Interactions between field populations of the potato cyst nematode Globodera pallida and Rhizoctonia solani diseases of potatoes under controlled environment and glasshouse conditions

Nematology ◽  
2010 ◽  
Vol 12 (5) ◽  
pp. 783-790 ◽  
Author(s):  
Shuvash Bhattarai ◽  
Patrick P.J. Haydock ◽  
Matthew A. Back ◽  
Martin C. Hare ◽  
William T. Lankford

Abstract This research elucidates interactions between Globodera pallida and Rhizoctonia solani (AG3) by comparing disease development in potato plants inoculated with G. pallida and R. solani. The effects of juvenile population density and time of infestation on R. solani disease were investigated in controlled environments. Plants were inoculated with a range of densities of second-stage juveniles (J2) of G. pallida (800, 4000, 10 000, 20 000 and 40 000 J2/plant) and R. solani at 2, 4 and 6 weeks after planting and a single treatment of G. pallida density and R. solani alone. The experiment demonstrated that greatest disease incidence and severity occurred when high population densities of G. pallida were applied at an early stage of plant development. To determine if this effect could explain differences in R. solani disease incidence and severity following interactions with individual field populations of G. pallida, a glasshouse experiment was conducted using two populations of G. pallida with known hatching characteristics (fast and slow hatching). The experiment revealed that a combination of a fast-hatching population with R. solani caused significantly more R. solani disease incidence and severity than a population of G. pallida with slower hatching characteristics.

2010 ◽  
Vol 63 ◽  
pp. 133-137
Author(s):  
D. Bienkowski ◽  
A. Stewart ◽  
R.E. Falloon ◽  
M. Braithwaite ◽  
L.L. Loguercio ◽  
...  

Rhizoctonia solani is an important soilborne pathogen of potato causing black scurf on tubers and shoot/stolon canker on young plants These diseases reduce the quality of fresh and seed potatoes and may harm crop establishment A pathogenicity test has been developed for the study of biological suppression of diseases caused by R solani Disease expression from two R solani isolates was assessed in potato plants grown from tissue cultured plantlets or from minitubers The isolates were cultured in two media branwater or barley grainV8 juice which were compared as inoculants of potting mix at several standardised rates The proportions of diseased stolons and necrotic shoots were determined after 7 weeks growth in a greenhouse Significantly different levels of disease incidence were found between the isolates between inoculum concentrations for one isolate and (only with minitubers) between inoculum media Rates of inoculum that produced disease levels suitable for use in biocontrol assays were determined


Author(s):  
Anna Langstroff ◽  
Marc C. Heuermann ◽  
Andreas Stahl ◽  
Astrid Junker

AbstractRising temperatures and changing precipitation patterns will affect agricultural production substantially, exposing crops to extended and more intense periods of stress. Therefore, breeding of varieties adapted to the constantly changing conditions is pivotal to enable a quantitatively and qualitatively adequate crop production despite the negative effects of climate change. As it is not yet possible to select for adaptation to future climate scenarios in the field, simulations of future conditions in controlled-environment (CE) phenotyping facilities contribute to the understanding of the plant response to special stress conditions and help breeders to select ideal genotypes which cope with future conditions. CE phenotyping facilities enable the collection of traits that are not easy to measure under field conditions and the assessment of a plant‘s phenotype under repeatable, clearly defined environmental conditions using automated, non-invasive, high-throughput methods. However, extrapolation and translation of results obtained under controlled environments to field environments is ambiguous. This review outlines the opportunities and challenges of phenotyping approaches under controlled environments complementary to conventional field trials. It gives an overview on general principles and introduces existing phenotyping facilities that take up the challenge of obtaining reliable and robust phenotypic data on climate response traits to support breeding of climate-adapted crops.


Plant Disease ◽  
2007 ◽  
Vol 91 (10) ◽  
pp. 1305-1309 ◽  
Author(s):  
Khalil I. Al-Mughrabi ◽  
Rick D. Peters ◽  
H. W. (Bud) Platt ◽  
Gilles Moreau ◽  
Appanna Vikram ◽  
...  

The efficacy of metalaxyl-m (Ridomil Gold 480EC) and phosphite (Phostrol) applied at planting in-furrow against pink rot (Phytophthora erythroseptica) of potato (Solanum tuberosum) ‘Shepody’ and ‘Russet Burbank’ was evaluated in field trials conducted in 2005 and 2006 in Florenceville, New Brunswick, Canada. Inoculum made from a metalaxyl-m-sensitive isolate of P. erythroseptica from New Brunswick was applied either in-furrow as a vermiculite slurry at planting or as a zoospore drench in soils adjacent to potato plants in late August. After harvest, the number and weight of tubers showing pink rot symptoms were assessed and expressed as percentages of the total tuber number and total weight of tubers. Metalaxyl-m applied in-furrow was significantly more effective against pink rot than phosphite. The mean percentage of diseased tubers as a percentage of total tuber weight was 1.5% (2005) and 1.2% (2006) for metalaxyl-m-treated plots and 9.6% (2005) and 2.8% (2006) for phosphite-treated plots, a percentage similar to that obtained in inoculated control plots with no fungicide treatment. The mean percentage of diseased tubers expressed as a percentage of the total number of tubers was 1.7% (2005) and 1.3% (2006) for metalaxyl-m-treated plots and 10.1% (2005) and 3.1% (2006) for phosphite-treated plots. Disease incidence was significantly higher using the late-season inoculation technique (respective means in 2005 and 2006 were 9.9 and 3.8% diseased tubers, by weight, and 10.6 and 3.9%, by number) than with the in-furrow inoculation method (respective means in 2005 and 2006 were 3.3 and 0.7% by weight, and 3.7 and 1.3%, by number). The potato cv. Shepody was significantly more susceptible to pink rot (9.9 and 3.3% diseased tubers, by weight, in 2005 and 2006, respectively, and 10.6 and 3.9%, by number) than Russet Burbank (respective means in 2005 and 2006 were 3.4,% and 1.2%, by weight, and 3.7,% and 1.2%, by number). Our findings indicate that metalaxyl applied in-furrow at planting is a viable option for control of pink rot caused by metalaxyl-sensitive strains of P. erythroseptica, whereas phosphite was ineffective.


Plant Disease ◽  
2015 ◽  
Vol 99 (12) ◽  
pp. 1790-1802 ◽  
Author(s):  
N. Muzhinji ◽  
M. Truter ◽  
J. W. Woodhall ◽  
J. E. van der Waals

A survey of anastomosis groups (AG) of Rhizoctonia spp. associated with potato diseases was conducted in South Africa. In total, 112 Rhizoctonia solani and 19 binucleate Rhizoctonia (BNR) isolates were recovered from diseased potato plants, characterized for AG and pathogenicity. The AG identity of the isolates was confirmed using phylogenetic analysis of the internal transcribed spacer region of ribosomal DNA. R. solani isolates recovered belonged to AG 3-PT, AG 2-2IIIB, AG 4HG-I, AG 4HG-III, and AG 5, while BNR isolates belonged to AG A and AG R, with frequencies of 74, 6.1, 2.3, 2.3, 0.8, 12.2, and 2.3%, respectively. R. solani AG 3-PT was the most predominant AG and occurred in all the potato-growing regions sampled, whereas the other AG occurred in distinct locations. Different AG grouped into distinct clades, with high maximum parsimony and maximum-likelihood bootstrap support for both R. solani and BNR. An experiment under greenhouse conditions with representative isolates from different AG showed differences in aggressiveness between and within AG. Isolates of AG 2-2IIIB, AG 4HG-III, and AG R were the most aggressive in causing stem canker while AG 3-PT, AG 5, and AG R caused black scurf. This is the first comprehensive survey of R. solani and BNR on potato in South Africa using a molecular-based approach. This is the first report of R. solani AG 2-2IIIB and AG 4 HG-I causing stem and stolon canker and BNR AG A and AG R causing stem canker and black scurf on potato in South Africa.


Author(s):  
Т.А. Богумил

В статье предлагается концепция жизни и творческого роста В.М. Шукшина сквозь призму тезаурусного и кластерного подходов. Объяснительными «персональными моделями» для интерпретации биографического текста писателя являются кластеры Мартина Идена, Гамлета и Степана Разина. Ранее биографы и литературоведы указывали на важность этих персон для Шукшина, но разрозненно. Впервые предпринята попытка свести указанные персональные модели воедино, в целостный сюжет становления писателя в пространстве культуры. На разных этапах эволюции автора доминирует одна из указанных моделей, последовательно сменяя друг друга, но не вытесняя, а «внахлест». Ранний этап творчества, ориентированный на стратегию Мартина Идена, является попыткой «вписаться» в наличный культурный мейнстрим. Выражено это в следовании поэтике соцреализма. Второй этап подспудно начинается с реабилитации отца Шукшина в 1956 года, когда актуализируется «гамлетовский комплекс», проявляющийся в чувстве вины перед отцом, через стратегию утаивания подлинного «я», мотивы чудаковатости. Третий этап связан с личностью реального исторического лица, Степана Разина, и выражается в открытом протесте против власти. Все важные для В.М. Шукшина поведенческие модели объединены мотивом одинокого противостояния враждебному социуму и имеют трагический финал, что обусловило ранний уход писателя и порождение мифов о насильственном характере его смерти. В.М. Шукшин одновременно был фигурой неординарной и типичной, что позволило его биографии стать «персональной моделью» для последующих авторов, выходцев из сельской глубинки. The article proposes the concept of life and creative growth of V.M. Shukshin through the prism of the thesaurus and cluster approach. The explanatory "personal models" for interpreting the biographical text of the writer are clusters of Martin Eden, Hamlet and Stepan Razin. Earlier, biographers and literary critics pointed out the importance of these people for Shukshin, but it was scattered. For the first time, an attempt was made to bring these personal models together into an integral plot of the formation of the writer in the space of culture. At different stages of the writer’s evolution, one of these models dominates, successively replacing each other, but not crowding out, but “overlapping”. The early stage of creativity, focused on Martin Eden’s strategy, is an attempt to “fit in” the current cultural mainstream. This is expressed in following the poetics of socialist realism. The second stage implicitly begins with the rehabilitation of Shukshin’s father in 1956, when the “Hamletian complex” is actualized. It is manifested in a sense of guilt towards his father, the strategy of concealing the true “I”, eccentricities. The third stage is connected with the personality of a real historical person, Stepan Razin, and is expressed in an open protest against the authorities. All important for V.M. Shukshin's behavioral models are united by the motive of a lonely confrontation with a hostile society and have a tragic ending, which led to the early departure of the writer and the generation of myths about the violent nature of his death. V.M. Shukshin was an extraordinary and typical figure at the same time. That allowed his biography to become a “personal model” for subsequent authors who came from the rural outback.


2004 ◽  
Vol 126 (5) ◽  
pp. 925-931 ◽  
Author(s):  
Yanyao Jiang ◽  
Ming Zhang ◽  
Tae-Won Park ◽  
Chu-Hwa Lee

The self-loosening process of a bolted joint consists of two distinct stages. The early stage of self-loosening is due to the cyclic plastic deformation of the materials. The second stage of self-loosening is characterized by the backing off of the nut. The current work is concentrated on an experimental investigation of the second stage self-loosening. Over one hundred bolted joints with M12×1.75 bolts and nuts were experimentally tested using a specially designed testing apparatus. The experiments mimicked two plates jointed by a bolt and a nut and were subjected to cyclic transverse shear loading. During an experiment, the relative displacement between the two clamped plates, denoted by δ, was a controlling parameter. For a given preload, the relationship between, Δδ/2, the amplitude of the relative displacement between the two clamped plates, and, NL, the number of loading cycles to loosening followed a pattern similar to a fatigue curve. There existed an endurance limit below which self-loosening would not persist. A larger preload resulted in a larger endurance limit. However, a large preload increased the possibility for the bolt to fail in fatigue. The results suggest that the use of a regular nut is superior to the use of a flange nut in terms of self-loosening resistance.


Plant Disease ◽  
2010 ◽  
Vol 94 (1) ◽  
pp. 125-125 ◽  
Author(s):  
G. Polizzi ◽  
D. Aiello ◽  
I. Castello ◽  
V. Guarnaccia ◽  
A. Vitale

Mediterranean fan palm (Chamaerops humilis L.), one of just two autochthonous European palms, is native to the western Mediterranean Region in southwestern Europe and northwestern Africa. It can be found growing wild in the Mediterranean area. In Europe, this species is very popular as an ornamental plant. In March 2009, a widespread damping-off was observed in a stock of approximately 30,000 potted 1-month-old plants of C. humilis cv. Vulcano in a nursery in eastern Sicily. Disease incidence was approximately 20%. Disease symptoms consisted of lesions at the seedling shoot (plumule). Stem lesions were initially orange, turned brown, and followed by death of the entire plumule or eophyll. A fungus with mycelial and morphological characteristics of Rhizoctonia solani Kühn was consistently isolated from lesions when plated on potato dextrose agar (PDA) amended with streptomycin sulfate at 100 μg/ml. Fungal colonies were initially white, turned brown with age, and produced irregularly shaped, brown sclerotia. Mycelium was branched at right angles with a septum near the branch and a slight constriction at the branch base. Hyphal cells removed from cultures grown at 25°C on 2% water agar were determined to be multinucleate when stained with 1% safranin O and 3% KOH solution (1) and examined at ×400. Anastomosis groups were determined by pairing isolates with tester strains AG-1 IA, AG-2-2-1, AG-2-2IIIB, AG-2-2IV, AG-3, AG-4, AG-5, AG-6, and AG-11 on 2% water agar in petri plates (3). Anastomosis was observed only with tester isolates of AG-4, giving both C2 and C3 reactions (2). One representative isolate obtained from symptomatic tissues was deposited at the Fungal Biodiversity Centre, Centraalbureau voor Schimmelcultures (CBS No. 125095). Pathogenicity tests were performed on container-grown, healthy, 1-month-old seedlings. Twenty plants of C. humilis cv. Vulcano were inoculated near the base of the stem with two 1-cm2 PDA plugs from 5-day-old mycelial cultures. The same number of plants served as uninoculated controls. Plants were incubated in a growth chamber and maintained at 25°C and 95% relative humidity on a 12-h fluorescent light/dark regimen. Symptoms identical to those observed in the nursery appeared 5 days after inoculation and all plants died within 20 days. No disease was observed on control plants. A fungus identical in culture morphology to R. solani AG-4 was consistently reisolated from symptomatic tissues, confirming its pathogenicity. To our knowledge, this is the first report in the world of R. solani causing damping-off on Mediterranean fan palm. References: (1) R. J. Bandoni. Mycologia 71:873, 1979. (2) D. E. Carling. Page 37 in: Grouping in Rhizoctonia solani by Hyphal Anastomosis Reactions. Kluwer Academic Publishers, the Netherlands, 1996. (3) C. C. Tu and J. W. Kimbrough. Mycologia 65:941, 1973.


Author(s):  
Narendra Singh ◽  
Vikee M. Patel

Black scurf (Rhizoctonia solani) disease of potato is becoming prominent in many potato growing districts of Gujarat state which reduce quality and market value of the produce, resulting in economic losses. The field experiment was conducted during rabi season of 2014-15 in naturally infested field with scurf pathogen at Potato Research Station, S.D. Agricultural University, Deesa (Gujarat) with the objective to find out suitable management strategies for black scurf through organic approaches (bio-agents and organic amendments). Among different bio-agents (Trichoderma viride, T. harzianum. Pseudomonas fluorescens and Bacillus subtilis) tested against black scurf, the tuber treatment with 2 per cent boric acid spray along with tuber treatment with T. viride @ 10 g/kg seed before sowing recorded the lowest disease incidence (15.33 %) and index (0.38) with highest total tuber yield (324.68 q/ha) with maximum income `2,57,414/ha. When price computed with healthy and diseased tuber yield among treatments, the maximum income variation was (`92,986/ha) recorded by the same treatment followed by the tuber treatment with 3 % boric acid spray before sowing (`68,440/ha). In case of various organic amendments tested, soil application of mustard cake @ 10 q/ha registered minimum disease incidence (37.33 %) and disease index (0.66) as compared to rest of the organic treatment with highest total tuber yield (`327.20 q/ha) with maximum income `2,65,010/ha. When price computed with healthy and diseased tuber yield among treatments, the maximum income variation was (`1,12,990/ha) recorded by the same treatment followed by the neem cake @ 10 q/ha (`56,660/ha). These organic treatments can provide an effective and economical management of black scurf of potato for cultivaters.


2016 ◽  
Vol 2 (1) ◽  
pp. 77-89 ◽  
Author(s):  
Jorge A. Llontop ◽  
María M. Scurrah ◽  
Javier Franco

Desde 1980 el Centro Internacional de la Papa (CIP) ha estado enviando clones de papa a varios investigadores del Programa Nacional de Papa del Perú adscrito al Instituto Nacional de Investigación Agraria y Agroindustrial (INIAA), para evaluar su comportamiento y resistencia al nemátodo quiste de la papa con el objeto de seleccionar variedades resistentes. En 1983, el CIPA IV del INIAA, en La Libertad (Sierra Norte del Perú), seleccionó un clon sobresaliente, identificado como 279142.12 ó G3. Después de varias pruebas, el clon fue liberado en 1987 con el nombre de María Huanca, y es la primera variedad resistente a las razas P4A y P5A de G. pallida en el Perú y Latinoamérica. Esta variedad proviene de un cruce entre S. tuberosum subsp. andigena y un híbrido de 5. tuberosum subsp. tuberosum x S. vernei. Los tubérculos son oblongos, de piel rojiza, la pulpa es blanca, ocasionalmente con estrías moradas. La planta es erecta y alcanza una altura de 80 cm con pequeños folíolos de color verde oscuro; tiene el fenotipo de andigena. Los rendimientos de las Estaciones Experimentales variaron entre 30 y 60 t/ha y en campos de agricultores de 20 a 30 t/ha. Además de su resistencia al nemátodo del quiste de la papa, esta variedad es también resistente a las razas 1 y 2 de la verruga (Synchitrium endobioticum), inmune a PVY e hipersensitiva a la raza común de PVY. Es tolerante a rancha (Phytophthora infestans) y al carbón de la papa (Tecaphora solani); susceptible a roña (Spongospora subterranea),rizoctoniasis (Rhizoctonia solani), oidium (Erysiphe cichoracearum) y a mancha foliar (Phoma andigena).


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