scholarly journals Effect of nano silica coating concentration on reducing acrylic resin denture cytotoxicity

2020 ◽  
Vol 5 (3) ◽  
pp. 126
Author(s):  
Gene Rizky Natalia Gunawan ◽  
Titik Ismiyati ◽  
Haryo Mustiko Dipoyono
Keyword(s):  
Cell Viability ◽  
Base Material ◽  
Acrylic Resin ◽  
Silica Coating ◽  
Control Group ◽  
Nano Silica ◽  
Residual Monomer ◽  
Denture Base ◽  
Significant Difference ◽  
Monomer Content

Acrylic resin is a denture base material mostly used in the fabrication of removable partial denture and complete denture because it is easy to process, economical and aesthetically satisfying. However, the acrylic resin has a weakness i.e. residual monomer content which causes cytotoxic effect to human cells. Nano-silica coating could be used on the acrylic resin to reduce residual monomer content. The purpose of this study was to determine the effect of nano-silica coating concentration on the cytotoxicity of acrylic resin denture base material. Twenty-four acrylic resin disc-shaped specimens (5 mm in diameter and 2 mm in thickness) were divided into 4 groups (n=6), i.e. control, group with application of 0.5%,1%, and 2% nano silica coating. The specimens on the silica coating groups were silica coated using dip-coating method. After the coating was completed, the specimens were added into cell fibroblast culture (vero cell line). Cytotoxicity testswere done by MTT assay. The optical density was measured using ELISA plate reader to determine the percentage of cell viability. The highest mean of cell viability was shown in 0.5% concentration (96.78 ± 1.71) and the lowest mean was shown in the control group (80.32 ± 6.53). One-way ANOVA showed that there were significant differences among these groups. LSD tests showed that there was significant difference among all of the groups. This study concluded that nano silica coating concentration has a significant effect on the cytotoxicity of acrylic resin denture base materials.

scholarly journals Evaluation of some properties of recycled polymethylmethacrylate incorporated to the acrylic resin

2020 ◽  
Vol 11 (3) ◽  
pp. 2765-2771
Author(s):  
Amrah Y. Al Jmmal ◽  
Nada Z. Mohammed ◽  
Amer A. Taqa
Keyword(s):  
Water Sorption ◽  
Surface Hardness ◽  
Base Material ◽  
Acrylic Resin ◽  
Control Group ◽  
Residual Monomer ◽  
Denture Base ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Many attempts have been made enhancing properties of PMMA denture base material. Adding fillers to PMMA was a commonly used method to improve physical and mechanical properties. To estimate the effects of incorporating recycled polymethylmethacrylate with a different percentage with conventional heat-cured acrylic resin on some properties of heat-cured denture base resin. The recycled polymethylmethacrylate (Chaini-HK G) particles dissolved at (5%,10%,15%) was added to heat-cured polymethylmethacrylate (Spofadental, Czech Republic) polymer and mixed with a ratio of 2:1 with the polymethylmethacrylate monomer. A total number of specimens were 80, which divided into three experimental groups (5%, 10%, 15%) and control group. Each group have 20specimens. The surface hardness, water sorption, solubility and residual monomer of test groups measured as well as compared to that of control groups. Fourier transform infrared spectroscopy test (FTIR) was done for three experimental and control group. This study shows a statistically significant difference at (p<0.01) in the hardness of recycled polymethylmethacrylate incorporate material (10 %,15%) groups when compared with a control group; while recycled polymethylmethacrylate incorporated (5%) group show no significant difference, group with 15% shows the highest Vickers hardness value. The mean values of water sorption test solubility test and residual monomer were decreased with the increase of incorporating a percentage of recycled polymethylmethacrylate as compared to the mean value of the control group. The incorporation of recycled polymethylmethacrylate to heat acrylic resin at different percentage improves some of its properties.

scholarly journals Effect of addition titanium dioxide nanoparticles as acrylic resin denture base filler on cytotoxicity

2020 ◽  
Vol 5 (2) ◽  
pp. 86
Author(s):  
Ardhianing Hardita ◽  
Titik Ismiyati ◽  
Endang Wahyuningtyas
Keyword(s):  
Titanium Dioxide ◽  
Treatment Group ◽  
Cell Viability ◽  
Tio2 Nanoparticles ◽  
Base Material ◽  
Acrylic Resin ◽  
Control Group ◽  
Fibroblast Cells ◽  
Residual Monomer ◽  
Denture Base

Denture base material should have a good level of biocompatibility. Acrylic resin is frequently used as a denture base material, however it has a disadvantage of producing residual monomer. Residual monomer is known to have a cytotoxicity effect. Titanium dioxide (TiO2) nanoparticles are used as fillers due to their biocompatibility and ability to enhance the mechanical properties of acrylic resin. The addition of the material to acrylic resin could affect the amount of residual monomer. The aim of this study was to examine the effect of the addition of TiO2 nanoparticles as acrylic resin denture base filler on the cytotoxicity in fibroblast cells. The samples consisted of 24 heat cured acrylic resins in disc shape (5 mm in diameter and 2 mm in thickness), divided into 4 groups (n = 6): three groups given treatment with0.5%, 1%, 2% TiO2, respectively and one control group. Cell viability was measured with MTT assay. The results were tested with one way ANOVA with 95% confidence level followed by LSD post hoc test. The results showed that the highest percentage of cell viability was found in the treatment group of 0.5% TiO2 with value of 91.83 ± 1.75%, while the lowest value was seen in the treatment group of 2% TiO2 with value of 79.38 ± 3.34%. Significant differences were shown between the treatment groups of 0.5% and 2% TiO2, as well as between the control and treatment group with 2% TiO2. The conclusions of this research are the addition of TiO2 nanoparticles as acrylic resin denture base filler has an effect on cytotoxicity; the addition of 0.5% TiO2 nanoparticles filler results in lower cytotoxicity on fibroblast cells compared to the addition of 1% and 2% TiO2.  

scholarly journals The effect of nanoparticles TiO2 on the flexural strength of acrylic resin denture plate

2018 ◽  
Vol 30 (1) ◽  
pp. 35 ◽  
Author(s):  
Edwin Tandra ◽  
Endang Wahyuningtyas ◽  
Erwan Sugiatno
Keyword(s):  
Flexural Strength ◽  
Testing Machine ◽  
Base Material ◽  
Acrylic Resin ◽  
Control Group ◽  
Denture Base ◽  
Denture Plate ◽  
Significant Difference ◽  
Strength Difference ◽  
The One

Introduction: Acrylic resin is still the most commonly used denture base material due to its ideal properties. However, acrylic resin denture fractures are still considered a major unsolved problem thus the addition of nanoparticles as filler was performed to increase its mechanical properties. The purpose of this study was to discovered the effect of nanoparticles TiO2 on the flexural strength of acrylic resin denture plate. Method: This study used 27 heat-cured acrylic resin specimens sized 65 x 10 x 2.5 mm. The samples were divided into three concentration groups (n = 9), the control group; 1% of nanoparticles TiO2; and 3% of nanoparticles TiO2. The flexural strength was tested using the Universal Testing Machine. All data were analysed using the one-way ANOVA test with 95% confidence level then continued with the Least Significant Difference (LSD) test. Results: There were significant flexural strength differences in different concentration of nanoparticles TiO2. The highest flexural strength value was found in the 1% of nanoparticles TiO2 group (106.99 ± 6.09 MPa), whilst the lowest flexural strength value was found in the 3% of nanoparticles TiO2 group (91.64 ± 5.38 MPa). Significant flexural strength difference was found between the control group and the 1% of nanoparticles TiO2 group, and also between the 1% of nanoparticles TiO2 group with the 3% of nanoparticles TiO2 group (p < 0.05). Conclusion: From this study can be concluded that concentration of 1% of nanoparticles TiO2 was able to increase the flexural strength of acrylic resin denture plate.

scholarly journals The Effect of Alcoholic Beverages on Surface Roughness of Acrylic Denture Base Material

2020 ◽  
Vol 5 (1) ◽  
pp. 36-43
Author(s):  
Gladis Aprilla Rizki ◽  
Sri Wahyuningsih Rais ◽  
Martha Mozartha
Keyword(s):  
Surface Roughness ◽  
Artificial Saliva ◽  
Alcoholic Beverage ◽  
Base Material ◽  
Acrylic Resin ◽  
Alcoholic Beverages ◽  
Control Group ◽  
Test Surface ◽  
Denture Base ◽  
Significant Difference

Alcoholic beverages can be consumed in any group age, and it may be consumed by an individual using denture. Fluid absorption by heat-cured acrylic resin as a material of a denture base was able to cause an increase in its surface roughness. Absorption of an organic solvent such as the ethanol in an alcoholic beverage(s) could even increase the effect. This study aimed to evaluate the effect of alcohol beverages on surface roughness of heat-cured acrylic resin denture base. Samples (n=24) were prepared from heat-cured acrylic resin (25x25x5 mm). They were divided into 4 groups and the pre-test surface roughness was measured. Samples of each group were immersed for 10 minutes into the solution (distilled water as the control group, beer, wine, and whiskey as the test groups). After stored in artificial saliva for 23 hours, the samples were immersed into the solution, completing a period of 24 hours. This procedure was performed for 7 consecutive days and the post-test surface roughness was measured. Data were statistically analyzed using the Kruskal-Walis test. There was a significant difference in surface roughness measurement before and after the immersion (p<0.05). Whiskey was most affecting the surface roughness of heat-cured acrylic resin denture base. Alcoholic beverages could increase the surface roughness of heat cure acrylic resin denture base. Keywords: Alcoholic beverages; Heat-cured acrylic denture base; Surface roughness

IMMERSION OF HEAT POLYMERIZED ACRYLIC RESIN DENTAL BASE IN ARAK BALI AGAINST SURFACE ROUGHNESS

2021 ◽  
Vol 17 (1) ◽  
pp. 22-26
Author(s):  
Kadek Ayu Wirayuni ◽  
◽  
I Made Hendri Dwi Saputra ◽  
Keyword(s):  
Surface Roughness ◽  
Acrylic Resin ◽  
Control Group ◽  
Surface Strength ◽  
Denture Base ◽  
A Value ◽  
Removable Denture ◽  
Significant Difference ◽  
Post Test ◽  
Oral Tissue

Introduction: The denture base is the part of the removable denture that is supported by good adaptation to the underlying oral tissue. Most of the denture bases are made of acrylic or polymethyl methacrylate resin, better known as PMMA. However, the acrylic resin also has disadvantages such as easily broken and absorbs liquids both water and chemicals. The chemical absorption like alcohol, ethanol, and some drinks that contain acidic materials will chemically be induced with acrylic resin and settle in the pores of the acrylic resin. The chemical damage or defect creates roughness on the surface of the acrylic resin which can cause cracking or crazing and a decrease in surface strength and hardness. Materials and Methods: The method used in this research was a laboratory experimental design with a post-test-only control group using 12 samples consisting of 2 different types of samples by measuring the surface roughness of the acrylic resin after immersing the sample with a predetermined time. Results and Discussions: One-way ANOVA test results showed a significant difference in surface roughness after the samples immersion with a value of p = 0.006 (p <0.05). Conclusions: Based on this research, can be concluded that there is an increase in the surface roughness of the heated polymerized acrylic resin for 3 hours and 4 hours of immersion. The longer the heated polymerized acrylic resin is soaked in arak hence the level of surface roughness increases.

scholarly journals Denture Acrylic Resin Material with Antibacterial and Protein-Repelling Properties for the Prevention of Denture Stomatitis

Polymers ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 230
Author(s):  
Salwa Omar Bajunaid ◽  
Bashayer H. Baras ◽  
Michael D. Weir ◽  
Hockin H. K. Xu
Keyword(s):  
Oral Mucosa ◽  
Pathological Condition ◽  
Base Material ◽  
Acrylic Resin ◽  
Control Group ◽  
Control Groups ◽  
Denture Base ◽  
Denture Stomatitis ◽  
Colony Forming Unit ◽  
Bioactive Agents

Denture stomatitis is a multifactorial pathological condition of the oral mucosa that affects up to 72% of denture wearers. It is commonly seen on the palatal mucosa and characterized by erythema on the oral mucosa that are in contact with the denture surface. The aim of this study was to incorporate 2-methacryloyloxyethyl phosphorylcholine (MPC) and dimethylaminohexadecyl methacrylate (DMAHDM) into a high impact polymethylmethacrylate heat-cured denture base acrylic resin as a potential treatment for denture stomatitis. We used a comparative study design to examine the effect of incorporating MPC as a protein repellent agent and DMAHDM as an antifungal agent to prevent the adherence of Candida albicans to the denture base material. The dual incorporation of MPC and DMAHDM reduced C. albicans biofilm colony-forming unit by two orders of magnitude when compared to the control group devoid of the bioactive agents. Although the addition of MPC and DMAHDM alone or in combination significantly reduced the flexural strength of the material, they showed reduced roughness values when compared to control groups. This new denture acrylic resin provides the benefit of enhancing C. albicans biofilm elimination through dual mechanisms of action, which could potentially reduce the prevalence of denture stomatitis.

Evaluation of Tissue Reaction to Some Denture-base Materials: An Animal Study

2008 ◽  
Vol 9 (4) ◽  
pp. 67-74 ◽  
Author(s):  
Behnaz Ebadian ◽  
Mohammad Razavi ◽  
Solmaz Soleimanpour ◽  
Ramin Mosharraf
Keyword(s):  
Animal Study ◽  
Weight Ratio ◽  
Acrylic Resin ◽  
Tissue Reaction ◽  
Control Group ◽  
Denture Base ◽  
Base Materials ◽  
Heat Cure ◽  
Two Samples ◽  
Significant Difference

Abstract Aim Controversy continues regarding the biocompatibility of denture base materials. One method to evaluate the biocompatibility of materials is in an animal study. Using dogs as subjects, the purpose of this study was to evaluate the vestibular tissue reaction to cobalt chromium (Co-Cr), heat cure acrylic resin, and acrylic resin mixed with aluminum oxide (Al2O3) compared with a control group using the histopathologic method. Methods and Materials Twelve disk shape samples (2 mm × 8 mm) in four groups of Co-Cr, acrylic resin, acrylic resin mixed with a 20% weight ratio of Al2O3, and a control group (Teflon) were fabricated. In one stage surgery two samples of each material (8 samples) was implanted in the buccal vestibule of each dog (n=6), subcutaneously. At 45 and 90-day intervals, half of the samples were excised along with peripheral tissue to assess the presence of inflammation by grading on a scale from 0 to 3 and the presence of a fibrotic capsule using histological observations. Data were analyzed using the Kruskal-Wallis, Mann-Whitney, and Tau b Kendal tests. Results Tissue reaction between Co-Cr and the control group was significant (P=0.02), but it was not significant between other groups. There was no significant difference between the 45 and 90-day postinsertion samples. The formation of fibrotic capsule groups was significant (P=0.01). It was significant between the Co-Cr and acrylic resin groups (P=0.01) and the acrylic resin and control groups (P=0.01). Conclusion The Co-Cr group was more toxic than the other groups. The inflammation increased during time. The inflammation in two acrylic groups was greater than the control and less than the Co-Cr group. The formation of fibrotic capsule, except in the acrylic resin with Al2O3 group, increased over time. Clinical Significance Co-Cr alloys are toxic and can produce damage to living tissue. Heat cure acrylic resin materials have less toxicity, and their use is safer than Co-Cr alloys. Citation Ebadian B, Razavi M, Soleimanpour S, Mosharraf R. Evaluation of Tissue Reaction to Some Denture-base Materials: An Animal Study. J Contemp Dent Pract 2008 May; (9)4:067-074.

scholarly journals The effect of polyamide microparticles addition on some mechanical prop-erties of light-cured acrylic resin

2019 ◽  
Vol 10 (2) ◽  
pp. 1464-1469
Author(s):  
Adnan R. Al Assal ◽  
Abdalbaset A Fatalla ◽  
Mohammed Moudhaffar ◽  
Ghasak H Jani
Keyword(s):  
Tensile Strength ◽  
Impact Strength ◽  
Base Material ◽  
Acrylic Resin ◽  
Strength Test ◽  
Control Group ◽  
Denture Base ◽  
Mean Values ◽  
Transverse Strength ◽  
Transverse Tensile

The general upgrading of polymer denture base material and research continuously looking for ideal restorative dental material with better properties, adequate esthetic properties, less expensive and easier to handle material to develop photo polymerization dental materials. This study was conducted to evaluate the effect of addition polyamide on mechanical microparticle properties light cure denture base material. One hindered sixty specimens from light-cured acrylic resin (Aurora). The divided mainly into four groups according to test used (Transverse strength test, impact strength test, hardness test and tensile strength test) with 40 specimens for each group. The results show an increase in Transverse strength, impact strength, hardness and tensile strength in all experimental group when compared to control group the highest mean values for all tests included in the study appeared in group B 1% polyamide. The addition of polyamide microparticle improves transverse, tensile, impact strength and hardness properties of denture base material.

scholarly journals The effect of Zirconium Dioxide nanoparticles concentrations as filler on heat cured acrylic resin denture base toward viability of fibroblast cells (in vitro study)

2021 ◽  
Vol 6 (2) ◽  
pp. 71
Author(s):  
Rudy S ◽  
Titik Ismiyati ◽  
Endang Wahyuningtyas
Keyword(s):  
Cell Viability ◽  
Zirconium Dioxide ◽  
Acrylic Resin ◽  
Zro2 Nanoparticles ◽  
Fibroblast Cells ◽  
Denture Base ◽  
Group I ◽  
Significant Difference ◽  
Post Hoc ◽  
One Way Anova

Heat cured acrylic resin is the most commonly used denture base materials.  Zirconium dioxide (ZrO2) nanoparticles can be applied as additional filler to increase mechanical strength and to reduce the amount of residual monomer. The aim of this research is to analyze the effect of ZrO2 nanoparticles concentrations as filler on heat cured acrylic resin denture base toward viability of fibroblast cells. Twenty four disc-shaped heat cured acrylic resin plate (diameter 5 mm; width 2 mm), were divided into 4 groups (n=6), they were consisted of group I control (acrylic resin), group II acrylic resin with 2.5% ZrO2, group III acrylic resin with 5% ZrO2, and group IV acrylic resin with 7.5% ZrO2. Cell viability was obtained using MTT assay and ELISA plate reader. The result is examined with one way ANOVA followed by LSD post hoc assessment. The result showed highest cell viability percentage on experimental group of 2.5% ZrO2 with value as high as 97.49%. One way ANOVA test and LSD post hoc test showed a significant difference between groups (p<0.05). The conclusion of this research is ZrO2 nanoparticles concentration utilized as filler on heat cured acrylic resin denture base is effect to viability of fibroblast cells, and ZrO2 nanoparticles 2.5% shows the highest viability of fibroblast cell compared to 5% and 7.5% ZrO2 nanoparticles concentrations  

Cytotoxicity of long-term denture base materials

2018 ◽  
Vol 41 (10) ◽  
pp. 677-683 ◽  
Author(s):  
Merve Çakırbay Tanış ◽  
Canan Akay ◽  
Handan Sevim
Keyword(s):  
Cell Viability ◽  
Cytotoxic Effect ◽  
Water Storage ◽  
Acrylic Resin ◽  
Denture Base ◽  
Base Materials ◽  
Acrylic Resins ◽  
Group I ◽  
Significant Difference ◽  
Similar Cell

Introduction: The aim of this study was to evaluate the cytotoxic effect of various denture base materials following four different aging periods. Methods: In total, 48 disc-shaped specimens per each group were prepared: Group I: acrylic resin polymerized in cool water and heated up to 100°C over 45 min and boiled for 15 min; Group II: acrylic resin polymerized under pressure in 40°C–45°C water bath for 10 min; Group III: autopolymerized hard relining resin Cold Liner Rebase; Group IV: autopolymerized hard relining resin Truliner; Group V: soft relining resin DentuSil. Then the specimens were stored in water for 24 h or 15 days, or thermocycled 2500 times or 10,000 times. Cytotoxicity was evaluated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using L929 cells after 72-h cell incubation. Cell viability percentages were counted and statistical analyses were performed. The results were also evaluated according to ISO standard 10993-5. Results: All materials showed similar cell viability percentages following 24-h water storage and 2500 and 10,000 thermal cycles. Following 15-day water storage, a statistically significant difference was observed between the materials. Comparisons of the aging periods for each material showed statistically significant differences. Groups III and IV showed moderately cytotoxic effect following 15-day water storage. The remaining groups showed slightly cytotoxic or non-cytotoxic effect. Discussion: Polymerizing acrylic resins under pressure can be an alternative to conventional polymerizing to ensure a faster denture repair while providing similar cell viability values. Heat-cured acrylic resins provide higher cell viability than hard chairside lining materials in a 15-day period.

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