scholarly journals Some Analytical Aspects of the Immunochemical Determination of the Selectivity of Proteinuria

Author(s):  
R. Beetham ◽  
R. J. Mills ◽  
D. N. Raine ◽  
R. H. R. White

The selectivity of proteinuria has been determined from the relative clearances of α1-acid glycoprotein, albumin, transferrin and IgG in 190 children and adolescents. The precision of the determination of selectivity (C.V. = 3%) is greater than that of the individual protein clearances determined by a double immunodiffusion method (C.V. = 13–22%). Neither sample storage for a limited period nor concentrating the urine affects the selectivity value significantly; serial determinations in one patient for 24 h showed no diurnal variation. The effectiveness of selectivity in predicting the steroid response in the nephrotic syndrome was found to be slightly superior when determined from all four proteins than from transferrin and IgG alone. Inclusion of α2-macroglobulin relative clearance in the selectivity determination often significantly alters the value determined from the other four proteins alone. The reasons for this are discussed. Demonstration of α2M in urine which has not been concentrated, strongly suggests the presence of a structural glomerular lesion.

1973 ◽  
Vol 56 (6) ◽  
pp. 1475-1479 ◽  
Author(s):  
Ugo R Cieri

Abstract Sulfaquinoxaline is determined by its UV absorbance at about 358 nm, where the other 3 sulfonamides do not absorb. Sulfathiazole, sulfamerazine, and sulfamethazine are determined by a quantitative TLC procedure, based on the separation of the compounds on silica gel plates; the spots are extracted and the centrifuged extracts are analyzed spectro-photometrically. A method of calculating the total sulfonamide content, independent of the individual components, is also introduced.


2008 ◽  
Vol 53 (No. 9) ◽  
pp. 421-431 ◽  
Author(s):  
K. Ryglová

This paper deals with problems of rural tourism development. The works is focused on researching the situation in the area of rural tourism in the Czech Republic, on understanding the attitudes of entrepreneurial sphere and rural population to this entrepreneurship and mainly on the determination of limiting factors that prevent this form of tourism from a more distinctive development. Rural tourism in the Czech Republic is still in the initiating stage of its development and it is not as developed as in some countries in West Europe. This goal has been reached with the help of primary questionnaire inquiry among business and agricultural subjects in rural areas in the individual regions and the detail results of this questionnaire inquiry are enclosed to the following paper. These identified factors were mainly insufficient financial means and at the same time insufficient state assistance connected with this. Insufficient awareness of this support as well as its inaccessibility for small business appears to be a difficulty, too. From the other obstacles, we can mention the current legislation system, bureaucracy, the state of communication and tourist infrastructure, fears of neighbours’ intolerance and losing privacy and also unfamiliarity and little experience.


1945 ◽  
Vol 23a (4) ◽  
pp. 57-72 ◽  
Author(s):  
R. Ruedy

When the soil is assumed to consist of two layers—the upper of resistivity ρ1 and the lower of resistivity ρ2—and cumulative resistances are calculated by adding or integrating the earth-resistivity functions for intervals that are a fraction of the thickness of the upper layer, a practically linear relation is obtained between the cumulative resistance and the electrode spacing until the distance between the electrodes is equal to the thickness of the upper material. Should one of the materials be at least twice as conducting as the other, the extent of the deviation from the linear law enables the determination of the depth of the upper stratum and of the ratio between the resistivities of the two layers. When three layers are present and the middle layer is at least twice as thick as the top stratum, the thicknesses may be deduced from the two departures of the cumulative resistances from the linear law. Since these conclusions are based on the theory of the individual apparent resistivity of stratified ground at various electrode spacings, they have the same range of application as the earth-resistivity curves, but the occurrence of straight line graphs facilitates the plotting and the interpretation of results based on a necessarily limited number of measurements.


1984 ◽  
Vol 67 (6) ◽  
pp. 1044-1052 ◽  
Author(s):  
Leon Prosky ◽  
Nils-Georg Asp ◽  
Ivan Furda ◽  
Jonathan W Devries ◽  
Thomas F Schweizer ◽  
...  

Abstract An interlaboratory study was conducted to determine the total dietary fiber (TDF) content of food, food products, and total diets, using a combination of enzymatic and gravimetric procedures. Thirteen unknown products including 2 mixed diets (one lacto-ovo vegetarian and the other nonvegetarian) were analyzed by 32 analysts. Duplicate samples of dried foods were gelatinized with Termamyl, a heat-stable alphaam) lase, and then enzymatically digested with protease and amyloglucosidase to remove the protein and starch present in the sample. Four volumes of 95% ethanol were added to precipitate the soluble dietary fiber. The total residue was filtered, and then washed with 74% ethanol, 95% ethanol, and acetone. After drying, the residue was weighed. One of the duplicates was analyzed for protein, and the other was ashed at 525°C and the ash was measured. TDF was calculated as the weight of the residue less the weight of protein and ash. Coefficients of variation for 10 of the samples ranged from 2.95 to 26.39%. For 3 of the samples high coefficients of variation were obtained. The results compared satisfactorily with those obtained previously by the best method available for the individual foods studied.


2020 ◽  
Vol 11 (4) ◽  
pp. 20-40
Author(s):  
Dominika Doktorová ◽  
◽  
Nikola Piteková ◽  

The main goal of this work is to compare the personality characteristics in individual types of perfectionism. In order to determine the perfectionism, we used Frost’s Multidimensional Perfectionism Scale (F-MPS) and NEO five-factor personal inventory (NEO-FFI) for personal characteristics. There questionnaires were administered to humanistic science students (N=344) in the age span of 19 to 26. Through the non-hierarchical aggregate analyse we identified three types of perfectionists in the sample: functional, dysfunctional perfectionists and non-perfectionists. The comparison of the individual typed of perfectionism with personality characteristics showed statistically significant differences in the following way: compared to the other two type the dysfunctional perfectionists achieved the highest score on the scale of neuroticism. Other differences between the perfectionist types did not show any statistical importance.


1917 ◽  
Vol s2-62 (248) ◽  
pp. 555-611
Author(s):  
J. BRONTÉ GATENBY

(1) The ovotestis of Helix aspersa is formed of fingerlike diverticula. The latter are hollow at their lower ends, which connect to the hermaphrodite duct, while the upper ends contain more yolk, and are filled completely with metamorphosing male cells. (2) According to the manner of derivation--that is, the nutrimental conditions of the locality from which new cells arise, and the number (if any) of times which these new cells divide--there are quite wide differences in the individual generations derived from and under such varying conditions. (3) These differences are found in nucleus, mitochondria, Nebenkern, and general cell volume. (4) The mitochondria vary in size and number, and such variation seems to be caused by the varying number of spermatogonial divisions in different regions of the ovotestis. (5) In the early spermatid smaller mitochondria, the micromitochondria appear in an unknown way near the region from which the axial filament takes its origin from the centro some applied to the nucleus. These micromitochondria are about one-fourth the size of the other, or macromitochondria. No perceptible variation in size of the micromitochondria of various generations has been found. (6) The micromitochondria form the front sheath of the sperm; the hind region of the micromitochondrial sheath intercommunicates with the macromitochondrial sheath, which follows behind. (7) The Nebenkern does not apparently become absorbed into the substance of the mitochondrial sheath. A sloughing off appears to take place. (8) The minute cytology of the derivation of the sperms, eggs, and nurse-cells is described. (9) The determination of the sex of the indifferent cell seems to be brought about by a variety of causes. The explanation of femaleness by presence of yolk cells is held to be inadequate, for male progerminative cells also appear in regions choked with yolk. (10) The probable function of the Nebenkern is discussed.


1945 ◽  
Vol 39 (2) ◽  
pp. 350-355 ◽  
Author(s):  
Margaret Spahr

Does the concept of sovereignty under law necessarily involve a self-contradiction? That it does, has admittedly been held by the great majority of careful thinkers from the time of Hobbes to the present day. Nor has this been inconsequential. The belief that submission to an enforceable law would be a surrender of sovereignty has been a most potent obstacle to the substitution of the law court for the battlefield in the determination of international disputes. On the other hand, it is generally conceded that for the individual the only liberty worth seeking is liberty under law. It is the thesis of this article that sovereignty under law for the state is no more absurd than liberty under law for the individual.The term “sovereignty” has been variously and elaborately defined, but for present purposes its essential elements may be listed as authority, equality, and liberty. The first-named attribute—authority—has its great importance in the field of constitutional law, which postulates that in every state there is some agency or combination of agencies possessed of the authority to control everything within the state. However, it is well known that the rise and spread of constitutionalism and federalism have rendered the concept of sovereign authority increasingly mystical. Even in Great Britain, the old simplicity of the sovereignty of “King in Parliament” has been complicated by the Parliament Act of 1911, and especially by the Statute of Westminster of 1931. To-be sure, it is easy enough to visualize sovereign authority in a dictatorial régime, but this arouses no envy on the part of those who enjoy other forms of government.


1965 ◽  
Vol 64 (3) ◽  
pp. 373-378 ◽  
Author(s):  
H. S. Bayley ◽  
D. Lewis

The apparent and corrected digestibilities of beef tallow, H.E.F. and soyabean oil have been determined in the newly weaned pig. Determinations of the individual fatty acids in the food and faecal lipids allowed the digestibility of each acid to be calculated separately. The purest available forms of oleic, stearic and palmitic acids and their corresponding triglycerides were also used in the determination of digestibility. Although the digestibility of each fatty acid is to some extent a characteristic of the acid, the extent of absorption can also be modified by the other acids in the diet. The results.of these experiments give no clear indication of a difference between feeding fatty acids in the free form or as the triglycerides. The digestible energy values of the fats have been calculated as the product of gross energy value and corrected digestibility.


IG ◽  
2021 ◽  
Vol 44 (4) ◽  
pp. 251-265
Author(s):  
Manuel Müller

Purpose narratives play an important role in the legitimization of the European Union (EU). Three goals attributed to the EU have been especially prominent: inner peace, prosperity and self-assertion on the world stage. However, all three can only inadequately justify the supranational character of European integration. A stronger justification is offered by the cosmopolitan-democratic narrative, according to which the purpose of the EU is the individual and collective self-determination of citizens beyond national borders. The cosmopolitan-democratic narrative is historically more recent and has mostly been less salient in the public debate than the other three, but nevertheless has had an important political impact on the development of the EU. Like the other narratives, however, it is not undisputed and has been the focus of various controversial debates since the 1990s.


Author(s):  
D.R. Rasmussen ◽  
N.-H. Cho ◽  
C.B. Carter

Domains in GaAs can exist which are related to one another by the inversion symmetry, i.e., the sites of gallium and arsenic in one domain are interchanged in the other domain. The boundary between these two different domains is known as an antiphase boundary [1], In the terminology used to describe grain boundaries, the grains on either side of this boundary can be regarded as being Σ=1-related. For the {110} interface plane, in particular, there are equal numbers of GaGa and As-As anti-site bonds across the interface. The equilibrium distance between two atoms of the same kind crossing the boundary is expected to be different from the length of normal GaAs bonds in the bulk. Therefore, the relative position of each grain on either side of an APB may be translated such that the boundary can have a lower energy situation. This translation does not affect the perfect Σ=1 coincidence site relationship. Such a lattice translation is expected for all high-angle grain boundaries as a way of relaxation of the boundary structure.


Sign in / Sign up

Export Citation Format

Share Document