Extraction of Damba Black Soybean Gene by Means of Magnetic Nanoparticles and Investigation of Aluminum Tolerance

2021 ◽  
Vol 15 (2) ◽  
pp. 156-162
Author(s):  
Tao Lin ◽  
Rongrong Han ◽  
Yunmin Wei ◽  
Lusheng Liu ◽  
Caode Jiang ◽  
...  
Keyword(s):  
Glycine Max ◽  
Acid Secretion ◽  
Transgenic Tobacco ◽  
Citrate Synthase ◽  
Antioxidant Properties ◽  
Aluminum Tolerance ◽  
Root Tips ◽  
Black Soybean ◽  
Soybean Gene ◽  
Organic Acid Secretion

The aluminum tolerance of Tamba black soybean (Glycine max cv. Tamba) is closely related to organic acid secretion mechanisms. The gene responsible for this tolerance in this variety (GmFER84) is extracted from lysates of soybean root tips using silylated Fe3O4 nanomagnetic beads. GmFER84 (Glycine max XP 003540203.1) is a stable protein. Tobacco genetically transformed with GmFER84 using an Agrobacterium-mediated transformation was tested for aluminum tolerance. Citrate synthase and citric acid secretion in the roots of transgenic tobacco prove to be significantly higher than those of wild tobacco, and the antioxidant properties of transgenic tobacco are also substantially increased. Research on GmFER84 may enable further agronomic development.

One Novel Mitochondrial Citrate Synthase from Oryza sativa L. can Enhance Aluminum Tolerance in Transgenic Tobacco

2009 ◽  
Vol 42 (3) ◽  
pp. 299-305 ◽  
Author(s):  
Yingying Han ◽  
Wenzheng Zhang ◽  
Bailong Zhang ◽  
Shanshan Zhang ◽  
Wei Wang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Transgenic Tobacco ◽  
Citrate Synthase ◽  
Oryza Sativa L ◽  
Aluminum Tolerance

scholarly journals Metabolic acclimation supports higher aluminium-induced secretion of citrate and malate in an aluminium-tolerant hybrid clone of Eucalyptus

2020 ◽  
Author(s):  
Wannian Li ◽  
Patrick Finnegan ◽  
Qin Dai ◽  
Mei Yang
Keyword(s):  
Acid Secretion ◽  
Organic Acid ◽  
Citrate Synthase ◽  
Acid Soil ◽  
Anion Channel ◽  
Lag Phase ◽  
Protein Synthesis Inhibitor ◽  
Timber Species ◽  
Al Tolerance ◽  
Organic Acid Secretion

Abstract Background Eucalyptus is the main timber species, most of which are hybrid clones, and usually grow in aluminized acid soil in China. The exudation of organic acids from roots may contribute to detoxification of Al and lead to the Al-tolerance in Eucalyptus genotypes. To further understand the organic acid response in Al tolerance in Eucalyptus, the Al-tolerant Eucalyptus grandis × E. urophylla clone GL-9 (marked as “G9”) and the Al-sensitive Eucalyptus urophylla clone GL-4 (marked as “W4”) were used to investigate the secretion and metabolism of citrate and malate in roots. Results Eucalyptus seedlings in hydroponics were exposed to the presence or absence of 4.4 mM Al at pH 4.0 for 24 hours. The protein synthesis inhibitor cycloheximide (CHM) and the anion channel blocker phenylglyoxal (PG) were applied to explore possible pathways involved in organic acid secretion. The Al treatments caused higher Al accumulation in roots of both clones. The secretion of malate and citrate was greater in G9 than in W4, corresponding to the relatively higher tolerance in G9 to Al. The peak Al concentration occurred after 1 h in G9 roots and declined afterward, indicating the activation of detoxification to alleviate Al accumulation. After 6 h of Al exposure, the efflux of citrate dramatically increased in G9 after a substantial lag phase, while both peak Al accumulation in roots and peak malate secretion occurred and there was no induction of citrate secretion in W4. Enhanced activity for citrate synthase and phosphoenolpyruvate carboxylase, and reduced activity for NADP-isocitrate dehydrogenase, aconitase and NADP-malic enzyme were closely associated with the greater secretion of citrate in G9. Both PG and CHM were effective inhibitors of citrate and malate secretion in both Eucalyptus clones, except the malate secretion in W4 was not affected by CHM. Conclusions In two different Al-tolerant Eucalyptus clones, both secretion and internal accumulation of citrate and malate in roots were involved in Al detoxification. An anion channel on the plasma membrane could be an important mode of organic acid secretion. Citrate and relevant metabolizing enzymes led more important role in the response to Al in E. grandis × E. urophylla.

scholarly journals Enhanced citric acid biosynthesis in Pseudomonas fluorescens ATCC 13525 by overexpression of the Escherichia coli citrate synthase gene

Microbiology ◽  
2009 ◽  
Vol 155 (8) ◽  
pp. 2620-2629 ◽  
Author(s):  
Aditi D. Buch ◽  
G. Archana ◽  
G. Naresh Kumar
Keyword(s):  
Escherichia Coli ◽  
Citric Acid ◽  
Acid Secretion ◽  
Pseudomonas Fluorescens ◽  
Phosphoenolpyruvate Carboxylase ◽  
Citrate Synthase ◽  
Acid Biosynthesis ◽  
Acid Yield ◽  
Glucose Catabolism ◽  
Citric Acid Biosynthesis

Citric acid secretion by fluorescent pseudomonads has a distinct significance in microbial phosphate solubilization. The role of citrate synthase in citric acid biosynthesis and glucose catabolism in pseudomonads was investigated by overexpressing the Escherichia coli citrate synthase (gltA) gene in Pseudomonas fluorescens ATCC 13525. The resultant ∼2-fold increase in citrate synthase activity in the gltA-overexpressing strain Pf(pAB7) enhanced the intracellular and extracellular citric acid yields during the stationary phase, by about 2- and 26-fold, respectively, as compared to the control, without affecting the growth rate, glucose depletion rate or biomass yield. Decreased glucose consumption was paralleled by increased gluconic acid production due to an increase in glucose dehydrogenase activity. While the extracellular acetic acid yield increased in Pf(pAB7), pyruvic acid secretion decreased, correlating with an increase in pyruvate carboxylase activity and suggesting an increased demand for the anabolic precursor oxaloacetate. Activities of two other key enzymes, glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase, remained unaltered, and the contribution of phosphoenolpyruvate carboxylase and isocitrate lyase to glucose catabolism was negligible. Strain Pf(pAB7) demonstrated an enhanced phosphate-solubilizing ability compared to the control. Co-expression of the Synechococcus elongatus PCC 6301 phosphoenolpyruvate carboxylase and E. coli gltA genes in P. fluorescens ATCC 13525, so as to supplement oxaloacetate for citrate biosynthesis, neither significantly affected citrate biosynthesis nor caused any change in the other physiological and biochemical parameters measured, despite approximately 1.3- and 5-fold increases in citrate synthase and phosphoenolpyruvate carboxylase activities, respectively. Thus, our results demonstrate that citrate synthase is rate-limiting in enhancing citrate biosynthesis in P. fluorescens ATCC 13525. Significantly low extracellular citrate levels as compared to the intracellular levels in Pf(pAB7) suggested a probable limitation of efficient citrate transport.

scholarly journals Yak-Kong Soybean (Glycine max) Fermented by a Novel Pediococcus pentosaceus Inhibits the Oxidative Stress-Induced Monocyte–Endothelial Cell Adhesion

Nutrients ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1380 ◽  
Author(s):  
Ji Seung Kim ◽  
Jong Hun Kim ◽  
Sasikumar Arunachalam Palaniyandi ◽  
Charles C. Lee ◽  
Ji Woo You ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Adhesion ◽  
Glycine Max ◽  
Endothelial Cell ◽  
Phenolic Acids ◽  
Coumaric Acid ◽  
Antioxidant Ability ◽  
Pediococcus Pentosaceus ◽  
Black Soybean ◽  
Endothelial Cell Adhesion

Yak-Kong (YK), a small black soybean (Glycine max) in Korea, contained higher concentrations of antioxidants than ordinary black soybean or yellow soybean in our previous study. We prepared the fermented YK extract by using a novel lactic acid bacterium, Pediococcus pentosaceus AOA2017 (AOA2017) isolated from Eleusine coracana, and found that the antioxidant ability was enhanced after fermentation. In order to investigate the cause of the enhanced antioxidant ability in the fermented YK extract, we conducted a phenolic composition analysis. The results show that proanthocyanidin decreased and phenolic acids increased with a statistical significance after fermentation. Among the phenolic acids, p-coumaric acid was newly produced at about 11.7 mg/100 g, which did not exist before the fermentation. Further, the fermented YK extract with increased p-coumaric acid significantly inhibited the lipopolysaccharide-induced THP-1 monocyte–endothelial cell adhesion compared to the unfermented YK extract. The fermented YK extract also suppressed the protein expression levels of vascular cell adhesion molecule (VCAM)-1 in human umbilical vein endothelial cells (HUVECs). Together with the previous studies, our results suggest that the extract of YK fermented by AOA2017 has potential to be a new functional food material with its enhanced bioactive compounds which may help to prevent atherosclerosis caused by oxidative stress.

Metolachlor and Alachlor Effects on Membrane Permeability and Lipid Synthesis

Weed Science ◽  
1982 ◽  
Vol 30 (4) ◽  
pp. 399-404 ◽  
Author(s):  
Jill M. Mellis ◽  
Parthan Pillai ◽  
Donald E. Davis ◽  
Bryan Truelove
Keyword(s):  
Zea Mays ◽  
Glycine Max ◽  
Choline Chloride ◽  
Lipid Synthesis ◽  
Significant Loss ◽  
Phospholipid Synthesis ◽  
Root Tips ◽  
Naphthalic Anhydride ◽  
Cotton Root ◽  
High Concentrations

Metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide] at 1 × 10−5and 1 × 10−4M increased the leakage of previously absorbed,32P-labeled orthophosphate from the roots of onion (Allium cepaL.), a susceptible species, by 14 and 41 times the control values, respectively. A significant amount of32P leaked from the roots of the moderately susceptible species, cotton (Gossypium hirsutumL. ‘DPL 61′) and cucumber (Cucumis sativusL. ‘Ashley′), whereas no significant loss of32P occurred from two tolerant species, soybean [Glycine max(L.) Merr. ‘Bragg′] and corn (Zea maysL. ‘Pioneer 3369A′). At either 1 × 10−7or 1 × 10−6M, 1,8-naphthalic anhydride (NA) prevented32P leakage from onion roots in the presence of 1 × 10−5M metolachlor. High concentrations of NA [0.1% (w/v) suspensions], however, promoted32P leakage and did not protect onion roots from the leakage induced by high concentrations (1 × 10−4M) of metolachlor. Neither metolachlor nor alachlor [2-chloro-2′,6′-diethyl-N-(methoxymethyl)acetanilide], at 1 × 10−4M, inhibited the uptake of acetate-2-14C or malonic acid-2-14C into excised cotton root tips or the incorporation of the precursors into lipids. Similarly, neither herbicide inhibited phospholipid synthesis by cotton root tips. Incorporation of14C-choline chloride into phosphatidylcholine was not significantly inhibited by metolachlor.

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