Production of bacterial cellulose biopolymers in media containing rice and corn hydrolysate as carbon sources

2021 ◽  
Vol 29 (9_suppl) ◽  
pp. S1466-S1474
Author(s):  
Karina C de Souza ◽  
Gabriela R dos Santos ◽  
Felipe CS Trindade ◽  
Andréa Fernanda de S Costa ◽  
Yeda MB de Almeida ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Maximum Yield ◽  
Carbon Sources ◽  
Morphological Structure ◽  
Glucose Production ◽  
Infrared Spectrometry ◽  
Random Arrangement ◽  
Rice Medium ◽  
Uniform Manner ◽  
Corn Flakes

Bacterial cellulose (BC) is a promising option for ecologically viable processes. In this work, BC was produced using starch hydrolysate (rice and corn flakes) as carbon sources. The starch was hydrolyzed by fungus for glucose production. A maximum yield of 2.80 ± 0.43 g/L of BC was obtained with the hydrolyzed rice medium. Moreover, BC produced in the rice and corn media had crystallinity of around 50%. Fourier-transform infrared spectrometry confirmed the functional groups in BC as well as the absence of contaminants from production process. The thermogravimetric analysis revealed that the polymers formed with alternative media had greater thermal stability. Scanning electron microscopy revealed a morphological structure with the random arrangement of nanofibrils in a non-uniform manner distributed in the weave of both polymeric films. The biofilms produced in different media had similar properties to those of films obtained with a conventional medium, revealing that the polymeric characteristics are not modified.

scholarly journals Factors Influence on the yield of Bacterial Cellulose of Kombucha (Khubdat Humza)

2014 ◽  
Vol 11 (3) ◽  
pp. 1420-1428 ◽  
Author(s):  
Baghdad Science Journal
Keyword(s):  
Bacterial Cellulose ◽  
Maximum Yield ◽  
Carbon Sources ◽  
Black Tea ◽  
Acetic Acid Bacteria ◽  
Gelling Agent ◽  
Highest Weight ◽  
Dispersing Agent ◽  
Higher Temperature ◽  
C 50

Kombucha(Khubdat Humza) is composed of yeast and acetic acid bacteria especially, Acetobacter xylinum which forms a cellulose pellicle on tea broth. Kombucha(Khubdat Humza) produces bacterial cellulose pellicles, with unique purity and fine structure. It can be used in many forms, such as an emulsifier, stabilizer, dispersing agent, thickener and gelling agent but these are generally subsidiary to its most important use of holding on to water. Recently, bacterial cellulose is used in many special applications such as a scaffold for tissue engineering of cartilages and blood vessels, also for artificial skin for temporary covering of wounds, as well as its used in the clothing industry. The yield of cellulose produced were investigated in this study, the tea broth was fermented naturally over a period of up to 20 days in the presence of different amounts of black tea and sucrose as nitrogen and carbon sources. 10g/L black tea produced highest weight of bacterial cellulose (55.46g/L) and 100g/L sucrose also exhibited high amount of pellicle (63.58g/L). Temperature was essential factor on growth, where the pellicle was formed at range (20°C - 50°C) and higher temperature over 50°C depressed the bacterial cellulose formation. The bacterial cellulose production increased with the increase of surface area and depth of the broth. Findings from this study suggest that the yield of cellulose depends on many factors that need to be optimized to achieve maximum yield.

Fabrication of Conductive Textile via Chemical Oxidative Polymerization of Aniline on Polyester Cloth

2018 ◽  
Author(s):  
alireza razaghi
Keyword(s):  
Acidic Solution ◽  
Oxidative Polymerization ◽  
Morphological Structure ◽  
Infrared Spectrometry ◽  
Aniline Monomer ◽  
Polyester Textile ◽  
Conductive Polyaniline ◽  
Conductive Textile ◽  
Polymerization Conditions ◽  
Aniline Polymerization

In this research aniline polymerization conditions were optimized in presence of pre-treated polyester textile to achieve as high electrical conductivity as 100 S/Cm. Alkaline activation of the polyester textile was followed by immersion in to aqueous acidic solution of aniline monomer. Then the oxidant solution was used to initiate the polymerization. Finally, the prepared product was washed and dried prior to ant test. Functional groups were studied by Fourie-transformed infrared spectrometry (FTIR) from the surface of the polyaniline coated textile. Also, morphological structure of synthesized conductive polyaniline was studied by scanning electron microscopy (SEM). The synthesized cloth was used in a closed circuit in order to light up alight emitting diode to emphasis the conductivity of the textile and fibres that synthesised by this method.

Fabrication of Conductive Textile via Chemical Oxidative Polymerization of Aniline on Polyester Cloth

2018 ◽  
Author(s):  
alireza razaghi
Keyword(s):  
Acidic Solution ◽  
Oxidative Polymerization ◽  
Morphological Structure ◽  
Infrared Spectrometry ◽  
Aniline Monomer ◽  
Polyester Textile ◽  
Conductive Polyaniline ◽  
Conductive Textile ◽  
Polymerization Conditions ◽  
Aniline Polymerization

In this research aniline polymerization conditions were optimized in presence of pre-treated polyester textile to achieve as high electrical conductivity as 100 S/Cm. Alkaline activation of the polyester textile was followed by immersion in to aqueous acidic solution of aniline monomer. Then the oxidant solution was used to initiate the polymerization. Finally, the prepared product was washed and dried prior to ant test. Functional groups were studied by Fourie-transformed infrared spectrometry (FTIR) from the surface of the polyaniline coated textile. Also, morphological structure of synthesized conductive polyaniline was studied by scanning electron microscopy (SEM). The synthesized cloth was used in a closed circuit in order to light up alight emitting diode to emphasis the conductivity of the textile and fibres that synthesised by this method.

Effect of Solid to Water Ratio, Time and Temperature on Aqueous Extraction of Gallic Acid from Labisia pumila var alata of Malaysia

2020 ◽  
Vol 63 (2) ◽  
pp. 93-99
Author(s):  
Noor Adilah Md Salehan ◽  
Aishath Naila ◽  
Azilah Ajita ◽  
Ahmad Ziad Sulaiman
Keyword(s):  
Optimum Condition ◽  
Gallic Acid ◽  
Maximum Yield ◽  
Morphological Structure ◽  
Aqueous Extraction ◽  
Reference Standard ◽  
Liquid Chromatograph ◽  
Acid Yield ◽  
Sample Matrix ◽  
Water Ratio

  Labisa pumila var alata contains bioactive compounds such as gallic acid that is of use for pharmaceutical and nutraceutical industries. Aqueous extraction is the traditional method that extracts phytochemicals from plant material. The study aimed to find the optimum condition for maximum gallic acid yield from ground L. pumila leaves using aqueous extraction. The results revealed that the maxium gallic acid yield obtained was at 1:10 sample to water ratio for 8 h at 50 °C. The maximum yield of gallic acid obtained was 1.025 mg gallic acid per g dried leaves (mg/g). The identification of gallic acid was done on Liquid Chromatograph Mass Spectrometer Quadrupole Time-of-Flight (LCMS-Q-TOF) by comparison to that of reference standard. The morphological structure of the extract that was obtained at optimum condition showed less denaturation of cell wall which indicates that still some gallic acid could be trapped in the sample matrix and other methods need to be employed to release them.    

scholarly journals Cellulose Nanopaper Cross-Linked Amino Graphene/Polyaniline Sensors to Detect CO2 Gas at Room Temperature

Sensors ◽  
2019 ◽  
Vol 19 (23) ◽  
pp. 5215 ◽  
Author(s):  
Hanan Abdali ◽  
Bentolhoda Heli ◽  
Abdellah Ajji
Keyword(s):  
Bacterial Cellulose ◽  
Room Temperature ◽  
Morphological Structure ◽  
High Sensitivity ◽  
Fast Response ◽  
Aniline Monomer ◽  
Resistance Response ◽  
Covalent Interaction ◽  
Response Characteristics ◽  
Carbon Dioxide Co2

A nanocomposite of cross-linked bacterial cellulose–amino graphene/polyaniline (CLBC-AmG/PANI) was synthesized by covalent interaction of amino-functionalized graphene (AmG) AmG and bacterial cellulose (BC) via one step esterification, and then the aniline monomer was grown on the surface of CLBC-AmG through in situ chemical polymerization. The morphological structure and properties of the samples were characterized by using scanning electron microscopy (SEM), and thermal gravimetric analyzer (TGA). The CLBC-AmG/PANI showed good electrical-resistance response toward carbon dioxide (CO2) at room temperature, compared to the BC/PANI nanopaper composites. The CLBC-AmG/PANI sensor possesses high sensitivity and fast response characteristics over CO2 concentrations ranging from 50 to 2000 ppm. This process presents an extremely suitable candidate for developing novel nanomaterials sensors owing to easy fabrication and efficient sensing performance.

Production of Bacterial Cellulose Using Different Carbon Sources by Two-Stage Cultivation Strategy

2011 ◽  
Vol 201-203 ◽  
pp. 722-725 ◽  
Author(s):  
Jun Hong Lin ◽  
Yi Jen Lin ◽  
Jui Chih Kuo ◽  
Ting Yu Chen ◽  
Wen Pei Sung
Keyword(s):  
Bacterial Cellulose ◽  
Reducing Sugars ◽  
Cell Concentration ◽  
Carbon Sources ◽  
Gluconacetobacter Xylinus ◽  
Glucose Medium ◽  
Two Stage ◽  
Second Stage ◽  
Cultivation Strategy ◽  
Cell Volumes

The production of bacterial cellulose (BC) from Gluconacetobacter xylinus could be improved by the two-stage cultivation strategy. The jar fermentor was applied at first stage to increase cell concentration. At the second stage, the bacteria statically grew within beaker. Three different fraction of cell volumes were cultured in modified YPD-glucose medium or YPD-molasses one. In the modified YPD-molasses medium, the BC yield were highest in this study when the consuming rate of reducing sugars (RS) was 0.181 g-dry BC L-1 d-1 and the BC production rate was 0.183 g-dry BC/g-RS, respectively. The modified YPD-glucose medium could get the maximum pellicle thickness of 5.56±0.64 mm and water content of 99.4%, respectively.

scholarly journals Bacterial Cellulose: Production, Modification and Perspectives in Biomedical Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 1352 ◽  
Author(s):  
Gorgieva ◽  
Trček
Keyword(s):  
Bacterial Cellulose ◽  
Bone Repair ◽  
High Surface ◽  
Carbon Sources ◽  
High Surface Area ◽  
Young Modulus ◽  
High Water ◽  
Ex Situ ◽  
Bacterial Strains ◽  
High Flexibility

Abstract: Bacterial cellulose (BC) is ultrafine, nanofibrillar material with an exclusive combination of properties such as high crystallinity (84%–89%) and polymerization degree, high surface area (high aspect ratio of fibers with diameter 20–100 nm), high flexibility and tensile strength (Young modulus of 15–18 GPa), high water-holding capacity (over 100 times of its own weight), etc. Due to high purity, i.e. absence of lignin and hemicellulose, BC is considered as a non-cytotoxic, non-genotoxic and highly biocompatible material, attracting interest in diverse areas with hallmarks in medicine. The presented review summarizes the microbial aspects of BC production (bacterial strains, carbon sources and media) and versatile in situ and ex situ methods applied in BC modification, especially towards bionic design for applications in regenerative medicine, from wound healing and artificial skin, blood vessels, coverings in nerve surgery, dura mater prosthesis, arterial stent coating, cartilage and bone repair implants, etc. The paper concludes with challenges and perspectives in light of further translation in highly valuable medical products.

Glucose production, recycling, Cori cycle, and gluconeogenesis in humans: relationship to serum cortisol

1997 ◽  
Vol 272 (3) ◽  
pp. E476-E484 ◽  
Author(s):  
J. A. Tayek ◽  
J. Katz
Keyword(s):  
Cancer Patients ◽  
Plasma Cortisol ◽  
Tricarboxylic Acid Cycle ◽  
Carbon Sources ◽  
Glucose Production ◽  
Serum Cortisol ◽  
Normal Subjects ◽  
Tricarboxylic Acid ◽  
Cori Cycle ◽  
Gas Chromatography Mass Spectroscopy

Six normal subjects (NL group) and 13 cancer patients (CAI and CAII groups) were fasted overnight and infused with [U-(13)C]glucose (0.016-0.058 mg x min(-1) x kg(-1)). Plasma glucose and lactate were isolated, and mass isotopomer distributions were determined by gas chromatography-mass spectroscopy. Applying equations modified from those previously described [J. A. Tayek and J. Katz. Am. J. Physiol. 270 (Endocrinol. Metab. 33): E709-E717, 1996], we determined glucose production (GP), recycling of glucose carbons, fraction of recycled molecules in blood glucose (Cori cycle), formation of pyruvate from unlabeled carbons, dilution of pyruvate via the tricarboxylic acid cycle and other reactions, and rate of gluconeogenesis. Glucose production was similar in all groups: 2.4 +/- 0.2 mg x min(-1) x kg (-1). The fraction of recycled carbon and of the Cori cycle were elevated in the CAI group vs. the CAII and NL groups: 15 and 33% vs. 7.8 and 19%, respectively (P = 0.01). Gluconeogenesis was 1.9 +/- 0.1, 1.0 +/- 0.1, and 0.83 +/- 0.11 mg x min(-1) x kg(-1) in the CAI, CAII, and NL groups, respectively. In the NL and CAII groups, 20% of GP is via recycling, 20% from unlabeled carbon sources (muscle glycogen, amino acids), and 60% from hepatic glycogenolysis; in the CAI group, 30% is from recycling, 50% from unlabeled carbon, and 20% from glycogen and other sources. Serum cortisol was elevated in the CAI group vs. the CAII group: 11.2 +/- 1.2 vs. 7.7 +/- 1.2 microg/dl (P < 0.05). There was a strong correlation between plasma cortisol and Cori cycle in the NL (r = 0.963) and CAI and CAII groups (r = 0.771). Serum cortisol was directly, and insulin was inversely, correlated with gluconeogenesis in the NL (r2 = 0.967) and CAI and CAII groups (r2 = 0.727). We conclude that whereas the cancer population is heterogeneous with respect to gluconeogenesis, many cancer patients derive their GP predominantly from gluconeogenesis compared with healthy controls, who derive less than one-half of their GP from gluconeogenesis.

scholarly journals Production and action of an Aspergillus phoenicis enzymatic pool using different carbon sources

2012 ◽  
Vol 15 (3) ◽  
pp. 253-260 ◽  
Author(s):  
Vivian Machado Benassi ◽  
Rosymar Coutinho de Lucas ◽  
Michele Michelin ◽  
João Atílio Jorge ◽  
Héctor Francisco Terenzi ◽  
...  
Keyword(s):  
Reducing Sugars ◽  
Culture Media ◽  
Carbon Sources ◽  
Industrial Wastes ◽  
Cassava Flour ◽  
Aspergillus Phoenicis ◽  
Enzymatic Systems ◽  
Corn Flakes ◽  
Different Origins ◽  
Layer Chromatography

Aspergillus phoenicis is an interesting heat tolerant fungus that can synthesize enzymes with several applications in the food industry due to its great hydrolytic potential. In this work, the fungus produced high enzymatic levels when cultivated on inexpensive culture media consisting of flakes from different origins such as cassava flour, wheat fibre, crushed soybean, agro-industrial wastes, starch, glucose or maltose. Several enzymatic systems were produced from these carbon sources, but amylase was the most evident, followed by pectinase and xylanase. Traces of CMCases, avicelase, lipase, β-xylosidase, β-glucosidase and α-glucosidase activities were also detected. Amylases were produced on rye flakes, starch, oat flakes, corn flakes, cassava flour and wheat fibre. Significant amylolytic levels were produced in the culture medium with glucose or when this sugar was exhausted, suggesting an enzyme in the constitutive form. Cassava flour, rye, oats, barley and corn flakes were also used as substrates in the hydrolytic reactions, aiming to verify the liberation potential of reducing sugars. Corn flakes induced greater liberation of reducing sugars as compared to the others. Thin layer chromatography of the reaction end products showed that the hydrolysis of cassava flour liberated maltooligosaccharides, but cassava flour and corn, rye, oats and barley flakes were hydrolyzed to glucose. These results suggested the presence of glucoamylase and α-amylase as part of the enzymatic pool of A. phoencis.

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