Cognitive Impairment and Neurogenotoxic Effects in Rats Exposed to Low-Intensity Microwave Radiation

2015 ◽  
Vol 34 (3) ◽  
pp. 284-290 ◽  
Author(s):  
Pravin Suryakantrao Deshmukh ◽  
Namita Nasare ◽  
Kanu Megha ◽  
Basu Dev Banerjee ◽  
Rafat Sultana Ahmed ◽  
...  

The health hazard of microwave radiation (MWR) has become a recent subject of interest as a result of the enormous increase in mobile phone usage. The present study aimed to investigate the effects of chronic low-intensity microwave exposure on cognitive function, heat shock protein 70 (HSP70), and DNA damage in rat brain. Experiments were performed on male Fischer rats exposed to MWR for 180 days at 3 different frequencies, namely, 900, 1800 MHz, and 2450 MHz. Animals were divided into 4 groups: group I: sham exposed; group II: exposed to MWR at 900 MHz, specific absorption rate (SAR) 5.953 × 10−4 W/kg; group III: exposed to 1800 MHz, SAR 5.835×10−4 W/kg; and group IV: exposed to 2450 MHz, SAR 6.672 × 10−4 W/kg. All the rats were tested for cognitive function at the end of the exposure period and were subsequently sacrificed to collect brain. Level of HSP70 was estimated by enzyme-linked immunotarget assay and DNA damage was assessed using alkaline comet assay in all the groups. The results showed declined cognitive function, elevated HSP70 level, and DNA damage in the brain of microwave-exposed animals. The results indicated that, chronic low-intensity microwave exposure in the frequency range of 900 to 2450 MHz may cause hazardous effects on the brain.

Author(s):  
Феликс Андреевич Пятакович ◽  
Ольга Владимировна Мевша ◽  
Татьяна Игоревна Якунченко ◽  
Кристина Феликсовна Макконен

Еще в 60-70 годы XX-го столетия в экспериментальных исследованиях новосибирских ученых, выполненных под руководством академика В.П.Казначеева, была установлена роль сверхслабых физических полей в развитии цитопатологического действия биологических объектов вирусной природы, агентов химической и физической природы. В последние годы в экспериментальной и клинической медицине наряду с использованием низкоинтенсивных крайне высокочастотных миллиметровых излучений (менее 10 мВт/см) успешно используют СВЧ генераторы сверхнизкой интенсивности сантиметрового диапазона длин волн на уровне мощности, не превышающих 10 мкВт/см. Подобное СВЧ излучение сверхмалой мощности, осуществляемое на частоте колебаний кластеров воды первого порядка, способствует нормализации процессов метаболизма. Настоящее исследование выполнено в соответствии с планами проблемной комиссии по «хронобиологии и хрономедицине» РАН. На основе междисциплинарного сотрудничества была сформирована виртуальная команда исследователей работающих в разных университетах страны и включавшая математиков, системных программистов, инженеров и врачей-исследователей. При этом для реализации целей и задач исследования использовалась, так называемая, линейная модель движения потока информации из научной лаборатории в клиническую практику (from bench to bedside). В связи с чем, нами был реализован проект, направленный на разработку технической системы модульного типа, предназначенной для генерации СВЧ излучения с заданными свойствами. В микроконтроллере СВЧ генератора был запрограммирован цифровой аналог сигналов отражающих нейродинамические процессы мозга. В настоящей статье показана электрофизиологическая эффективность сверхнизко интенсивных излучений сантиметрового диапазона длин волн модулированных низкочастотными сигналами подобных ритму ЭЭГ и с циклической изменяемостью скважности. В клинических исследованиях у больных сахарным диабетом II с сопутствующей гипертонической болезнью на основе теста Спилбергера показана трансформация патологической нейродинамической активности мозга с последующей модификацией функционального состояния пациента. Рассмотрены результаты не эффективного воздействия, эффективного воздействия и успешного воздействия с изменениями динамики уровня пластичности нейродинамической активности мозга Back in the 60s-70s of the 20th century, in the experimental studies of Novosibirsk scientists, carried out under the leadership of Academician V.P. Kaznacheev, the role of super weak physical fields in the development of the cytopathological action of biological objects of viral nature, agents of chemical and physical nature was established. In recent years, in experimental and clinical medicine, along with the use of low-intensity extremely high-frequency millimeter radiation (less than 10 mW / cm), microwave generators of super low intensity of the centimeter wavelength range have been successfully used at a power level not exceeding 10 μW/cm. Such super low-power microwave radiation, is realized at the frequency of vibrations of first-order water clusters, contributes to the normalization of metabolic processes. The present study was work out in accordance with the plans of the problem commission on "chronobiology and chronomedicine" of the Russian Academy of Sciences. On the basis of interdisciplinary collaboration, a virtual team of researchers working in different universities in the country was formed and included mathematicians, systems programmers, engineers and medical researchers. At the same time, to implement the goals and objectives of the study, the so-called linear model of the flow of information from the scientific laboratory to clinical practice (from bench to bedside) was used. In this connection, we have implemented a project aimed at developing a modular-type technical system designed to generate microwave radiation with desired properties. In the microcontroller of the microwave generator, a digital analogue of signals reflecting the neurodynamic processes of the brain was programmed. This article shows the electrophysiological efficiency of super-low-intensity radiation in the centimeter range of wavelengths modulated by low-frequency signals similar to the EEG rhythm. In experimental studies in patients with diabetes mellitus II with concomitant hypertension, the transformation of the pathological neurodynamic activity of the brain with the subsequent modification of the patient's functional state has been shown. The results of ineffective influence, effective influence and successful influence with changes in the dynamics of the level of plasticity of neurodynamic activity of the brain are considered


2015 ◽  
Vol 51 ◽  
pp. 158-165 ◽  
Author(s):  
Kanu Megha ◽  
Pravin Suryakantrao Deshmukh ◽  
Basu Dev Banerjee ◽  
Ashok Kumar Tripathi ◽  
Rafat Ahmed ◽  
...  

2001 ◽  
Vol 1 (6) ◽  
pp. 483-495 ◽  
Author(s):  
Bentham Science Publisher Philip K. Liu

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Isela Álvarez-González ◽  
Scarlett Camacho-Cantera ◽  
Patricia Gómez-González ◽  
Michael J. Rendón Barrón ◽  
José A. Morales-González ◽  
...  

AbstractWe evaluated the duloxetine DNA damaging capacity utilizing the comet assay applied to mouse brain and liver cells, as well as its DNA, lipid, protein, and nitric oxide oxidative potential in the same cells. A kinetic time/dose strategy showed the effect of 2, 20, and 200 mg/kg of the drug administered intraperitoneally once in comparison with a control and a methyl methanesulfonate group. Each parameter was evaluated at 3, 9, 15, and 21 h postadministration in five mice per group, except for the DNA oxidation that was examined only at 9 h postadministration. Results showed a significant DNA damage mainly at 9 h postexposure in both organs. In the brain, with 20 and 200 mg/kg we found 50 and 80% increase over the control group (p ≤ 0.05), in the liver, the increase of 2, 20, and 200 mg/kg of duloxetine was 50, 80, and 135% in comparison with the control level (p ≤ 0.05). DNA, lipid, protein and nitric oxide oxidation increase was also observed in both organs. Our data established the DNA damaging capacity of duloxetine even with a dose from the therapeutic range (2 mg/kg), and suggest that this effect can be related with its oxidative potential.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
G. Horvath ◽  
G. Kis ◽  
G. Kekesi ◽  
A. Büki ◽  
L. G. Adlan ◽  
...  

AbstractThe low efficacy of antipsychotic drugs (e.g., clozapine) for negative symptoms and cognitive impairment has led to the introduction of adjuvant therapies. Because previous data suggest the procognitive potential of the antidiabetic drug metformin, this study aimed to assess the effects of chronic clozapine and metformin oral administration (alone and in combination) on locomotor and exploratory activities and cognitive function in a reward-based test in control and a schizophrenia-like animal model (Wisket rats). As impaired dopamine D1 receptor (D1R) function might play a role in the cognitive dysfunctions observed in patients with schizophrenia, the second goal of this study was to determine the brain-region-specific D1R-mediated signaling, ligand binding, and mRNA expression. None of the treatments affected the behavior of the control animals significantly; however, the combination treatment enhanced D1R binding and activation in the cerebral cortex. The Wisket rats exhibited impaired motivation, attention, and cognitive function, as well as a lower level of cortical D1R binding, signaling, and gene expression. Clozapine caused further deterioration of the behavioral parameters, without a significant effect on the D1R system. Metformin blunted the clozapine-induced impairments, and, similarly to that observed in the control animals, increased the functional activity of D1R. This study highlights the beneficial effects of metformin (at the behavioral and cellular levels) in blunting clozapine-induced adverse effects.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ruiqing Zhu ◽  
Hui Wang ◽  
Xinping Xu ◽  
Li Zhao ◽  
Jing Zhang ◽  
...  

AbstractPrevious studies have shown that single-frequency microwave radiation can lead to cognitive decline in rats. However, few studies have focused on the combined effects of irradiation with different frequencies of microwaves. Our research aimed to investigate the effects of 1.5 GHz and 4.3 GHz microwave radiation, singly and in combination, on cognitive function and hippocampal tissue structure in rats. A total of 140 male Wistar rats were randomly divided into 4 groups: the S group (sham radiation group), L10 group (10 mW/cm2 1.5 GHz group), C10 group (10 mW/cm2 4.3 GHz band group) and LC10 group (10 mW/cm2 1.5 and 4.3 GHz multi-frequency radiation group). For 1–28 days after microwave radiation, we analyzed the average escape latency for the Morris water maze task, electroencephalograms, change in hippocampal tissue structure and ultrastructure, content of the Nissl body in the hippocampus, and activities of lactate dehydrogenase and succinate dehydrogenase. Compared to the S group, all exposure groups showed varying degrees of learning and memory decline and hippocampal structural damage. The results showed that 1.5 GHz and 4.3 GHz microwave radiation was able to induce cognitive impairment and hippocampal tissue damage in rats and combined radiation with both frequencies caused more serious injuries, but none of these damaging effects varied with microwave frequency.


2004 ◽  
Vol 23 (8) ◽  
pp. 413-419 ◽  
Author(s):  
Cetin Kaymak ◽  
Ela Kadioglu ◽  
Hulya Basar ◽  
Semra Sardas

In this study, genotoxic effects of repeated sevoflurane anaesthesia were investigated in rabbits with or without antioxidant supplementation. Twenty-one New Zealand male rabbits were included in the study and randomized into three groups as: placebo treated (Group I), vitamin E supplemented (Group II) and selenium supplemented (Group III). Vitamin E and selenium were given intraperitoneally for 15 days before anaesthesia treatment. Anaesthesia was administered using 3% sevoflurane in 4 L/min oxygen for a 3-hour period and continued for 3 days. Blood samples were collected before anaesthesia (Sample 1), after the first, second and third days of sevoflurane administration (Sample 2, Sample 3 and Sample 4 respectively) and the last samples were taken 5 days after the last sevoflurane administration (Sample 5). Genotoxic damage was examined using the comet assay. The degree of damage is assessed by grading the cells into three categories of no migration (NM), low migration (LM) and high migration (HM) depending on the fraction of DNA pulled out into the tail under the influence of the electric field. The number of comets in each sample was calculated (1 × number of comets in category NM + 2 × number of comets in category LM + 3 ×number of comets in category HM) and expressed as the total comet score (TCS), which summarizes the damage frequencies. In Group I, a significant increase in the mean TCSs was observed for Samples 3 and 4 as compared with Sample 1. However, there were no significant differences between Samples 1, 2 and 5. The mean TCS of Sample 4 was significantly higher than Sample 1, 2 and 3 in Group II. Group III demonstrated no significant mean TCSs for any experimental conditions. Statistical differences were also observed between the groups with significant P values. This experimental study points out the presence of DNA damage with repeated sevoflurane anaesthesia and the genoprotective role of antioxidant supplementation on DNA damage in mononuclear leukocytes of rabbits by highly sensitive comet assay.


2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Jintanaporn Wattanathorn ◽  
Jinatta Jittiwat ◽  
Terdthai Tongun ◽  
Supaporn Muchimapura ◽  
Kornkanok Ingkaninan

Cerebral ischemia is known to produce brain damage and related behavioral deficits including memory. Recently, accumulating lines of evidence showed that dietary enrichment with nutritional antioxidants could reduce brain damage and improve cognitive function. In this study, possible protective effect ofZingiber officinale, a medicinal plant reputed for neuroprotective effect against oxidative stress-related brain damage, on brain damage and memory deficit induced by focal cerebral ischemia was elucidated. Male adult Wistar rats were administrated an alcoholic extract of ginger rhizome orally 14 days before and 21 days after the permanent occlusion of right middle cerebral artery (MCAO). Cognitive function assessment was performed at 7, 14, and 21 days after MCAO using the Morris water maze test. The brain infarct volume and density of neurons in hippocampus were also determined. Furthermore, the level of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in cerebral cortex, striatum, and hippocampus was also quantified at the end of experiment. The results showed that cognitive function and neurons density in hippocampus of rats receiving ginger rhizome extract were improved while the brain infarct volume was decreased. The cognitive enhancing effect and neuroprotective effect occurred partly via the antioxidant activity of the extract. In conclusion, our study demonstrated the beneficial effect of ginger rhizome to protect against focal cerebral ischemia.


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