Change in haematological and selected biochemical parameters measured in feline blood donors and feline whole blood donated units

Objectives The quality of whole blood (WB) units is influenced by many factors, starting with selection of donors and the method of blood collection. The aim of this study was to investigate the changes that occur in haematological and selected biochemical parameters in blood transferred from a feline blood donor to feline WB unit. Methods Data from 27 feline blood donations were used in this study. Cats were anaesthetised with a combination of tiletamine and zolazepam. Blood (10 ml/kg body weight to a maximum of 60 ml/cat) was collected in citrate–phosphate–dextrose–adenine (CPDA) anticoagulant. Lactated Ringer’s solution (10 ml/kg) was administered intravenously starting halfway through the donation. Haematological and selected biochemical parameters (complete blood count, free haemoglobin, % haemolysis, glucose, sodium, potassium, pH) were measured in the blood donor before donation and in the corresponding donated WB unit soon after collection. Results Significant decreases occurred between blood donor and WB unit in red blood cells (mean difference −1.06 × 1012/l; P <0.0001), haemoglobin (mean difference −1.6 g/dl; P <0.0001), haematocrit (mean difference −4.6%; P <0.0001), red cell distribution width (mean difference −0.9%; P = 0.0003), white blood cells (mean difference −2.17 × 109/l; P <0.0001), pH (mean difference −0.5; P <0.0001) and potassium (mean difference −1.4 mmol/l; P <0.0001). Significant increases occurred between blood donor and WB unit in platelets (mean difference +87.00 ×109/l; P = 0.0039), glucose (mean difference +25.42 mmol/l; P <0.0001) and sodium (mean difference +20 mmol/l; P <0.0001). Conclusions and relevance When using a blood collection protocol with intravenous fluid administration midway through the donation and a CPDA:blood ratio of 1:7, there were significant changes in both the haematological and biochemical characteristics between the blood donors and WB units. The majority of these changes may be the result of the anticoagulants used for storage. Understanding these changes may assist selection of blood donors and help prediction of the characteristics of the donated WB unit.

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Haematological and morphological evaluation of feline whole blood units collected for transfusion purposes

Journal of Feline Medicine and Surgery ◽

10.1177/1098612x18798841 ◽

2018 ◽

Vol 21 (8) ◽

pp. 732-740 ◽

Cited By ~ 2

Author(s):

Eva Spada ◽

Roberta Perego ◽

Luciana Baggiani ◽

Daniela Proverbio

Keyword(s):

Whole Blood ◽

Blood Cells ◽

Morphological Changes ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

In Vivo Studies ◽

Mean Cell Volume ◽

Distribution Width

ObjectivesDespite the increasing availability of feline blood collected and stored for transfusion purposes, few studies have been performed on feline blood units. The aim of this prospective in vitro study was to evaluate haematological and morphological changes in feline blood cells in whole blood units between collection and end of storage.MethodsHaematological examination (red blood cells [RBCs], haemoglobin, haematocrit, red cell distribution width, mean cell volume, mean cell haemoglobin concentration, mean cell haemoglobin, white blood cells [WBCs] and platelet [PLT] count) was performed on 40 non-leukoreduced feline whole blood units at the time of collection (day[D]0) and after storage (D35). The blood was collected into citrate–phosphate–dextrose–adenine anticoagulant-preservative solution using an open system in a veterinary blood bank and stored for 35 days at 4 ± 2°C. Twenty of these feline whole blood units were also analysed for blood cell morphology (normal RBCs, macrocytes, echinocytes, spherocytes, schistocytes, lysed RBCs, RBCs with Heinz bodies and recognisable WBC and PLT count). Differences between the two examination times were statistically analysed.ResultsThere was a statistically significant decrease in WBC and PLT counts after storage at D35 ( P <0.0001 for both). The most significant cellular morphological changes after storage were an increase in echinocyte count ( P = 0.0001), and lysed RBCs ( P <0.0001), and a decrease in normal RBCs ( P <0.0001). Recognisable WBCs – mainly lymphocytes – were present at the end of storage.Conclusions and relevanceThis study showed that significant morphological changes occur in RBCs in feline blood units during storage for 35 days. In vivo studies are required to establish if these changes could affect the ability of stored RBCs to circulate and provide adequate oxygen delivery after transfusion.

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Physiological Parameters to Identify Suitable Blood Donor Cows for Preparation of Platelet Rich Plasma

Animals ◽

10.3390/ani11082296 ◽

2021 ◽

Vol 11 (8) ◽

pp. 2296

Author(s):

Anna Lange-Consiglio ◽

Rosangela Garlappi ◽

Chiara Spelta ◽

Antonella Idda ◽

Stefano Comazzi ◽

...

Keyword(s):

Blood Donor ◽

Blood Donors ◽

Platelet Rich Plasma ◽

Bovine Mastitis ◽

Field Conditions ◽

Blood Collection ◽

Experimental Conditions ◽

Acute Mastitis ◽

Donor Cows ◽

The Ideal

Platelet rich plasma (PRP) has been shown to be beneficial in the treatment of bovine mastitis, with an action comparable to that of antibiotics. Autologous treatment is feasible in experimental conditions but is difficult to apply in field conditions, particularly in acute mastitis. The ideal scenario would be to have heterologous PRP stored on every farm so that it is readily available when needed. In this paper, we analysed data collected during bovine mastitis treatment with heterologous PRP produced by casual donor cows on several farms. We tried to identify parameters which might be useful to identify the most suitable cows to be used as blood donors, to obtain the highest yield of PRP. Variables considered for each animal were the age, the parity, the date of the last parturition, the season of blood collection, the site of blood collection (jugular or mammary vein) and the reproductive status e.g., pregnant or not pregnant. There were statistically significant differences for all the variables considered from the 135 blood cows, except for the blood collection season. The highest yield of PRP was associated with nonpregnancy blood collection within three months of parturition, parity 3 or 4, and blood collection from the mammary vein.

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Use of whole blood directly for single-cell gel electrophoresis (comet) assay in vivo and white blood cells for in vitro assay

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/j.mrgentox.2004.07.013 ◽

2004 ◽

Vol 564 (1) ◽

pp. 75-82 ◽

Cited By ~ 46

Author(s):

Cheng-Hung Chuang ◽

Miao-Lin Hu

Keyword(s):

Comet Assay ◽

Whole Blood ◽

Blood Cells ◽

Gel Electrophoresis ◽

White Blood Cells ◽

In Vitro Assay ◽

Vitro Assay ◽

Single Cell Gel Electrophoresis

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EFFECTS OF BLOOD CELLS ON PLATELET AGGREGATION BY IMPEDANCE METHOD

10.1055/s-0038-1644870 ◽

1987 ◽

Author(s):

L Mannucci ◽

R Redaelli ◽

E Tremoll

Keyword(s):

Platelet Aggregation ◽

Whole Blood ◽

Blood Cells ◽

White Blood Cells ◽

Normal Subjects ◽

Impedance Method ◽

Induced Aggregation ◽

Vitro Data ◽

In Vitro Data

To evaluate the effects of blood cells on the response of platelets to aggregating agents using whole blood impedance aggregometer, studies were carried out on whole blood (WB) of normal subjects and of patients with: polycythemia vera (PV), iatrogenic anemia (IA), primary thrombocytosis (PT), idiopathic thrombotic purpura (ITP), myeloid chronic leukemia (MCL), iatrogenic leukopenia (IL). The in vitro effects of red blood cells (RBC) and of white blood cells (WBC) on platelet rich plasma (PRP) aggregation were also evaluated. WB, PRP, WBC and RBC were prepared by conventional methods. Aggregation was performed using the impedance aggregometer (mod. 540, Chrono Log Corp). In normal subjects the concentration of collagen giving 50 % aggregation (AC50 ) found in PRP did not differ from that of WB, indicating that hematocrit values within the normal range did not appreciably affect platelet aggregation. The results obtained in WB of patients are summarized in the table: In vitro data showed that aggregation in prp in wb of normal subjects was related to the number of platelets present in the sample. RBC added to PRP significant reduced aggregation only when the RBC number was greater than 4.101 cells. No effect of WBC on collagen induced aggregation of PRP was observed, whereas significant inhibition was detected after ADP. It is concluded that the aggregation evaluated in WB with impedance method is dependent on the platelet number. Also, in vitro data and studies in WB of patients indicate that aggregation is significantly affected by the presence of cells other than platelets only in conditions of changes of the ratio between platelets and leukocytes and/or red cells.

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Detection of Anti-Human T-Lymphotropic Virus Type I Antibody in Whole Blood by a Novel Counting Immunoassay

Clinical Chemistry ◽

10.1373/49.2.275 ◽

2003 ◽

Vol 49 (2) ◽

pp. 275-280 ◽

Cited By ~ 5

Author(s):

Kazunari Yamaguchi ◽

Yuji Yonemura ◽

Hiroaki Okabe ◽

Yoichi Takahama ◽

Shinya Nagai ◽

...

Keyword(s):

Virus Type ◽

Whole Blood ◽

Blood Cells ◽

Measuring Method ◽

Sample Volume ◽

Latex Agglutination ◽

Blood Collection ◽

Type I ◽

Latex Particles ◽

T Lymphotropic Virus

Abstract Background: Assays to screen for and confirm the presence of the antibody for human T-lymphotropic virus type I (HTLV-I) are currently performed with serum or plasma. We developed and evaluated a new counting immunoassay (CIA) for the detection of HTLV-I antibody in whole blood, using recombinant and synthetic peptide antigens. Methods: We assessed the CIA for detection of HTLV-I antibody in whole blood and plasma. The CIA is an immunity-measuring method that combines latex agglutination with particle-counting technology. The numbers of agglutinated latex particles, single latex particles, and blood cells in a sample are measured based on differences in particle size between latex particles and blood cells. Results: The CIA and ELISA methods were in agreement for all 24 plasma samples tested, including those from 6 patients with HTLV-I-associated diseases, 6 HTLV-I carriers, and 12 HTLV-I antibody-negative individuals. The concordance between the ELISA (plasma) and the CIA (whole blood) for samples from 24 patients was 100%. The concordance between a particle agglutination method (plasma) and the CIA (plasma or whole blood) for 1065 patients was 99.5%. The concordance between results obtained for 1065 pairs of plasma and whole blood samples with the CIA method was 100%. HTLV-I antibody in whole blood was stable for 3 days after blood collection. With this CIA method, results were available within 15 min. Conclusions: The CIA method can be used in screening for HTLV-I. The use of whole blood rather than serum or plasma reduces the sample volume and number of blood collections required, as well as assay time.

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Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

European Journal of Agriculture and Food Sciences ◽

10.24018/ejfood.2020.2.5.133 ◽

2020 ◽

Vol 2 (5) ◽

Author(s):

Y. Garba ◽

E. A. Adeola

Keyword(s):

Blood Cells ◽

Biochemical Parameters ◽

White Blood Cells ◽

Haemoglobin Concentration ◽

Serum Biochemical Parameters ◽

Corpuscular Haemoglobin ◽

Randomized Design ◽

Serum Biochemical ◽

Mean Corpuscular Haemoglobin ◽

Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

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Effect of aspartame on biochemical and oxidative stress parameters in rat blood

Archives of Biological Sciences ◽

10.2298/abs141009016p ◽

2015 ◽

Vol 67 (2) ◽

pp. 535-545 ◽

Cited By ~ 9

Author(s):

Marko Prokic ◽

Milica Paunovic ◽

Milos Matic ◽

Natasa Djordjevic ◽

Branka Ognjanovic ◽

...

Keyword(s):

Oxidative Stress ◽

Red Blood Cells ◽

Blood Cells ◽

Biochemical Parameters ◽

White Blood Cells ◽

Lipid Peroxides ◽

Hdl Cholesterol ◽

Albino Rats ◽

Total Body Mass ◽

Antioxidative Status

Aspartame (ASP) is one of the most widely used nonnutritive sweeteners. This study investigates the chronic effects of ASP on hematological and biochemical parameters, and its effects on the oxidative/antioxidative status in the red blood cells of Wistar albino rats. Rats were provided with ASP (40 mg/kg/daily for six weeks) in drinking water. Increased food and fluid intake was observed in the ASP-treated rats. Total body mass was significantly decreased in the ASP-treated rats. Treatment with ASP caused an increase in the concentrations of glucose, cholesterol, LDL-cholesterol, and in the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH), as well as a decrease in the levels of HDL-cholesterol in the serum. A significant decline in the number of white blood cells (WBC) was observed after ASP uptake. Based on the results we conclude that ASP induces oxidative stress, observed as an alteration of the glutathione redox status, which leads to increased concentrations of nitric oxide (NO) and lipid peroxides (LPO) in the red blood cells. Changes in biochemical parameters, lipid metabolism, as well as changes in the levels of oxidative stress markers and the appearance of signs of liver damage indicate that chronic use of ASP can lead to the development of hyperglycemia, hypercholesterolemia and associated diseases.

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EFFECT OF ZINC ELEMENT AND DEXAMETHASONE ON SOME HEMATOLOGICAL BIOCHEMICAL TESTS IN RABBITS MALE

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v47i6.484 ◽

2016 ◽

Vol 47 (6) ◽

Author(s):

D. S. Dheyab

Keyword(s):

Blood Cells ◽

White Blood Cells ◽

The Body ◽

Control Group ◽

Blood Collection ◽

Biochemical Tests ◽

Histological Changes ◽

Spleen Tissue ◽

Cholesterol Levels ◽

Short Time

This study was conducted to investigate the effect of zinc in dose 15mg/kg.bw daily taken by the mouth and dexamethasone 4mgIkg.Bw by injection for 30days on some hematological biochemical tests and some histological changes of liver spleen in male rabbits. Thirty rabbits were used that divided into 3 randomized groups (each group contain 10 male rabbits ). Control group was taken normal food and water, Zinc group that gave zinc at dose of 15mg/kg.BW/daily/oral on 1, 2, 3, 4 weeks. Dexamethasone with zinc group : Employ dexamethasone 4mg/Kg.Bw . I.M dialy for 1 and 2 weeks for experiment and at 3, 4th weeks they gave zn 15mg/lKg.Bw day/orally. Blood samples were taken from the heart directly in 2 and 4weeks to examine packed cell volume (pcv), white blood cells (WBCs), Red blood cells (RBCs) with differential Leuckcyte count.separation blood collection to plasma and examine glucose mg/dl , cholesterol mg/dl. In histological tests, rabbits were killed and separate their organs tissue from the body to examine liver and spleen. The results revealed a decrease in level of RBCs, pcv after treatment with zinc 15, mg/Kg.Bw orally (zinc group) and increase in WBCs with differential leuckocyte count specially neutrophil cell, while biochemical tests show increase in glucose and cholesterol levels after treatment with dexamethasone 4mglkgBw. I/M seen increase in counts of RBCs , PCV, WBCs and differential lenkocyte count and decrease in glucose with cholesterol parameters, histological changes show change in liver after treatment by dexamethasone 4mglKg.Bw ,spleen tissue seen necrosis and pigmentation with hemorrhage after take dexamethasone 4mglkg in (dexamethasone + zinc group). Results also showed that zinc enhanced the immune system in at normal dose for limited time because of its effect on other mineral such as copper and causes anemia , while the dexamethasone is a drug used for antianflammatory but for a short time.

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Direct freezing of whole blood enables analysis of leucocyte markers by flow cytometry: a proof-of-concept study

Future Microbiology ◽

10.2217/fmb-2021-0034 ◽

2021 ◽

Author(s):

Pénélope Bourgoin ◽

Inès Ait Belkacem ◽

Isabelle Arnoux ◽

Pierre-Emmanuel Morange ◽

Fabrice Malergue

Keyword(s):

Flow Cytometry ◽

Whole Blood ◽

Blood Cells ◽

White Blood Cells ◽

Proof Of Concept ◽

Fresh Blood ◽

Blood Samples ◽

Step Flow ◽

Freezing Method ◽

One Step

Aim: A new one-step flow cytometry procedure has been recently demonstrated for identifying subjects with infections, but only for fresh whole blood samples. The goal of this study was to assess its applicability on frozen samples, by proposing a new method to perform the sample freezing directly and easily. Methods: Fresh blood was tested, then frozen either directly or with dimethylsulfoxide and serum. Common markers of white blood cells as well as infection-related biomarkers were tested. Results: All percentages of leucocyte subsets and levels of infection-related biomarkers were significantly correlated between frozen and fresh samples. Conclusion: The direct freezing method enables an accurate assessment of common cellular sub-populations and of levels of important infectious biomarkers via flow cytometry.

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