scholarly journals Feline Leukemia Virus Detection by ELISA and PCR in Peripheral Blood from 68 Cats with High, Moderate, or Low Suspicion of having FeLV-Related Disease

1996 ◽  
Vol 8 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Marion L. Jackson ◽  
Deborah M. Haines ◽  
Susan M. Taylor ◽  
Vikram Misra
Keyword(s):  
Relative Risk ◽  
Peripheral Blood ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Positive Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Related Disease ◽  
Significant Difference ◽  
V Antigen

Clinicopathologic criteria were used to group 68 cats according to high, moderate, or low suspicion of having feline leukemia virus (FeL V)-related disease. Peripheral blood samples were tested for FeL V antigen by enzyme-linked immunosorbent assay (ELISA) and for FeL V DNA by polymerase chain reaction (PCR). There was no significant difference between ELISA and PCR results in the 68 cats. In the high-suspicion group, 46% (11/24) of cytopenic cats were test positive (ELISA and PCR) and 87% (13/15) with hemopoietic neoplasms were test-positive. Also within the high suspicion group, test-positive cats were 2.5 times more likely to die within the 1 year follow-up period than were test-negative (ELISA and PCR) cats. Among cats in the moderate-suspicion group, 15% (2/13) were test-positive, and none (0/16) of the cats in the low suspicion group was test positive. The relative risk of a positive test (ELISA and PCR) in the high suspicion group was 3.7 times that for the moderate-suspicion group and 22.8 times that for the low suspicion group. There was no significant difference in the relative risk of a positive test result between the moderate and low suspicion groups. The results indicate that FeL V detection by PCR can be adapted for diagnostic purposes using peripheral blood samples, however, results do not differ significantly from FeL V ELISA results. Also, a proportion of cats with a high suspicion of having FeL V-related cytopenia and hemopoietic tumors are negative for both circulating FeL V antigen and DNA. These cats may not have FeL V-related disease, or FeL V may exist in a disease-producing but nonreplicating form ultimately detectable by PCR in tissues other than peripheral blood.

scholarly journals Prevalence of feline coronavirus, feline leukemia virus, and feline immunodeficiency virus in client-owned cats in Croatia

2021 ◽  
Vol 8 ◽  
pp. 24-38
Author(s):  
Jelena Raukar
Keyword(s):  
Veterinary Medicine ◽  
Feline Immunodeficiency Virus ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Antibody Prevalence ◽  
Blood Samples ◽  
Proviral Dna ◽  
Immunodeficiency Virus ◽  
Feline Coronavirus

This study aimed to determine prevalences for anti-FCoV antibody, FeLV antigen, FeLV proviral DNA, and anti-FIV antibody among client-owned cats from the cities of Zagreb and Varaždin in Croatia. Subjects included 106 client-owned cats tested at the Faculty of Veterinary Medicine, Vienna, Austria. Blood samples were tested with IFA for anti-FCoV antibody and IFA FCoV antibody titeres, with ELISA for FeLV p27 antigen, with PCR for FeLV proviral DNA, and with RIM for anti-FIV antibody. Prevalence of FCoV and FeLV was 41.51% and 6.60%, respectively. A coinfection with FeLV/FCoV and FIV/FCoV prevalence was 7.55% and 5.66%. No cats were coinfected with FIV and FeLV. All three viruses were detected, confirming their presence in Croatia. The seroepidemiological findings demonstrate that both feline retroviruses and feline coronavirus are important feline pathogens in Croatia.

Evaluation of a saliva test kit for feline leukemia virus antigen

1996 ◽  
Vol 32 (5) ◽  
pp. 397-400
Author(s):  
SD Babyak ◽  
MG Groves ◽  
DS Dimski ◽  
J Taboada
Keyword(s):  
Leukemia Virus ◽  
Virus Antigen ◽  
Elisa Test ◽  
Feline Leukemia Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Kit ◽  
Test Kit ◽  
Blood Smears ◽  
Indirect Immunofluorescent ◽  
Saliva Test

An enzyme-linked immunosorbent assay (ELISA) test kit for the detection of feline leukemia virus (FeLV) antigen in saliva was evaluated in 150 cats. Saliva and blood samples from all cats were tested for FeLV using the saliva ELISA kit and a plasma ELISA kit, respectively. These results were compared with indirect immunofluorescent antibody (IFA) testing of blood smears also obtained from each cat. The proportion of cats that tested positive were 10%, 7%, and 8% for each test, respectively. Using the IFA test as the gold standard, the saliva FeLV test had a sensitivity of 91.7% and specificity of 97.1%, while the plasma ELISA test had a sensitivity of 91.7% and specificity of 100%.

scholarly journals Pathophysiological role of Atg5 in human ulcerative colitis

2020 ◽  
Vol 18 (4) ◽  
pp. 421-429
Author(s):  
Razieh Ardali ◽  
Nasrin Kazemipour ◽  
Saeed Nazifi ◽  
Kamran Bagheri Lankarani ◽  
Iman Razeghian Jahromi ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Blood Samples ◽  
Significant Difference ◽  
Colon Biopsy ◽  
Pathophysiological Role ◽  
Healthy Control ◽  
Inflammatory Bowel ◽  
Insight Into

Background/Aims: Ulcerative colitis (UC), along with Crohn’s disease, is one of the main types of inflammatory bowel disease (IBD). On the other hand, deregulated autophagy is involved in many chronic diseases, including IBD. In this study, we aimed to investigate the role of Atg5 and microRNA-181a (miR-181a) in the pathophysiology of UC. Methods: Colon biopsy, stool, and blood samples of 6 men and 9 women were confirmed for UC. Also, 13 men and 17 women were selected as healthy control (HC). Enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry were used to measure the Atg-5 content of the colon biopsies. Besides, the serum and stool levels of Atg5 were measured using ELISA. Moreover, the total RNA of blood cells was extracted and evaluated for the expression of miR-181a.Results: We found 1.2 ng/mL versus 0.46 ng/mL, 0.34 ng/mL versus 0.24 ng/mL, and 0.082 ng/mL versus 0.062 ng/mL of Atg5 in stool, intestinal tissue, and serum of UC and HCs, respectively. There was no significant difference in the expression of miR-181a in the blood samples of UC and HCs. Immunohistochemistry showed high positivity without any significant difference between the 2 groups in the quantitative analysis.Conclusions: The significant difference observed between the stool Atg5 content of the HCs and UC patients may provide new insight into using this protein as a diagnostic biomarker, however, considering the small size of our studied population further studies are needed.

scholarly journals Lack of Bcl-2 expression in feline follicular lymphomas

2019 ◽  
Vol 31 (6) ◽  
pp. 809-817
Author(s):  
Manfred Henrich ◽  
Anna Bauknecht ◽  
Werner Hecht ◽  
Manfred Reinacher
Keyword(s):  
Follicular Lymphoma ◽  
Single Cells ◽  
Leukemia Virus ◽  
Receptor Gene ◽  
Chromosomal Translocation ◽  
Feline Leukemia Virus ◽  
Immunoglobulin Gene ◽  
Follicular Hyperplasia ◽  
Significant Difference ◽  
Follicular Lymphomas

Bcl-2, an anti-apoptotic protein, is commonly overexpressed in follicular lymphomas in humans. This is usually the result of a chromosomal translocation that transposes the Bcl-2 gene into the immunoglobulin gene locus. The immunohistochemical assessment of this overexpression can be used as a tool for the differentiation of follicular lymphoma and follicular hyperplasia. In cats, little information about the expression of Bcl-2 in follicular lymphoma exists. We investigated 18 follicular lymphomas histologically and immunohistochemically for the expression of Bcl-2, CD3, CD45R, and feline leukemia virus. Clonality was assessed by PCR for antigen receptor gene rearrangements. Although the histology resembled that of their human counterparts, diffuse expression of Bcl-2 within the follicles of the feline lymphomas, as seen in human cases, was not present. Only single cells within the follicles, comparable to the reactive controls, were positive for Bcl-2 expression. The mean survival time of 4.6 y confirmed the indolent character of the tumor. None of the clinical parameters assessed were statistically significant predictors of survival. Furthermore, a statistically significant difference in survival of animals with or without anti-neoplastic therapy was also not demonstrable.

scholarly journals Feline Immunodeficiency Virus Infection in a Population of Pet Cats from Southeastern Florida

1989 ◽  
Vol 1 (4) ◽  
pp. 339-342 ◽  
Author(s):  
Harvey Fisch ◽  
Norman H. Altman
Keyword(s):  
Virus Infection ◽  
Feline Immunodeficiency Virus ◽  
Leukemia Virus ◽  
Feline Leukemia Virus ◽  
Blood Samples ◽  
Specific Antibodies ◽  
Feline Immunodeficiency Virus Infection ◽  
Immunodeficiency Virus

Blood samples from 95 randomly selected pet cats that were brought to veterinarians in southeastern Florida were tested for antibodies to feline immunodeficiency virus (FIV). Virus-specific antibodies (indicative of virus infection) were found in 8 of the 95 (8.4%) cats tested. All of the virus-infected cats were males (statistically significant, P ≤ 0.016) and were at least 1 year of age. The 3 most severely ill cats infected with FIV were also infected with feline leukemia virus.

scholarly journals Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Hui Du ◽  
Yonghong Wang ◽  
Yumin Shi ◽  
Jian Yu ◽  
Wen Sun ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Mrna Expression ◽  
Inflammatory Mediators ◽  
Peripheral Blood ◽  
Transforming Growth Factor ◽  
Pediatric Asthma ◽  
Enzyme Linked Immunosorbent Assay ◽  
Significant Difference ◽  
Before And After

Objective. To observe the effects of empirical prescriptions of traditional Chinese medicine (TCM) on inflammatory mediators in pediatric asthma and to explore the underlying molecular mechanism in the treatment of asthma.Methods. A total of 182 children with asthma were randomly placed into either the TCM group (n=97) or the salbutamol and montelukast (SM) group (n=85). Patients in the TCM group were treated with a series of empirical prescriptions of TCM, while those in the SM group received salbutamol and montelukast. Both groups received their respective treatment for 12 weeks. There were 35 patients in TCM group and 34 patients in SM group providing venous blood. Real-time PCR was used to determine the mRNA expression levels of interleukin- (IL-) 10, IL-17, matrix metalloproteinase-9 (MMP-9), and transforming growth factorβ1 (TGF-β1) in peripheral blood mononuclear cells before and after treatment. Enzyme-linked immunosorbent assay was used to measure the levels of IL-10, IL-17, MMP-9, and TGF-β1 in peripheral blood before and after treatment.Results. The mRNA expression of TGF-β1 in the SM group was downregulated (P=0.00) after treatment. No significant differences were found between the TCM group and the SM group after treatment (P>0.05). In the TCM group, the levels of IL-10, IL-17, and MMP-9 significantly decreased after treatment (P=0.01, 0.04, and 0.03, resp.). In the SM group, IL-17, MMP-9, and TGF-β1 levels significantly decreased after treatment (P=0.00, 0.03, and 0.00, resp.). There was no significant difference between the two groups regarding the levels of IL-10, IL-17, TGF-β1, and MMP-9 (P>0.05). The difference of the level of IL-17 was negatively correlated with the change of C-ACT score in TCM group and SM group.Conclusion. TCM has a regulatory effect on the balance of some inflammatory mediators in pediatric asthma.

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