Protective Effect of Resveratrol on Hepatocyte Apoptosis in Alcoholic Liver

2021 ◽  
Vol 15 (3) ◽  
pp. 385-391
Author(s):  
Yu-Meng Liu ◽  
Ying Liu ◽  
Bo-Xin Dou ◽  
Madhujith Terrence
Keyword(s):  
Protein Expression ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Control Group ◽  
Lipid Peroxidation Product ◽  
Model Control ◽  
Peroxidation Product ◽  
Continuous Feeding ◽  
Glutamyl Transferase

Resveratrol is a phytoalexin produced by several plants. To investigate its mechanism on prevention of alcoholic liver injury, 72 male rats with alcoholic liver disease were randomly divided into 6 groups (blank, model, positive drug, high, medium and low dose of resveratrol groups). After 30 days of continuous feeding, the levels of various indexes were detected; TUNEL assay was used to detect the apoptosis of liver cells; the expression of CYP2E1, SIRT-1, NF-κB and TNF-α was detected by western blot. In the results, the activities of Alanine transaminase (ALT), Aspartate aminotransferase (AST), lactate dehydrogenase (LDH), low-density lipoprotein cholesterol (LDL-C), total bilirubin (TBIL) and y-glutamyl transferase (GGT) in serum of resveratrol groups were significantly lower than those of model control group, the activity of glutathione (GSH) and superoxide dismutase (SOD) in high and medium dose resveratrol group was significantly increased. The contents of Lipid peroxidation product malondialdehyde (MDA) and Reactive Oxygen Species (ROS) in the groups with high, medium and low doses of resveratrol were significantly reduced. Resveratrol could significantly reduce the protein expression of CYP2E1, NF-κB and TNF-α in rat liver tissue; the protein expression of SIRT-1 was significantly up-regulated. In conclusion, resveratrol has a remarkable antioxidant effect.

Effect of Turmeric and Ginger on Lipid Profile in Male Rats Exposed to Oxidative Stress

2019 ◽  
Vol 10 (01) ◽  
Author(s):  
Eman A. Al-Rekabi ◽  
Dheyaa K. Alomer ◽  
Rana Talib Al-Muswie ◽  
Khalid G. Al-Fartosi
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Drinking Water ◽  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Control Group ◽  
Very Low Density Lipoprotein ◽  
Low Density

The present study aimed to investigate the effect of turmeric and ginger on lipid profile of male rats exposed to oxidative stress induced by hydrogen peroxide H2O2 at a concentration of 1% given with consumed drinking water to male rats. Methods: 200 mg/kg from turmeric and ginger were used, and the animals were treatment for 30 days. Results: the results showed a significant increase in cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), whereas it explained a significant decrease in high density lipoprotein (HDL) of male rats exposed to oxidative stress when compared with control group. the results showed a significant decrease in cholesterol, triglycerides, (LDL), (VLDL), whereas it explained a significant increase in (HDL) of rats treated with turmeric and ginger at dose 200 mg/kg when compared with male rats exposed to oxidative stress.

scholarly journals Interaction of 4-hydroxynonenal-modified low-density lipoproteins with the fibroblast apolipoprotein B/E receptor

1986 ◽  
Vol 234 (1) ◽  
pp. 245-248 ◽  
Author(s):  
W Jessup ◽  
G Jurgens ◽  
J Lang ◽  
H Esterbauer ◽  
R T Dean
Keyword(s):  
Apolipoprotein B ◽  
Negative Charge ◽  
Low Density Lipoprotein ◽  
Ldl Receptor ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipid Peroxidation Product ◽  
Modified Low Density Lipoproteins ◽  
Peroxidation Product

The incorporation of the lipid peroxidation product 4-hydroxynonenal into low-density lipoprotein (LDL) increases the negative charge of the particle, and decreases its affinity for the fibroblast LDL receptor. It is suggested that this modification may occur in vivo, and might promote atherogenesis.

scholarly journals Effect of Hyper and Hypothyroidism on Lipid Profile and Liver Function of Male Rats

2011 ◽  
Vol 8 (4) ◽  
pp. 926-933
Author(s):  
Baghdad Science Journal
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Physiological Saline ◽  
Serum Levels ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Control Group ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Significant Difference

This study was designed to investigate the effect of thyroid hormone disturbance on lipids profiles and liver functions. Eighteen mature male rats Rattus norvegicus were divided into three groups. The first and the second groups were injected subcutaneously with thyroxine (T4) and carbimazol (both at 600 µg/kg BW) respectively on alternate days, to produce recurrent periods of hyper and hypothyroidism .The control group which is the third group was injected with physiological saline. The process continued 4 weeks, after that, injection, blood specimens were collected to estimate serum levels of T3 and T4, Total cholesterol (TC), Triglycerides (TG), High density lipoprotein cholesterol (HDL-C), Low density lipoprotein cholesterol (LDL-C) and Very low density lipoprotein cholesterol (VLDL-C) were determined in the serum. In addition to that, we measure GOT, GPT and CPK enzymes activity. The results showed no significant difference in body weight and a significant increase (P

scholarly journals Protective Effect of Vitamins C and E against Gasoline Vapors Induced Haematological and Biochemical Changes in Male Rats

2015 ◽  
Vol 7 (3) ◽  
pp. 139-149
Author(s):  
A. Sedky ◽  
H. Elsawy
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hemoglobin Concentration ◽  
Protective Role ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Total Serum Protein ◽  
Total Serum ◽  
Control Group ◽  
Concomitant Treatment

The present work was designed to investigate the changes in some hematological, biochemical parameters and lipid profile as well as possible protective role of vitamins C and E against gasoline vapors induced toxicity in male rats. The present results showed that gasoline 80 vapors significantly decreased (p?0.05) the concentration of total serum protein (TSP) and albumin concentrations and increased (p?0.05) in serum activities of serum aminotransferases (ALT and AST) and alkaline phosphatase (ALP) compared to the control group. Also, exposure to gasoline 80 vapors induced significant decrease (p?0.05) in hemoglobin concentration, red blood cell count (RBCs), packed cell volume (PCV) and high density lipoprotein cholesterol (HDL-C) compared to control group. On the other hand, exposure to gasoline 80 vapors resulted in significant increase (p?0.05) in the levels of total cholesterol (TC), triglycerides (TGs), LDL-cholesterol (LDL-C) and very low density lipoprotein cholesterol (VLDL-C) relative to untreated experimental animals. However, concomitant treatment with gasoline vapors and administration of vitamins C and E exhibited a protective role on the observed toxic effect of gasoline vapors in male rats. The results of the present study indicated that toxic effects of gasoline vapors could be reduced by dietary supplementation of vitamins C and E.

scholarly journals PREPARATION OF SALVIA OFFICINALIS L. EXTRACT AND STUDY OF ITS EFFECT ON SOME PHYSIOLOGICAL VARIABLES IN ALBINO RATS

2021 ◽  
Vol 12 (01) ◽  
Author(s):  
Wijdan I.A. Abd- Alwahab ◽  
Wijdan I.A. Abd- Alwahab ◽  
Wijdan I.A. Abd- Alwahab
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Salvia Officinalis ◽  
Male Rats ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Low Density ◽  
Physiological Variables ◽  
White Rats ◽  
Salvia Officinalis L

Salvia officinalis is one of the most valued herbs because of its high amount of essential oil and its many components. It has many health-related uses such as improving cognition, as well as reducing the amount of nursing mother's milk during weaning, also recommended for the gargling of the infectious throat, and to treat cardiovascular diseases and nervous disturbance, depression, cerebral ischemia and acts as an antiseptic for wounds. This study aim was to prepare the plant extract of Salvia officinalis L. from the Iraq local areas, determine its chemical properties and study its effect on some physiological and immunological variables in white rats. The plant leave Extract was Prepared and its effects were assessed on several physiological parameters using 20 male rats. The rats were divided into four groups. the control group(G1) received standard food and water; the negative control group (G2) received fat rich diet; G3 rats were given Salvia officinalis with a concentration of 100 mg\ kg\day. G4: rats were given Salvia officinalis 100mg/kg body weight and received fat rich diet. Roche/Hitachi, cobas c 501/502 were used to evaluate the levels of aminotransferase Enzymes, Aspartate transaminase (AST), Alanine aminotransferase (ALT), alkaline phosphatase enzyme (ALP), serum creatinine, serum urea, total protein, albumin, cholesterol, triglyceride, and high-density lipoprotein (HDL). While Globulin concentration, very Low-Density Lipoprotein (VLDL), and Low-Density Lipoprotein (LDL) were calculated according to some referred equations. The phytochemical analysis showed that the compounds identified in the sample were found to be alkaloids, phenols, tannins, coumarins glycosides, flavonoids, quinines, Carbohydrates and steroids. The results showed a significant increase (P <0.01) in the level of liver enzymes ALT, AST and ALP, in the G2 treated with the high fat diet compared with a control group and showed a significant increase (P <0.01) in the level of urea an

Study on the Hypolipidemic Effect and Antioxidant Activity of Seabuckthorn Marc Flavonoids

2011 ◽  
Vol 345 ◽  
pp. 292-296 ◽  
Author(s):  
Yan Jiao ◽  
Xi Qun Zheng ◽  
Xiao Lan Liu ◽  
Ying Chang ◽  
Zhen Yu Wang
Keyword(s):  
Antioxidant Activity ◽  
Hepatic Lipase ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
The Body ◽  
Lipoprotein Cholesterol ◽  
Control Group ◽  
Hypolipidemic Effect ◽  
Model Control ◽  
Model Control Group

The hypolipidemic effect and anti-oxidative activity of seabuckthorn marc flavonoids (SMF) on hyperlipidemic rats was investigated. The rats were divided into 5 groups, including normal control group, hyperlipidemic model control group and 3 different doses of SMF groups i.e. 3.0 mg/kg·bw, 30.0 mg/kg·bw, 90mg/kg·bw, respectively. The SMF was given orally for 35 days. After the treatment, the body, fat and liver weights of rats were weighed. The content of serum total triglycerides (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and the activity of lipid metabolism and anti-oxidative enzyme were determined. The levels of serum lipids in hyperlipidemic rats fed with SMF were significantly lower than model control group. The activities of hepatic lipase (HL), lipoprotein lipase (LPL), lipidase (LPS), superoxide dismutase (SOD), glutathione (GSH) in liver were increased, while the contents of malondialdehyde (MDA) were decreased in SMF groups. The results indicate that SMF has significantly hypolipidemic effects and antioxidant activity.

Modulation of lead biohazards using a combination of epicatechin and lycopene in rats

2011 ◽  
Vol 30 (10) ◽  
pp. 1674-1681 ◽  
Author(s):  
Taha A Komousani ◽  
Said S Moselhy
Keyword(s):  
Low Density Lipoprotein ◽  
Combined Treatment ◽  
Density Lipoprotein ◽  
Blood Lead ◽  
The Other ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Individual Treatment ◽  
Control Group ◽  
Sod Activity

The toxicity of many heavy metals is due to their ability to cause oxidative damage to tissues. Lead is one of the most important metals that pollute the natural environment due to man’s impact The aim of this study is to investigate the potential protective effect of epicatechin alone or combined with lycopene against toxicity of lead in male rats. Five groups of rats were involved in this study; the first was control while the other four injected with lead acetate (100 mg/kg BW) subcutaneous for 2 weeks. On the other hand, the third, fourth and fifth groups were injected with epicatechin, lycopene or epicatechin + lycopene, respectively. Results obtained showed that, the combined treatment (epicatechin + lycopene) exert its effects (100%) against toxic effects against lead by lowering the liver enzymes alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and gamma glutamyle transferase (GGT) activities and decrease lipid peroixdation (MDA) and enhances the superoxide dismutase (SOD) activity. The high-density lipoprotein cholesterol (HDL-c) level was significantly decreased and low-density lipoprotein cholesterol (LDL-c) level was statistically significantly increased in lead-injected rats as compared with control group. The combined treatment with epicatechin and lycopene justify these levels to nearly normal values. The erythrocyte level of total glutathione was decreased in lead-injected rats as compared with control group ( p < 0.001). The combined effect is significantly higher than individual treatment lycopene alone or epicatechin. A negative correlation was found between the blood lead and SOD (r = −0.6) and glutathione (r = −0.81) while a positive correlation with MDA level (r = 0.7).

scholarly journals Comparison between the Hypolipidemic Activity of Parsley and Carob in Hypercholesterolemic Male Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Haddad A. El Rabey ◽  
Madeha N. Al-Seeni ◽  
Habibah B. Al-Ghamdi
Keyword(s):  
Methanol Extract ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Albino Rats ◽  
Low Density ◽  
Creatine Kinase Mb ◽  
Liver Tissues

Hypercholesterolemia is commonly associated with obesity that leads to heart diseases and diabetes. The hepatocardioprotective activity of parsley and carob methanol extract was tested in hypercholesterolemic male rats. Twenty-four male albino rats were divided into four groups (n=6). Group 1 was the negative control group fed with fat rich diet, group 2 (G2) was hypercholesterolemic rats fed with fat rich diet with 2% cholesterol, and group 3 and group 4 (G3 and G4) were hypercholesterolemic rats supplemented with 2% cholesterol and cotreated with 20% w/w parsley seed methanol extract and 20% w/w carob legume methanol extract, respectively. The experiment was conducted for eight weeks. The positive hypercholesterolemic rats showed significant increase in serum levels of total cholesterol, triglycerides, low density lipoprotein (LDL), very low density lipoprotein (VLDL), lactate dehydrogenase (LDH), creatine kinase-mb, liver function enzymes, and decrease in the high density lipoproteins (HDL). Moreover, heart and liver tissues were ameliorated and nearly restored their normal appearance. It could be concluded that both parsley and carob extracts supplementations have a protective effect against hyperlipidemia and improved the histological alteration in heart and liver tissues. The methanol extract of parsley appeared to be more efficient than that of carob in lowering hypercholesterolemia.

scholarly journals Effect of Tannin-Rich Extract of Chasmanthera dependens on Piroxicam-induced Liver Damage in Male Wistar Rats

2021 ◽  
Vol 5 (1) ◽  
pp. 27
Author(s):  
Tijani Stephanie Abiola ◽  
Olori Ogaraya David ◽  
Farombi Ebenezer Olatunde
Keyword(s):  
Oxidative Stress ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Significant Rise ◽  
Control Group ◽  
Gamma Glutamyl Transferase ◽  
Male Wistar Rats ◽  
Anti Inflammatory ◽  
Group 2 ◽  
Glutamyl Transferase

Background: Piroxicam is one of the nonsteroidal anti-inflammatory drugs used as antipyretic, analgesic and anti-inflammatory drug often used for the relief of nonspecific fever condition and in arthritis. This study investigated the protective potential of tannin-rich extract of Chasmanthera dependens (TRECDS) against piroxicam-induced hepatotoxicity in male Wistar rats.Materials and Methods: Thirty two rats were divided into four groups. Group 1 received normal saline and served as the control group, group 2 were given 20 mg/kg piroxicam only, while groups 3 and 4 were given 20 mg/kg piroxicam with the addition of 200 and 400 mg/kg of tannin-rich extract of Chasmanthera dependens, respectively. All rats were treated orally once daily for ten days.Results: Administration of piroxicam caused liver atrophy demonstrated by significant rise in serum alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), glucose-6-phosphate dehydrogenase (G6PDH) levels of albumin (ALB), bilirubin (BIL), total cholesterol (TCHOL), triglyceride (TRIGS) and low-density lipoprotein (LDL). Piroxicam also decreased high-density lipoprotein (HDL) level, enzymatic and nonenzymatic antioxidant levels significantly (p>0.05) with attendant increase in oxidative stress indices in the liver of rats compared with control group. Histological assessment reveled severe damaged to the liver of rats. However, co-administration with TRECDS reversed these observations as evidenced in the histological results.Conclusion: The findings of this study showed that exposure of rats to piroxicam provoked damage to the liver via oxidative damage and TRECDS has the potential of ameliorating the damage.Keywords: hepatotoxicity, piroxicam, Chasmanthera dependens, oxidative stress

Sign in / Sign up

Export Citation Format

Share Document