Efficient antibacterial polyphosphazene material with potential to prominent wound healing

2021 ◽  
Vol 11 (6) ◽  
pp. 947-958
Author(s):  
Sitao Shen ◽  
Zhenying Guo ◽  
Jiahao Wang ◽  
Feng Chen ◽  
Di Wu ◽  
...  
Keyword(s):  
Wound Dressings ◽  
Excellent Thermal Stability ◽  
E Coli ◽  
Structures And Properties ◽  
Confocal Immunofluorescence ◽  
Bactericidal Effects ◽  
Bacterial Growth Curve ◽  
Low Cytotoxicity ◽  
Long Acting ◽  
Simple Combination

Reducing the toxicity of silver-based antibacterial agents used in gels and improving their biocompatibility are of great significance in the development of wound dressings. Thus, a highly crosslinked organic–inorganic hybrid porous polyphosphazene nanospheres (PRV-HMSs) were prepared via precipitation polymerization. The structures and properties were investigated. The results indicated that PRV-HMSs have uniform particle size about 500 nm, excellent thermal stability, high silver loading (26.5 wt.%) and remarkable sustained-release properties after loading AgNO3 (up to 2 weeks). The antibacterial effects were investigated with zone of inhibition testing, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), Live/Dead bacterial staining assay and bacterial growth curve assays. The silver-loaded nanospheres have excellent bactericidal effects for E. coli or S. aureus and an average bactericidal rate of 94.02% and 94.67% in the 120 hour long-acting antibacterial test. The cytotoxicity and laser confocal immunofluorescence staining experiments indicate that the simple combination of nanospheres and agar not only give low cytotoxicity, but also significantly promote the activation of macrophages, which showing potential application in the wound dressings based on hydrogel.

scholarly journals Antibacterial Potentials of some Toothpaste Products against some Oral Bacterial Isolates

2021 ◽  
Vol 24 (2) ◽  
Author(s):  
Bernard Oluwapelumi Oluboyo ◽  
Maihankali J Charles ◽  
Richard Akele ◽  
Funmilayo Akinseye ◽  
Adeola Oluboyo
Keyword(s):  
Benzyl Alcohol ◽  
Sodium Fluoride ◽  
Zinc Sulphate ◽  
Potassium Nitrate ◽  
Inhibitory Effect ◽  
Oral Microbiome ◽  
Diffusion Method ◽  
E Coli ◽  
Test Microorganism ◽  
Bactericidal Effects

Objetive: Manufacturers of toothpastes claim that their products are active against oral microbiome capable of causing tooth decay. The objective of this study was to investigate the manufacturers’ claim using some of the toothpaste products sold in Ado-Ekiti, Nigeria. Material and methods: The antibacterial potentials of five commercialized toothpaste products (designated sodium fluoride-zinc sulphate, benzyl alcohol-sodium fluorophosphate, sodium fluoride-eugenol, sodium fluoridesodium laurylsulfate and sodium fluoridepotassium nitrate) were tested against six oral isolates of dental caries and periodontal origin – Staphylococcus aureus, Streptococcus mitis, Streptococcus salivarius, Streptococcus pyogenes and Pseudomonas aeruginosa. The antimicrobial potentials were evaluated using modified agar well diffusion method. Various dilutions of the toothpaste products from 1:1 to 1:16 were tested against each test microorganism. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the toothpastes were determined. Results: sodium fluoride-zinc sulphate, benzyl alcoholsodium fluorophosphate and sodium fluorideeugenol toothpastes showed inhibitory effects on S. aureus, S. mitis and S. salivarius. Sodium fluoride-sodium laurylsulfate and sodium fluoride-potassium nitrate toothpastes showed no inhibitory effect on the organisms except S. pyogenes. Only sodium fluoride-potassium nitrate toothpaste inhibited E. coli while none of the toothpastes inhibited P. aeruginosa. The MIC and MBC of sodium fluoride-zinc sulphate, benzyl alcohol-sodium fluorophosphate, and sodium fluoride-eugenol toothpastes showed bacteriostatic and bactericidal effects on the organisms. Sodium fluoride-zinc sulphate, benzyl alcohol-sodium fluorophosphate, and sodium fluoride-eugenol toothpastes showed comparable effects on S. aureus, S. mitis and S. salivarius. Sodium fluoride-eugenol toothpaste was strongest against S. mitis, benzyl alcoholsodium fluorophosphates toothpaste was strongest against S. pyogenes, sodium fluoridezinc sulphate toothpaste was strongest against S. salivarius and only sodium fluoride-potassium nitrate toothpaste inhibited E. coli. Conclusion: The manufacturer’s claim is upheld by this study for sodium fluoride-zinc sulphate, benzyl alcohol-sodium fluorophosphate and sodium fluoride-eugenol toothpastes. However, sodium fluoride-sodium laurylsulfate and sodium fluoride-potassium nitrate toothpastes showed limited inhibitory potentials.     Keywords Antibacterial; Caries; Oral isolates; Periodontitis; Toothpastes.

A Synergistic Antimicrobial Mechanism of GO: Why Oxidative Stress Can Inactivate E. coli

NANO ◽  
2020 ◽  
Vol 15 (04) ◽  
pp. 2050054 ◽  
Author(s):  
Xin Li ◽  
Haoqi Zhao ◽  
Shidong Wang ◽  
Weiwu Zou ◽  
Peiyan Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antibacterial Activity ◽  
Mammalian Cells ◽  
Water Solubility ◽  
Direct Interaction ◽  
Medical Application ◽  
Future Application ◽  
Sem Images ◽  
E Coli ◽  
Low Cytotoxicity

Graphene oxide (GO), a 2D nanomaterial, is a promising material for medical application, thanks to its water solubility, antibacterial activity and relatively low cytotoxicity. However, many factors, such as lateral dimension, purity and surface chemistry, may influence its antibacterial activity, its exact mechanism is still unknown. In this work, E. coli was used as model bacterium to determine the antibacterial activity of well-dispersed GO which was obtained by a modified Hummer method and dialyzed to remove the salts and acid used in the oxidation process. After co-culture with GO for 2[Formula: see text]h, up to 90% E. coli cells were inactivated when GO concentration at 8[Formula: see text][Formula: see text]g/mL. The direct interaction was not detected in FE-SEM images and the results of [Formula: see text] potential showed that the interaction between GO and E. coli are repulsive[Formula: see text] Our results showed that GO can produce ROS and inactivate SOD and CAT enzymes in low concentration after co-cultured with E. coli which explained the antibacterial activity of GO in aqueous solution. Meanwhile, GO, with high purity, showed low cytotoxicity towards mammalian cells and did not cause any observable hemoglobin after co-cultured with blood cells. The data presented here prove that GO is effectively inhibit E. coli through inactivating SOD, CAT enzymes and the oxidative stress produced by ROS. Furthermore, the good biocompatibility promised its future application.

scholarly journals Certain Aspects of Silver and Silver Nanoparticles in Wound Care: A Minireview

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Marek Konop ◽  
Tatsiana Damps ◽  
Aleksandra Misicka ◽  
Lidia Rudnicka
Keyword(s):  
Silver Nanoparticles ◽  
Clinical Practice ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Antifungal Agents ◽  
Wound Care ◽  
Wound Dressings ◽  
Major Health Problem ◽  
Major Health ◽  
Bactericidal Effects

Resistance to antimicrobial agents by pathogenic bacteria has emerged in recent years and is a major health problem. In this context silver and silver nanoparticles (AgNP) have been known to have inhibitory and bactericidal effects and was used throughout history for treatment of skin ulcer, bone fracture, and supporting wound healing. In all of these applications prevention and treatment of bacterial colonized/infected wounds are critical. In this context silver and its derivatives play an important role in health care. Silver is widely used in clinical practice in the form of silver nitrate and/or silver sulfadiazine. In the last few years silver nanoparticles entered into clinical practice as both antimicrobial and antifungal agents. In addition, nanosilver is used in coating medical devices (catheters) and as component of wound dressings. In this paper we present summarized information about silver and nanoparticles made of silver in the context of their useful properties, especially antibacterial ones, being of a great interest for researchers and clinicians.

scholarly journals Synthesis, Characterization, and Antimicrobial Evaluation of Random Poly(ester-Carbonate)s Bearing Pendant Primary Amine in the Main Chain

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2640
Author(s):  
Peng Dong ◽  
Jing Feng ◽  
Sujuan Li ◽  
Tingli Sun ◽  
Qingshan Shi ◽  
...  
Keyword(s):  
Primary Amine ◽  
Ring Opening ◽  
Inhibitory Concentration ◽  
Cyclic Carbonate ◽  
Random Copolymers ◽  
Feed Ratio ◽  
E Coli ◽  
Antimicrobial Evaluation ◽  
Low Cytotoxicity ◽  
Poly Caprolactone

Starting from primary amine bearing cyclic carbonate tert-butyl-(2-oxo-1,3-dioxan-5-yl) carbamate (TBODC) and caprolactone (CL), amphiphilic poly(caprolactone-ran-amino trimethyl carbonate)s (P(CL-ran-ATC)s) random copolymers with controlled molecular weight and composition were synthesized via ring opening polymerization (ROP) and deprotection, using stannous octoate (Sn(Oct)2) as catalyst and benzyl alcohol (BnOH) as initiator. Therefore, hydrophilic/lipophilic ratio (HLR) of the P(CL-ran-ATC)s copolymers can be finely adjusted by the feed ratio of TBODC and CL. The antimicrobial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) of P(CL-ran-ATC)s were proportional to HLR, and P(CL-ran-ATC)s presented more vigorous bactericidal activity towards S. aureus. The minimum inhibitory concentration (MIC) values of P(CL-ran-ATC 50.9%) are 2000 μg mL−1 and 3000 μg mL−1 for S. aureus and E. coli. While P(CL-ran-ATC 50.9%) exhibited deficient hemolytic activity as 1.41%. In addition, the P(CL-ran-ATC)s showed extremely low cytotoxicity towards fibroblast L929 cells.

scholarly journals Chitosan & Conductive PANI/Chitosan Composite Nanofibers - Evaluation of Antibacterial Properties

2019 ◽  
Vol 4 (1) ◽  
pp. 6-20 ◽  
Author(s):  
Panagiota Moutsatsou ◽  
Karen Coopman ◽  
Stella Georgiadou
Keyword(s):  
Chronic Wounds ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Bacterial Strains ◽  
Healthcare Applications ◽  
E Coli ◽  
Jet Stability ◽  
Electrospun Membranes ◽  
New Treatments ◽  
Chitosan Composite

Background: Within the healthcare industry, including the care of chronic wounds, the challenge of antimicrobial resistance continues to grow. As such, there is a need to develop new treatments that can reduce the bioburden in wounds. Objective: The present study is focused on the development of polyaniline (PANI) / chitosan (CH) nanofibrous electrospun membranes and evaluates their antibacterial properties. Methods: To this end, experimental design was used to determine the electrospinning windows of both pure chitosan and PANI/CH blends of different ratios (1:3, 3:5, 1:1). The effect of key environmental and process parameters (relative humidity and applied voltage) was determined, as well as the effect of the PANI/CH ratio in the blend and the molecular interactions between PANI and chitosan that led to jet stability. Results: The nanofibrous mats were evaluated regarding their morphology and antibacterial effect against model gram positive and gram negative bacterial strains, namely B. subtilis and E. coli. High PANI content mats show increased bactericidal activity against both bacterial strains. Conclusion: The blend fibre membranes combine the materials’ respective properties, namely electrical conductivity, biocompatibility and antibacterial activity. This study suggests that electrospun PANI/CH membranes are promising candidates for healthcare applications, such as wound dressings.

scholarly journals Toxoplasma gondii GRA9 Regulates the Activation of NLRP3 Inflammasome to Exert Anti-Septic Effects in Mice

2020 ◽  
Vol 21 (22) ◽  
pp. 8437
Author(s):  
Jae-Sung Kim ◽  
Seok-Jun Mun ◽  
Euni Cho ◽  
Donggyu Kim ◽  
Wooic Son ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Nlrp3 Inflammasome ◽  
Dense Granule ◽  
E Coli ◽  
Pyrin Domain ◽  
Essential Components ◽  
Bactericidal Effects ◽  
Anti Inflammatory

Dense granule proteins (GRAs) are essential components in Toxoplasma gondii, which are suggested to be promising serodiagnostic markers in toxoplasmosis. In this study, we investigated the function of GRA9 in host response and the associated regulatory mechanism, which were unknown. We found that GRA9 interacts with NLR family pyrin domain containing 3 (NLRP3) involved in inflammation by forming the NLRP3 inflammasome. The C-terminal of GRA9 (GRA9C) is essential for GRA9–NLRP3 interaction by disrupting the NLRP3 inflammasome through blocking the binding of apoptotic speck-containing (ASC)-NLRP3. Notably, Q200 of GRA9C is essential for the interaction of NLRP3 and blocking the conjugation of ASC. Recombinant GRA9C (rGRA9C) showed an anti-inflammatory effect and the elimination of bacteria by converting M1 to M2 macrophages. In vivo, rGRA9C increased the anti-inflammatory and bactericidal effects and subsequent anti-septic activity in CLP- and E. coli- or P. aeruginosa-induced sepsis model mice by increasing M2 polarization. Taken together, our findings defined a role of T. gondii GRA9 associated with NLRP3 in host macrophages, suggesting its potential as a new candidate therapeutic agent for sepsis.

Silver and hydrogen peroxide as potential drinking water disinfectants: their bactericidal effects and possible modes of action

1997 ◽  
Vol 35 (11-12) ◽  
pp. 87-93 ◽  
Author(s):  
R. Pedahzur ◽  
H. I. Shuval ◽  
S. Ulitzur
Keyword(s):  
Hydrogen Peroxide ◽  
Synergistic Effect ◽  
Vibrio Fischeri ◽  
E Coli ◽  
Bactericidal Effects ◽  
Synergistic Induction ◽  
Response Systems ◽  
K 12 ◽  
Consequent Increase

Silver and hydrogen peroxide (HP) acted synergistically on the viability of E. coli K-12. In certain concentration ranges the synergistic effect amounted to about 3 logs. Toxicity process kinetics were determined by following the decrease in luminescence of a highly luminescent recombinant E. coli harbouring a plasmid carrying the whole lux system of Vibrio fischeri. As in the viability studies, silver and HP also showed a synergistic effect on in vivo luminescence, which amounted to a 2 log increase in toxicity. A similar phenomenon was found for silver and certain metal ions, including Cu2+, Ni2+, Zn2+ and Cd2+, where toxicity increased by a factor of 10 in the presence of sub-inhibitory concentrations of silver. To monitor the expression of different stress response systems in treated cells, we have used E. coli carrying fusions of the lux system to promoters of different stress-controlling genes. Of the fusions tested, HP substantially increased the activity of recA, katG, micF, grpE, lon, and dnaK. Silver exerted a mild effect acting only on grpE and lon promoters. When in combination, a synergistic induction of the dnaK fusion and a slightly additive effect on grpE fusion were recorded. It appears that the combined toxic effect of silver and HP may be related with damage to cellular proteins. Nevertheless, the involvement of other cellular moieties can not be ruled out. The possibility that the synergistic effect is related to chemical interactions between silver and HP and the consequent increase in their toxicity is discussed.

scholarly journals Soluble insulin analogs combining rapid- and long-acting hypoglycemic properties – From an efficient E. coli expression system to a pharmaceutical formulation

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0172600 ◽  
Author(s):  
Diana Mikiewicz ◽  
Anna Bierczyńska-Krzysik ◽  
Agnieszka Sobolewska ◽  
Dorota Stadnik ◽  
Monika Bogiel ◽  
...  
Keyword(s):  
Expression System ◽  
Pharmaceutical Formulation ◽  
Insulin Analogs ◽  
E Coli ◽  
Soluble Insulin ◽  
Long Acting

scholarly journals Comparative Study of Physicochemical and Antibacterial Properties of ZnO Nanoparticles Prepared by Laser Ablation of Zn Target in Water and Air

2018 ◽  
Author(s):  
Ekaterina A. Gavrilenko ◽  
Daria A. Goncharova ◽  
Ivan N. Lapin ◽  
Anna L. Nemoikina ◽  
Valery A. Svetlichnyi ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Zno Nanoparticles ◽  
Biomedical Applications ◽  
Antibacterial Properties ◽  
Pulsed Laser ◽  
Wound Dressings ◽  
E Coli ◽  
Nanosecond Pulsed Laser ◽  
Bactericidal Properties ◽  
Biodegradable Poly

Here, we report on ZnO nanoparticles (NPs) generated by nanosecond pulsed laser (Nd:YAG, 1064 nm) through ablation of metallic Zn target in water and air and their comparative analysis as potential nanomaterials for biomedical applications. The prepared nanomaterials were carefully characterized in terms of their structure, composition, morphology and defects. It was found that in addition to the main wurtzite ZnO phase, which is conventionally prepared and reported by others, the sample laser-generated in air also contained some amount of monoclinic zinc hydroxynitrate. Both nanomaterials were then used to modify model wound dressings based on biodegradable poly-L-lactic acid. The as-prepared model dressings were tested as biomedical materials with bactericidal properties towards S. aureus and E. coli strains. The advantages of the NPs prepared in air over their counterparts generated in water found in this work are discussed.   

scholarly journals Cefuroxime-Loaded Hydrogels for Prevention and Treatment of Bacterial Contamination of Open Wounds

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Pavlo Virych ◽  
Oksana Nadtoka ◽  
Volodymyr Doroschuk ◽  
Sergey Lelyushok ◽  
Vasyl Chumachenko ◽  
...  
Keyword(s):  
Wound Dressings ◽  
E Coli ◽  
Polyacrylamide Hydrogels ◽  
Wild Strains ◽  
Cross Links ◽  
Antibiotic Release ◽  
Synthesized Materials ◽  
Klebsiella Spp

Dextran/Sulfodextran-graft-polyacrylamide- and polyacrylamide-based hydrogels were synthesized by radical polymerization and loaded with cefuroxime to obtain antimicrobial wound dressings. Antibiotic release from the antibiotic-loaded hydrogels into an aqueous solution was studied by the HPLC-UV method. It is shown that cefuroxime-loaded Dextran/Sulfodextran-graft-polyacrylamide hydrogels release the antibiotic more slowly compared to polyacrylamide hydrogel with the same density of cross-links. Antibacterial activity of the synthesized materials was tested in vitro against wild strains of S. aureus, E. coli, and Klebsiella spp. The possibility of using the obtained antimicrobial hydrogels for the treatment of infected wounds was confirmed in vivo in a rat model.

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