Pulmonary Endothelial Cell Modifications after Storage in Solid-Organ Preservation Solutions

1995 ◽  
Vol 23 (3) ◽  
pp. 200-206 ◽  
Author(s):  
P Carbognani ◽  
L Spaggiari ◽  
M Rusca ◽  
L Cattelani ◽  
P Solli ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Organ Preservation ◽  
Ultrastructural Changes ◽  
Osmic Acid ◽  
Solid Organ ◽  
Ringer Lactate ◽  
University Of Wisconsin ◽  
Transmission Electron ◽  
Grading Scale

During lung preservation, the vascular endothelium is probably the first site of damage and these lesions are considered the main limiting factor in solid-organ preservation. In the present study, the ultrastructural changes in the endothelial cells of human pulmonary artery hypothermically stored (at 4 °C) for 6 and 12 h in Euro-Collins, University of Wisconsin and Ringer-lactate solutions were compared. The arteries obtained from three patients who underwent pneumonectomy were divided into 20 segments and preserved in the three solutions mentioned. The specimens, which were fixed in osmic acid, were examined using transmission electron microscopy. Transmission electron microscopy indicated that the cells stored in the University of Wisconsin solution either for 6 or 12 h were the best preserved, while the most severely damaged cells were those stored in Euro-Collins solution, even after just 6 h. The cells stored in Ringer-lactate showed an intermediate level of damage. The data from an ultrastructural grading scale, which quantified the damage to the cytoplasm, mitochondria and nucleus, were in broad agreement with the general transmission electron microscopy observations. Analysis of variance of the grading scale data showed that there were statistically significant differences between the groups after both 6 and 12 h storage ( P < 0.05).

Comparative Study of the Fine Morphology of Sensory Receptors in Aspidogaster conchicola and Cotylaspis insignis

1972 ◽  
Vol 30 ◽  
pp. 150-151
Author(s):  
Venita F. Allison ◽  
J. E. Ubelaker ◽  
J. H. Martin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Nervous System ◽  
Osmic Acid ◽  
Sensory Receptors ◽  
Transmission Electron ◽  
Sensory Structures ◽  
Fine Morphology ◽  
Scanning Electron

It has been suggested that parasitism results in a reduction of sensory structures which concomitantly reflects a reduction in the complexity of the nervous system. The present study tests this hypothesis by examining the fine morphology and the distribution of sensory receptors for two species of aspidogastrid trematodes by transmission and scanning electron microscopy. The species chosen are an ectoparasite, Cotylaspis insignis and an endoparasite, Aspidogaster conchicola.Aspidogaster conchicola and Cotylaspis insignis were obtained from natural infections of clams, Anodonta corpulenta and Proptera purpurata. The specimens were fixed for transmission electron microscopy in phosphate buffered paraformaldehyde followed by osmic acid in the same buffer, dehydrated in an ascending series of ethanol solutions and embedded in Epon 812.

scholarly journals Pollen, Tapetum, and Orbicule Development inColletia paradoxaandDiscaria americana(Rhamnaceae)

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
M. Gotelli ◽  
B. Galati ◽  
D. Medan
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Ultrastructural Changes ◽  
Transmission Electron ◽  
The Family ◽  
Tapetal Cells ◽  
Grain Formation

Tapetum, orbicule, and pollen grain ontogeny inColletia paradoxaandDiscaria americanawere studied with transmission electron microscopy (TEM). The ultrastructural changes observed during the different stages of development in the tapetal cells and related to orbicule and pollen grain formation are described. The proorbicules have the appearance of lipid globule, and their formation is related to the endoplasmic reticulum of rough type (ERr). This is the first report on the presence of orbicules in the family Rhamnaceae. Pollen grains are shed at the bicellular stage.

Ultrastructural Changes in the Compound Middle Lamella of Pinus thunbergii During Lignification and Lignin Removal

Holzforschung ◽  
2000 ◽  
Vol 54 (3) ◽  
pp. 234-240 ◽  
Author(s):  
Jonas Hafrén ◽  
Takeshi Fujino ◽  
Takao Itoh ◽  
Ulla Westermark ◽  
Noritsugu Terashima
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Middle Lamella ◽  
Pinus Thunbergii ◽  
Ultrastructural Changes ◽  
Woody Tissue ◽  
Compound Middle Lamella ◽  
Lignin Removal ◽  
Transmission Electron ◽  
Early Phases

SummaryThe structure of the middle lamella inPinus thunbergiihas been studied by the rapid-freeze deep-etching (RFDE) technique in combination with transmission electron microscopy (TEM). The ultrastructure of the compound middle lamella was studied in the early phases of the development of woody tissue in the cambial and differentiating xylem, before the heavy incrustation with lignin had occurred. Lignified middle lamella in the xylem was studied both directly and after delignification. It was found that the structure of the unlignified middle lamella in the cambium/developing xylem consists of a fine irregular network probably containing pectin and hemicellulose. As a result of lignin incrustation, the middle lamella becomes increasingly dense and the surface structure of the fully lignified middle lamella appeared to be compact and partly covered with globular structures. After delignification of the lignified middle lamella a thin network with a different structure was revealed. This network probably mainly consists of hemicellulose. No microfibrils of the type that occurs in the primary and secondary walls were found in the middle lamella.

The Technology in Sample Preparation for Transmission Electron Microscopy and Ultrastructure Observation of Lung Tissue in Rats with Experimental Silicosis

2014 ◽  
Vol 875-877 ◽  
pp. 695-698
Author(s):  
Qian Li ◽  
Li Juan Zhang ◽  
Fang Yang ◽  
Wen Li Zhang ◽  
Hong Xu
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Sample Preparation ◽  
Success Rate ◽  
Cutting Speed ◽  
Ultrastructural Changes ◽  
Histological Changes ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Perfusion Fixation

ts hard to get ideal ultrathin sections because of the adamant SiO2 dust in silicosis, after perfusion fixation methods and strict control of the cutting speed, improving the success rate of the Silicosis tissue TEM sample preparation of ultrathin sections,so we can more clearly and accurately observed ultrastructural changes of silicosis,and it also can offer morphological basis for research the silicosis organizations function histological changes.

Effects Of Varying Salinity On Leaf Ultrastructure Of Potamogeton Pectinatus L

1999 ◽  
Vol 5 (S2) ◽  
pp. 1256-1257
Author(s):  
A.D. Barnabas ◽  
P. Bunsi ◽  
Y. Naidoo ◽  
W.J. Przybylowicz ◽  
J. Mesjasz-Przybylowicz
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
High Salinity ◽  
Ultrastructural Changes ◽  
Potamogeton Pectinatus ◽  
Leaf Epidermis ◽  
Leaf Ultrastructure ◽  
Low Salinity ◽  
Transmission Electron ◽  
Standard Procedures

Potamogeton pectinatus is a submerged halophyte which occurs in waters of low salinity (5% to 10%). Its upper salinity tolerance has been reported to be 19%. Reasons why P.pectinatus is unable to tolerate salinities in excess of 19%is important to our understanding of its biology. In the present study, leaf ultrastructure of plants growing at low salinity was compared with plants growing at high salinity in order to assess the effects of different salinities on the ultrastructure. Attention was focussed on ultrastructural changes occurring in the leaf epidermis, the main photosynthetic tissue.Plants were grown in seawater at two salinities : 5%(low salinity) and 20% (high salinity). Pieces of mature leaf blades from both treatments were harvested and prepared for Transmission Electron Microscopy (TEM) following standard procedures. The overall distribution and concentration of chlorine (CI) in the leaves was ascertained since this element is the most abundant anion in seawater and is important in considerations of salt tolerance in submerged halophytes.

scholarly journals Changes in Ultrastructure and Fourier Transform Infrared Spectrum of Salmonella enterica Serovar Typhimurium Cells after Exposure to Stress Conditions

2010 ◽  
Vol 76 (22) ◽  
pp. 7598-7607 ◽  
Author(s):  
A. Alvarez-Ordóñez ◽  
M. Prieto
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fourier Transform ◽  
Stress Response ◽  
Salmonella Enterica ◽  
Fourier Transform Infrared ◽  
Ultrastructural Changes ◽  
Transmission Electron ◽  
Serovar Typhimurium ◽  
Ft Ir

ABSTRACT The effect of exposure to acid (pH 2.5), alkaline (pH 11.0), heat (55°C), and oxidative (40 mM H2O2) lethal conditions on the ultrastructure and global chemical composition of Salmonella enterica serovar Typhimurium CECT 443 cells was studied using transmission electron microscopy and Fourier transform infrared spectroscopy (FT-IR) combined with multivariate statistical methods (hierarchical cluster analysis and factor analysis). Infrared spectra exhibited marked differences in the five spectral regions for all conditions tested compared to those of nontreated control cells, which suggests the existence of a complex bacterial stress response in which modifications in a wide variety of cellular compounds are involved. The visible spectral changes observed in all of the spectral regions, together with ultrastructural changes observed by transmission electron microscopy and data obtained from membrane integrity tests, indicate the existence of membrane damage or alterations in membrane composition after heat, acid, alkaline, and oxidative treatments. Results obtained in this study indicate the potential of FT-IR spectroscopy to discriminate between intact and injured bacterial cells and between treatment technologies, and they show the adequacy of this technique to study the molecular aspects of bacterial stress response.

Changes in the plastid ultrastructure during greening in cultured soybean cells

1989 ◽  
Vol 47 ◽  
pp. 1010-1011
Author(s):  
M.A. Gillott ◽  
G. Erdös ◽  
D. E. Buetow
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Gene Regulation ◽  
Uranyl Acetate ◽  
Total Darkness ◽  
Ultrastructural Changes ◽  
Ethanol Dehydration ◽  
Mesophyll Cells ◽  
Plastid Ultrastructure ◽  
Transmission Electron

Mesophyll cells isolated from soybeans (Glycine max, L. Merr. var. Corsoy) can be grown photoautotrophically in suspension culture. The SB-P cell line can be bleached by maintaining them in total darkness in sucrose supplemented media for several weeks, and will regain photosynthetic competency when returned to the light. This system is ideally suited for the study of gene regulation and the biochemical and ultrastructural changes which occur during the greening process.Cells were fixed for electron microscopy after 8 weeks of growth in total darkness and at intervals of 1 h to 12 d after transfer to the light. Chlorophyll measurements were determined for each sample. For transmission electron microscopy, the cells were fixed for 1-2 h in 4% glutaraldehyde in 0.1M Pipes buffer, pH 7.4, washed in the same buffer, then postfixed for 1 h in 1% OsO4 in Pipes, pH 6.8. Following a graded ethanol dehydration series the cells were transferred into propylene oxide and embedded in Epon. Sections were stained with uranyl acetate and observed on a JEOL 100C TEM operated at 80 kV.

Ultrastructural changes in Staphylococcus aureus treated with pulsed electric fields / Cambios ultraestructurales en Staphylococcus aureus sometida a campos eléctricos pulsantes

1997 ◽  
Vol 3 (2) ◽  
pp. 113-121 ◽  
Author(s):  
U.R. Pothakamury ◽  
G.V. Barbosa-Cánovas ◽  
B.G. Swanson ◽  
K.D. Spence
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Electric Field ◽  
Electric Fields ◽  
Untreated Control ◽  
Ultrastructural Changes ◽  
Transmission Electron ◽  
Treat Ment

Early stationary phase cells of Staphylococcus aureus were inoculated into a model food, simulated milk ultrafiltrate (SMUF) and subjected to 16, 32, and 64 pulses at electric field intensities of 20, 40 and 60 kV/cm at 13 °C. In addition temperatures of 20, 25 and 30 °C were also tested with 32 pulses and an electric field of 60 kV/cm. The temperature of the SMUF increased by 1-2 ° C at the end of the 64 pulses. Cells subjected to 64 pulses at 20, 40 and 60 kV/cm were observed for ultrastructural changes using scanning and transmission electron microscopy techniques. The cell surface was rough after treatment with electric field when observed by scanning electron microscopy (SEM). The cell wall was broken, and the cytoplasmic contents were leaking out of the cell after exposure to 64 pulses at 60 kV/cm when observed by transmission electron microscopy (TEM). The breaking of the cell wall is an indication of electro-mechanical breakdown of the cell. The increase in inactivation with an increase in the electric field strength can be related to the increase in the damage to the cells. Cells subjected to 32 pulses at 60 kV/cm and 13, 20 or 25 °C were compared microscopically with the untreated control cells. Cells subjected to heat treat ment (10 min, at 66 °C) were compared with electric field-treated and untreated control cells. Although important changes were observed in the protoplast, no cell wall breakdown was observed in heat-treated cells when compared to the electric field-treated cells. This result indi cates a different mechanism of inactivation of cells with heat treatment.

Cell wall differentiation during early somatic embryogenesis in plants. II. Ultrastructural study and pectin immunolocalization on chicory embryos

2000 ◽  
Vol 78 (6) ◽  
pp. 824-831 ◽  
Author(s):  
Audrey Chapman ◽  
Anne-Sophie Blervacq ◽  
Théo Hendriks ◽  
Christian Slomianny ◽  
Jacques Vasseur ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Cell Wall ◽  
Somatic Embryogenesis ◽  
Cell Walls ◽  
Ultrastructural Changes ◽  
Outer Cell ◽  
Embryogenic Cell ◽  
Transmission Electron ◽  
Outer Cell Wall

In Cichorium hybrid clone 474 (C. intybus L. var. sativum × C. endivia L. var. latifolia), direct somatic embryogenesis was induced from roots. Using transmission electron microscopy, we followed the ultrastructural changes of the outer cell wall in relation to embryo developmental stage. During the transition from an embryogenic cell to a somatic embryo, the differentiation of the outer cell wall involved both deposition and rearrangement processes. During the first divisions, the cell wall of few-celled embryos still enclosed in the root tissue appeared as a large amorphous layer of cellulose, thicker than the cell walls of the root cortex cells. When the proembryo emerged from the root cells, the outer wall surface exhibited a fibrillar material designated as the supraembryonic network. As this network disappeared, the outer cell wall changed organization, and two domains were distinguished. At the torpedo stage, the outer cell wall was more compact without any gaps and the protoderm was differentiated. Immunolocalization of an epitope recognised by JIM5 antibody revealed the unesterified nature of the supraembryonic network. Such pectins were also located at the outer third of the outer cell wall of protodermal cells as well as in the intercellular spaces. Highly methylesterified pectins recognized by JIM7 antibodies were slightly present in the cell walls during the embryogenesis process. The different stages of the outer cell wall differentiation as well as the development of the transient supraembryonic network are described, and its possible roles in somatic embryogenesis are proposed.Key words: cell differentiation, cell wall, Cichorium (chicory), pectin, somatic embryogenesis, transmission electron microscopy.

Sign in / Sign up

Export Citation Format

Share Document