Mandibular Condylectomy in the Guinea Pig: Quantitative Triple Fluorochrome Study

1976 ◽  
Vol 55 (5) ◽  
pp. 848-853 ◽  
Author(s):  
Narendar N. Soni ◽  
Randolph B. Malloy
Keyword(s):  
Bone Formation ◽  
Guinea Pig ◽  
Surface Area ◽  
Guinea Pigs ◽  
Total Surface Area ◽  
And Control

Different-colored fluorochromes were administered sequentially to guinea pigs and the rate of bone formation measured in their condylectomized and control contralateral hemimandibles. The differences in values of condylectomized and control contralateral sides for demethylchlortetracycline- and total surface area-labeled bone were statistically signifrcant but were not significant for 2,5-bis [N-N1 -di-(Carbomethyl) aminomethyl]- and hematoporphyrin-labeled bone.

scholarly journals EFEKTIVITAS PEMBERIAN GEL BINAHONG (ANREDERA CORDIFOLIA) 5% TERHADAP JUMLAH SEL FIBROBLAST PADA SOKET PASCA PENCABUTAN GIGI MARMUT (CAVIA COBAYA)

2015 ◽  
Vol 2 (1) ◽  
pp. 64 ◽  
Author(s):  
Tifani Ardiana ◽  
Andina Rizkia Putri Kusuma ◽  
Muhammad Dian Firdausy
Keyword(s):  
Treatment Group ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Tooth Extraction ◽  
Control Group ◽  
Fibroblast Cells ◽  
Not Given ◽  
Significant Difference ◽  
And Control ◽  
Group 1

Background: Binahong Leaf (Anredera Cordifolia) is known as a medicinal plant. Binahong gel contains an assortment of phytochemicals. Purpose: This study was conducted to determine the effectiveness of the Binahong gel (Anredera cordifolia) 5% application into the socket after tooth extraction of guinea pig (Cavia cobaya), and then its fibroblast cells histologically observed at days 4 and 7. Method: This research method was a quasi-experimental. The sample used were 16 guinea pigs (Cavia cobaya) males, aged 3-5 months and divided into 4 groups. Group 1 consisted of four guinea pigs were given a gel Binahong until day 4, 1 control group consisted of four guinea pigs were not given gel Binahong until day 4, 2  treatment groups were given gel Binahong until day 7 and 2 control groups were not given gel Binahong until day 7, then the guenia pigs decapitated to be made histological preparats, the aim to see and count the number of fibroblast cells. Kemudian dimasukan dalam data lalu dianalisis. The data analyzed by Kruskal Wallis test with probability p <0.05, and Mann Whitney to determine differences in effectiveness between variables. Result: Based on the Kruskal-Wallis test, there is a significant difference between the number of fibroblasts which had giving Binahong gel after tooth extraction of guinea pigs (p <0.05). From the Mann Whitney test concluded the treatment group 1 and control group 1 showed not significant (p> 0.05). In the second treatment group and control group 2 can be concluded there is a significant difference (p <0.05). Conclusion: From the results of this study application gel Binahong 5% for 4 days after tooth extraction does not effectively influence the number of fibroblasts, for 7 days proved effective influence fibroblast cell count after tooth extraction of guinea pig (Cavia cobaya).

ANTI-BRUCELLA SERUM: GUINEA PIG PROTECTION EXPERIMENTS

1938 ◽  
Vol 16d (12) ◽  
pp. 353-360 ◽  
Author(s):  
Ronald Gwatkin
Keyword(s):  
Guinea Pig ◽  
Brucella Abortus ◽  
Guinea Pigs ◽  
Rabbit Serum ◽  
Natural Manner ◽  
The Third ◽  
And Control

Guinea pigs were protected against infection with Brucella abortus by intraperitoneal injections of fresh, unpreserved, anti-abortus rabbit serum. In the first two experiments the serum-treated and control animals were exposed to infection by contact with infected guinea pigs, while in the third experiment they were exposed by instillation of the infecting organism into the eye. In the first experiment 60% of the controls and none of the treated animals were infected. In the second, 80% of the controls and 20% of the treated animals were infected. In the third, all of the treated animals were protected, while all of the controls became infected. The former method, although less certain than the latter, is worthy of consideration in experiments of this type, as it permits infection to occur in a natural manner.

scholarly journals 29 COMPARISON OF TERM PLACENTAS IN CLONED AND CONTROL PREGNANCIES IN CATTLE

2005 ◽  
Vol 17 (2) ◽  
pp. 164 ◽  
Author(s):  
C.A. Batchelder ◽  
M. Bertolini ◽  
K.A. Hoffert ◽  
J.B. Mason ◽  
A.L. Moyer ◽  
...  
Keyword(s):  
Surface Area ◽  
Fetal Loss ◽  
Fold Increase ◽  
Total Surface Area ◽  
Placental Development ◽  
Ovarian Cell Lines ◽  
Term Delivery ◽  
Type C ◽  
And Control

Somatic cell nuclear transfer is associated with high incidence of fetal loss, late-term pregnancy complications, perinatal mortality, and abnormal placental development. Several groups have described abnormalities of early and mid-gestation cloned placentas (Hill et al. 2000 Biol. Reprod. 63, 1787–1794; Lee et al. 2004 Biol. Reprod. 70, 1–11). The objective of our study was to characterize differences in the placentas of clones and control calves at term delivery. Clones were produced from ovarian cell lines from two donors (Holstein, n = 5; Hereford, n = 2). Breed-matched controls included AI (Holstein, n = 3) and embryo transfer (Holstein, n = 3; Hereford n = 3) calves. All calves were delivered alive with no visible birth defects between Days 273 and 280 of gestation, and placentas were recovered for measurement and morphological analysis. When possible, pregnancies were delivered via caesarian section, and the entire uterus was recovered for classification of anatomical shape of placentomes. Each placentome was measured, weighed, and classified by type as (A) engulfing mushroom-like; (B) sub-engulfing mushroom-like; (C) flattened, non-engulfing; and (D) convex (adapted from Penninga and Longo 1998 Placenta 19, 187–193, for sheep). Mean number of placentomes per placenta was significantly greater in controls than clones, while total mass of placentomes in the pregnant horn was significantly greater in clones than in controls (Table 1). Total surface area of placentomes in the pregnant horn tended to be larger and more variable in clones (range: 2710–7450 cm2) than in controls (range: 3120–5030 cm2; P < 0.10). A two-fold increase was observed in cloned placentas, as compared with control placentas, in mean surface area per placentome and mass per placentome. Anatomically, cloned placentas differed from controls in the percentage of placentomes classified Type A (controls > clones) and Type C (clones > controls). Other abnormalities noted in cloned placentas included moderate to severe edema, teratomas, enlarged vessels, and large areas devoid of placentation. All clones and 2/9 controls displayed enlarged umbilical vessels. Significant placental abnormalities were observed in all cloned pregnancies. Table 1. Placental characteristics of term cloned and control pregnancies

Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

1983 ◽  
Vol 41 ◽  
pp. 726-727
Author(s):  
Corazon D. Bucana
Keyword(s):  
Bone Marrow ◽  
Guinea Pig ◽  
Electron Density ◽  
Peritoneal Cavity ◽  
Ultrastructural Study ◽  
Guinea Pigs ◽  
Random Distribution ◽  
Glycogen Content ◽  
Polymorphonuclear Neutrophils ◽  
Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

Inhibition of Human and Animal Platelet Adhesiveness to Glass Bead Columns by Adenosine, Dipyridamole, Chlorpromazine and Acetylsalicylic Acid

1976 ◽  
Vol 36 (02) ◽  
pp. 401-410 ◽  
Author(s):  
Buichi Fujttani ◽  
Toshimichi Tsuboi ◽  
Kazuko Takeno ◽  
Kouichi Yoshida ◽  
Masanao Shimizu
Keyword(s):  
Guinea Pig ◽  
Acetylsalicylic Acid ◽  
Guinea Pigs ◽  
Glass Bead ◽  
Animal Species ◽  
Inhibitory Effect ◽  
Rabbit Platelet ◽  
Platelet Adhesiveness

SummaryThe differences among human, rabbit and guinea-pig platelet adhesiveness as for inhibitions by adenosine, dipyridamole, chlorpromazine and acetylsalicylic acid are described, and the influence of measurement conditions on platelet adhesiveness is also reported. Platelet adhesiveness of human and animal species decreased with an increase of heparin concentrations and an increase of flow rate of blood passing through a glass bead column. Human and rabbit platelet adhesiveness was inhibited in vitro by adenosine, dipyridamole and chlorpromazine, but not by acetylsalicylic acid. On the other hand, guinea-pig platelet adhesiveness was inhibited by the four drugs including acetylsalicylic acid. In in vivo study, adenosine, dipyridamole and chlorpromazine inhibited platelet adhesiveness in rabbits and guinea-pigs. Acetylsalicylic acid showed the inhibitory effect in guinea-pigs, but not in rabbits.

INCORPORATION OF IODIDE INTO ORGANIC COMPOUNDS IN THE GUINEA PIG THYROID

1963 ◽  
Vol 43 (1) ◽  
pp. 110-118 ◽  
Author(s):  
R. Ekholm ◽  
T. Zelander ◽  
P.-S. Agrell
Keyword(s):  
Guinea Pig ◽  
Protein Binding ◽  
Organic Compounds ◽  
Guinea Pigs ◽  
Microsomal Fraction ◽  
Thyroid Tissue ◽  
Particulate Fraction ◽  
Supernatant Fraction ◽  
Hydrolysis Of

ABSTRACT Guinea pigs, kept on a iodine-sufficient diet, were injected with Na131I and the thyroids excised from 45 seconds to 5 days later. The thyroid tissue was homogenized and separated into a combined nuclear-mitochondrial-microsomal fraction and a supernatant fraction by centrifugation at 140 000 g for one hour. Protein bound 131iodine (PB131I) and free 131iodide were determined in the fractions and the PB131I was analysed for monoiodotyrosine (MIT), diiodotyrosine (DIT) and thyroxine after hydrolysis of PB131I. As early as only 20 minutes after the Na131I-injection almost 100% of the particulate fraction 131I was protein bound. In the supernatant fraction the protein binding was somewhat less rapid and PB131I values above 90% of total supernatant 131I were not found until 3 hours after the injection. In all experiments the total amount of PB131I was higher in the supernatant than in the corresponding particulate fraction. The ratio between supernatant PB131I and pellet PB131I was lower in experiments up to 3 minutes and from 2 to 5 days than in experiments of 6 minutes to 20 hours. Hydrolysis of PB131I yielded, even in the shortest experiments, both MIT and DIT. The DIT/MIT ratio was lower in the experiments up to 2 hours than in those of 3 hours and over.

Method for recording ECG's in unanesthetized guinea pigs

1965 ◽  
Vol 20 (5) ◽  
pp. 1091-1093 ◽  
Author(s):  
Alfred Richtarik ◽  
Thomas A. Woolsey ◽  
Enrique Valdivia
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Standing Posture ◽  
Factors Affecting ◽  
Rapid Succession ◽  
Unanesthetized Animals ◽  
Animal Size ◽  
Four Electrodes

A device for use in recording ECG's from guinea pigs is described. It is constructed of Plexiglas and consists of a base with four electrodes (separated by plastic ridges) on which the animal stands. The animal's activity is restricted by a removable box, the ends and top of which are adjustable to compensate for variations in animal size. The device permits recording of ECG's in rapid succession from quiet, unanesthetized animals in normal standing posture. Results obtained with the method are reported. apparatus for guinea pig ECG; time relations guinea pig ECG; normal ECG, guinea pig; factors affecting quality of ECG recordings from guinea pigs Submitted on October 21, 1964

scholarly journals Intravitreal brimonidine inhibits form-deprivation myopia in guinea pigs

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Yifang Yang ◽  
Junshu Wu ◽  
Defu Wu ◽  
Qi Wei ◽  
Tan Zhong ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Intravitreal Injection ◽  
Guinea Pigs ◽  
Intravitreal Injections ◽  
Eye Drops ◽  
Rna Seq ◽  
Myopia Progression ◽  
Expression Levels ◽  
Guinea Pig Model ◽  
Administration Methods

Abstract Background The use of ocular hypotensive drugs has been reported to attenuate myopia progression. This study explores whether brimonidine can slow myopia progression in the guinea pig form-deprivation (FD) model. Methods Three-week-old pigmented male guinea pigs (Cavia porcellus) underwent monocular FD and were treated with 3 different methods of brimonidine administration (eye drops, subconjunctival or intravitreal injections). Four different concentrations of brimonidine were tested for intravitreal injection (2 μg/μL, 4 μg/μL, 20 μg/μL, 40 μg/μL). All treatments continued for a period of 21 days. Tonometry, retinoscopy, and A-scan ultrasonography were used to monitor intraocular pressure (IOP), refractive error and axial length (AL), respectively. On day 21, guinea pigs were sacrificed for RNA sequencing (RNA-seq) to screen for associated transcriptomic changes. Results The myopia model was successfully established in FD animals (control eye vs. FD eye, respectively: refraction at day 20, 0.97 ± 0.18 D vs. − 0.13 ± 0.38 D, F = 6.921, P = 0.02; AL difference between day 0 and day 21, 0.29 ± 0.04 mm vs. 0.45 ± 0.03 mm, F = 11.655, P = 0.004). Among the 3 different brimonidine administration methods, intravitreal injection was the most effective in slowing myopia progression, and 4 μg/μL was the most effective among the four different concentrations of brimonidine intravitreal injection tested. The AL and the refraction of the brimonidine intravitreal injection group was significantly shorter or more hyperopic than those of other 2 groups. Four μg/μL produced the smallest difference in AL and spherical equivalent difference values. FD treatment significantly increased the IOP. IOP was significantly lower at 1 day after intravitreal injections which was the lowest in FD eye of intravitreal injection of brimonidine. At day 21, gene expression analyses using RNA-seq showed upregulation of Col1a1 and Mmp2 expression levels by intravitreal brimonidine. Conclusions Among the 3 different administration methods, intravitreal injection of brimonidine was the most effective in slowing myopia progression in the FD guinea pig model. Intravitreal brimonidine at 4 μg/μL significantly reduced the development of FD myopia in guinea pigs. Expression levels of the Col1a1 and Mmp2 genes were significantly increased in the retinal tissues of the FD-Inj-Br group.

scholarly journals A SOLUBLE ANTIGEN OF LYMPHOCYTIC CHORIOMENINGITIS

1940 ◽  
Vol 72 (4) ◽  
pp. 389-405 ◽  
Author(s):  
J. E. Smadel ◽  
M. J. Wall
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Lymphocytic Choriomeningitis ◽  
The Other ◽  
Soluble Antigen ◽  
Soluble Substance ◽  
Other Hand ◽  
The Times

Anti-soluble substance antibodies and neutralizing substances, which develop following infection with the virus of lymphocytic choriomeningitis, appear to be separate entities. The times of appearance and regression of the two antibodies are different in both man and the guinea pig; the antisoluble substance antibodies appear earlier and remain a shorter time. Moreover, mice develop them but no demonstrable neutralizing substances. Injection of formalin-treated, virus-free extracts containing considerable amounts of soluble antigen fails to elicit anti-soluble substance antibodies and to induce immunity in normal guinea pigs; administration of such preparations to immune pigs, however, is followed by a marked increase in the titer of anti-soluble substance antibodies in their serum. On the other hand, suspensions of formolized washed virus are effective in normal guinea pigs in stimulating both anti-soluble substance antibodies and protective substances, and in inducing immunity to infection.

scholarly journals Trichuris spp. in Animals, with Specific Reference to Neo-Tropical Rodents

2021 ◽  
Vol 8 (2) ◽  
pp. 15
Author(s):  
Kegan Romelle Jones
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Clinical Signs ◽  
Molecular Techniques ◽  
Meat Production ◽  
Specific Reference ◽  
Hydrochoerus Hydrochaeris ◽  
Present Information ◽  
Low Prevalence ◽  
A New Species

Trichuriasis is the clinical disease of animals infected with the parasite of the genus Trichuris. This review attempts to present information on Trichuris spp. infestation in neo-tropical rodents that are utilized for meat consumption by humans. Neo-tropical rodents utilized for meat production can be divided into two categories: those that have been domesticated, which include the guinea pig (Cavia porcellus), and those that are on the verge of domestication, such as the capybara (Hydrochoerus hydrochaeris), lappe (Cuniculus paca/Agouti paca), and agouti (Dasyprocta leporina). This document reviews the literature on the species of Trichuris that affects the rodents mentioned above, as well as the clinical signs observed. The literature obtained spans over sixty years, from 1951 to 2020. Trichuris spp. was found in these neo-tropical rodents mentioned. However, there is a dearth of information on the species of Trichuris that parasitize these animals. The capybara was the only rodent where some molecular techniques were used to identify a new species named T. cutillasae. In most cases, Trichuris spp. was found in combination with other endoparasites, and was found at a low prevalence in the lappe and guinea pig. The presence of Trichuris spp. ranged from 4.62–53.85% in the agouti, 4.21–10.00% in the lappe, 50% in the capybaras, and 1–31% in guinea pigs. Further work must be done towards molecular identification of various Trichuris spp. present in these rodents, as well as the clinical effect of infection on the performance of agouti, lappe, capybara, and guinea pigs.

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