Immunoglobulins and Blood Vessels in the Tonsillar Crypt Epithelium

1973 ◽  
Vol 82 (3) ◽  
pp. 359-369 ◽  
Author(s):  
John F. Schmedtje ◽  
Ann F. Batts
Keyword(s):  
Epithelial Cells ◽  
Blood Vessels ◽  
Plasma Cells ◽  
Border Zone ◽  
Secretory Iga ◽  
Antigenic Determinants ◽  
Crypt Epithelium ◽  
Lower Border

The localization of IgA, IgG, IgM, SP and the relationships of plasma cells and lymphocytes to blood vessels in the tonsillar crypt epithelium were investigated. Immunofluorescent techniques were used that included antisera specific for the two antigenic determinants of external secretory IgA, namely, 4s SP and 7s IgA, and also antisera specific for 7s IgG and 19s IgM. The secretory piece was absent in the crypt epithelium and in most of the crypt lumen. Aggregations of plasmacyte series cells, containing either IgG, IgA, or IgM were present in the crypt epithelium. Mature plasma cells of these aggregations abutted against the walls of blood sinusoids located in the epithelium, which suggested secretion into these sinusoids. All three immunoglobulins were also identified between epithelial cells and small lymphocytes. Postcapillary venules with emigrating small lymphocytes abounded in sub-epithelial sites, and were present at the lower border zone of the epithelium. Lymphocytes in shapes of diapedesis were observed in the endothelium of epithelial blood sinusoids. These observations are in accord with the hypothesis that a “circulation” of many lymphocytes occurs in the epithelium facilitating the activation of any one genetically committed lymphocyte.

LOCAL IMMUNOLOGICAL RESPONSE IN THE VAGINA, CERVIX AND ENDOMETRIUM

1975 ◽  
Vol 80 (2_Suppl) ◽  
pp. S281-S305 ◽  
Author(s):  
J.-P. Vaerman ◽  
J. Férin
Keyword(s):  
Epithelial Cells ◽  
Genital Tract ◽  
Plasma Cells ◽  
Specific Binding ◽  
Female Genital Tract ◽  
Secretory Component ◽  
Secretory Iga ◽  
Local Synthesis ◽  
Female Genital

ABSTRACT The mechanism of the local excretion of secretory IgA (SIgA) in exocrine secretions has been reviewed. Numerous local IgA-plasma cells, in the lamina propria of the glandular mucosa, synthesize dimeric IgA with J-chain. Free secretory component (FSC) is synthesized and accumulated in the Golgi area of the columnar epithelial cells. It is then supposed to get onto their cell membranes. Dimeric IgA (and some IgM) reaches the spaces between and under the epithelial cells. There, a specific binding occurs between dimeric IgA (and some IgM) and the FSC located in the cell-membrane, whereby SIgA is formed. The complex becomes mobilized and is transported toward the apical part of the cell, where it will be excreted into the mucous coat covering the epithelium. In the female genital tract, the cervical mucosa appears to be better adapted to achieve a local secretory immune system. The endometrium seems less suitable, being normally short of local plasma cells. The vaginal wall appears almost incompatible with the proposed mechanism of local antibody secretion. Criteria for establishing a local immune response in the female genital tract comprise: 1) a lack of correlation between antibody titers in secretions and serum; 2) the demonstration that the secretory antibodies are mainly of IgA class and 3) that they are SIgA molecules, possessing bound secretory component. However, the best criterion would be 4) the observation that antibody is actually synthesized in samples of mucosa, by in vitro culture or immunohistology. Reviewing the literature, relatively few examples were found where SIgA antibodies were demonstrated, and unambiguous evidence for their local synthesis is almost non-existent. In addition, the authors were unable to detect antibody-containing cells in cervical and endometrial biopsies of women locally "immunized" with horse spleen ferritin and bovine serum albumin. The need for further investigation with simple antigens and adequate immunological reagents is stressed.

scholarly journals Correlations between Antibody Immune Responses at Different Mucosal Effector Sites Are Controlled by Antigen Type and Dosage

2000 ◽  
Vol 68 (7) ◽  
pp. 3830-3839 ◽  
Author(s):  
Dörthe Externest ◽  
Barbara Meckelein ◽  
M. Alexander Schmidt ◽  
Andreas Frey
Keyword(s):  
Cholera Toxin ◽  
Plasma Cells ◽  
Immunoglobulin A ◽  
Secretory Iga ◽  
Enzyme Linked Immunosorbent Assay ◽  
Routine Diagnostics ◽  
Significant Relationships ◽  
Serum Igg ◽  
Mucosal Surfaces ◽  
Secretory Immunoglobulin

ABSTRACT Monitoring specific secretory immunoglobulin A (IgA) responses in the intestines after mucosal immunization or infection is impeded by the fact that sampling of small intestinal secretions requires invasive methods not feasible for routine diagnostics. Since IgA plasma cells generated after intragastric immunization are known to populate remote mucosal sites as well, secretory IgA responses at other mucosal surfaces may correlate to those in the intestines and could serve as proxy measures for IgA secretion in the gut. To evaluate the practicability of this approach, mice were immunized intragastrically with 0.2, 2, and 20 mg of ovalbumin plus 10 μg of cholera toxin, and the antigen-specific local secretory IgA responses in duodenal, ileal, jejunal, rectal, and vaginal secretions, saliva, urine, and feces, as well as serum IgG and IgA responses were analyzed by enzyme-linked immunosorbent assay. Correlation analysis revealed significant relationships between serum IgG and IgA, urinary IgA, salivary IgA, and secretory IgA in duodenal, jejunal, ileal, and rectal secretions for the 0.2-mg but not for the 20-mg ovalbumin dose. Fecal samples were poor predictors for intestinal antiovalbumin IgA responses, and no correlations could be established for cholera toxin, neither between local anti-cholera toxin levels nor to the antiovalbumin responses. Thus, specific IgA in serum, saliva, or urine can serve as a predictor of the release of specific IgA at intestinal surfaces after intragastric immunization, but the lack of correlations for high ovalbumin doses and for cholera toxin indicates a strong dependency on antigen type and dosage for these relationships.

scholarly journals  Histological and histochemical studies on the Harderian gland in native chickens

2012 ◽  
Vol 57 (No. 8) ◽  
pp. 404-409 ◽  
Author(s):  
B. Mobini
Keyword(s):  
Epithelial Cells ◽  
Plasma Cells ◽  
Periodic Acid ◽  
Harderian Gland ◽  
Histochemical Staining ◽  
Alveolar Type ◽  
Periodic Acid Schiff ◽  
Parasympathetic Ganglia ◽  
Tissue Sections ◽  
Native Chickens

  The objective of this investigation was to study the histological and histochemical structure of the Harderian gland in native chickens. Samples were obtained from 10 male and 10 female adult healthy native chickens. Tissue sections were stained with haematoxylin eosin, Verhoeff’s, Masson’s trichrome, alcian blue (pH 2.5), periodic acid-Schiff and Gomori’s method for reticulum. The multilobular Harderian gland of native chickens was covered by a thin connective tissue which consisted of adipose tissue, parasympathetic ganglia, nerve bundles, collagen, elastic and reticular fibres. Plasma cells were present in interlobular areas. The Harderian gland was compound tubulo-alveolar type. The Harderian duct was lined by columnar epithelial cells of varying height. Goblet cells were not found in Harderian duct. Histochemical staining revealed that the all epithelial cells of both corpus glandulae and ducts contained both neutral and acidic mucins. No significant sex-based differences were found. It is concluded that the general histological and histochemical structure of the Harderian gland in native chickens is similar to that of domestic geese, but that there are also some differences.  

scholarly journals Comparative studies on monotypic IgM lambda and IgG kappa from an individual patient. IV. Immunofluorescent evidence for a common clonal synthesis

Blood ◽  
1977 ◽  
Vol 50 (2) ◽  
pp. 203-211 ◽  
Author(s):  
JE Hopper
Keyword(s):  
Plasma Cells ◽  
Lymphoproliferative Disorders ◽  
Lymphoid Cells ◽  
Antigenic Determinants ◽  
Sequence Analyses ◽  
Gamma Chain ◽  
Heavy Chains ◽  
Broad Implication ◽  
Clonal Origin ◽  
Immunofluorescent Analysis

Abstract Previous studies have presented evidence of shared idiotypic antigenic determinants located within the variable (VH) region of the heavy chains of monotypic IgMlambda and IgGkappa isolated from the serum of an individual patient, Bro, with Waldenstrom macroglobulinemia. Comparative N-terminal VH sequence analyses have demonstrated that the respective micron and gamma chains belong to separate VH subgroups. The entire VH sequence of the Bro micron chain has been reported, but the VH sequence of the Bro gamma chain still awaits completion. We report the results of an immunofluorescent analysis of cytoplasmic Ig of lymphoid cells isolated from the patient's peripheral blood and bone marrow. Between 6% and 9% of the cytoplasmic Ig-positive lymphoid cells exhibited fluorescent evidence for the dual presence of kappa and lambda chains are well as micron and gamma chains. These results strongly suggest that the idiotypically related Bro IgMlambda and IgGkappa paraproteins are derived from a common clonal origin. Moreover, these findings extend the results of a previous study that has demonstrated the dual presence of IgGkappa and IgGlambda paraproteins within individual myeloma plasma cells. Collectively, these studies suggest that a single neoplastic lymphoid clone may not necessarily be restricted to the synthesis of Ig proteins of the identical light chain class. These findings may have a broad implication for the understanding of surface and cytoplasmic Ig markers of neoplastic lymphoid cells in certain other lymphoproliferative disorders.

Identification, properties, and differential counts of cell populations using electron microscopy of dry cows secretions, colostrum and milk from normal cows

1980 ◽  
Vol 47 (1) ◽  
pp. 39-50 ◽  
Author(s):  
Chee-Seong Lee ◽  
F. B. Peter Wooding ◽  
Patrick Kemp
Keyword(s):  
Epithelial Cells ◽  
Plasma Cells ◽  
Polymorphonuclear Leucocyte ◽  
Polystyrene Latex ◽  
Cell Populations ◽  
Cell Type ◽  
Lactating Cows ◽  
Fresh Milk ◽  
Predominant Cell Type ◽  
Differential Counts

SummaryDifferential counts of electron microscope sections of cell pellets isolated from bovine udder secretions showed that no secretory epithelial cells and very few ductal epithelial cells were present at any stage. The predominant cell type was the macrophage in dry and lactating cows or the polymorphonuclear leucocyte (PMNL) in colostrum. Lymphocytes were also present but no plasma cells were found. The macrophages took up polystyrene latex particles (as did the PMNL) and adhered toglass in culture. Neither macrophage-nor PMNL-rich cell suspensions produced any increase in free fatty acid levels when incubated with fresh milk.

scholarly journals Eotaxin Expression by Epithelial Cells and Plasma Cells in Chronic Asthma

2002 ◽  
Vol 82 (4) ◽  
pp. 495-504 ◽  
Author(s):  
Rakesh K Kumar ◽  
Paul S Thomas ◽  
Da-Qiang Seetoo ◽  
Cristan Herbert ◽  
Andrew N J McKenzie ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Plasma Cells ◽  
Chronic Asthma

scholarly journals Endothelial Claudin

1999 ◽  
Vol 147 (1) ◽  
pp. 185-194 ◽  
Author(s):  
Kazumasa Morita ◽  
Hiroyuki Sasaki ◽  
Mikio Furuse ◽  
Shoichiro Tsukita
Keyword(s):  
Endothelial Cells ◽  
Epithelial Cells ◽  
Blood Vessels ◽  
Polyclonal Antibody ◽  
Polyclonal Antibodies ◽  
Transmembrane Protein ◽  
Velo Cardio Facial Syndrome ◽  
The Brain ◽  
Specific Polyclonal Antibody

Tight junctions (TJs) in endothelial cells are thought to determine vascular permeability. Recently, claudin-1 to -15 were identified as major components of TJ strands. Among these, claudin-5 (also called transmembrane protein deleted in velo-cardio-facial syndrome [TMVCF]) was expressed ubiquitously, even in organs lacking epithelial tissues, suggesting the possible involvement of this claudin species in endothelial TJs. We then obtained a claudin-6–specific polyclonal antibody and a polyclonal antibody that recognized both claudin-5/TMVCF and claudin-6. In the brain and lung, immunofluorescence microscopy with these polyclonal antibodies showed that claudin-5/TMVCF was exclusively concentrated at cell–cell borders of endothelial cells of all segments of blood vessels, but not at those of epithelial cells. Immunoreplica electron microscopy revealed that claudin-5/TMVCF was a component of TJ strands. In contrast, in the kidney, the claudin-5/TMVCF signal was restricted to endothelial cells of arteries, but was undetectable in those of veins and capillaries. In addition, in all other tissues we examined, claudin-5/TMVCF was specifically detected in endothelial cells of some segments of blood vessels, but not in epithelial cells. Furthermore, when claudin-5/TMVCF cDNA was introduced into mouse L fibroblasts, TJ strands were reconstituted that resembled those in endothelial cells in vivo, i.e., the extracellular face–associated TJs. These findings indicated that claudin-5/TMVCF is an endothelial cell–specific component of TJ strands.

Immunologically Induced Changes in the Tonsillar Crypt Epithelium

1979 ◽  
Vol 88 (3) ◽  
pp. 397-406 ◽  
Author(s):  
John F. Schmedtje ◽  
Jose J. Chinea ◽  
Daniel W. Kletzing
Keyword(s):  
Endoplasmic Reticulum ◽  
Epithelial Cells ◽  
Plasma Membranes ◽  
Structural Changes ◽  
Direct Injection ◽  
Lymphatic Tissue ◽  
Microscopic Anatomy ◽  
Crypt Epithelium ◽  
Fine Structures ◽  
Induced Changes

The normal gross and microscopic anatomy of the palatine tonsil of the rabbit was observed, and following the direct injection of antigenic substance, structural changes were noted in the crypt epithelium. A cold light laryngoscope tube was used to inject ovalbumin or bovine serum albumin, plus Freund's complete adjuvant, into the subepithelial lymphatic tissue. Five weeks later subcutaneous challenge injections of the same protein produced increased numbers and proportions of infiltrated small lymphocytes and medium-sized lymphocytes containing a highly organized granular endoplasmic reticulum. These cells occupied wide intercellular passageways. Epithelial plasma membranes that faced these passageways remained smooth, but other surfaces of the same epithelial cells acquired vastly increased numbers of microvilli. The surfaces of other epithelial cells that faced each other also showed microvilli. These microvilli faced expanded interfacial canals. Increased numbers of small lymphocytes were observed emigrating through postcapillary venules immediately beneath the epithelium. The microvilli and other fine structures of tonsillar crypt epithelial cells are compared with similar structures of epithelial cells of the thymus. The experimentally induced increase in microvilli suggests the possibility that tonsillar crypt epithelial cells make a secretory contribution to local immune reactions.

scholarly journals Adenoviral Gizzard Erosion in Commercial Broiler Chickens

2003 ◽  
Vol 40 (3) ◽  
pp. 294-303 ◽  
Author(s):  
M. Ono ◽  
Y. Okuda ◽  
S. Yazawa ◽  
Y. Imai ◽  
I. Shibata ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Inclusion Bodies ◽  
Broiler Chickens ◽  
Plasma Cells ◽  
Muscle Layer ◽  
Intranuclear Inclusion ◽  
Group I ◽  
Serotype 1 ◽  
Commercial Broiler ◽  
Antibody Levels

Pathologic and immunohistochemical changes caused by group I of the fowl adenovirus (FAV) serotype-1 99ZH strain, isolated from broiler chickens exhibiting gizzard erosion, were investigated in commercial broiler chickens. One hundred twenty-two chickens were inoculated with the strain by both oral and ocular routes at 1, 3, or 5 weeks of age and euthanatized for necropsy within 4–18 days of inoculation. Focal gizzard erosions were observed in the inoculated chickens of each age group. A histologically degenerative koilin layer, necrotic mucosa, intranuclear inclusion bodies in the glandular epithelial cells, inflammatory cell infiltrations in the lamina propria, submucosa, and a muscle layer were seen in the gizzards. Immunohistochemical staining showed evidence of FAV antigens in the intranuclear inclusion bodies. These findings were recognized regardless of their maternal antibody levels for FAV serotype-1. Gizzard lesions appeared later in the lower-dose-inoculated chickens than in the higher-dose–inoculated chickens. Numerous CD3-positive cells and IgY-positive plasma cells were seen in the gizzard lesions. In 5-week-old chickens the heterophil infiltrations in the lesions were milder than in younger chickens. Intranuclear inclusion bodies also were observed in the epithelial cells of the ileum or cecal tonsils of some chickens. Thus, this study shows that FAV-99ZH causes adenoviral gizzard erosion in broiler chickens without hepatic or pancreatic lesions and that cell infiltration is more severe than in dietary gizzard erosions.

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