Chorion exploration in the tribe Anthocharidini (Lepidoptera: Pieridae) and their possible importance in its systematics

Zootaxa ◽  
2020 ◽  
Vol 4868 (2) ◽  
pp. 151-207
Author(s):  
SANDRA NIEVES-URIBE ◽  
ADRIÁN FLORES-GALLARDO ◽  
JORGE LLORENTE-BOUSQUETS
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Costa Rica ◽  
Structural Features ◽  
Bibliographic Record ◽  
Scanning Electron

We describe and compare the morphology of the chorion in nine species belonging to five genera of the tribe Anthocharidini (Pieridae: Pierinae), from a sample of 12 females with mature eggs, the bibliographic record of oviposited eggs, and photographs of scanning electron microscopy (SEM). The eggs examined come from Mexico, Spain, Brazil and Costa Rica. Its characterization was made considering the main structural features of the chorion in this tribe; it includes a distinction of structures in the apical zone and differentiation between the chorionic regions. We attached to this work sheets, diagrams, and terminology to understand and clarify the descriptions. Our results agree with the proposal of Anthocharidini as the least derived tribe of the Pierinae, considering that Hebomoia, a specialized genus, is not part of it. Tribes such as Leptosiaini, Elodinini or Nepheroniini also have more chorionic characteristics related to more derived tribes of the Pierinae. 

Wood identification of charcoal with 3D-reflected light microscopy

IAWA Journal ◽  
2020 ◽  
Vol 41 (4) ◽  
pp. 478-489 ◽  
Author(s):  
Valentina Zemke ◽  
Volker Haag ◽  
Gerald Koch
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Field Emission ◽  
Polarized Light ◽  
Structural Features ◽  
Light Illumination ◽  
Reflected Light ◽  
Carbonized Wood ◽  
Scanning Electron

Abstract The present study focusses on the application of 3D-reflected light microscopy (3D-RLM) for the wood anatomical identification of charcoal specimens produced from domestic and tropical timbers. This special microscopic technique offers a detailed investigation of anatomical features in charcoal directly compared with the quality of field emission scanning electron microscopy (FESEM). The advantages of using the 3D-RLM technology are that fresh fracture planes of charcoal can be directly observed under the microscope without further preparation or surface treatment. Furthermore, the 3D-technique with integrated polarized light illumination creates high-contrast images of uneven and black charcoal surfaces. Important diagnostic structural features such as septate fibres and intercellular canals can be clearly detected and intervessel pits are directly measured. The comparison of the microscopic analyses reveals that 3D-reflected light microscopy (3D-RLM) provides an effective alternative technique to conventional field emission scanning electron microscopy for the identification of carbonized wood.

Cobalt Based Alloys for Dental Applications

2013 ◽  
Vol 583 ◽  
pp. 175-178 ◽  
Author(s):  
Brandusa Ghiban ◽  
Cristina Maria Borţun ◽  
Alexandru Ghiban ◽  
Nicolae Ghiban
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Mechanical Behaviour ◽  
Structural Features ◽  
Molybdenum Content ◽  
Cobalt Alloys ◽  
Mo Content ◽  
Dental Applications ◽  
Scanning Electron

The present paper put in evidence the mechanical behaviour of some commercial dental cobalt alloys, after own classification. The experimental cobalt alloys were classified in according either with molybdenum content, or with Cr+Mo content. A hierarcy of the alloys was finally made. The fractographic surfaces were investigate both by stereomacroscopy and scanning electron microscopy, putting in evidence the main structural features.

Field Emission Scanning Electron Microscopy Studies of Industrial Polymers - A Survey

1998 ◽  
Vol 4 (S2) ◽  
pp. 814-815
Author(s):  
E.F. Osten ◽  
M.S. Smith
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Field Emission ◽  
Low Voltage ◽  
Structural Features ◽  
X Ray Diffraction ◽  
Styrene Acrylonitrile ◽  
Microscopy Techniques ◽  
Scanning Electron

We are using the term "Industrial Polymers" to refer to polymers [plastics] that are produced by the ton or (in the case of films) by the mile. For example, in descending order of world-wide use (tonnage), the top eight of these polymers are polyethylene (PE), polyvinyl chloride (PVC), polypropylene (PP), styrene polymers (including polystyrene - PS, and acrylonitrile-butadienestyrene/ styrene-acrylonitrile - ABS/SAN), polyesters (PETP), polyurethane (PU), phenolics and aminoplastics.Industrial polymers, which have been produced by the millions of tons for the last five decades and are of obvious social and economic importance, have been exhaustively characterized. Structural features which affect physical properties and indicate process variables have been studied by many techniques other than microscopy (x-ray diffraction, thermal analysis, rheology, chromatographies, etc.). Microscopy techniques for polymer characterization have been well documented. Our motivation to apply field emission (high resolution) scanning electron microscopy to the study of polymers is: (1) The application of low voltage, high resolution SEM to biological materials is well characterized.

scholarly journals Use of different SEM techniques in the study of Tyrophagus putrescentiae (Acari: Acaridae) in Costa Rica

2013 ◽  
Vol 5 (2) ◽  
pp. 201-208
Author(s):  
Pamela Murillo ◽  
Hugo Aguilar ◽  
Ethel Sanchez
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Costa Rica ◽  
Morphological Characters ◽  
Morphological Description ◽  
Tyrophagus Putrescentiae ◽  
Viable Option ◽  
Efficient Procedure ◽  
Advantages And Disadvantages ◽  
Scanning Electron

Scanning electron microscopy (SEM) has been used as a tool to facilitate the morphological description of mites and to complement the information obtained from the light microscope. Unfortunately, several of SEM techniques used for soft-bodied mites such as Tyrophagus, have in many cases not shown the desired results. This investigation sought to develop an efficient procedure for preparing specimens of T. putrescentiae for SEM so that its major morphological characters could be observed and photographed. This species is the most important contaminant of tissue culture laboratories in Costa Rica. Seven methods were used to process the samples. The evaluated treatments showed differences in their ability to preserve the morphological structures of the specimens. The advantages and disadvantages of each treatment are discussed.  Mites processed using ethanol were the most viable option with method 3 being the procedure that showed the best results; this technique also reduced the time and costs of processing the specimens.KEY WORDSAcari, Acaridae, Tyrophagus putrescentiae, Scanning Electron Microscopy (SEM), SEM techniques

scholarly journals Automated Fiber Diameter and Porosity Measurements of Plasma Clots in Scanning Electron Microscopy Images

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1536
Author(s):  
Ali Daraei ◽  
Marlien Pieters ◽  
Stephen R. Baker ◽  
Zelda de Lange-Loots ◽  
Aleksander Siniarski ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fiber Diameter ◽  
Structural Features ◽  
Fibrin Clot ◽  
Depth Of Field ◽  
Pixel Size ◽  
Sem Images ◽  
Highly Correlated ◽  
Scanning Electron

Scanning Electron Microscopy (SEM) is a powerful, high-resolution imaging technique widely used to analyze the structure of fibrin networks. Currently, structural features, such as fiber diameter, length, density, and porosity, are mostly analyzed manually, which is tedious and may introduce user bias. A reliable, automated structural image analysis method would mitigate these drawbacks. We evaluated the performance of DiameterJ (an ImageJ plug-in) for analyzing fibrin fiber diameter by comparing automated DiameterJ outputs with manual diameter measurements in four SEM data sets with different imaging parameters. We also investigated correlations between biophysical fibrin clot properties and diameter, and between clot permeability and DiameterJ-determined clot porosity. Several of the 24 DiameterJ algorithms returned diameter values that highly correlated with and closely matched the values of the manual measurements. However, optimal performance was dependent on the pixel size of the images—best results were obtained for images with a pixel size of 8–10 nm (13–16 pixels/fiber). Larger or smaller pixels resulted in an over- or underestimation of diameter values, respectively. The correlation between clot permeability and DiameterJ-determined clot porosity was modest, likely because it is difficult to establish the correct image depth of field in this analysis. In conclusion, several DiameterJ algorithms (M6, M5, T3) perform well for diameter determination from SEM images, given the appropriate imaging conditions (13–16 pixels/fiber). Determining fibrin clot porosity via DiameterJ is challenging.

Fabrication of Electrochemical Sensors Based on Nanostructured Titanium Dioxide Formed through Anodic Oxidation

2015 ◽  
Vol 245 ◽  
pp. 182-189 ◽  
Author(s):  
Nikolai B. Kondrikov ◽  
Antonina S. Lapina ◽  
Ilya V. Stepanov ◽  
Galina I. Marinina ◽  
Vladimir V. Korochentsev ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Titanium Dioxide ◽  
Anodic Oxidation ◽  
Photoelectron Spectroscopy ◽  
Electrochemical Sensors ◽  
Structural Features ◽  
X Ray ◽  
Different Types ◽  
Scanning Electron

The nanotubular titanium dioxide structures were prepared using anodic oxidation. The structural features of surface have been investigated by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and energy-dispersive spectroscopy (EDS) techniques. These nanotubular titanium dioxide structures can be used as a sensor in potentiometric indication components of different types of chemical reactions.

Lophodermium pinastri: certain fine-structural features of ascocarp morphology

1974 ◽  
Vol 52 (8) ◽  
pp. 1993-1995 ◽  
Author(s):  
B. A. Silverberg ◽  
J. F. Morgan-Jones
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Electron Density ◽  
Structural Features ◽  
Acid Mucopolysaccharide ◽  
Scanning Electron ◽  
Mucilaginous Material

As viewed by the electron microscope, the 'carbonaceous' appearance of the ascocarp clypeus in Lophodermium pinastri (Schrad) Chev. is due to a dark substance in the interhyphal spaces; this substance is believed to originate within the hyphae since the latter contain dark inclusions of the same electron density. The mucilaginous material in the ascocarp cavity is primarily in the nature of an acid mucopolysaccharide, together with minimal amounts of carbohydrates and proteins. Examination of the interthecial hyphae by means of scanning electron microscopy (SEM) shows them to be true paraphyses, not pseudoparaphyses as previously supposed.

A new species of Cymbella (Cymbellaceae, Bacillariophyceae) from China, possessing valves with both uniseriate and biseriate striae

Phytotaxa ◽  
2018 ◽  
Vol 344 (1) ◽  
pp. 39 ◽  
Author(s):  
BING LIU ◽  
DAVID M. WILLIAMS ◽  
QINGYU LIU
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Species ◽  
Structural Features ◽  
Freshwater Habitats ◽  
A New Species ◽  
Scanning Electron

Since scanning electron microscopy was used to study diatoms, many new ultra-structural features have been observed. This paper describes Cymbella distalebiseriata, a new species of Cymbella which has valves with biseriate striae near both apices of the valve and uniseriate striae at the valve centre. Comparison with some similar taxa supports its erection as new by the following combination of characters: two to three stigmata, puncta density 25–30 in 10 μm, and ca. 3 to 11 biseriate striae at both apices. C. distalebiseriata is epilithic and lives in freshwater habitats.

Transfer of Fragilaria atomus Hust. to the genus Stauroforma (Bacillariophyta) based on observation of type and newly collected material

Phytotaxa ◽  
2014 ◽  
Vol 158 (1) ◽  
pp. 43 ◽  
Author(s):  
Dávia Talgatti ◽  
Carlos E. Wetzel ◽  
Eduardo A. Morales ◽  
Luc Ector ◽  
Lezilda Carvalho Torgan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salt Marshes ◽  
Brackish Water ◽  
Structural Features ◽  
Apical Pore ◽  
Distribution And Ecology ◽  
Scanning Electron

Fragilaria atomus was described from a brackish water lagoon in southern Finland and has subsequently been reported from several localities worldwide. However, due to its small size, it can be easily mistaken with other small, morphologically similar araphid diatoms. To clarify the morphological, metric and structural features of the species, lectotype material from BRM (Hustedt’s diatom collection) and specimens from salt marshes in Brazil were studied using light and scanning electron microscopy. Fragilaria atomus is compared to seven morphologically similar taxa belonging to Fragilaria, Stauroforma and Psammoneis. The results revealed that some important features of Fragilaria (discoid closing plates, rimoportulae and spines) are not present in F. atomus. The absence of these structures, together with the opposite striation pattern, round areolae, features of the apical pore field, and reduced or absent sternum, suggest that a transfer of F. atomus to Stauroforma is appropriate. The distribution and ecology of F. atomus is discussed.

Mechanical and Corrosion Behaviour of Some Devices for Ostheosinthesis

2007 ◽  
Vol 23 ◽  
pp. 257-260 ◽  
Author(s):  
Brandusa Ghiban
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Femoral Fracture ◽  
Soft Tissues ◽  
Corrosion Behaviour ◽  
Structural Features ◽  
X Rays ◽  
Hard Tissues ◽  
Mechanical Devices ◽  
Scanning Electron

Orthopaedic implants represent mechanical devices which may be used for different purposes in human skeleton. such as either repairing of soft tissues (ligaments) or hard tissues (fractures. osteotomy. partial or total replacements). The aim of present paper is to put in evidence some macro and micro structural features revealed at state of surface after 5 months of implant in a female femoral fracture. Investigated surfaces were analyzed by optical and scanning electron microscopy and by X-Rays diffraction.

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