Prevention of Alveolar Bone Loss in an Osteoporotic Animal Model via Interference of Semaphorin 4d

Semaphorin 4d (Sema4d) has been proposed as a novel target gene for the treatment of osteoporosis. Recently, we fabricated a site-specific bone-targeting system from polymeric nanoparticles that demonstrates an ability to prevent bone loss in an osteoporotic model by interfering with Sema4d gene expression using small interference RNA (siRNA) molecules. The aim of the present investigation was to determine the effects of this targeting system on the periodontium, an area of high bone turnover. We demonstrated, by single photon emission computed tomography, that intravenous injection of this molecule in ovariectomized Balb/C mice is able to target alveolar bone peaking 4 hr post-injection. We then compared, by histological analysis, the bone volume/total volume (BV/TV), alveolar bone height loss, immunohistochemical expression of Sema4d, and total number of osteoclasts in mandibular alveolar bone. Four treatment modalities were compared as follows: (1) sham-operated, (2) OVX-operated, (3) OVX+estrogen replacement therapy, and (4) OVX+siRNA-Sema4d animals. The results from the present study demonstrate that an osteoporotic condition significantly increases alveolar bone height loss, and that the therapeutic effects via bone-targeting systems featuring interference of Sema4d are able to partly counteract alveolar bone loss caused by osteoporosis. While the future therapeutic demand for the large number of patients suffering from osteoporosis faces many challenges, we demonstrate within the present study an effective drug-delivery moiety with anabolic effects on the bone remodeling cycle able to locate and target alveolar bone regeneration.

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Continuous Periosteal Strapping Sutures for Stabilization of Osseous Grafts With Resorbable Membranes for Buccal Ridge Augmentation: A Technique Report

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-17-00060 ◽

2017 ◽

Vol 43 (4) ◽

pp. 283-290 ◽

Cited By ~ 3

Author(s):

Tamir H Shalev ◽

Gregori M Kurtzman ◽

Alon H Shalev ◽

Deborah K Johnson ◽

Mark Elias M Kersten

Keyword(s):

Bone Loss ◽

Tooth Loss ◽

Alveolar Bone ◽

Effective Means ◽

Alveolar Bone Loss ◽

Socket Preservation ◽

Ridge Structure ◽

Ridge Augmentation ◽

Novel Technique ◽

Bone Height

Alveolar bone loss occurs after extraction with loss of a premolar or anterior tooth; the residual supporting alveolar bone loss averages 1.53 mm of crestal bone height and 3.87 mm of buccolingual width, with most of the bone loss occurring at the facial plate. Socket preservation does not completely preserve the original ridge contours but can be an effective means of reducing bone loss following extraction. Attempts to rebuild the alveolar ridge structure after tooth loss often employ the concept of guided bone regeneration, a technique-sensitive procedure that routinely involves placement of particulate bone with or without fixation screws and either a resorbable or a nonresorbable membrane. We present a novel technique for stabilizing a resorbable membrane and underlying particulate graft allowing for predictable bone grafting across multiple edentulous sites.

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Therapeutic effects of caffeic acid phenethyl ester on alveolar bone loss in rats with endotoxin-induced periodontitis

Journal of Dental Sciences ◽

10.1016/j.jds.2019.03.011 ◽

2019 ◽

Vol 14 (4) ◽

pp. 339-345

Author(s):

Alper Kızıldağ ◽

Taner Arabacı ◽

Mevlüt Albayrak ◽

Ufuk Taşdemir ◽

Erman Şenel ◽

...

Keyword(s):

Bone Loss ◽

Caffeic Acid ◽

Alveolar Bone ◽

Caffeic Acid Phenethyl Ester ◽

Alveolar Bone Loss ◽

Therapeutic Effects

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Inhibition of RANKL and Sema4d Improves Residual Ridge Resorption in Mice.

10.21203/rs.3.rs-1209410/v1 ◽

2022 ◽

Author(s):

Meri Hisamoto ◽

Shunsuke Kimura ◽

Kai Iwata ◽

Toshihiko Iwanaga ◽

Atsuro Yokoyama

Keyword(s):

Gene Expression ◽

Bone Loss ◽

Molecular Mechanisms ◽

Alveolar Bone ◽

Alveolar Bone Loss ◽

Period Gene ◽

Semaphorin 4D ◽

Long Time ◽

Extraction Model

Abstract Residual ridge resorption (RRR) is a chronic and progressive bone resorption following tooth loss. It causes deterioration of the oral environments and leads to the pathogenesis of various systemic diseases. However, the molecular mechanisms and risk factors for RRR progression are still unclear and controversial. In this study, we developed a tooth extraction model using mice for analyzing long-term morphological and gene expression changes in the alveolar bone. We further applied ovariectomy to this model to elucidate the effects of osteoporosis on RRR progression. As a result, the alveolar bone loss was biphasic and consisted of rapid loss in the early stages and subsequently slow and sustained bone loss over a long period. Gene expression analysis indicated that ovariectomy increased the expression of pro-inflammatory cytokines in the alveolar bone and prolonged the activation of osteoclasts same as histological analysis. Furthermore, the expressions of Tnfsf11 and Sema4d kept increasing for a long time in OVX mice. Administration of neutralization antibodies for receptor activator of NF-κB ligand (RANKL) effectively suppressed RRR. Similarly, inhibition of Semaphorin 4d (Sema4d) also improved alveolar bone loss. This study demonstrated that osteoporosis is a risk factor for RRR and that RANKL and Sema4d suppression are potential treatments.

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Tulang alveolar pasca perawatan jembatan ditinjau dari radiografi periapikalAlveolar bone after bridge treatment in terms of periapical radiography

Padjadjaran Journal of Dental Researchers and Students ◽

10.24198/pjdrs.v4i1.24043 ◽

2020 ◽

Vol 4 (1) ◽

pp. 9

Author(s):

Celine Hestiana ◽

Ria Noerianingsih Firman ◽

Deddy Firman

Keyword(s):

Bone Loss ◽

Alveolar Bone ◽

Alveolar Bone Loss ◽

Gingival Inflammation ◽

Probing Depth ◽

Before And After ◽

Dental Hospital ◽

Bone Height ◽

Periapical Radiography ◽

Denture Treatment

Pendahuluan: Radiografi periapikal dapat digunakan untuk mendeteksi tulang alveolar pada perawatan gigi tiruan jembatan. Pembuatan gigi tiruan jembatan harus memenuhi syarat-syarat biologis. Apabila adaptasi marginal buruk, penempatan margin intracrevicular terlalu dalam, permukaan restorasi yang kasar dan restorasi yang overkontur dapat menyebabkan peradangan lokal seperti inflamasi gingiva, peningkatan kedalaman probing dan kehilangan tulang alveolar. Tujuan penelitian ini untuk mengetahui bagaimana deskripsi tulang alveolar pasca perawatan jembatan di RSGM UNPAD ditinjau dari radiografi periapikal. Metode: Jenis penelitian deskriptif dengan populasi adalah seluruh hasil radiograf periapikal pada pasien sebelum dan sesudah perawatan jembatan posterior yang datang ke RSGM Unpad dan sampel adalah radiograf sebelum dan sesudah perawatan jembatan posterior sebanyak 17 pasien setelah insersi 1 minggu dan 2 pasien setelah insersi 4 minggu. Pengukuran ketinggian tulang alveolar menggunakan Metode Proksimal RABL (Radiographic Alveolar Bone Loss) yang dihitung memakai jangka sorong. Hasil : Kehilangan tulang alveolar setelah insersi 1 minggu pada mesial dan distal yaitu 0,003 mm, setelah insersi 2 minggu 0,007 mm pada mesial dan 0,025 pada distal,setelah insersi 3 minggu 0,025 mm pada mesial dan 0,019 mm pada distal, setelah insersi 4 minggu 0,075 mm mesial dan 0,063 mm distal. Simpulan: Pasca perawatan jembatan, ditinjau dari radiografi periapikal, terdapat kehilangan tulang alveolar ringan berdasarkan metode proksimal RABL.Kata kunci : Gigi tiruan jembatan, radiograf periapikal, tulang alveolar. ABSTRACTIntroduction: Periapical radiography can be used to detect alveolar bone in bridge denture treatment. The making of bridge denture must fulfil biological requirements. Poor marginal adaptation will lead to deeper intracrevicular margin placement, rough surfaces, over contour restoration can cause local inflammation such as gingival inflammation, increased probing depth and alveolar bone loss. The purpose of this research was to know the description of alveolar bone after bridge denture treatment reviewed through periapical radiography. Methods: This research was descriptive with the population of all radiograph periapical treatment of posterior bridge in Universitas Padjadjaran Dental Hospital and the sample was radiograph periapical before and after bridge treatment of as much as 17 patients after 1-week insertion and 2 patients after 4-weeks insertion. Alveolar bone height measurements was carried out using the RABL (Radiographic Alveolar Bone Loss) proximal method calculated using the Vernier callipers. Results: The results indicated that alveolar bone loss occurred after 1-week insertion on the mesial and distal was 0.003 mm; after 2-weeks insertion was 0.007 mm on the mesial and 0.025 mm on the distal; after 3-weeks insertion was 0.025 mm on the mesial and 0.019 mm on the distal; and after 4-weeks insertion was 0.075 mm on the mesial and 0.063 mm on the distal. Conclusion: Post bridge treatment, in terms of periapical radiography, found a mild alveolar bone loss based on the proximal RABL method. Keywords: Bridge denture, periapical radiograph, alveolar bone.

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Probiotics Ameliorate Alveolar Bone Loss by Regulating Gut Microbiota

10.21203/rs.3.rs-97356/v1 ◽

2020 ◽

Author(s):

Leming Jia ◽

Ye Tu ◽

Xiaoyue Jia ◽

Qian Du ◽

Xin Zheng ◽

...

Keyword(s):

Bone Loss ◽

Gut Microbiota ◽

Alveolar Bone ◽

Critical Role ◽

Alveolar Bone Loss ◽

Estrogen Deficiency ◽

Long Bone ◽

Therapeutic Effects ◽

Gut Permeability ◽

Ovx Rats

Abstract BackgroundEstrogen deficiency is an etiological factor of postmenopausal osteoporosis (PMO), which not only decreases bone density in vertebrae and long bone, but also aggravates inflammatory bone loss in alveolar bone. Recent evidence has suggested the critical role of gut microbiota in osteoimmunology, and modulation of gut microbiota may have positive influence on bone metabolisms. The present study aimed to evaluate the therapeutic effects of probiotics on alveolar bone loss under estrogen-deficient condition. Inflammatory alveolar bone loss induced by either chronic periodontitis or apical periodontitis was established in ovariectomized (OVX) rats, which were gavage-fed with probiotics daily until sacrifice. Gut microbiota and gut permeability, as well as alveolar bone loss and the related osteoimmune were evaluated to investigate the effects and underlying mechanisms by which probiotics counter the alveolar bone loss under estrogen-deficiency. ResultsWe found that administration of probiotics significantly prevented periodontal and apical bone resorption in OVX rats. Administration of probiotics significantly enriched butyrate-producing genera and enhanced gut butyrate production, resulting in improved intestinal barrier and decreased gut permeability in the OVX rats. Furthermore, the estrogen deprivation-induced inflammatory responses were suppressed in probiotics-treated OVX rats, as reflected by reduced serum levels of inflammatory cytokines and a balanced distribution of CD4+IL-17A+Th17 cells and CD4+CD25+Foxp3+Treg cells in the bone marrow. ConclusionOur data demonstrate that probiotics can effectively attenuate alveolar bone loss by modulating gut microbiota and further regulating osteoimmune, and thus represent a promising adjuvant in the treatment of alveolar bone loss under estrogen-deficiency.

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EFFECTS OF ALENDRONATE THERAPY ON CYCLOSPORINE INDUCED ALVEOLAR BONE LOSS : A MORPHO-METRIC AND BIO-CHEMICAL STUDY IN RATS

International Journal of Advanced Research ◽

10.21474/ijar01/193 ◽

2016 ◽

Vol 4 (4) ◽

pp. 947-955

Author(s):

Sneha R Bhat ◽

◽

Aravind R Kudva ◽

Dhoom S Mehta ◽

◽

...

Keyword(s):

Bone Loss ◽

Alveolar Bone ◽

Chemical Study ◽

Alveolar Bone Loss

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3,4,5-Trihydroxybenzoic acid attenuates ligature-induced periodontal disease in Wistar rats.

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry ◽

10.2174/1871523019666200206094335 ◽

2020 ◽

Vol 19 ◽

Author(s):

Ozkan Karatas ◽

Fikret Gevrek

Keyword(s):

Bone Loss ◽

Gallic Acid ◽

Wistar Rats ◽

Alveolar Bone ◽

Alveolar Bone Loss ◽

Collagenase Activity ◽

Osteoblastic Activity ◽

Cell Counts ◽

Experimental Periodontitis ◽

Anti Inflammatory

Background: 3,4,5-Trihydroxybenzoic acid, which is also known as gallic acid, is an anti-inflammatory agent who could provide beneficial effects in preventing periodontal inflammation. The present study aimed to evaluate the anti-inflammatory effects of gallic acid on experimental periodontitis in Wistar rats. Alveolar bone loss, osteoclastic activity, osteoblastic activity, and collagenase activity were also determined. Methods: 32 Wistar rats were used in the present study. Study groups were created as following: Healthy control (C,n=8) group; periodontitis (P,n=8) group; periodontitis and 30 mg/kg gallic acid administered group (G30,n=8); periodontitis and 60 mg/kg gallic acid administered group (G60,n=8). Experimental periodontitis was created by placing 4-0 silk sutures around the mandibular right first molar tooth. Morphological changes in alveolar bone were determined by stereomicroscopic evaluation. Mandibles were undergone histological evaluation. Matrix metalloproteinase (MMP)-8, tissue inhibitor of MMPs (TIMP)-1, bone morphogenetic protein (BMP)-2 expressions, tartrate-resistant acid phosphatase (TRAP) positive osteoclast cells, osteoblast, and inflammatory cell counts were determined. Results: Highest alveolar bone loss was observed in the periodontitis group. Both doses of gallic acid decreased alveolar bone loss compared to the P group. TRAP-positive osteoclast cell counts were higher in the P group, and gallic acid successfully lowered these counts. Osteoblast cells also increased in gallic acid administered groups. Inflammation in the P group was also higher than those of C, G30, and G60 groups supporting the role of gallic acid in preventing inflammation. 30 and 60 mg/kg doses of gallic acid decreased MMP-8 levels and increased TIMP-1 levels. BMP levels increased in gallic acid administered groups, similar to several osteoblasts. Conclusion: Present results revealed an anti-inflammatory effect of gallic acid, which was indicated by decreased alveolar bone loss and collagenase activity and increased osteoblastic activity.

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Gram-positive bacteria cell wall-derived lipoteichoic acid induces inflammatory alveolar bone loss through prostaglandin E production in osteoblasts

Scientific Reports ◽

10.1038/s41598-021-92744-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Tsukasa Tominari ◽

Ayumi Sanada ◽

Ryota Ichimaru ◽

Chiho Matsumoto ◽

Michiko Hirata ◽

...

Keyword(s):

Cell Wall ◽

Bone Loss ◽

Alveolar Bone ◽

Osteoclast Differentiation ◽

Lipoteichoic Acid ◽

Alveolar Bone Loss ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria

AbstractPeriodontitis is an inflammatory disease associated with severe alveolar bone loss and is dominantly induced by lipopolysaccharide from Gram-negative bacteria; however, the role of Gram-positive bacteria in periodontal bone resorption remains unclear. In this study, we examined the effects of lipoteichoic acid (LTA), a major cell-wall factor of Gram-positive bacteria, on the progression of inflammatory alveolar bone loss in a model of periodontitis. In coculture of mouse primary osteoblasts and bone marrow cells, LTA induced osteoclast differentiation in a dose-dependent manner. LTA enhanced the production of PGE2 accompanying the upregulation of the mRNA expression of mPGES-1, COX-2 and RANKL in osteoblasts. The addition of indomethacin effectively blocked the LTA-induced osteoclast differentiation by suppressing the production of PGE2. Using ex vivo organ cultures of mouse alveolar bone, we found that LTA induced alveolar bone resorption and that this was suppressed by indomethacin. In an experimental model of periodontitis, LTA was locally injected into the mouse lower gingiva, and we clearly detected alveolar bone destruction using 3D-μCT. We herein demonstrate a new concept indicating that Gram-positive bacteria in addition to Gram-negative bacteria are associated with the progression of periodontal bone loss.

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