and increasing circulating obestatin.

scholarly journals Uji efektivitas ekstrak daun sendok (Plantago major L.) terhadap waktu perdarahan pada tikus Wistar jantan (Rattus norvegicus)

e-GIGI ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Abedneju R. Kainde ◽  
Damajanty H.C. Pangemanan ◽  
Bernart S.P. Hutagalung
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Bleeding Time ◽  
Wistar Rat ◽  
Control Group ◽  
P Value ◽  
Plantago Major ◽  
Control Group Design ◽  
Group Design ◽  
Male Wistar Rats

Abstract: Herbal plant is often used to treat any kind of disease. There are many kinds of plants in Minahasa used as traditional medicine, inter alia daun sendok (Plantago major L.). Daun sendok is often used to treat bleeding. This study was aimed to obtain the effectiveness of daun sendok extract on bleeding time in male Wistar rats. This was a true experimental study with a posttest only group design. This study was conducted at the Laboratory of Pharmacology Faculty of Medicine University of Sam Ratulangi. Total samples were 14 rats divided into 2 groups: control group and treatment group. Wounds of 2 mm depth were perfomed on the rat tails of the treatment group followed by application of daun sendok extract. The results showed that the average bleeding time of the control group was 51.87 seconds meanwhile of the treatment group was 29.67 seconds. The unpaired t-test between the two groups showed a p value of 0.000. Conclusion: Daun sendok (Plantago major L.) extract could reduce the bleeding time in male Wistar rats. Keywords: bleeding time, daun sendok, Wistar rat Abstrak: Tanaman obat seringkali digunakan untuk mengobati berbagai macam jenis penyakit. Di wilayah Minahasa terdapat banyak jenis tanaman yang digunakan sebagai obat tradisional, salah satunya ialah daun sendok (Plantago major L.) yang seringkali digunakan untuk mengobati perdarahan. Penelitian ini berrtujuan untuk mengetahui efektivitas ekstrak daun sendok terhadap waktu perdarahan tikus Wistar jantan. Jenis penelitian ialah true experimental dengan post test only control group design yang dilakukan di Laboratorium Farmakologi Fakultas Kedokteran Unsrat dengan total sampel berjumlah 14 ekor tikus Wistar yang dibagi menjadi 2 kelompok yaitu: kelompok kontrol dan kelompok perlakuan menggunakan ekstrak daun sendok. Pada kelompok uji diaplikasikan ekstrak daun sendok setelah dilakukan perlukaan di bagian ekor tikus sedalam 2 mm. Hasil penelitian memperlihatkan rerata waktu perdarahan pada kelompok kontrol ialah 51,87 detik sedangkan pada kelompok perlakuan 29,67 detik. hasil uji t tidak berpasangan antara kedua kelompok mendapatkan nilai p=0,000. Simpulan: Ekstrak daun sendok (Plantago major L.) efektif dalam memperpendek waktu perdarahan pada tikus Wistar.Kata kunci: waktu perdarahan, daun sendok, tikus Wistar

Bacterial Conjugated Linoleic Acid Effect on Hepatic and Adipose Tis-sues of High-Fat diet-Induced Obese Rats

2016 ◽  
Vol 6 (4) ◽  
pp. 143-149
Author(s):  
Imene Yahla ◽  
Hasnia Ziar ◽  
Mohamed Benali ◽  
Ali Riazi
Keyword(s):  
Linoleic Acid ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Experimental Period ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Adipose Tissues ◽  
Acid Effect ◽  
Male Wistar Rats

The aim of the present study is to background the effect of conjugated lino-leic acid isomers (CLA) produced by two probiotic strains, Lactobacillus rham-nosus LBRE-LSAS (a human originated bacterium) and Bifidobacterium ani-malis subsp lactis Bb12, on both hepatic and adipose tissues of high-fat diet fed Wistar rats. Five-week-old male Wistar rats were divided into 4 groups (n=6) fed a high-fat diet for three of them (control and supplemented with 1x109 CFU per rat of LBRE-LSAS or Bb12 strain and 1.4% of free linoleic acid; designed as treated rats) and a standard diet for the fourth group. After 8 weeks of experimental period, rats were sacrificed after chloroform anesthe-sia; livers and adipose tissues of each group were excised for biochemical and histological analyses. Obtained results showed that livers of treated high-fat diet fed rats did not exhibit a hepatic steatosis like those of untreated high-fat diet fed rats (control group) did. Lipid profile (triglycerides and total cholesterol) of the liver and the adipose tissue was markedly improved in treated rat groups, especially in LBRE-LSAS strain given high-fat diet rats. Such results strongly support the occurrence of the bacterial power of Lacto-bacillus rhamnosus LBRE-LSAS and Bifidobacterium animalis subsp lactis Bb12 to modulate lipid metabolism and to avoid steatosis in diet-induced model of obesity in rat.

scholarly journals Test of the Effectiveness of Anti-Dyslipedemia Extract Ethanol Mangosteen Peel (Garcinia Mangostana L.) in Male Mice Fed a High-Fat Diet

2021 ◽  
Vol 4 (1) ◽  
pp. 1-12
Author(s):  
Zhang Yu ◽  
Florenly ◽  
Liena ◽  
Fioni
Keyword(s):  
Treatment Group ◽  
High Fat Diet ◽  
Ethanol Extract ◽  
Control Group ◽  
High Fat ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Entire Treatment

Dyslipidemia is a major risk factor for atheroscler heart disease, stroke, and is often defined as abnormalities or disruption of lipid metabolism. Garcinia mangostana L. is famous for its anti-inflammatory properties and is used in the treatment of skin infections and wounds. The main phytochemicals present in this species are anisoprenylated xanthone, many reports of biological effects, such as antioxidant, pro-apoptosis, anti-proliferative, anti-nosiseptif, anti-inflammatory, neuroprotective, hypoglycemic, and anti-obesity. This study aims to find out the effectiveness of mangosteen peel ethanol extract as an anti-dyslipidemia in male wistar rats given a high-fat diet. This type of research is experimental with a Pre-test approach and Post-test group only control design. The samples used were mangosteen peel ethanol extract and male wistar rats, with the size of the sample calculated with Federer's formula. Analyze data with the One-Way Anova Test if the data is normally distributed with advanced tests in the form of Post Hoc Tukey HSD tests to see real differences between treatments. The results of the total cholesterol study in the entire treatment group of mice showed a significant difference in P values < 0.05. Triglyceride levels in the entire treatment group also showed significant differences, this can be seen from the value of P < 0.05 (Value P = 0.029). LDL levels also showed significant differences across the treatment group, which can be seen from the P value of < 0.05. HDL levels make a significant difference in the value of P < 0.05 (Value P = 0.029). SGOT and SGPT levels in the entire group of rat treatment showed significant differences, this was seen from the value of P < 0.05. The conclusion that mangosteen peel ethanol extract significantly lowered total cholesterol, triglyceride levels, LDL levels, SGOT levels compared to the control group. Mangosteen peel ethanol extract can significantly increase HDL levels compared to the control group.

Melatonin ameliorates endocrine dysfunction and defective sperm integrity associated with high‐fat diet‐induced obesity in male Wistar rats

Andrologia ◽  
2021 ◽  
Author(s):  
Comfort Abisola Oladele ◽  
Christopher Oloruntoba Akintayo ◽  
Olabimpe Caroline Badejogbin ◽  
Adesola Adedotun Oniyide ◽  
Adams Olalekan Omoaghe ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Wistar Rats ◽  
Endocrine Dysfunction ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Male Wistar Rats

Particular Matter of Motor Vehicle Exposure and High-Fat Diet Effects on Kidney Histopathology, Creatinine, and Malondialdehyde (MDA) Levels in Wistar Rats

2021 ◽  
Vol 5 (3) ◽  
pp. 124-128
Author(s):  
Rizka Veni ◽  
Awal Prasetyo ◽  
Muflihatul Muniroh
Keyword(s):  
High Fat Diet ◽  
Wistar Rats ◽  
Motor Vehicle ◽  
Histopathological Change ◽  
Normal Diet ◽  
Control Group ◽  
High Fat ◽  
Control Group Design ◽  
Treatment Groups ◽  
Significant Difference

This study aims to analyze the effect of combination of motor vehicle particular matter exposure and high-fat diet in kidney histopathology, creatinine levels, and MDA levels in Wistar rats. This study used a posttest-only control group design. Eighteen healthy male Wistar rats were divided into three groups. The intervention groups received motor vehicle fume exposure for 100 s with normal diet (X1) or high-fat diet (X2), and the control group received no exposure (C). Data analysis was processed with a SPSS 25.0 computer program by using the one-way ANOVA test followed by post hoc LSD. The degree of kidney histopathological damage showed significant differences between the X1 and X2 groups when compared with the control group (p < 0.05). The results of the creatinine level examination found a significant difference between the X2 and C groups (p < 0.05) and the treatment groups X1 and X2 (p < 0.05). The results of kidney MDA level examination showed a significant difference between the treatment groups (X1 and X2) and the control group (p < 0.05). The combination of particular matter of motor vehicle fumes exposure and high-fat diet could induce kidney damage through histopathological change and increased creatinine levels and kidney MDA levels in Wistar rats.

scholarly journals The Comparison of Bay Leaf and Celery Leaf Infusion Effect on Decreasing LDL Level in Dyslipidemic Wistar Rats Model

1970 ◽  
Vol 1 (1) ◽  
pp. 21
Author(s):  
Anjani Putri Retnaninggalih ◽  
Erfan Efendi ◽  
Hairrudin Hairrudin
Keyword(s):  
Cholesterol Level ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Ldl Cholesterol ◽  
Wistar Rat ◽  
Positive Control ◽  
Negative Control ◽  
High Fat ◽  
Leaf P ◽  
One Way Anova

Coronary heart disease (CHD) is the cause of 7,3 million death globally in 2008. The main pathophysiology process that leads to CHD is atherosclerosis which is caused by high LDL cholesterol level. The aim of this study was to explore bay leaf and celery leaf infusion effect on reducing LDL cholesterol level in dyslipidemic rat model. The type of this study was true experimental research using wistar rat (Rattus novergicus) as the sample. The rats were induced by high fat diet and PTU for two weeks then each of the group were given either bay leaf infusion, celery leaf infusion, simvastatin (positive control), or aquadest (negative control) for a week. The measurement of LDL level used Friedewald formulation. Data was analyzed using paired sample t test and one way ANOVA. As the result there was a significance reduction on LDL level after treatment with bay leaf (p=0,013) and celery leaf infusion (p=0,035) but there was no significance difference between groups (p=0,293). It was concluded that bay leaf and celery leaf infusion could reduce the LDL level in dyslipidemic wistar rat but there was no difference on the capability of the two different leafs on reducing LDL level. Keywords: dyslipidemia, LDL, bay leaf, celery leaf, wistar rats

scholarly journals Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia

2020 ◽  
Vol 1 (1) ◽  
pp. 25-30
Author(s):  
Melita Hidajat ◽  
I Gusti Made Aman ◽  
Hendro Sukoco ◽  
Ferbian Milas Siswanto
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Before And After ◽  
Male Wistar Rats ◽  
Guazuma Ulmifolia

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

scholarly journals Cell proliferation and apoptosis in Wistar rat kidney after renal mass ablation and low-dose irradiation

Medicina ◽  
2010 ◽  
Vol 46 (3) ◽  
pp. 204
Author(s):  
Marina Aunapuu ◽  
Andres Arend ◽  
Mai Ots ◽  
Mara Pilmane
Keyword(s):  
Cell Proliferation ◽  
Wistar Rats ◽  
Low Dose ◽  
Rat Kidney ◽  
Wistar Rat ◽  
Control Group ◽  
Dose Irradiation ◽  
Proliferation And Apoptosis ◽  
Male Wistar Rats ◽  
Distal Tubules

Cell proliferation and apoptosis in the remnant rat kidney after treatment with lowdose irradiation was investigated. Material and methods. In the first group (n=9), adult male Wistar rats underwent 5/6 nephrectomy (NPX); in the second group (n=9), NPX was combined with low-dose irradiation. Rats without surgery and irradiation formed the control group (n=9). Results. Hypertension and proteinuria induced by NPX were decreased by 3-Gy irradiation. The 5/6 NPX rats showed a dramatic increase in proliferating and apoptotic cells in the glomeruli and in the distal tubules at week 2, which was significantly decreased by low-dose irradiation. Conclusion. The data demonstrate that low-dose irradiation is a factor slowing the process of chronic renal injury.

scholarly journals A High-Fat Diet Containing 50% Egg-Phosphatidylcholine Increased the Proportion of T Cells Expressing a Memory Marker in Male Wistar Rats

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1140-1140
Author(s):  
Tianna Rusnak ◽  
Jessy Azarcoya Barrera ◽  
Bethany Wollin ◽  
Anna Thomsen ◽  
Alexander Makarowski ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Dose Effect ◽  
Helper T Cells ◽  
High Fat ◽  
Low Fat ◽  
Male Wistar Rats ◽  
Egg Pc

Abstract Objectives Studies have suggested that high-fat (HF) diets are associated with immune dysfunction, which results in a lower production of IL-2 and a lower proportion of helper T cells. Providing a diet containing 100% phosphatidylcholine (PC), a form of choline mainly found in eggs, has been shown to increase IL-2 production early in life. However, this is of no relevance for human consumption since no human diet will contain 100% PC. Therefore, the objective of this study was to determine the dose effect of egg-PC added to a high fat diet compared to a control high fat and low fat diets on T cell function in male Wistar rats. Methods At four weeks of age, male Wistar rats were randomized to consume one of 6 diets: 1- Control low fat (CLF, 10%wt/wt fat, 100% free choline (FC), n = 10); 2- Control high fat (CHF, 25% wt/wt fat, 100% FC, n = 10); 3- 100% PCHF (100PCHF, 25% wt/wt fat, 100% PC, n = 10); 4- 75% PCHF (75PCHF, 25% wt/wt fat, 75% PC, 25% FC, n = 10); 5- 50% PCHF (50PCHF, 25% wt/wt fat, 50% PC, 50% FC, n = 10); 6- 25% PCHF (25PCHF; 25% wt/wt fat, 25% PC, 75% FC, n = 10). Fatty acid composition was closely matched in all of the diets. Anthropometric data was collected through the duration of the study (12 weeks). At the end of the study, splenocytes phenotypes were measured by flow cytometry. Results From week 1 to week 10 there was no difference in body weight between the diets. Starting from week 2 the CLF group had a higher food intake compared to the other groups. The 50PCHF diet had a higher proportion of helper T cells (CD4+) compared to the CLF and CHF diets. In addition, 50PCHF had a higher proportion of helper T cells expressing IL-2 receptors (CD4+CD25+) compared to 25PCHF (P &lt; 0.05). 50PCHF also had a higher proportion of T cells expressing a memory marker (CD3+CD27+) compared with all HF diets (all P &lt; 0.05) but not the CLF diet. Conclusions Our results suggest that a diet providing 50% of total choline in the form of egg-PC normalizes the proportion of T cells expressing CD27 in the context of a HF diet which may lead to a better immune response if a second exposure to a pathogen occurs. Whether the higher proportion of helper T cells expressing the IL-2 receptor in the 50PCHF group is associated with better T cell response upon challenge remains to be investigated. Funding Sources Egg farmers of Canada, NSERC.

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