scholarly journals Intravenous Chlormethiazole — Haemolysis with Concentrated Solutions

1981 ◽  
Vol 9 (1) ◽  
pp. 34-39 ◽  
Author(s):  
W. B. Runciman ◽  
A. H. Ilsley ◽  
R. G. Ryall ◽  
K. S. Parkin ◽  
R. Heinrich
Keyword(s):  
Inferior Vena ◽  
The Other ◽  
Blood Levels ◽  
Central Vein ◽  
Concentrated Solutions ◽  
Plasma Haemoglobin ◽  
Free Plasma Haemoglobin ◽  
Free Plasma ◽  
Infusion Tubing

Since the current clinical concentration of chlormethiazole solutions (0.8%) may require the infusion of large volumes of fluid, it was decided to examine the effects on haemolysis of infusing higher concentrations of chlormethiazole into a central vein. Approximately one gram of chlormethiazole was infused into the inferior vena cavae of six anaesthetised greyhounds over each half hour using, successively, 0.8%, 1.2%, 2%, 5%, 10%, and 20% solutions of chlormethiazole. Free plasma haemoglobin levels were measured at five minute intervals, and blood chlormethiazole levels at 15 minute intervals. A rapidly progressive haemolysis occurred when the 5 or 10% solutions were infused. In a further four greyhounds, one gram of chlormethiazole was infused over each half hour using a 0.8% solution, whilst progressively hyperosmolar dextrose solutions were infused at the same rates in succeeding half hours as the concentrated chlormethiazole solutions had been infused in the first six dogs. No haemolysis occurred in these control animals. Chlormethiazole blood levels were similar in each group. Loss of chlormethiazole into the infusion tubing was examined and found to be 20% for the 0.8% solution, and 10% for the 1.2% solution, but was insignificant with the other subsequently infused concentrations of chlormethiazole. It is concluded that rapid progressive haemolysis occurs in association with the infusion of chlormethiazole solutions when concentrations of greater than 5 or 10% are infused into the inferior vena cavae of anaesthetised greyhounds.

scholarly journals Lung Function Abnormalities in Sickle Cell Anaemia

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Yvonne A. Dei-Adomakoh ◽  
Jane S. Afriyie-Mensah ◽  
Audrey Forson ◽  
Martin Adadey ◽  
Thomas A. Ndanu ◽  
...  
Keyword(s):  
Steady State ◽  
Lung Function ◽  
Sickle Cell ◽  
Clinical History ◽  
Cross Sectional Study ◽  
Cell Disease ◽  
Cross Sectional ◽  
Plasma Haemoglobin ◽  
Free Plasma Haemoglobin ◽  
Free Plasma

Background. Abnormalities in lung function tests have been shown to commonly occur in a majority of patients with sickle cell disease (SCD) even at steady state. The prevalence and pattern of these lung function abnormalities have been described in other populations but this is unknown among our sickle cell cohort. There is generally little information available on risk factors associated with the lung function abnormalities and its relevance in patient care. Method. This was an analytical cross-sectional study involving 76 clinically stable, hydroxyurea-naive adult Hb-SS participants and 76 nonsickle cell disease (non-SCD) controls. A structured questionnaire was used to obtain sociodemographic data and clinical history of the participants. Investigations performed included spirometry, pulse oximetry, tricuspid regurgitant jet velocity (TRV) measurements via echocardiogram, complete blood counts, free plasma haemoglobin, serum urea, and creatinine. Results. Weight, BMI, mean FVC, and FEV1% predicted values were comparatively lower among the Hb-SS patients (p < 0.001). Abnormal spirometry outcome occurred in 70.4% of Hb-SS patients, predominantly restrictive defects (p < 0.001), and showed no significant association with steady-state Hb, WBC count, free plasma haemoglobin, frequency of sickling crisis, chronic leg ulcers, and TRV measurements (p > 0.05). The mean oxygen saturation was comparatively lower among Hb-SS patients (p < 0.001). Conclusion. Measured lung volumes were significantly lower in Hb-SS patients when compared to non-SCD controls and this difference was not influenced by anthropometric variance. Lung function abnormalities, particularly restrictive defects, are prevalent in Hb-SS patients but showed no significant association with recognized markers of disease severity.

Extracellular vesicles in the circulation: are erythrocyte microvesicles a confounder in the plasma haemoglobin assay?

2013 ◽  
Vol 41 (1) ◽  
pp. 288-292 ◽  
Author(s):  
Karen M.K. de Vooght ◽  
Cedric Lau ◽  
Pim P.M. de Laat ◽  
Richard van Wijk ◽  
Wouter W. van Solinge ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Formation Rate ◽  
Haemoglobin Concentration ◽  
Cell Types ◽  
Total Cell ◽  
Plasma Haemoglobin ◽  
Free Plasma Haemoglobin ◽  
Free Haemoglobin ◽  
Different Cell Types ◽  
Free Plasma

Blood contains a mixture of extracellular vesicles from different cell types, primarily platelets, endothelial cells, leucocytes and erythrocytes. Erythrocytes are the most abundant cell type in blood and could, especially in certain pathologies, represent an important source of vesicles. Since erythrocytes contain the haemoglobin components iron and haem, which are potentially toxic, it is important to investigate the contribution of vesicle-associated haemoglobin to total cell-free haemoglobin levels. To our knowledge, this is the first time that cell-free plasma haemoglobin has been differentiated into vesicle-associated and molecular species. We investigated the contribution of vesicle-associated haemoglobin in residual patient material that was routinely analysed for total cell-free plasma haemoglobin. All patient samples included in the study were haemolytic with total cell-free haemoglobin concentration ranging from 80 to 2500 mg/l. In the majority of the samples, total cell-free haemoglobin concentration was between 100 and 200 mg/l. No haemoglobin could be detected in the vesicle fraction, indicating that the contribution of vesicle-associated haemoglobin to total cell free-haemoglobin levels in plasma is negligible. It is important to investigate whether erythrocyte vesicles are not formed in blood or that their production is not increased during pathologies associated with haemolysis or that the clearance rate of the vesicles surpasses the formation rate.

Haemolysis during cardiopulmonary bypass: how to reduce the free haemoglobin by managing the suctioned blood separately

Perfusion ◽  
2001 ◽  
Vol 16 (6) ◽  
pp. 519-524 ◽  
Author(s):  
A Pierangeli ◽  
V Masieri ◽  
F Bruzzi ◽  
E De Toni ◽  
G Grillone ◽  
...  
Keyword(s):  
Cardiopulmonary Bypass ◽  
Artery Bypass ◽  
Bypass Graft ◽  
Control Group ◽  
Study Group ◽  
Artery Bypass Graft ◽  
Operative Field ◽  
Free Plasma Haemoglobin ◽  
Free Plasma

During cardiopulmonary bypass (CPB) the collection of the patient’s blood from the operating area is of fundamental importance. This blood is collected in the cardiotomy reservoir using field suckers and can be managed in different ways. It can be filtered in the cardiotomy reservoir and redirected to the venous reservoir, then oxygenated and returned to the patient, or it can be managed separately: collected in the cardiotomy reservoir, treated at the end of the operation and only after this, returned to the patient. The aim of this study is to determine in vivo the effect of a separate management of the suction blood from the operative field, using the Avant D903 oxygenator (Dideco, Mirandola, Italy). Twenty-one patients undergoing coronary artery bypass graft surgery with CPB were selected and put into two groups at random. In the control group ( n 10) the suction blood in the cardiotomy reservoir was filtered and immediately redirected into the venous reservoir, oxygenated and returned to the patient. In the study group ( n 11) the suctioned blood was collected in the D903 Avant’s (Dideco) cardiotomy reservoir and returned to the patient only after having been washed at the end of the operation, using a Compact Advanced (Dideco), as required. Clinical data demonstrated that while in the study group it was possible to keep the free plasma haemoglobin (FPH) concentrations the same as at the beginning, in the control group there was a significant increase in FPH from 5.0 3.5 mg/dl (baseline) to 37 16.7 mg/dl (120 min after CPB).

scholarly journals Association of Peripheral Blood Levels of Cytokines With Autism Spectrum Disorder: A Meta-Analysis

2021 ◽  
Vol 12 ◽  
Author(s):  
Huaying Zhao ◽  
Hongqi Zhang ◽  
Shijie Liu ◽  
Wulin Luo ◽  
Yongfeng Jiang ◽  
...  
Keyword(s):  
Autism Spectrum Disorder ◽  
Peripheral Blood ◽  
Meta Analysis ◽  
Autism Spectrum ◽  
Spectrum Disorder ◽  
The Other ◽  
Sensitivity Analyses ◽  
Systemic Review ◽  
Blood Levels ◽  
Random Effects Models

Background: Although increasing evidence suggests an association between alterations in peripheral cytokines and autism spectrum disorder (ASD), a consensus is lacking. To determine whether abnormal cytokine profiles in peripheral blood were associated with ASD, we performed this systemic review and meta-analysis.Methods: A systematic literature search was conducted through the Embase, PubMed, Web of Knowledge, PsycINFO, and Cochrane databases up to 4 June 2020. Clinical studies exploring the aberration of peripheral cytokines of autistic patients and controls were included in our meta-analysis. We pooled extracted data using fixed- or random-effects models based on heterogeneity tests with Comprehensive Meta-analysis software. We converted standardized mean differences to Hedges' g statistic to obtain the effect sizes adjusted for sample size. Subgroup analyses, sensitivity analyses, meta-regression, and publication bias tests were also carried out.Results: Sixty-one articles (326 studies) were included to assess the association between 76 cytokines and ASD. We conducted our meta-analysis based on 37 cytokines with 289 studies. Since there were fewer than three studies on any of the other 39 cytokines, we only provided basic information for them. The levels of peripheral IL-6, IL-1β, IL-12p70, macrophage migration inhibitory factor (MIF), eotaxin-1, monocyte chemotactic protein-1 (MCP-1), IL-8, IL-7, IL-2, IL-12, tumor necrosis factor-α (TNF-α), IL-17, and IL-4 were defined as abnormal cytokines in the peripheral blood of ASD patients compared with controls. The other 24 cytokines did not obviously change in ASD patients compared with the controls.Conclusions: The findings of our meta-analysis strengthen the evidence for an abnormal cytokine profile in ASD. These abnormal cytokines may be potential biomarkers for the diagnosis and treatment of ASD in the future.

scholarly journals Influence of Glutamine and Glutamate Supplementation in the Blood Levels of Horses

2019 ◽  
Vol 47 (1) ◽  
Author(s):  
Helena Emília Cavalcanti da Costa Cordeiro Manso ◽  
Deyvson Ferreira De Oliveira ◽  
Monica Miranda Hunka ◽  
Hélio Cordeiro Manso Filho
Keyword(s):  
Amino Acid ◽  
Latin Square ◽  
Dietary Supplementation ◽  
The Body ◽  
The Other ◽  
Glutamine Metabolism ◽  
Free Form ◽  
Control Group ◽  
Blood Levels ◽  
Total Plasma Protein

Background: The most abundant free amino acid in mammals is glutamine (GLN). Little research has focused on GLN supplementation for horses, but GLN levels in this species are known to decline after exercise and during lactation. Under physiological conditions, the body produces Gln in sufficient quantities for general metabolism, and a small part of this amino acid comes from dietary protein. Little research has so far focused on equine dietary supplementation with free glutamine or combined with other amino acids during catabolic states or in highly stressful situations. This research was conducted to evaluate the effects of equine dietary supplementation using a combination of glutamine and glutamate.  Materials, Methods & Results: The study involved four Arabian mares, not in training (~380 kg; ~12 years old) and four treatments (control, and inclusions of 1, 2 and 4% of GLN+GLU) in a Latin square model. A 7-day washout period was established between each phase. Fifty percent of the mares’ maintenance energy requirements came from concentrate and 50% from hay and grazing. The other 50% came from Tifton hay (Cynodon dactylon), which was supplied ad libitum. After 7 weeks of nutritional supplementation (once a day, in the morning). In the experimental model, the mares were distributed in a Latin square design comprised of four treatments: control (without inclusion) and inclusions of 1%, 2% and 4% of supplement (AminoGut®, Ajinomoto do Brazil), and four animals. Blood was collected in five stages (fasting, and 60, 120, 240 and 360 min after feeding) in each treatment. The blood samples were analyzed to determine GLN, GLU, urea, creatinine, uric acid, total plasma protein, hematocrit and glucose levels. Glutamine and Glutamate concentrations were analyzed using the enzymatic spectrophotometric method. The results were analyzed statistically using one- and two-way ANOVA and Tukey’s test with P set at 5%. The results indicated that GLN differed in both the group (P < 0.001) and between the phases of supplementation (P < 0.001), but no interaction occurred between them (P > 0.05). Significant changes in GLN levels were also observed in the 4% inclusion treatment compared to all the treatments in the fasting phase and in the + 60 min and +240 min phases of the control group (P < 0.05). All the other biomarkers analyzed here were unchanged (Glutamate, Urea, Creatinine, Urea, TPP, Glucose and Hematocrit) (P > 0.05) during the period under analysis, and remained within the normal range for the species in their current stabling conditions. The mares presented no clinical problems nor did they change their feeding behavior during the supplementation period or on the days blood was collected.Discussion: Glutamine metabolism in horses has yet to be extensively studied.  However, it has been shown that, when supplied to horses in its free form, this amino acid causes Gln levels to rise rapidly within the first 90 min of the postprandial period.  This indicates that an extra amount of this amino acid may increase Gln blood levels despite intense degradation of enterocytes. This study found that supplementation with a combination of Gln+Glu can increase blood Gln levels after 6 h in the treatment involving 4% Gln+Glu included in the concentrate (P < 0.05) In conclusion, supplementation with GLN+GLU raised the mares’ GLN levels after 360 min when 4% of GLN+GLU was included in their diet. These results may be used to establish GLN supplementation models for horses. 

Increase in intake with sham feeding experience is concentration dependent

1999 ◽  
Vol 277 (2) ◽  
pp. R565-R571 ◽  
Author(s):  
John D. Davis ◽  
Gerard P. Smith ◽  
Bramdeo Singh ◽  
Daniel P. McCann
Keyword(s):  
Gastrointestinal Tract ◽  
Negative Feedback ◽  
The Other ◽  
Feedback Signal ◽  
Dilute Solution ◽  
Concentrated Solutions ◽  
Sweetened Condensed Milk ◽  
Sham Feeding ◽  
Feeding Experience ◽  
Condensed Milk

Most sham feeding studies show that about three sham feeding tests are required for intake to reach maximum. One study, however, using a dilute solution, reported maximum sham intake in the first sham feeding test, suggesting that the progressive rise in sham intake may be concentration dependent. We tested this hypothesis with six groups of rats given five sham feeding tests each with one of six concentrations of sweetened condensed milk (0.5:1, 1:1, 2:1, 4:1, 8:1, 16:1, water-to-milk dilutions). It took three sham tests for intake to reach maximum with the three most concentrated solutions, but only one with the three weakest. Thus the intake of concentrated solutions of milk is limited by two negative feedback signals, one derived from the accumulation of fluid in the gastrointestinal tract, the other from a labile signal that loses its effectiveness with sham feeding experience. In contrast, the intake of weak concentrations is limited only by the nonlabile negative feedback signal because the labile signal is missing.

Suprathermal plasma distribution functions with relativistic cut-offs

2019 ◽  
Vol 491 (3) ◽  
pp. 3967-3973
Author(s):  
H-J Fahr ◽  
M Heyl
Keyword(s):  
Distribution Function ◽  
Heat Transport ◽  
Distribution Functions ◽  
The Other ◽  
Pressure Reduction ◽  
Plasma Distribution ◽  
Order Of Magnitude ◽  
Low Mass ◽  
Velocity Moments ◽  
Free Plasma

ABSTRACT In typical plasma physics scenarios, when treated on kinetic levels, distribution functions with suprathermal wings are obtained. This raises the question of how the associated typical velocity moments, which are needed to arrive at magnetohydrodynamic plasma descriptions, may appear. It has become evident that the higher velocity moments in particular, for example the pressure or heat transport, which are constructed as integrations of the distribution function, contain unphysical contributions from particles with velocities greater than the velocity of light. In what follows, we discuss two possibilities to overcome this problem. One is to calculate a maximal, physically permitted, upper velocity, which can be realized in view of the underlying energization processes, and to stop the integration there. The other is to modify the distribution function relativistically so that no particles with superluminal (v ≥ c) velocities appear. On the basis of a typical collision-free plasma scenario, like the plasma in the heliosheath, we obtain the corresponding expressions for electron and proton pressures and can show that in both cases the pressures are reduced compared with their classical values; however, electrons experience a stronger reduction than protons. When calculating pressure ratios, it turns out that these are of the same order of magnitude regardless of which of the two methods is used. The electron, as the low-mass particle, undergoes the more pronounced pressure reduction. It may turn out that electrons and protons constitute about equal pressures in the heliosheath, implying that no pressure deficit need be claimed here.

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