Cytotoxicity of long-term denture base materials

2018 ◽  
Vol 41 (10) ◽  
pp. 677-683 ◽  
Author(s):  
Merve Çakırbay Tanış ◽  
Canan Akay ◽  
Handan Sevim
Keyword(s):  
Cell Viability ◽  
Cytotoxic Effect ◽  
Water Storage ◽  
Acrylic Resin ◽  
Denture Base ◽  
Base Materials ◽  
Acrylic Resins ◽  
Group I ◽  
Significant Difference ◽  
Similar Cell

Introduction: The aim of this study was to evaluate the cytotoxic effect of various denture base materials following four different aging periods. Methods: In total, 48 disc-shaped specimens per each group were prepared: Group I: acrylic resin polymerized in cool water and heated up to 100°C over 45 min and boiled for 15 min; Group II: acrylic resin polymerized under pressure in 40°C–45°C water bath for 10 min; Group III: autopolymerized hard relining resin Cold Liner Rebase; Group IV: autopolymerized hard relining resin Truliner; Group V: soft relining resin DentuSil. Then the specimens were stored in water for 24 h or 15 days, or thermocycled 2500 times or 10,000 times. Cytotoxicity was evaluated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using L929 cells after 72-h cell incubation. Cell viability percentages were counted and statistical analyses were performed. The results were also evaluated according to ISO standard 10993-5. Results: All materials showed similar cell viability percentages following 24-h water storage and 2500 and 10,000 thermal cycles. Following 15-day water storage, a statistically significant difference was observed between the materials. Comparisons of the aging periods for each material showed statistically significant differences. Groups III and IV showed moderately cytotoxic effect following 15-day water storage. The remaining groups showed slightly cytotoxic or non-cytotoxic effect. Discussion: Polymerizing acrylic resins under pressure can be an alternative to conventional polymerizing to ensure a faster denture repair while providing similar cell viability values. Heat-cured acrylic resins provide higher cell viability than hard chairside lining materials in a 15-day period.

scholarly journals The effect of Zirconium Dioxide nanoparticles concentrations as filler on heat cured acrylic resin denture base toward viability of fibroblast cells (in vitro study)

2021 ◽  
Vol 6 (2) ◽  
pp. 71
Author(s):  
Rudy S ◽  
Titik Ismiyati ◽  
Endang Wahyuningtyas
Keyword(s):  
Cell Viability ◽  
Zirconium Dioxide ◽  
Acrylic Resin ◽  
Zro2 Nanoparticles ◽  
Fibroblast Cells ◽  
Denture Base ◽  
Group I ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Heat cured acrylic resin is the most commonly used denture base materials.  Zirconium dioxide (ZrO2) nanoparticles can be applied as additional filler to increase mechanical strength and to reduce the amount of residual monomer. The aim of this research is to analyze the effect of ZrO2 nanoparticles concentrations as filler on heat cured acrylic resin denture base toward viability of fibroblast cells. Twenty four disc-shaped heat cured acrylic resin plate (diameter 5 mm; width 2 mm), were divided into 4 groups (n=6), they were consisted of group I control (acrylic resin), group II acrylic resin with 2.5% ZrO2, group III acrylic resin with 5% ZrO2, and group IV acrylic resin with 7.5% ZrO2. Cell viability was obtained using MTT assay and ELISA plate reader. The result is examined with one way ANOVA followed by LSD post hoc assessment. The result showed highest cell viability percentage on experimental group of 2.5% ZrO2 with value as high as 97.49%. One way ANOVA test and LSD post hoc test showed a significant difference between groups (p<0.05). The conclusion of this research is ZrO2 nanoparticles concentration utilized as filler on heat cured acrylic resin denture base is effect to viability of fibroblast cells, and ZrO2 nanoparticles 2.5% shows the highest viability of fibroblast cell compared to 5% and 7.5% ZrO2 nanoparticles concentrations  

scholarly journals Tensile and transverse strength of novel copolymers for denture base.

2019 ◽  
Vol S (1) ◽  
pp. 7-10
Author(s):  
Ahmed Asim Saeed Al-Ali ◽  
◽  
Ammar k. Al-Noori ◽  
Amer A. Taqa ◽  
◽  
...  
Keyword(s):  
Acrylic Resin ◽  
P Value ◽  
Transverse Strain ◽  
The Novel ◽  
Denture Base ◽  
Transverse Strength ◽  
Base Materials ◽  
Propenoic Acid ◽  
Significant Difference ◽  
Transverse Strains

Objectives: Compare tensile and transverse strength of new copolymers for denture base. Materials and methods: The specimens were prepared from heat cured acrylic resin with three types of additives: Acryester B, Ethoxycarbonylethylene, and Propenoic acid at a percentage of 5% and 10%. The tensile and transverse strains were tested, recorded and compared. Results: The analysis of variance display statistically significant difference. The p-value was 0.001 for each of tensile and transverse strain tests. Conclusions: The tensile strength of the novel copolymers increased. The transverse strength of some of the novel copolymers increased.

Evaluation of Tissue Reaction to Some Denture-base Materials: An Animal Study

2008 ◽  
Vol 9 (4) ◽  
pp. 67-74 ◽  
Author(s):  
Behnaz Ebadian ◽  
Mohammad Razavi ◽  
Solmaz Soleimanpour ◽  
Ramin Mosharraf
Keyword(s):  
Animal Study ◽  
Weight Ratio ◽  
Acrylic Resin ◽  
Tissue Reaction ◽  
Control Group ◽  
Denture Base ◽  
Base Materials ◽  
Heat Cure ◽  
Two Samples ◽  
Significant Difference

Abstract Aim Controversy continues regarding the biocompatibility of denture base materials. One method to evaluate the biocompatibility of materials is in an animal study. Using dogs as subjects, the purpose of this study was to evaluate the vestibular tissue reaction to cobalt chromium (Co-Cr), heat cure acrylic resin, and acrylic resin mixed with aluminum oxide (Al2O3) compared with a control group using the histopathologic method. Methods and Materials Twelve disk shape samples (2 mm × 8 mm) in four groups of Co-Cr, acrylic resin, acrylic resin mixed with a 20% weight ratio of Al2O3, and a control group (Teflon) were fabricated. In one stage surgery two samples of each material (8 samples) was implanted in the buccal vestibule of each dog (n=6), subcutaneously. At 45 and 90-day intervals, half of the samples were excised along with peripheral tissue to assess the presence of inflammation by grading on a scale from 0 to 3 and the presence of a fibrotic capsule using histological observations. Data were analyzed using the Kruskal-Wallis, Mann-Whitney, and Tau b Kendal tests. Results Tissue reaction between Co-Cr and the control group was significant (P=0.02), but it was not significant between other groups. There was no significant difference between the 45 and 90-day postinsertion samples. The formation of fibrotic capsule groups was significant (P=0.01). It was significant between the Co-Cr and acrylic resin groups (P=0.01) and the acrylic resin and control groups (P=0.01). Conclusion The Co-Cr group was more toxic than the other groups. The inflammation increased during time. The inflammation in two acrylic groups was greater than the control and less than the Co-Cr group. The formation of fibrotic capsule, except in the acrylic resin with Al2O3 group, increased over time. Clinical Significance Co-Cr alloys are toxic and can produce damage to living tissue. Heat cure acrylic resin materials have less toxicity, and their use is safer than Co-Cr alloys. Citation Ebadian B, Razavi M, Soleimanpour S, Mosharraf R. Evaluation of Tissue Reaction to Some Denture-base Materials: An Animal Study. J Contemp Dent Pract 2008 May; (9)4:067-074.

scholarly journals Variations in Flexural Strength of Heat-polymerized Acrylic Resin after the Usage of Denture Cleansers

2016 ◽  
Vol 17 (4) ◽  
pp. 322-326 ◽  
Author(s):  
M Kalavathi ◽  
Mallikarjuna Ragher ◽  
G Vinayakumar ◽  
Sanketsopan Patil ◽  
Aishwarya Chatterjee ◽  
...  
Keyword(s):  
Flexural Strength ◽  
Testing Machine ◽  
Peak Load ◽  
Acrylic Resin ◽  
Control Group ◽  
Denture Base ◽  
Acrylic Resins ◽  
Significant Difference ◽  
Base Resin ◽  
Denture Cleansers

ABSTRACT Objective The objective of this study was to evaluate and compare changes in the flexural strength of heat-cured denture base resins when treated using denture cleansers. Study design A total of 40 specimens with dimension 65 mm length, 10 mm width, and 3 mm thickness were prepared as per ISO 1567 specification. A total of 10 specimens were immersed in distilled water to be used as control. Of the remaining 30 samples, 10 were treated with Clinsodent, 10 with VI-Clean, and 10 with Clanden denture cleansers. Specimens in each group were subjected to three-point flexural load in universal testing machine at a cross-head speed of 5 mm/min. The peak load (N) was recorded and flexural strength was calculated. The findings were analyzed using Kruskal–Wallis analysis of variance and Mann–Whitney test. Results Heat-cured denture base resin selected for this study showed significant difference in flexural strength after immersion in denture cleansers Clinsodent, VI-Clean, and Clanden solutions, when compared with the control group. Conclusion Findings of this study showed that denture cleansers altered the flexural strength of heat polymerized acrylic resins that endured soaking cycles which simulated 180 days of use. Hence, denture cleansers should be used with caution, once a day after brushing the dentures. It is advisable for patients to follow the manufacturer's instructions. How to cite this article Ragher M, Vinayakumar G, Patil S, Chatterjee A, Mallikarjuna DM, Dandekeri S, Swetha V, Pradeep MR. Variations in Flexural Strength of Heat-polymerized Acrylic Resin after the Usage of Denture Cleansers. J Contemp Dent Pract 2016;17(4):322-326.

scholarly journals Influence of aging on bond strength of artificial teeth to denture base acrylic resins

2018 ◽  
Vol 17 ◽  
pp. 1-9 ◽  
Author(s):  
Etiene Faria Aguiar ◽  
Rafaella Tonani ◽  
Fabiana de Goes Paiola ◽  
Michelle Alexandra Chinelatti ◽  
Carolina Noronha Ferraz de Arruda ◽  
...  
Keyword(s):  
Bond Strength ◽  
Acrylic Resin ◽  
Denture Base ◽  
Thermomechanical Cycling ◽  
Acrylic Resins ◽  
Significant Difference ◽  
Different Types ◽  
Base Resin ◽  
Artificial Teeth ◽  
Denture Base Resin

Aim: The aim of this study was to evaluate the bond strength of artificial teeth to different types of denture base resins when submitted to thermomechanical cycling (TMC). Methods: Sixty artificial mandibular first molars (Trilux, Vipi) were randomly divided into 3 groups according to denture base acrylic resins (Vipi Wave, Vipi Cril, and Vipi Cril Plus, Vipi). The teeth were fixed onto self-polymerizing acrylic resin bars (0.5 cm2 cross-section x 2 cm height), and the set was included in a metal flask using dental stone/silicone. After the dental stone was set, the bar was removed, and the denture base resin was packed and processed according to the group studied (Vipi Wave: 180 W/20 minutes + 540W/5 minutes; Vipi Cril and Vipi Cril Plus: Water bath at 74ºC for 9h). After polymerization, the samples were divided into 2 groups (n=10), according to the TMC treatment received (simulation of 5 years of mastication or not). The samples were submitted to tensile bond strength test (1 mm/min), and the data (MPa) were statistically analyzed (2-way ANOVA, Bonferroni, α=0.05). The fracture interfaces were evaluated using a stereomicroscope (50x). Results: The bond strength results showed no statistically significant difference (p>0.05) between the resins studied. TMC was significant (p<0.05), demonstrating lower values for the bond strength of artificial teeth to Vipi Cril Plus. The predominant fracture type was cohesive in resin. Conclusions: It was concluded that there is no difference in bond strength between artificial teeth and the resins used for denture base. However, TMC decreases the bond strength values of artificial teeth and crosslink thermo-polymerizable acrylic resin.

scholarly journals Effect of nano silica coating concentration on reducing acrylic resin denture cytotoxicity

2020 ◽  
Vol 5 (3) ◽  
pp. 126
Author(s):  
Gene Rizky Natalia Gunawan ◽  
Titik Ismiyati ◽  
Haryo Mustiko Dipoyono
Keyword(s):  
Cell Viability ◽  
Base Material ◽  
Acrylic Resin ◽  
Silica Coating ◽  
Control Group ◽  
Nano Silica ◽  
Residual Monomer ◽  
Denture Base ◽  
Significant Difference ◽  
Monomer Content

Acrylic resin is a denture base material mostly used in the fabrication of removable partial denture and complete denture because it is easy to process, economical and aesthetically satisfying. However, the acrylic resin has a weakness i.e. residual monomer content which causes cytotoxic effect to human cells. Nano-silica coating could be used on the acrylic resin to reduce residual monomer content. The purpose of this study was to determine the effect of nano-silica coating concentration on the cytotoxicity of acrylic resin denture base material. Twenty-four acrylic resin disc-shaped specimens (5 mm in diameter and 2 mm in thickness) were divided into 4 groups (n=6), i.e. control, group with application of 0.5%,1%, and 2% nano silica coating. The specimens on the silica coating groups were silica coated using dip-coating method. After the coating was completed, the specimens were added into cell fibroblast culture (vero cell line). Cytotoxicity testswere done by MTT assay. The optical density was measured using ELISA plate reader to determine the percentage of cell viability. The highest mean of cell viability was shown in 0.5% concentration (96.78 ± 1.71) and the lowest mean was shown in the control group (80.32 ± 6.53). One-way ANOVA showed that there were significant differences among these groups. LSD tests showed that there was significant difference among all of the groups. This study concluded that nano silica coating concentration has a significant effect on the cytotoxicity of acrylic resin denture base materials.

scholarly journals Hardness of denture base and hard chair-side reline acrylic resins

2005 ◽  
Vol 13 (3) ◽  
pp. 291-295 ◽  
Author(s):  
Andrea Azevedo ◽  
Ana Lucia Machado ◽  
Carlos Eduardo Vergani ◽  
Eunice Teresinha Giampaolo ◽  
Ana Cláudia Pavarina
Keyword(s):  
Surface Hardness ◽  
Acrylic Resin ◽  
Polymerization Reaction ◽  
Time Intervals ◽  
Denture Base ◽  
Continuous Increase ◽  
Hardness Tester ◽  
Dry Storage ◽  
Base Materials ◽  
Acrylic Resins

INTRODUCTION: The hardness of denture base materials may undergo changes due to continued polymerization reaction and water uptake. However, the extent to which these processes affect the hardness of materials is still unclear. OBJECTIVE: In this study, the degree of conversion of two hard chair-side reline resins (Duraliner II-D and Kooliner-K) and one heat-cured acrylic resin (Lucitone 550-L) was evaluated indirectly by measuring the surface hardness. The effect of immersion in water on this property was also analyzed. MATERIALS AND METHODS: After processing following the manufacturers' instructions, specimens (5mm diameter and 2mm thickness) were dry stored at room temperature and the Vickers hardness (VHN) was measured with a hardness tester after 0, 2, 7, 30 and 90 days. Specimens were then immersed in water at 37ºC and hardness was evaluated after the same time intervals. Five specimens were prepared for each material. Data were analyzed by Kruskal-Wallis test (P=.01). RESULTS: When dry stored, material L showed an increase in hardness (P<.01) from 0-day (VHN=23.2) to 30-day (VHN=27.1), after which no significant change was observed. A continuous increase in hardness was observed for material D from 0-day (VHN=4.6) to 90-day dry storage (VHN=7.3). For material K, an increase in hardness was observed up to 7-day (VHN=9.2), and then leveled off (P>.01). After 2-day water storage, all materials showed a significant reduction in hardness (P<.01). CONCLUSION: In general, the hardness of the materials evaluated increased during dry storage and decreased after immersion in water.

scholarly journals Effect of different palatal vault shapes on the dimensional stability of glass fiber-reinforced heat-polymerized acrylic resin denture base material

2012 ◽  
Vol 06 (01) ◽  
pp. 070-078 ◽  
Author(s):  
Mehmet Dalkiz ◽  
Demet Arslan ◽  
Riza Ali Tuncdemir ◽  
Selim M Bilgin ◽  
Halil Aykul
Keyword(s):  
Glass Fiber ◽  
Dimensional Stability ◽  
Water Storage ◽  
Base Material ◽  
Acrylic Resin ◽  
Reference Points ◽  
Denture Base ◽  
Glass Fiber Reinforced ◽  
Significant Difference ◽  
Palatal Vault

ABSTRACTObjective: The aim of this study was to determine the effect of different palatal vault shapes on the dimensional stability of a glass fiber reinforced heat polymerized acrylic resin denture base materialMethods: Three edentulous maxilla with shallow, deep and medium shaped palatal vaults were selected and elastomeric impressions were obtained. A maxillary cast with four reference points (A, B, C, and D) was prepared to serve as control. Point (A) was marked in the anterior midline of the edentulous ridge in the incisive papillary region, points (B) and (C) were marked in the right and left posterior midlines of the edentulous ridge in the second molar regions, and point (D) was marked in the posterior palatal midline near the fovea palatina media (Figure 2). To determine linear dimensional changes, distances between four reference points (A-B, A-C, A-D and B-C) were initially measured with a metal gauge accurate within 0.1 mm under a binocular stereo light microscope and data (mm) were recordedResults:No significant difference of interfacial distance was found in sagittal and frontal sections measured 24 h after polymerization and after 30 days of water storage in any of experimental groups (P>05). Significant difference of linear dimension were found in all experimental groups (P<.01) between measurements made 24 h after polymerization of specimens and 30 days after water storageConclusion: Palatal vault shape and fiber impregnation into the acrylic resin bases did not affect the magnitude of interfacial gaps between the bases and the stone cast surfaces. (Eur J Dent 2012;6:70-78)

scholarly journals The Influence of Saliva pH on the Fracture Resistance of Three Complete Denture Base Acrylic Resins

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Juliana de Sá ◽  
Francisca Vieira ◽  
Carlos Manuel Aroso ◽  
Mónica Cardoso ◽  
José Manuel Mendes ◽  
...  
Keyword(s):  
Fracture Resistance ◽  
Artificial Saliva ◽  
Testing Machine ◽  
Acrylic Resin ◽  
Low Ph ◽  
Data Sets ◽  
Denture Base ◽  
Base Materials ◽  
Acrylic Resins ◽  
Ph Conditions

Aim and Background. The absorption of fluids by denture base materials diminishes the mechanical properties such as hardness, strength, and fatigue limit due to the plasticizing effect of water. This study aimed to evaluate the influence of oral pH on the fracture resistance of prosthetic bases made of three different heat-polymerizable acrylic resin brands: RS Vertex®, Triplex Hot®, and Megacryl®. The strength needed to fracture the resins and the resistance to fracture each resin were evaluated. Methods. Ten prosthesis bases prepared with each brand of resin were subjected to neutral and low pH conditions (pH 7 and pH 4) by submerging them in artificial saliva for 30 days. After exposure, the fatigue resistance of the resins was tested using a Dental CS® Testing Machine. Statistical Analysis Test. The data sets were described quantitatively in terms of mean (M) and standard deviation (SD). Shapiro–Wilk tests and unilateral analysis of variance (ANOVA) were performed and complemented by Tukey’s multiple comparison tests. The effect size (η2), whose cohort points followed Cohen’s recommendations: 0.01 (low), 0.06 (medium), and 0.14 (high), was calculated. The results were considered significant if p < 0.05 and marginally significant if p < 0.10 . Results. One-way ANOVA showed that Megacryl® had the highest fracture resistance at pH 7 (52.23 Kgf), compared with Triplex Hot® ( p < 0.001 ) and RS Vertex® ( p = 0.034 ). Two-way ANOVA confirmed the interaction between brand and pH ( p = 0.022 ), also revealing that brands comparison is significant or marginally significant, when pH is not considered (Megacryl® versus Triplex Hot®, p < 0.001 , and RS Vertex®, p = 0.058 ; Triplex Hot® versus RS Vertex®, p = 0.051 ), and pH 7 results were significantly higher ( p = 0.003 ), even when brands are not considered. Hence, Megacryl® at pH 7 was found to have the highest fracture resistance, detached from other brands and pH values. Conclusion. It can be concluded within the limitations of this study that there are differences in the fracture resistance among the three brands of acrylic resin. Megacryl® was found to have the highest fracture resistance, and Triplex Hot® was the lowest. The results also show that exposure to a low pH environment decreases the fracture resistance of the Megacryl® and RS Vertex® resins.

scholarly journals Influence of storage period and effect of different brands of acrylic resin on the dimensional accuracy of the maxillary denture base

2008 ◽  
Vol 19 (3) ◽  
pp. 204-208 ◽  
Author(s):  
Ana Carolina Miéssi ◽  
Marcelo Coelho Goiato ◽  
Daniela Micheline dos Santos ◽  
Stefan Fiúza de Carvalho Dekon ◽  
Ricardo Coelho Okida
Keyword(s):  
Storage Period ◽  
Dimensional Accuracy ◽  
Acrylic Resin ◽  
Microwave Energy ◽  
Denture Base ◽  
Significance Level ◽  
Dimensional Changes ◽  
Acrylic Resins ◽  
Significant Difference ◽  
Hot Bath

The aim of this study was to evaluate the dimensional changes of denture bases made from different resins after different storage periods. For this purpose, 25 sets of plaster models/resin bases were prepared using 4 acrylic resins submitted to two types of polymerization: 1- QC-20 submitted to polymerization by microwave energy; 2- QC-20 submitted to polymerization by water hot bath; 3- Vipi Cril submitted to polymerization by water hot bath; 4- Vipi Wave submitted to polymerization by microwave energy; and 5- Onda Cryl submitted to polymerization by microwave energy. After polymerization, the specimens were sectioned for accuracy readings using a comparison microscope. Readings were taken at 3 points: the crests of the right (A) and left (B) ridges, and the median region of the palate, in 4 different periods. The data obtained were submitted to two-way ANOVA and Tukey's test at 5% significance level. The greatest distortions were found in the posterior palatal region of the base (M), with statistically significant difference (p<0.05) for the studied resins. All acrylic resins presented dimensional changes and the storage period influenced these alterations.

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