Streptococci Dominate the Diverse Flora within Buccal Cells

2005 ◽  
Vol 84 (12) ◽  
pp. 1165-1171 ◽  
Author(s):  
J.D. Rudney ◽  
R. Chen ◽  
G. Zhang
Keyword(s):  
Epithelial Cells ◽  
Human Subjects ◽  
Fusobacterium Nucleatum ◽  
Common Species ◽  
Buccal Cells ◽  
Double Labeling ◽  
Buccal Epithelial Cells ◽  
Oral Biofilms ◽  
Granulicatella Adiacens ◽  
Species Specific

Previously, we reported that intracellular Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. In this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify Fusobacterium nucleatum, Prevotella intermedia, oral Campylobacter species, Eikenella corrodens, Treponema denticola, Gemella haemolysans, Granulicatella adiacens, and total streptococci within buccal epithelial cells. All those species were found within buccal cells. In every case, species recognized by green-labeled species-specific probes were accompanied by other bacteria recognized only by a red-labeled universal probe. Streptococci appeared to be a major component of the polymicrobial intracellular flora, being present at a level from one to two logs greater than the next most common species ( G. adiacens). This is similar to what is observed in oral biofilms, where diverse species interact in complex communities that often are dominated by streptococci.

scholarly journals Micronucleus Test in Exfoliated Buccal Cells of Barbecue Grillers in Marawi City, Philippines

2019 ◽  
pp. 33-37
Author(s):  
Abdul Baari’ M. Guma-os ◽  
Annabella G. Villarino
Keyword(s):  
Epithelial Cells ◽  
Micronucleus Test ◽  
Exposed Group ◽  
Office Workers ◽  
State University ◽  
Buccal Cells ◽  
Drinking Habits ◽  
Buccal Epithelial Cells ◽  
Polycyclic Aromatic ◽  
Exfoliated Buccal Cells

Exposure to fumes when grilling meat predisposes human to a significant level of cancer-causing compounds called PAHs (Polycyclic Aromatic Hydrocarbons. The DNA damaging capacity of PAHs can be rapidly and inexpensively evaluated by measuring and counting the micronuclei in various cells. In this study, the frequency of micronucleation (MN) in exfoliated buccal epithelial cells of thirty (N=30) barbecue grillers (exposed group) in Marawi City was compared with thirty (N=30) office workers and students of Mindanao State University (control). A total of 1000 buccal epithelial cells per individual were scored for MN frequency. Results revealed a significant increase (p<0.05) in the MN frequency of barbecue grillers (18.97±3.77) compared with the control (12.6±3.58). In addition, possible effect of the established confounders which include smoking, drinking habits, age, gender and number of years of exposure to PAHs on the frequency of micronucleation was further analysed. Confounding factors that could have caused higher MN frequency in the exposed group are age (ρ=0.000) and length of exposure to grilling fumes (P=0.002). The current study confirms that chronic exposure to grilling fumes increases micronucleation, hence the necessity of biological monitoring and appropriate health interventions.

scholarly journals DNA damage in buccal cells in oral PMDs and malignant disorders by comet assay

2019 ◽  
Vol 18 ◽  
pp. e191430
Author(s):  
Garima Rawat ◽  
Aadithya B Urs ◽  
Anita Chakravarti ◽  
Priya Kumar
Keyword(s):  
Dna Damage ◽  
Epithelial Cells ◽  
Comet Assay ◽  
Oral Submucous Fibrosis ◽  
Tail Length ◽  
Buccal Cells ◽  
Buccal Epithelial Cells ◽  
Significant Difference ◽  
Potentially Malignant ◽  
The Mean

Aim: DNA damage associated with Oral Squamous Cell Carcinoma (OSCC) and potentially malignant disorders (PMDs) is produced due to carcinogenic agents or increased oxidative stress. Comet assay can assist in early detection and evaluation of the amount of DNA damage; lymphocytesare the most commonly used cells for performing comet assay. Utilisation of buccal epithelial cells in comet assay can be a minimally invasive and rapid method.  The present study compared the efficacy of comet assay in assessing DNA damage in buccal cells over peripheral blood leucocytes (PBLs) in oral potentially malignant and malignant disorders. Methods: The study included fifty five patients each of Leukoplakia, Oral Submucous Fibrosis (OSMF) and OSCC along with fifty five healthy individuals as control. Buccal epithelial cells were collected from all the selected subjects. DNA damage was evaluated bymeasuring the mean tail length (µm). Results: A significantly increased mean tail length (µm) and higher DNA damage were found in OSCC (26.1096 + 1.84355) and there was a progressive stepwise increase in mean tail length from control(8.4982 + 0.93307) to PMD [leukoplakia (14.6105 + 0.71857); OSMF (12.5009 + 1.12694)] to OSCC.The mean tail length in different habit groups was greater than controls, though no significant difference was noted between habit groups. The mean tail length of buccal cells was significantly greater than the mean tail length of PBLs in all study groups and controls. Conclusion: Hence, use of comet assay on buccal epithelial cells can prove to be beneficiary for evaluation of DNA damage.

scholarly journals Occurrence of the nan1 gene and adhesion of Pseudomonas aeruginosa isolates to human buccal epithelial cells

2012 ◽  
Vol 49 (1) ◽  
pp. 59-64
Author(s):  
Katarzyna Wolska ◽  
Barbara Kot ◽  
Halina Mioduszewska ◽  
Cezary Sempruch ◽  
Lidia Borkowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Buccal Cells ◽  
Gene Encoding ◽  
Buccal Epithelial Cells ◽  
The Mean ◽  
The Difference

Abstract This study shows an association between the frequency of the nan1 gene (encoding neuraminidase) among 62 clinical Pseudomonas aeruginosa isolates and adhesion of these bacteria to human buccal epithelial cells. The 52 strains in which the gene was present (83.9%) were characterized by a higher adhesiveness (the mean number of adhering bacteria was 23.51 per cell) than strains in which the gene was not detected (16.23 per cell) and the difference was significant (P = 0.009, Mann-Whitney U test). Thus we found that the nan1 gene may play a role in the binding of clinical P. aeruginosa strains to buccal cells.

scholarly journals Intracellular Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in Buccal Epithelial Cells Collected from Human Subjects

2001 ◽  
Vol 69 (4) ◽  
pp. 2700-2707 ◽  
Author(s):  
Joel D. Rudney ◽  
Ruoqiong Chen ◽  
Gerald J. Sedgewick
Keyword(s):  
Epithelial Cells ◽  
Porphyromonas Gingivalis ◽  
Laser Scanning ◽  
Human Subjects ◽  
Laser Scanning Confocal Microscopy ◽  
Actinobacillus Actinomycetemcomitans ◽  
Periodontal Pathogens ◽  
Scanning Confocal Microscopy ◽  
Buccal Epithelial Cells

ABSTRACT The mouth may provide an accessible model for studying bacterial interactions with human cells in vivo. Using fluorescent in situ hybridization and laser scanning confocal microscopy, we found that human buccal epithelial cells from 23 of 24 subjects were infected with intracellular bacteria, including the periodontal pathogens Actinobacillus actinomycetemcomitans andPorphyromonas gingivalis, as well as other species which have yet to be identified. Buccal cell invasion may allow fastidious anaerobes to establish themselves in aerobic sites that otherwise present an unfavorable environment. Exfoliated buccal epithelial cells might provide a protected route for bacterial transmission between different oral sites within and between hosts.

scholarly journals Characterization of the adherence properties of Streptococcus salivarius

1980 ◽  
Vol 29 (2) ◽  
pp. 459-468 ◽  
Author(s):  
A H Weerkamp ◽  
B C McBride
Keyword(s):  
Epithelial Cells ◽  
Divalent Cations ◽  
Sodium Dodecyl ◽  
Fusobacterium Nucleatum ◽  
Human Saliva ◽  
Human Erythrocytes ◽  
The Other ◽  
Streptococcus Salivarius ◽  
Streptococcus Group ◽  
Buccal Epithelial Cells

The adherence and aggregation properties of 46 human oral Streptococcus salivarius isolates were examined. A total of 41% of the isolates aggregated with whole human saliva, 50% aggregated with human erythrocytes, and 85% adhered to human buccal epithelial cells. Strains that aggregated with saliva and erythrocytes usually reacted with Streptococcus group K typing serum whereas the non-hemagglutinating strains did not. K+ strains also adhered more strongly to human buccal epithelial cells than K- strains. All isolates coaggregated with Fusobacterium nucleatum LF and Bacteroides asaccharolyticus 2D, 91% coaggregated with Veillonella alcalescens V1, and 50% coaggregated with Veillonella parvula V4. S. salivarius HB aggregated with saliva from 15 different human donors and aggregated with human erythrocytes irrespective of the blood group. This strain only weakly aggregated with rat saliva or rat erythrocytes. We isolated mutants which concomitantly lost the ability to agglutinate erythrocytes, aggregate with saliva, and bind to buccal epithelial cells, but retained their interbacterial aggregation properties. A second class of mutants lost the ability to coaggregate with Veillonella, but these mutants retained all of the other aggregation properties. Treatment of S. salivarius HB cells with pronase or subtilisin destroyed their ability to aggregate with saliva and erythrocytes and to bind to buccal epithelial cells. The unique characteristics of the aggregation and adherence reactions were suggested by differences in the rate of loss of activity during protease treatment and in the response to chemical modification. The presence of saliva did not affect hemagglutination and adherence to buccal epithelial cells. Binding of the salivary aggregating factor to the bacteria could be distinguished from aggregation on the basis that the latter required divalent cations. The factor involved in coaggregation with F. nucleatum LF was physicochemically different from the other factors, since it was resistant to heat and to extraction with trichloroacetic acid, aqueous phenol, sodium dodecyl sulfate, and formamide, but was sensitive to proteases and was present in both classes of mutants. Coaggregation with V. alcalescens was not sensitive to proteases. A variety of mono- and disaccharides had no influence on any of the reactions tested.

scholarly journals A multi-bioassay integrated approach to assess antifouling potential of extracts from the Mediterranean sponge Ircinia oros

2021 ◽  
Author(s):  
Lucia De Marchi ◽  
Carlo Pretti ◽  
Alessia Cuccaro ◽  
Matteo Oliva ◽  
Federica Tardelli ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Integrated Approach ◽  
Common Species ◽  
Marine Diatom ◽  
Cellular Damage ◽  
Inhibition Of Growth ◽  
Aliivibrio Fischeri ◽  
The Mediterranean ◽  
Ircinia Oros ◽  
Species Specific

AbstractThe phylum Porifera and their symbionts produce a wide variety of bioactive compounds, playing a central role in their ecology and evolution. In this study, four different extracts (obtained by non-polar and semi-polar extraction methodologies) of the Mediterranean sponge Ircinia oros were tested through a multi-bioassay integrated approach to assess their antifouling potential. Tests were performed using three common species, associated with three different endpoints: the marine bacterium Aliivibrio fischeri (inhibition of bioluminescence), the marine diatom Phaeodactylum tricornutum (inhibition of growth), and different development stages of the brackish water serpulid Ficopomatus enigmaticus (gametes: sperm motion, vitality inhibition and cellular damage; larvae: development; adults: AChE (acetylcholinesterase)-inhibitory activity). The effects of extracts were species specific and did not vary among different extraction methodologies. In particular, no significant reduction of bioluminescence of A. fischeri was observed for all tested samples. By contrast, extracts inhibited P. tricornutum growth and had toxic effects on different F. enigmaticus’ developmental stages. Our results suggest that the proposed test battery can be considered a suitable tool as bioactivity screening of marine natural products.

Inhibition of microbial adherence by sphinganine

1992 ◽  
Vol 38 (9) ◽  
pp. 983-985 ◽  
Author(s):  
Debra Jan Bibel ◽  
Raza Aly ◽  
Henry R. Shinefield
Keyword(s):  
Staphylococcus Aureus ◽  
Infectious Diseases ◽  
Therapeutic Agents ◽  
Eukaryotic Cells ◽  
Buccal Cells ◽  
Streptococcus Mitis ◽  
Buccal Epithelial Cells ◽  
Mucosal Cells ◽  
Microbial Adherence ◽  
Complex Sphingolipids

Sphingosines (precursors and degeneration products of complex sphingolipids) are mediators in membrane second-messenger cascades and in a wide variety of functions in eukaryotic cells. Sphingosines are also lethal for gram-positive microorganisms. In addition to its direct effect, sphinganine is here reported to affect the adherence of Streptococcus mitis to buccal epithelial cells and of Staphylococcus aureus to nasal mucosal cells after incubation for 90 min at 37 °C. When the bacteria were pretreated with 8.1, 16.2, 32.5, or (for Strep. mitis) 65 μM sphinganine for 60 min at 37 °C, adherence counts were reduced for Staph. aureus by 27, 37, and 60% and for Strep. mitis by 19, 44, 54, and 73%, respectively (p < 0.001). In contrast, pretreatment of buccal cells with 81.2 μM lipid increased adherence by 14% (p < 0.01), but no change occurred at either 16.2 or 325 μM lipid. These results further demonstrate the double-edged ability of sphingosines to regulate cellular activities and their potential as multifunctional therapeutic agents for infectious diseases. Key words: adherence, sphingosine, Staphylococcus aureus, Streptococcus mitis.

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