scholarly journals Micronucleus Test in Exfoliated Buccal Cells of Barbecue Grillers in Marawi City, Philippines

2019 ◽  
pp. 33-37
Author(s):  
Abdul Baari’ M. Guma-os ◽  
Annabella G. Villarino
Keyword(s):  
Epithelial Cells ◽  
Micronucleus Test ◽  
Exposed Group ◽  
Office Workers ◽  
State University ◽  
Buccal Cells ◽  
Drinking Habits ◽  
Buccal Epithelial Cells ◽  
Polycyclic Aromatic ◽  
Exfoliated Buccal Cells

Exposure to fumes when grilling meat predisposes human to a significant level of cancer-causing compounds called PAHs (Polycyclic Aromatic Hydrocarbons. The DNA damaging capacity of PAHs can be rapidly and inexpensively evaluated by measuring and counting the micronuclei in various cells. In this study, the frequency of micronucleation (MN) in exfoliated buccal epithelial cells of thirty (N=30) barbecue grillers (exposed group) in Marawi City was compared with thirty (N=30) office workers and students of Mindanao State University (control). A total of 1000 buccal epithelial cells per individual were scored for MN frequency. Results revealed a significant increase (p<0.05) in the MN frequency of barbecue grillers (18.97±3.77) compared with the control (12.6±3.58). In addition, possible effect of the established confounders which include smoking, drinking habits, age, gender and number of years of exposure to PAHs on the frequency of micronucleation was further analysed. Confounding factors that could have caused higher MN frequency in the exposed group are age (ρ=0.000) and length of exposure to grilling fumes (P=0.002). The current study confirms that chronic exposure to grilling fumes increases micronucleation, hence the necessity of biological monitoring and appropriate health interventions.

Streptococci Dominate the Diverse Flora within Buccal Cells

2005 ◽  
Vol 84 (12) ◽  
pp. 1165-1171 ◽  
Author(s):  
J.D. Rudney ◽  
R. Chen ◽  
G. Zhang
Keyword(s):  
Epithelial Cells ◽  
Human Subjects ◽  
Fusobacterium Nucleatum ◽  
Common Species ◽  
Buccal Cells ◽  
Double Labeling ◽  
Buccal Epithelial Cells ◽  
Oral Biofilms ◽  
Granulicatella Adiacens ◽  
Species Specific

Previously, we reported that intracellular Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. In this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify Fusobacterium nucleatum, Prevotella intermedia, oral Campylobacter species, Eikenella corrodens, Treponema denticola, Gemella haemolysans, Granulicatella adiacens, and total streptococci within buccal epithelial cells. All those species were found within buccal cells. In every case, species recognized by green-labeled species-specific probes were accompanied by other bacteria recognized only by a red-labeled universal probe. Streptococci appeared to be a major component of the polymicrobial intracellular flora, being present at a level from one to two logs greater than the next most common species ( G. adiacens). This is similar to what is observed in oral biofilms, where diverse species interact in complex communities that often are dominated by streptococci.

Clinical application of micronucleus test in exfoliated buccal cells: A systematic review and metanalysis

2015 ◽  
Vol 766 ◽  
pp. 20-31 ◽  
Author(s):  
Claudia Bolognesi ◽  
Stefano Bonassi ◽  
Siegfried Knasmueller ◽  
Michael Fenech ◽  
Marco Bruzzone ◽  
...  
Keyword(s):  
Systematic Review ◽  
Clinical Application ◽  
Micronucleus Test ◽  
Buccal Cells ◽  
Exfoliated Buccal Cells

scholarly journals Electrophoretic Mobility of Red Blood Cells and Micronucleus Test in Exfoliated Buccal Cells as Stress Intensity Markers

2018 ◽  
Vol 8 (4) ◽  
pp. 347-350 ◽  
Author(s):  
Anna Deryugina ◽  
Marina Ivashchenko ◽  
Pavel Ignatyev ◽  
Marina Zolotova ◽  
Аleksandr Samodelkin
Keyword(s):  
Stress Intensity ◽  
Red Blood Cells ◽  
Electrophoretic Mobility ◽  
Blood Cells ◽  
Micronucleus Test ◽  
Buccal Cells ◽  
As Stress ◽  
Exfoliated Buccal Cells

scholarly journals INFLUENCE OF SPELEOCLIMATOTHERAPY ON THE PSYCHO-EMOTIONAL STATE, GENETIC STABILITY AND ACTIVITY OF α-AMYLASE IN THE ORAL CAVITY OF THE STUDENTS

2020 ◽  
Vol 74 (2) ◽  
pp. 139-144
Author(s):  
M.S. Nechaeva ◽  
◽  
O. I. Tyunina ◽  
E.V. Dorokhov ◽  
A.Yu. Aralova ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Oral Cavity ◽  
Genetic Stability ◽  
Emotional State ◽  
Solid Phase ◽  
Amylase Activity ◽  
Micronucleus Test ◽  
Buccal Epithelium ◽  
Level Of Activity ◽  
Buccal Epithelial Cells

The goal of investigation was to evaluate the effect of speleoclimate on the psycho-emotional state, the frequency of occurrence of buccal epithelial cells with core aberrations and the level of α-amylase activity in the oral cavity of students. Second-year students of VSMU named N. N. Burdenko were selected as subjects. The collection of psychological parameters, buccal epithelium, to assess genetic stability, and oral fluid, for evaluation of the activity of α-amylase was carried out in the first, fourth, eighth, eleventh day visit speleochamber and four days after a course of speleoclimate. The psycho-emotional state of the subjects was assessed using the SAN questionnaire and the scale of C.D. Spielberger and Y.L. Khanin. The state of genetic homeostasis of students was evaluated using a micronucleus test in the buccal epithelium. The level of activity of α-amylase was carried out by the method of solid-phase enzyme immunoassay. Comparison of the studied indicators was carried out using the nonparametric Van-der-Warden criterion. The relationship between the dynamics of α-amylase activity and cytological parameters was revealed using the Spearman correlation coefficient (r s ). The results obtained indicate a positive effect of speleoclimate on the psychological and genetic stability of a person. Subjects with low α-amylase activity showed a higher level of cells with abnormal nuclei. A negative correlation was found between the dynamics of the frequency of abnormal nuclei in buccal epithelial cells and the activity of α-amylase in the group with initially low activity (r s = – 0,8; P < 0,05). Speleoclimatotherapy can be recommended as a method of non-medicamental correction of the effects of stress influence on health of students.

scholarly journals Micronucleus evaluation of exfoliated buccal epithelial cells from automobile spray painters – A preliminary study

2019 ◽  
Vol 8 (1) ◽  
pp. 1460-1469
Author(s):  
Nkiruka Chinonyelum Azubuike ◽  
Okechukwu Steven Onwukwe ◽  
Anulika Obianuju Onyemelukwe ◽  
Uzoamaka Charity Maduakor ◽  
Iniekong Philip Udoh ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Epithelial Cells ◽  
Working Environment ◽  
Control Group ◽  
Buccal Epithelial Cells ◽  
Genotoxic Agents ◽  
Mucosal Cells ◽  
Enugu State ◽  
Study Participants ◽  
Exfoliated Buccal Cells

Automobile spray painters in the Mechanic village at Coal Camp, Enugu State, Nigeria are exposed to genotoxic agents such as the automobile paints and spent engine lubricants which contaminate the soil of their working environment. The present study applied the micronucleus (MN) test on exfoliated oral mucosal cells as a means to assess the genotoxicity risk associated with occupational exposure to genotoxic agents. Two (2) groups of subjects which comprised 30 automobile spray painters and a control group of 30 apparently healthy unexposed volunteers were enrolled in the study. The study participants were all males. Moistened wooden spatulas were used to obtain buccal smears of the participating individuals, and the smears were stained. The presence of MN was assessed under light  microscopy and a total of 1000 cells per individual were scored. The results obtained showed that statistically significant increase in MN frequency in buccal epithelial cells of automobile spray painters when compared with the control group (p<0.05). Elevated MN frequency was also observed withincreased age, smoking and alcohol consumption habits. MN frequency was significantly affected by the duration of working experience (years) of the spray painters whereas no difference was observed with number of workinghours/day. In conclusion, the present study has revealed that automobile spray painters in the Mechanic village of Coal Camp, Enugu State could be under risk of cytogenetic damage from exposure to genotoxic chemicals.Key words: Micronuclei frequency; Exfoliated buccal cells; Smoking;  Alcohol consumption, Spray Painters; Genotoxicity;

scholarly journals DNA damage in buccal cells in oral PMDs and malignant disorders by comet assay

2019 ◽  
Vol 18 ◽  
pp. e191430
Author(s):  
Garima Rawat ◽  
Aadithya B Urs ◽  
Anita Chakravarti ◽  
Priya Kumar
Keyword(s):  
Dna Damage ◽  
Epithelial Cells ◽  
Comet Assay ◽  
Oral Submucous Fibrosis ◽  
Tail Length ◽  
Buccal Cells ◽  
Buccal Epithelial Cells ◽  
Significant Difference ◽  
Potentially Malignant ◽  
The Mean

Aim: DNA damage associated with Oral Squamous Cell Carcinoma (OSCC) and potentially malignant disorders (PMDs) is produced due to carcinogenic agents or increased oxidative stress. Comet assay can assist in early detection and evaluation of the amount of DNA damage; lymphocytesare the most commonly used cells for performing comet assay. Utilisation of buccal epithelial cells in comet assay can be a minimally invasive and rapid method.  The present study compared the efficacy of comet assay in assessing DNA damage in buccal cells over peripheral blood leucocytes (PBLs) in oral potentially malignant and malignant disorders. Methods: The study included fifty five patients each of Leukoplakia, Oral Submucous Fibrosis (OSMF) and OSCC along with fifty five healthy individuals as control. Buccal epithelial cells were collected from all the selected subjects. DNA damage was evaluated bymeasuring the mean tail length (µm). Results: A significantly increased mean tail length (µm) and higher DNA damage were found in OSCC (26.1096 + 1.84355) and there was a progressive stepwise increase in mean tail length from control(8.4982 + 0.93307) to PMD [leukoplakia (14.6105 + 0.71857); OSMF (12.5009 + 1.12694)] to OSCC.The mean tail length in different habit groups was greater than controls, though no significant difference was noted between habit groups. The mean tail length of buccal cells was significantly greater than the mean tail length of PBLs in all study groups and controls. Conclusion: Hence, use of comet assay on buccal epithelial cells can prove to be beneficiary for evaluation of DNA damage.

scholarly journals Occurrence of the nan1 gene and adhesion of Pseudomonas aeruginosa isolates to human buccal epithelial cells

2012 ◽  
Vol 49 (1) ◽  
pp. 59-64
Author(s):  
Katarzyna Wolska ◽  
Barbara Kot ◽  
Halina Mioduszewska ◽  
Cezary Sempruch ◽  
Lidia Borkowska ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Buccal Cells ◽  
Gene Encoding ◽  
Buccal Epithelial Cells ◽  
The Mean ◽  
The Difference

Abstract This study shows an association between the frequency of the nan1 gene (encoding neuraminidase) among 62 clinical Pseudomonas aeruginosa isolates and adhesion of these bacteria to human buccal epithelial cells. The 52 strains in which the gene was present (83.9%) were characterized by a higher adhesiveness (the mean number of adhering bacteria was 23.51 per cell) than strains in which the gene was not detected (16.23 per cell) and the difference was significant (P = 0.009, Mann-Whitney U test). Thus we found that the nan1 gene may play a role in the binding of clinical P. aeruginosa strains to buccal cells.

Sign in / Sign up

Export Citation Format

Share Document