α-Tocopherol (vitamin-E) ameliorates ferric nitrilotriacetate (Fe-NTA)-dependent renal proliferative response and toxicity: diminution of oxidative stress

1998 ◽  
Vol 17 (3) ◽  
pp. 163-171 ◽  
Author(s):  
Mohammad Iqbal ◽  
Hassan Rezazadeh ◽  
Sabah Ansar ◽  
Mohammad Athar
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Vitamin E ◽  
Serum Creatinine ◽  
Blood Urea Nitrogen ◽  
Thymidine Incorporation ◽  
Ferric Nitrilotriacetate ◽  
Hydrogen Peroxide Generation

Ferric nitrilotriacetate (Fe-NTA) is a potent nephrotoxic agent. In this communication, we show the modulatory effect of DL-a-tocopherol (Vitamin-E) on ferric nitrilotriacetate (Fe-NTA)-induced renal oxidative stress, toxicity and hyperproliferative response in rats. Fe-NTA-treatment enhances the susceptibility of renal microsomal membrane for iron-ascorbate-induced lipid peroxidation and hydrogen peroxide generation which are accompanied by a decrease in the activities of renal antioxidant enzymes, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase and depletion in the level of renal glutathione. Parallel to these changes, a sharp increase in blood urea nitrogen and serum creatinine has been observed. In addition, Fe-NTA-treatment also enhances renal ornithine decarboxylase activity (ODC) and increases [3H]thymidine incorporation in renal DNA. Prophylactic treatment of animals with Vit.E daily for 1 week prior to the administration of Fe-NTA resulted in the diminution of Fe-NTA-mediated damage. Enhanced susceptibility of renal microsomal membrane for lipid peroxidation induced by iron-ascorbate and hydrogen peroxide generation were significantly reduced (P50.05). In addition, the depleted level of glutathione and inhibited activities of antioxidant enzymes recovered to significant levels (P50.05). Similarly, the enhanced blood urea nitrogen and serum creatinine levels which are indicative of renal injury showed a reduction of about 50% at a higher dose of Vit.E. The pretreatment of rats with Vit.E reduced the Fe-NTA-mediated induction in ODC activity and enhancement in [3H]thymidine incorporation in DNA. The protective effect of Vit.E was dose dependent. In summary, our data suggest that Vit.E is an effective chemopreventive agent in kidney and may suppress Fe-NTA-induced renal toxicity.

Butylated hydroxyanisole alleviates ferric nitrilotriacetate induced nephrotoxicity in rats by abrogation of oxidative stress

2013 ◽  
Vol 3 (2) ◽  
pp. 65-70
Author(s):  
Sabah Ansar ◽  
Mohammad Iqbal ◽  
Noura Al Jameil
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Body Weight ◽  
Antioxidant Enzymes ◽  
Control Group ◽  
Butylated Hydroxyanisole ◽  
Chemopreventive Agent ◽  
Microsomal Lipid Peroxidation ◽  
Hydrogen Peroxide Generation

In this study the effect of butylated hydroxyanisole (BHA), a phenolic antioxidantused in food on Ferric‐Nitrilotriacetate (Fe–NTA) induced nephrotoxicity is reported. Fe‐NTA (9 mg Fe/kg body weight, intraperitoneally) treatment enhanced the renal microsomal lipid peroxidation and hydrogen peroxide generation to ~2‐2.5 folds compared to saline‐treated control and glutathione levels and the activities of antioxidant enzymes decreased to a range of 2–2.5 fold in kidney. These changes were reversed significantly in animals receiving a pretreatment of BHA. Pretreatment with BHA prior to Fe‐ NTA treatment reduced microsomal lipid peroxidation and hydrogen peroxide generation to 1.3‐1.5 fold compared to control group and glutathione and the activities of antioxidant enzymes increased to a range of 1.5‐2 folds in kidney. Fe‐NTA administration enhanced value of blood urea nitrogen and creatinine to 3.7 and 2.5 fold respectively as compared to their corresponding control group. Administration of Fe‐NTA to rats receiving a pretreatment of BHA led to a significant diminution in both of these values. The results indicate that BHA is a potent chemopreventive agent and suppresses Fe‐NTA induced nephrotoxicity in rats.

Nigella sativa (black cumin) ameliorates potassium bromate-induced early events of carcinogenesis: diminution of oxidative stress

2003 ◽  
Vol 22 (4) ◽  
pp. 193-203 ◽  
Author(s):  
Naghma Khan ◽  
Sonia Sharma ◽  
Sarwat Sultana
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Body Weight ◽  
Antioxidant Enzymes ◽  
Serum Creatinine ◽  
Xanthine Oxidase ◽  
Blood Urea Nitrogen ◽  
Nigella Sativa ◽  
Black Cumin ◽  
Glutamyl Transpeptidase

Potassium bromate (KBrO3) is a potent nephrotoxic agent. In this paper, we report the chemopreventive effect ofNigella sativa (black cumin) on KBrO3-mediated renal oxidative stress, toxicity and tumor promotion response in rats. KBrO3 (125 mg/kg body weight, intraperitoneally) enhances lipid peroxidation, g-glutamyl transpeptidase, hydrogen peroxide and xanthine oxidase with reduction in the activities of renal antioxidant enzymes and renal glutathione content. A marked increase in blood urea nitrogen and serum creatinine has also been observed. KBrO3 treatment also enhances ornithine decarboxylase (ODC) activity and [3H] thymidine incorporation into renal DNA. Prophylaxis of rats orally with Nigella sativaextract (50 mg/kg body weight and 100 mg/kg body weight) resulted in a significant decrease in renal microsomal lipid peroxidation (P B-00.001), g-glutamyl transpeptidase (P B-0.001), H2O2 (P B-0.001) and xanthine oxidase (P B-0.05). There was significant recovery of renal glutathione content (P B-0.01) and antioxidant enzymes (P B-0.001). There was also reversal in the enhancement of blood urea nitrogen, serum creatinine, renal ODC activity and DNA synthesis (P B-0.001). Data suggest that Nigella sativa is a potent chemopreventive agent and may suppress KBrO3-mediated renal oxidative stress, toxicity and tumour promotion response in rats.

Safety of Antitheilerial Drug Buparvaquone in Rams

2018 ◽  
Vol 46 (1) ◽  
Author(s):  
Nermin Isik ◽  
Ozlem Derinbay Ekici ◽  
Ceylan Ilhan ◽  
Devran Coskun
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Alkaline Phosphatase ◽  
Blood Cell ◽  
Blood Urea Nitrogen ◽  
Troponin I ◽  
Oxidative Status ◽  
Blood Samples ◽  
Heart Damage

 Background: Theileriosis is a tick-borne disease caused by Theileria strains of the protozoan species. Buparvaquone is the mostly preferred drug in the treatment theileriosis, while it is safety in sheep, has not been detailed investigated. It has been hypothesized that buparvaquone may show side effects and these effects may be defined some parameters measured from blood in sheep when it is used at the recommended dose and duration. The aim of this research was to determine the effect of buparvaquone on the blood oxidative status, cardiac, hepatic and renal damage and bone marrow function markers.Materials, Methods & Results: In this study, ten adult (> 2 years) Akkaraman rams were used. Healthy rams were placed in paddocks, provided water ad libitum, and fed with appropriate rations during the experiment. Buparvaquone was ad­ministered at the dose of 2.5 mg/kg (IM) intramuscularly twice at 3-day intervals. Blood samples were obtained before (0. h, Control) and after drug administration at 0.25, 0.5, 1, 2, 3, 4 and 5 days. The blood samples were transferred to gel tubes, and the sera were removed (2000 g, 15 min). During the study, the heart rate, respiratory rate, and body temperature were measured at each sampling time. In addition, the animals were clinically observed. Plasma oxidative status mark­ers (Malondialdehyde, total antioxidant status, catalase, glutathione peroxidase, superoxide dismutase), serum cardiac (Troponin I, creatine kinase-MBmass, lactate dehydrogenase), hepatic (Alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, gamma glutamyltransferase, total protein, albumin, globulin) and renal (Creatinine, blood urea nitrogen) damage markers and hemogram values (white blood cell, red blood cell, platelet, hemogram, hematocrit) were measured. Buparvaquone caused statistically significantly (P < 0.05) increases in the troponin I and blood urea nitrogen levels and fluctuations in alkaline phosphatase activity, but there was no any statistically significance difference determined in the other parameters.Discussion: In this study, buparvaquone was administered two times at a dose of 2.5 mg/kg (IM) at 3-day intervals. Al­though the result was not statistically significant (P > 0.05), it was determined that buparvaquone gradually increased the levels of the main oxidative stress marker, MDA, by approximately 2.8 fold. CAT and GPX levels were also found to have decreased by 2.2 fold. Buparvaquone may cause lipid peroxidation by producing free radicals. Some other antiprotozoal drugs may affect the oxidative status and may increase MDA level and decrease SOD level. In this study, MDA, which is an indicator of lipid peroxidation in vivo, was used to partially detect developing lipid peroxidation. Changes in the levels of reduced GPX and CAT enzymes could be attributed to their use in mediating the hydrogen peroxide detoxification mechanisms. The absence of significant changes in the TAS levels in this study suggests that buparvaquone may partially induce oxidative stress by producing hydrogen peroxide, but no significant changes occurred in the oxidative stress level because of the high antioxidant capacity of sheep. In this study, buparvaquone caused a statistically significant increase (P < 0.05) in the level of Tn-I, which is a marker of specific cardiac damage (P < 0.05), whereas there was no statistically (P > 0.05) significant increase in CK-MBmass. Tn-I and CK-MB levels, which are used to define heart damage in humans, have been successfully used to determine heart damage in sheep. In this research study, the statistically significant increases in Tn-I but not CK-MBmass levels could be considered indicative of mild cardiac damage.Keywords: ram, buparvaquone, safety.

scholarly journals The Effect of Nitrogen Fertilization and Fusarium culmorum Inoculation on the Biomarkers of Oxidative Stress in Wheat Flag Leaves

Poljoprivreda ◽  
2021 ◽  
Vol 27 (2) ◽  
pp. 15-24
Author(s):  
Magdalena Matić ◽  
◽  
Rosemary Vuković ◽  
Karolina Vrandečić ◽  
Ivna Štolfa Čamagajevac ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Biotic Stress ◽  
Flag Leaf ◽  
Fusarium Culmorum ◽  
Antioxidant Response ◽  
The Impact ◽  
Biomarkers Of Oxidative Stress

During cultivation, wheat is exposed to several abiotic and/or biotic stress conditions that may adversely impact the wheat yield and quality. The impact of abiotic stress caused by nitrogen deficiency and biotic stress caused by phytopathogenic fungus Fusarium culmorum on biomarkers of oxidative stress in the flag leaf of nine winter wheat varieties (Ficko, U-1, Galloper, BC Mandica, BC Opsesija, Ingenio, Isengrain, Felix, and Bezostaya-1) was analyzed in this study. Hydrogen peroxide concentration and lipid peroxidation level were measured as indicators of oxidative stress, while the antioxidant response was determined by measuring the concentration of phenolic compounds and activities of antioxidant enzymes. Wheat variety and nitrogen treatment had a significant effect on all examined biomarkers of oxidative stress in the flag leaf, while the impact of Fusarium treatment was less pronounced. The most significant impact on the measured stress biomarkers had a low nitrogen level, which mainly increased hydrogen peroxide concentration and lipid peroxidation level and decreased activities of antioxidant enzymes in most varieties. The obtained results were discussed and compared with the previous study in which biochemical analyzes were performed on the wheat spike. There was no significant strong correlation between flag leaf and spike response in the measured parameters, which, in addition to the variety-specific response, also indicates a tissue-specific antioxidant response.

Oxidative stress and antioxidant enzymes in triticale shoots under chloride salinization

2020 ◽  
Vol 63 (7) ◽  
pp. 99-105
Author(s):  
Viktor V. Ivanishchev ◽  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Cluster Analysis ◽  
Antioxidant Enzymes ◽  
Glutathione Reductase ◽  
Guaiacol Peroxidase ◽  
Short Term ◽  
Stress Indicators ◽  
Oxidative Stress Indicators

We studied alterations in oxidative stress indicators (hydrogen peroxide, superoxide radical, lipid peroxidation – LPO) and alterations in the activity of antioxidant enzymes (catalase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase) in triticale shoots (Triticosecale) during short-term (0-96 h) sodium chloride stress (120 mM) with statistical methods: principal component analysis (PCA) and cluster analysis. Analysis of alterations in the activity of enzymes with the PCA method does not allow them to be unambiguously included in a single group, despite the fact that they all belong to antioxidant enzymes. The inclusion of oxidative stress indicators in this analysis did not make the picture simpler. Using the cluster analysis method, it can be concluded that under conditions of short-term chloride stress in the shoots of triticale, much more catalase (than other enzymes studied) is associated with the protection of membranes from lipid peroxidation than with the utilization of hydrogen peroxide. This is also reflected by the highest correlation coefficients: catalase – LPO (0.94), catalase – hydrogen peroxide (0.79). The formation of primary clusters between ascorbate peroxidase and glutathione reductase reflect the significance of the association of the ascorbate – glutathione cycle with the processes of utilization of reactive oxygen species (primarily hydrogen peroxide) under experimental conditions. It was also shown that under conditions of short-term chloride stress in the shoots of triticale, guaiacol peroxidase plays the least role in the utilization of hydrogen peroxide. In this case, salt ions again form a single primary cluster, which combines with other clusters at the maximum Euclidean distance in the experiment.

Antioxidant and nephroprotective potential of butylated hydroxyanisole against ferric nitrilotriacetate-induced oxidative stress and early tumor events

2015 ◽  
Vol 35 (4) ◽  
pp. 448-453 ◽  
Author(s):  
S Ansar ◽  
M Iqbal
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Wistar Rats ◽  
Thymidine Incorporation ◽  
Albino Rats ◽  
Butylated Hydroxyanisole ◽  
Ferric Nitrilotriacetate ◽  
Wistar Strain ◽  
Male Wistar Rats ◽  
Early Tumor

The present study was aimed to study protective effect of butylated hydroxyanisole (BHA), a phenolic antioxidant used in foods on ferric nitrilotriacetate (Fe-NTA)–induced nephrotoxicity. Male albino rats of Wistar strain (4–6 weeks old) weighing 125–150 g were used in this study. Animals were given a single dose of Fe-NTA (9 mg kg−1 body weight) after treatment with BHA (1 and 2 mg animal−1 day−1). Fe-NTA treatment enhanced ornithine decarboxylase (ODC) activity to 5.3-fold, and [3H]-thymidine incorporation in DNA to 2.5-fold in kidney compared with the corresponding saline-treated control, whereas glutathione (GSH) levels and the activities of antioxidant enzymes decreased to a range of 2- to 2.5-fold in kidney. These changes were reversed significantly in animals receiving a pretreatment of BHA. The enhanced ODC activity and DNA synthesis showed a reduction to 2.12-fold and 1.15-fold, respectively, at a higher dose of 2 mg BHA day−1 animal−1, compared with the Fe-NTA-treated groups. Pretreatment with BHA prior to Fe-NTA treatment increased GSH and the activities of antioxidant enzymes to a range of 1.5- to 2-fold in kidney. The results indicate that BHA suppresses Fe-NTA-induced nephrotoxicity in male Wistar rats.

scholarly journals Oxidative stress in moss Bryum caespiticium (Bryaceae) under the influence of high temperature and light intensity in a technogenically transformed environment

2021 ◽  
Vol 12 (4) ◽  
pp. 710-717
Author(s):  
O. L. Baik ◽  
N. Y. Kyyak ◽  
O. M. Humeniuk ◽  
V. V. Humeniuk
Keyword(s):  
Oxidative Stress ◽  
Thermal Stability ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
High Temperatures ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Extreme Temperatures ◽  
Thermal Stability Of

Mosses are pioneer plants in post-technogenic areas. Therefore, the question of adaptive reactions of mosses from these habitats represents a scientific interest. The research is devoted to the study of adaptive changes in the metabolism of the dominant moss species Bryum caespiticium Hedw., collected in the devastated territories of the Novoyavorivsk State Mining and Chemical Enterprise (SMCE) “Sirka (Sulfur)” exposed to hyperthermia and insolation, which cause oxidative stress in plants. The influence of these stressors on the activity and thermal stability of antioxidant enzymes, hydrogen peroxide content, anion radical generation and accumulation of prooxidant components in moss shoots was studied. The activity and thermal stability of peroxidase and superoxide dismutase (SOD) were analysed forB. caespiticium moss from different locations of northern exposure at the sulfur mining dump No 1 in summer and autumn. We established the dependence of the activity of antioxidant enzymes of moss on the intensity of light and temperature on the experimental plots of the dump No 1. In summer, the highest activity and thermal stability rates of peroxidase and SOD were observed. Under the conditions of the experiment in shoots of В. caespiticium from the northern peak of the dump under the influence of 2 hours temperature action (+ 42 ºС) the most significant increase in peroxidase activity was found by 1.78 times and SOD by 1.89 times, as well as increase in its thermal stability by 1.35–1.42 times, respectively. The increase in peroxidase and SOD activity, as well as the increase in their thermal stability caused by hyperthermia were negated by pre-processing with a protein biosynthesis inhibitor cyclohexamide, which may indicate the participation of the protein-synthesizing system in this process. The effect of increasing the thermal stability of enzymes can be considered as a mechanism of adaptation of the protein-synthesizing system to the action of high temperatures. Increase in the activity and thermal stability of antioxidant enzymes is caused primarily by changes in the expression of stress protein genes, which control the synthesis of specific adaptogens and protectors. The obtained results indicate that the extreme conditions of the anthropogenically transformed environment contribute to the development of forms with the highest potential abilities. The mechanism of action of high temperatures is associated with the development of oxidative stress, which is manifested in the intensification of lipid peroxidation and the generation of superoxide anion radical. It was found that temperature stress and high insolation caused an increased generation of superoxide anion radical as the main inducers of protective reactions in the samples of B. caespiticium from the experimental transect of the sulfur mining heap. It is known that the synthesis of Н2О2 occurs under stress and is a signal to start a number of molecular, biochemical and physiological processes of cells, including adaptation of plants to extreme temperatures. It is shown that high temperatures initiate the generation of hydrogen peroxide. Increased reactive oxygen species (ROS) formation, including Н2О2, under the action of extreme temperatures, can cause the activation of signaling systems. Therefore, the increase in the content of Н2О2 as a signaling mediator is a component of the antioxidant protection system. It is determined that adaptive restructuring of the metabolism of the moss В. caespiticium is associated with the accumulation of signaling prooxidant components (diene and triene conjugates and dienketones). The increase in primary lipid peroxidation products, detected by us, under the action of hyperthermia may indicate the intensification of free radical oxidation under adverse climatic conditions in the area of the sulfur production dump, which leads to the intensification of lipid peroxidation processes. The accumulation of radical and molecular lipid peroxidation products are signals for the activation of protective systems, activators of gene expression and processes that lead to increased resistance of plants.

scholarly journals Effects of Nonylphenol-Induced Oxidative Stress in Ovary of Cichlid Fish, Etroplus maculatus (Bloch, 1795)

2016 ◽  
Vol 58 ◽  
pp. 11-15 ◽  
Author(s):  
K.P. Asifa ◽  
K.C. Chitra
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Reductase ◽  
Cichlid Fish ◽  
Time Dependent ◽  
Dependent Manner ◽  
Treatment Groups ◽  
Hydrogen Peroxide Generation

The present study was designed to evaluate the effects of nonylphenol in the pro-oxidant/ antioxidant system in ovary of the cichlid fish Etroplus maculatus. Fishes were exposed at two sublethal concentrations (one-fifth and one-tenth of LC50) of nonylphenol for 24, 72 and 96 h maintaining control groups. The oxidative stress indices as the activities of antioxidant enzymes, superoxide dismutase, catalase, glutathione reductase along with the levels of hydrogen peroxide generation and lipid peroxidation were monitored in concentration-and time-dependent manner. Activity of superoxide dismutase significantly (P<0.05) increased at both concentrations in time-dependent manner. Meanwhile the activities of catalase and glutathione reductase significantly (P<0.05) decreased after 72 and 96 h of nonylphenol treatment. The levels of hydrogen peroxide generation and lipid peroxidation increased in all treatment groups when compared to controls. The present results demonstrated that the induction of oxidative stress in ovary of fish by the generation of lipid peroxidation could be due to the exposure of environmental contaminant, nonylphenol. Therefore, the observed oxidative stress in ovary can be indicated as a mechanism of toxicity in the fish exposed to nonylphenol.

The ethanol leaf extract of Andrographis paniculata blunts acute renal failure in cisplatin-induced injury in rats through inhibition of Kim-1 and upregulation of Nrf2 pathway

2018 ◽  
Vol 30 (2) ◽  
pp. 205-217 ◽  
Author(s):  
Bisi O. Adeoye ◽  
Ademola A. Oyagbemi ◽  
Ebunoluwa R. Asenuga ◽  
Temidayo O. Omobowale ◽  
Adeolu A. Adedapo
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Serum Creatinine ◽  
Leaf Extract ◽  
Histopathological Examination ◽  
Hematological Parameters ◽  
Kidney Tissue ◽  
Inflammatory Cells ◽  
Andrographis Paniculata ◽  
Markers Of Oxidative Stress

Abstract Background Cisplatin (CP) is a novel drug of choice in the treatment of cancer but its major limitation is nephrotoxicity, which is dose limiting. Andrographis paniculata (AP) is a common Indian dietary component. It is well known for its medicinal properties. This present study investigated the nephroprotective effect of ethanol leaf extract of Andrographis paniculata (EEAP) on CP-induced nephrotoxicity. Methods CP was used to induce nephrotoxicity in male Wistar rats to study the effect of EEAP on renal damages using hematological parameters, biochemical parameters, histology, and immunohistochemistry studies. Results The effects of EEAP were determined by CP-induced changes in different kidney tissue on antioxidant enzymes, markers of oxidative stress, serum creatinine, and urine parameters. Administration of EEAP (200 mL/kg and 400 mg/kg orally), prior to and following a single dose CP treatment (10 mg/kg i.p), significantly mitigated the CP-induced decrease in antioxidant enzymes, and increase in markers of oxidative stress, serum creatinine, and urinary protein. On histopathological examination of the kidney tissue, there was severe glomerular degeneration and infiltration of inflammatory cells in CP only treated rats, mild glomerular degeneration, and infiltration of inflammatory cells in EEAP pre-treated rats. Furthermore, EEAP activated Nrf2 and mitigated Kim-1 pathways in CP-induced nephrotoxicity. Conclusions The results showed the protective effect of EEAP against CP-induced nephrotoxicity.

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