scholarly journals Occurrence of the nan1 gene and adhesion of Pseudomonas aeruginosa isolates to human buccal epithelial cells

2012 ◽  
Vol 49 (1) ◽  
pp. 59-64
Author(s):  
Katarzyna Wolska ◽  
Barbara Kot ◽  
Halina Mioduszewska ◽  
Cezary Sempruch ◽  
Lidia Borkowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Buccal Cells ◽  
Gene Encoding ◽  
Buccal Epithelial Cells ◽  
The Mean ◽  
The Difference

Abstract This study shows an association between the frequency of the nan1 gene (encoding neuraminidase) among 62 clinical Pseudomonas aeruginosa isolates and adhesion of these bacteria to human buccal epithelial cells. The 52 strains in which the gene was present (83.9%) were characterized by a higher adhesiveness (the mean number of adhering bacteria was 23.51 per cell) than strains in which the gene was not detected (16.23 per cell) and the difference was significant (P = 0.009, Mann-Whitney U test). Thus we found that the nan1 gene may play a role in the binding of clinical P. aeruginosa strains to buccal cells.

scholarly journals DNA damage in buccal cells in oral PMDs and malignant disorders by comet assay

2019 ◽  
Vol 18 ◽  
pp. e191430
Author(s):  
Garima Rawat ◽  
Aadithya B Urs ◽  
Anita Chakravarti ◽  
Priya Kumar
Keyword(s):  
Dna Damage ◽  
Epithelial Cells ◽  
Comet Assay ◽  
Oral Submucous Fibrosis ◽  
Tail Length ◽  
Buccal Cells ◽  
Buccal Epithelial Cells ◽  
Significant Difference ◽  
Potentially Malignant ◽  
The Mean

Aim: DNA damage associated with Oral Squamous Cell Carcinoma (OSCC) and potentially malignant disorders (PMDs) is produced due to carcinogenic agents or increased oxidative stress. Comet assay can assist in early detection and evaluation of the amount of DNA damage; lymphocytesare the most commonly used cells for performing comet assay. Utilisation of buccal epithelial cells in comet assay can be a minimally invasive and rapid method.  The present study compared the efficacy of comet assay in assessing DNA damage in buccal cells over peripheral blood leucocytes (PBLs) in oral potentially malignant and malignant disorders. Methods: The study included fifty five patients each of Leukoplakia, Oral Submucous Fibrosis (OSMF) and OSCC along with fifty five healthy individuals as control. Buccal epithelial cells were collected from all the selected subjects. DNA damage was evaluated bymeasuring the mean tail length (µm). Results: A significantly increased mean tail length (µm) and higher DNA damage were found in OSCC (26.1096 + 1.84355) and there was a progressive stepwise increase in mean tail length from control(8.4982 + 0.93307) to PMD [leukoplakia (14.6105 + 0.71857); OSMF (12.5009 + 1.12694)] to OSCC.The mean tail length in different habit groups was greater than controls, though no significant difference was noted between habit groups. The mean tail length of buccal cells was significantly greater than the mean tail length of PBLs in all study groups and controls. Conclusion: Hence, use of comet assay on buccal epithelial cells can prove to be beneficiary for evaluation of DNA damage.

scholarly journals Carbapenem-Resistant Pseudomonas aeruginosa Strains-Distribution of the Essential Enzymatic Virulence Factors Genes

Antibiotics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 8
Author(s):  
Tomasz Bogiel ◽  
Małgorzata Prażyńska ◽  
Joanna Kwiecińska-Piróg ◽  
Agnieszka Mikucka ◽  
Eugenia Gospodarek-Komkowska
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Alkaline Protease ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Hospital Environment ◽  
Gene Encoding ◽  
Virulence Gene Expression ◽  
Carbapenem Resistant ◽  
Antimicrobial Resistance Genes ◽  
The Difference

Pseudomonas aeruginosa is one of the most commonly isolated bacteria from clinical specimens, with increasing isolation frequency in nosocomial infections. Herein, we investigated whether antimicrobial-resistant P. aeruginosa strains, e.g., metallo-beta-lactamase (MBL)-producing isolates, may possess a reduced number of virulence genes, resulting from appropriate genome management to adapt to a changing hospital environment. Hospital conditions, such as selective pressure, may lead to the replacement of virulence genes by antimicrobial resistance genes that are crucial to survive under current conditions. The study aimed to compare, using PCR, the frequency of the chosen enzymatic virulence factor genes (alkaline protease-aprA, elastase B-lasB, neuraminidases-nan1 and nan2, and both variants of phospholipase C-plcH and plcN) to MBL distribution among 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. The gene encoding alkaline protease was noted with the highest frequency (100%), while the neuraminidase-1 gene was observed in 37.4% of the examined strains. The difference in lasB and nan1 prevalence amongst the MBL-positive and MBL-negative strains, was statistically significant. Although P. aeruginosa virulence is generally more likely determined by the complex regulation of the virulence gene expression, herein, we found differences in the prevalence of various virulence genes in MBL-producers.

Role of pili in adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells

1980 ◽  
Vol 29 (3) ◽  
pp. 1146-1151 ◽  
Author(s):  
D E Woods ◽  
D C Straus ◽  
W G Johanson ◽  
V K Berry ◽  
J A Bass
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Opportunistic Pathogen ◽  
In Vitro System ◽  
Heterologous Antiserum ◽  
Buccal Epithelial Cells ◽  
Serological Studies ◽  
Seriously Ill Patients

Adherence of Pseudomonas aeruginosa organisms to the upper respiratory epithelium of seriously ill patients in vitro is correlated with subsequent colonization of the respiratory tract by this opportunistic pathogen. The role of pili in the attachment to epithelial cells of P. aeruginosa was studied in an in vitro system employing human buccal epithelial cells and P. aeruginosa pretreated by various means. Pretreatment of the bacteria with proteases, heat, or Formalin caused a significant decrease in adherence. A decrease when compared with controls was also noted in the adherence of P. aeruginosa organisms to buccal epithelial cells preincubated with purified pili prepared from the strain used for adherence testing; however, pili prepared from a heterologous strain failed to block adherence. Similar results were obtained in serological studies when antisera to purified pili prepared from the strain used for adherence testing decreased adherence, whereas heterologous antiserum to pili did not decrease adherence. From these results it appears that pili mediate the adherence of P. aeruginosa organisms to human buccal epithelial cells.

Factors Influencing the Adherence of Pseudomonas aeruginosa to Mammalian Buccal Epithelial Cells

1983 ◽  
Vol 5 (Supplement_5) ◽  
pp. S846-S851 ◽  
Author(s):  
Donald E. Woods ◽  
David C. Straus ◽  
W. G. Johanson ◽  
Joe A. Bass
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Factors Influencing ◽  
Buccal Epithelial Cells

The binding of Pseudomonas aeruginosa outer membrane ghosts to human buccal epithelial cells

1986 ◽  
Vol 32 (2) ◽  
pp. 160-166 ◽  
Author(s):  
P. Doig ◽  
A. L. Franklin ◽  
R. T. Irvin
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Outer Membrane ◽  
Copy Number ◽  
Electron Microscopic Examination ◽  
Equilibrium Analysis ◽  
Metabolic Effects ◽  
Electron Microscopic ◽  
Buccal Epithelial Cells ◽  
Number Class

The binding of outer membrane (OM) ghosts derived from Pseudomonas aeruginosa strain 492c to human buccal epithelial cells (BECs) was examined. Electron microscopic examination of the binding of OM ghosts to BECs revealed direct OM ghost–BEC interaction. Equilibrium analysis of the binding of OM ghosts to trypsinized BECs employing the Langmuir adsorption isotherm indicated the number of binding sites (iV) to be 1.3 × 10−1 μg protein per BEC with an apparent association constant (Ka) of 3.4 × 10−2 mL/μg protein. The Langmuir analysis of binding of OM ghosts to untrypsinized BECs was complex, suggesting two possible classes of receptors, a high affinity–low copy number class (Ka, 1.8 × 10−2 mL/μg protein; N, 8.6 × 10−5 μg protein per BEC) and a low affinity – high copy number class(Afa, 3.7 × 10−3 mL/μg protein; N, 9.2 × 10−4 μg protein per BEC). Sugar inhibition studies incorporating D-galactose enhanced binding to each BEC type. N-Acetylneuraminic acid and N-acetyl-glucosamine both enhanced binding of OM ghosts to untrypsinized BECs, while inhibiting binding to trypsinized BECs. D-Arabinose inhibited binding to both BEC types. Binding of OM ghosts to both BEC types was greatly inhibited by D-fucose, while L-fucose only greatly inhibited binding to untrypsinized BECs. These sugar inhibition data demonstrated a difference in the binding of OM ghosts to trypsinized and untrypsinized BECs and possibly reveal the nature of the receptor(s), free of possible bacterial metabolic effects. These data indicated that OM ghosts from 492c appear to bind to BECs in a similar manner to the intact bacteria and represent a simple model system to study the adhesion of P. aeruginosa to BECs.

Streptococci Dominate the Diverse Flora within Buccal Cells

2005 ◽  
Vol 84 (12) ◽  
pp. 1165-1171 ◽  
Author(s):  
J.D. Rudney ◽  
R. Chen ◽  
G. Zhang
Keyword(s):  
Epithelial Cells ◽  
Human Subjects ◽  
Fusobacterium Nucleatum ◽  
Common Species ◽  
Buccal Cells ◽  
Double Labeling ◽  
Buccal Epithelial Cells ◽  
Oral Biofilms ◽  
Granulicatella Adiacens ◽  
Species Specific

Previously, we reported that intracellular Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. In this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify Fusobacterium nucleatum, Prevotella intermedia, oral Campylobacter species, Eikenella corrodens, Treponema denticola, Gemella haemolysans, Granulicatella adiacens, and total streptococci within buccal epithelial cells. All those species were found within buccal cells. In every case, species recognized by green-labeled species-specific probes were accompanied by other bacteria recognized only by a red-labeled universal probe. Streptococci appeared to be a major component of the polymicrobial intracellular flora, being present at a level from one to two logs greater than the next most common species ( G. adiacens). This is similar to what is observed in oral biofilms, where diverse species interact in complex communities that often are dominated by streptococci.

Cytomorphometric and Morphological Analysis in Women with Trichomonas vaginalis Infection: Micronucleus Frequency in Exfoliated Cervical Epithelial Cells

2015 ◽  
Vol 59 (3) ◽  
pp. 258-264 ◽  
Author(s):  
Zehra Safi Oz ◽  
Banu Doğan Gun ◽  
Mustafa Ozkan Gun ◽  
Sukru Oguz Ozdamar
Keyword(s):  
Epithelial Cells ◽  
Trichomonas Vaginalis ◽  
Control Group ◽  
Study Group ◽  
Control Groups ◽  
The Mean ◽  
The Difference ◽  
Group 2 ◽  
And Control ◽  
Group 1

Objectives: The aim of this study was to explore the cytomorphometric and morphological effects of Trichomonas vaginalis in exfoliated epithelial cells. Study Design: Ninety-six Pap-stained cervical smears were divided into a study group and two control groups as follows: T. vaginalis cases, a first control group with inflammation, and a second control group without inflammation. Micronucleated, binucleated, karyorrhectic, karyolytic, and karyopyknotic cells and cells with perinuclear halos per 1,000 epithelial cells were counted. Nuclear and cellular areas were evaluated in 70 clearly defined cells in each smear using image analysis. Results: The frequencies of morphological parameters in the T. vaginalis cases were higher than the values of the two control groups, and the difference among groups was found to be significant (p < 0.05). The nuclear and cytoplasmic areas of epithelial cells were diminished in patients with trichomoniasis. The mean nucleus/cytoplasm ratio in T. vaginalis patients was higher than the value in the control groups, and the difference between the study group and control group 1 was significant. However, there was no statistically significant increase between the study group and control group 2. Conclusions:T. vaginalis exhibited significant changes in the cellular size and nuclear structure of the cells. The rising frequency of micronuclei, nuclear abnormalities, and changing nucleus/cytoplasm ratio may reflect genotoxic damage in trichomoniasis.

Analysis and modelling of Pseudomonas aeruginosa adherence to human buccal epithelial cells

1997 ◽  
Vol 8 (4-5) ◽  
pp. 267-277
Author(s):  
Anne M.C. Cerf ◽  
Jean-Paul Dehaye ◽  
Michel J. Devleeschouwer
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Buccal Epithelial Cells

scholarly journals Evaluation of Disinfection Quality of Dental Faculty Units of Ahvaz Jundishapur University of Medical Sciences, Southwest of Iran in 2017

2018 ◽  
Vol 8 (1) ◽  
pp. 88
Author(s):  
Seyed Morteza Bagheri ◽  
Mohammad Shooriabi ◽  
Mansour Amin ◽  
Fatemeh Babadi ◽  
Asadollah Ahmadzadeh ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Medical Sciences ◽  
Oral Environment ◽  
Before And After ◽  
Dental Practices ◽  
The Mean ◽  
The Difference ◽  
Southwest Of Iran

Introduction: Performing dental practices in the oral environment leads to the transmission of microorganisms in saliva and blood to working surfaces and dental devices and their infection. Preventing transmission of infection through these devices is an important task of a dentist. Hence, this research was conducted to evaluate the disinfection quality of the dental faculty units of Ahvaz Jundishapur University of Medical Sciences (AJUMS) in Iran.Materials and Methods: In order to evaluate the quality of disinfection of the units, sampling was performed from all glasses spittoon surfaces of 90 units of the clinical unit of the AJUMS dental faculty before and after disinfection by personnel. Then, the bacteria were cultured in a medium and examined.Results: The mean (and standard deviation) of the total infection of units of the dental faculty was 46534.4 (583380.4) colonies per 1 ml before disinfection and 40265.6 (52131.1) colonies per 1 ml after disinfection, reflecting significant decrease in number of bacterial colonies after disinfection (P &lt;0.001). In addition, a significant decrease was seen in the number of bacterial colonies in the restoration, pediatric, orthodontic and diagnosis units (p &lt;0.05), but the difference before and after disinfection was not significant in the prosthetic, endodontic, surgical and periodontal units. In addition, the most common types of microorganisms in the whole units of the dental faculty were pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus, respectively, and after disinfection, the most common types of microorganisms were Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus, respectively.Conclusion: In general, this study showed that the disinfection method of units in dental faculty can not reduce the severity of infection of the units. Given what was stated, it is recommended that the method and the substances used to disinfect the unit to be changed.

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