Anti-inflammatory effects of 1-isothiocyanato-7-(methylsulfonyl) heptane by suppressing the NFκ-B signaling pathway

The anti-inflammatory roles of I7456 (1-isothiocyanato-7-(methylsulfonyl) heptane or 7-methylsulfonylheptyl isothiocyanate), a plant-derived and sulfur-containing isothiocyanate, were investigated. When macrophage cells (RAW 264.7) were challenged with lipopolysaccharide (LPS), nitric oxide (NO) increased. However, NO was remarkably reduced upon I7456 treatment. I7456 strongly reduced the expression of IL-6, IL-10, IL-1β, and iNOS. Interestingly, heme oxygenase-1 (HO-1) was strongly induced without LPS challenge. LPS-induced NFκ-B (nuclear factor kappa-light-chain-enhancer of activated B cells) translocation into the nucleus was inhibited by I7456 in a dose-dependent manner. I7456 markedly reduced the phosphorylation level of IκB, and NFκ-B remained inactivated. I7456 could play important roles in anti-inflammatory responses and have implications for anticancer treatments.

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Compound Traditional Chinese Medicine Dermatitis Ointment Ameliorates Inflammatory Responses and Dysregulation of Itch-Related Molecules in AD

10.21203/rs.3.rs-783506/v1 ◽

2021 ◽

Author(s):

Rongrong Zhang ◽

Shuai Shao ◽

Yingxin Shen ◽

Jiaming Sun ◽

Songlan Piao ◽

...

Keyword(s):

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Inflammatory Responses ◽

Dependent Manner ◽

Inflammatory Skin Disease ◽

Fingerprint Analysis ◽

Hplc Fingerprint ◽

Effective Components ◽

Anti Inflammatory ◽

Dose Dependent

Abstract Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease accompanied by an itchy and scaly rash. Compound traditional Chinese medicine dermatitis ointment (CTCMDO) is a traditional classics aimed at AD composed of a mixture of extracts from five plants known to have anti-inflammatory and antiallergic effects. Materials and methods: In this study, we used HPLC and LC/MS to analyze the effective components of CTCMDO in detail and establish its HPLC fingerprint analysis. On this basis, this article studied the anti-inflammatory and antipruritic activities of CTCMDO in the treatment of DNCB-induced AD in mice.Results: Through comparison with literature data, a total of 43 compounds were identified, including phenylpropionic acid compounds; alkaloid compounds; curcumin compounds and lignans. On this basis, a fingerprint with 17 common peaks was established. In AD-like mice, CTCMDO treatment suppressed the scratching behavior induced by DNCB in a dose-dependent manner and inhibited the production of Th1/2 cytokines in serum. CTCMDO treatment reversed the up regulation of P substance levels of itch-related genes in the skin. Furthermore, CTCMDO suppressed the phosphorylation of JNK、ERK and p38 in the skin. Conclusion: In all, our work indicated that CTCMDO can signifificantly attenuate the pathological alterations of Th1/2 cytokines and itch-related mediators, and inhibit the phosphorylation of MAPKs to treat AD.

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Therapeutic effect and mechanism of action of quercetin in a rat model of osteoarthritis

Journal of International Medical Research ◽

10.1177/0300060519873461 ◽

2019 ◽

Vol 48 (3) ◽

pp. 030006051987346 ◽

Cited By ~ 1

Author(s):

Jun Zhang ◽

Jing Yin ◽

Daohong Zhao ◽

Chaoran Wang ◽

Yuhao Zhang ◽

...

Keyword(s):

Therapeutic Effect ◽

Mechanism Of Action ◽

Rat Model ◽

Dependent Manner ◽

Nuclear Factor ◽

Toll Like Receptor ◽

Tnf Α ◽

Dose Dependent ◽

Light Chain Enhancer ◽

Necrosis Factor

Objective To study the therapeutic effect and mechanism of action of quercetin in a rat model of osteoarthritis (OA). Methods The OA rat model was established by intra-articular injection of papain. Changes in knee diameter, toe volume and histopathology were measured. Levels of interleukin (IL)-β and tumor necrosis factor (TNF)-α were assessed by ELISA. Relative expression of Toll-like receptor (TLR)-4 and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) was evaluated by western blotting. Results Compared with rats treated with papain alone, changes in knee diameter, toe volume and Makin' s score were less apparent in OA rats treated with quercetin. Levels of serum IL-1β and TNF-α were also reduced in quercetin-treated OA rats. Expression of TLR-4 and NF-κB was significantly suppressed in a dose-dependent manner in quercetin-treated OA rats. Conclusion Quercetin exhibited a therapeutic effect in OA rats, which may be related to inhibition of IL-1β and TNF-α production via the TLR-4/NF-κB pathway.

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Antineuroinflammatory and Neuroprotective Effects of Gyejibokryeong-Hwan in Lipopolysaccharide-Stimulated BV2 Microglia

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/7585896 ◽

2019 ◽

Vol 2019 ◽

pp. 1-14 ◽

Cited By ~ 6

Author(s):

Bo-Kyung Park ◽

Young Hwa Kim ◽

Yu Ri Kim ◽

Jeong June Choi ◽

Changsop Yang ◽

...

Keyword(s):

Nitric Oxide ◽

Mitogen Activated Protein Kinase ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Dependent Manner ◽

Nuclear Factor ◽

Neuroprotective Effects ◽

Bv2 Cells ◽

Bv2 Microglia ◽

Anti Inflammatory

Microglia, the central nervous system’s innate immune cells, mediate neuroinflammation and are implicated in a variety of neuropathologies. The present study investigated the antineuroinflammatory and neuroprotective effects of Gyejibokryeong-hwan (GBH), a traditional Korean medicine, in lipopolysaccharide- (LPS-) stimulated murine BV2 microglia. BV2 cells were pretreated with GBH, fluoxetine (FXT), or amitriptyline (AMT) for 1 h and then stimulated with LPS (100 ng/mL). The expression levels of nitric oxide (NO), cytokines, and chemokines were determined by the Griess method, ELISA, or real-time PCR. Western blotting was used to measure various transcription factors and mitogen activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K)/Akt activity. GBH significantly reduced the levels of NO, inducible nitric oxide synthase (iNOS), cyclooxygenase- (COX-) 2, tumor necrosis factor- (TNF-) α, interleukin- (IL-) 1β, IL-6, macrophage inhibitory protein- (MIP-) 1α, macrophage chemoattractant protein- (MCP-) 1, and IFN-γ inducible protein- (IP-) 10, regulated upon activation normal T cell expressed sequence (RANTES) in a dose-dependent manner. Expression of nuclear factor- (NF-) κB p65 was significantly decreased and phosphorylation of extracellular signal-regulated kinase (Erk), c-Jun NH2-terminal kinase (JNK), and PI3K/Akt by GBH, but not p38 MAPK, was decreased. Furthermore, production of anti-inflammatory cytokine IL-10 was increased and Heme oxygenase-1 (HO-1) was upregulated via the nuclear factor-E2-related factor 2 (NRF2)/cAMP response element-binding protein (CREB) pathway, collectively indicating the neuroprotective effects of GBH. We concluded that GBH may suppress neuroinflammatory responses by inhibiting NF-κB activation and upregulating the neuroprotective factor, HO-1. These results suggest that GBH has potential as anti-inflammatory and neuroprotective agents against microglia-mediated neuroinflammatory disorders.

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Antioxidant and Anti-Inflammatory Effects of Rhei Rhizoma and Coptidis Rhizoma Mixture on Reflux Esophagitis in Rats

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/2052180 ◽

2016 ◽

Vol 2016 ◽

pp. 1-13 ◽

Cited By ~ 17

Author(s):

O Jun Kwon ◽

Min Yeong Kim ◽

Sung Ho Shin ◽

Ah Reum Lee ◽

Joo Young Lee ◽

...

Keyword(s):

Reflux Esophagitis ◽

Heme Oxygenase 1 ◽

Esophageal Mucosa ◽

Dependent Manner ◽

Rhei Rhizoma ◽

Coptidis Rhizoma ◽

Anti Inflammatory ◽

And Control ◽

Dose Dependent

The purpose of this study was to investigate the antioxidant and anti-inflammatory effects of the combined extract of Rhei rhizoma and Coptidis rhizoma (RC-mix) in experimental model of acute reflux esophagitis. The antioxidant activity was assessed byin vitro2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. RC-mix was given at 100, 200, and 400 mg/kg body weight 2 h prior to induction of reflux esophagitis (RE). After 5 h, the effects of RC-mix treated rats were compared with those of normal and control rats. The representative flavonoid contents of RC-mix, such as sennoside A, epiberberine, coptisine, palmatine, and berberine, were detected using HPLC. The elevated esophageal mucosa damage was markedly ameliorated by RC-mix treatment in a dose-dependent manner. Furthermore, the administration of RC-mix reduced the increase of serum reactive oxygen species (ROS) and peroxynitrite (ONOO−). The improvement of superoxide dismutase (SOD) and heme oxygenase-1 (HO-1) levels were marked in the group given RC-mix. Moreover, the elevation of inflammatory mediators and cytokines by nuclear factor-kappa B (NF-κB) activation in control rats decreased by RC-mix pretreatment. These results indicate that RC-mix treatment reduces the pathological states of esophagitisviaregulating NF-κB mediated inflammation related to oxidative stress.

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Anti-Inflammatory and Anti-Oxidative Effects of luteolin-7-O-glucuronide in LPS-Stimulated Murine Macrophages through TAK1 Inhibition and Nrf2 Activation

International Journal of Molecular Sciences ◽

10.3390/ijms21062007 ◽

2020 ◽

Vol 21 (6) ◽

pp. 2007 ◽

Cited By ~ 1

Author(s):

Young-Chang Cho ◽

Jiyoung Park ◽

Sayeon Cho

Keyword(s):

Heme Oxygenase 1 ◽

No Production ◽

Related Factor ◽

Dependent Manner ◽

Nuclear Factor ◽

Quinone Oxidoreductase ◽

Murine Macrophages ◽

Nrf2 Activation ◽

Anti Inflammatory ◽

Oxidative Effects

Various herbal extracts containing luteolin-7-O-glucuronide (L7Gn) have been traditionally used to treat inflammatory diseases. However, systemic studies aimed at elucidating the anti-inflammatory and anti-oxidative mechanisms of L7Gn in macrophages are insufficient. Herein, the anti-inflammatory and anti-oxidative effects of L7Gn and their underlying mechanisms of action in macrophages were explored. L7Gn inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages by transcriptional regulation of inducible NO synthase (iNOS) in a dose-dependent manner. The mRNA expression of inflammatory mediators, including cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α), was inhibited by L7Gn treatment. This suppression was mediated through transforming growth factor beta-activated kinase 1 (TAK1) inhibition that leads to reduced activation of nuclear factor-κB (NF-κB), p38, and c-Jun N-terminal kinase (JNK). L7Gn also enhanced the radical scavenging effect and increased the expression of anti-oxidative regulators, including heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), and NAD(P)H quinone oxidoreductase 1 (NQO1), by nuclear factor-erythroid 2 p45-related factor 2 (Nrf2) activation. These results indicate that L7Gn exhibits anti-inflammatory and anti-oxidative properties in LPS-stimulated murine macrophages, suggesting that L7Gn may be a suitable candidate to treat severe inflammation and oxidative stress.

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Anti-Inflammatory Effect of Dohongsamultang through Inhibition of Nuclear Factor-κB Activation in Peripheral Blood Mononuclear Cells of Patients with Cerebral Infarction

The American Journal of Chinese Medicine ◽

10.1142/s0192415x0700493x ◽

2007 ◽

Vol 35 (03) ◽

pp. 415-426 ◽

Cited By ~ 2

Author(s):

Su-Jin Kim ◽

Hyun-Ja Jeong ◽

Sei-Uk Park ◽

Byung-Soon Moon ◽

Phil-Dong Moon ◽

...

Keyword(s):

Cerebral Infarction ◽

Mononuclear Cells ◽

Dependent Manner ◽

Nuclear Factor ◽

Peripheral Blood Mononuclear ◽

Inflammatory Reactions ◽

Tnf Α ◽

Anti Inflammatory ◽

Dose Dependent ◽

Inflammatory Effect

The Korean indigenous medicine "Dohongsamultang (DHSMT)" has long been used for various cerebrovascular diseases. However, the exact mechanism for the anti-inflammatory effect of DHSMT is not completely understood. The aim of the present study is to elucidate how DHSMT modulates the inflammatory reaction in lipopolysaccaride (LPS)-stimulated peripheral mononuclear cells from cerebral infarction (CI) patients. Production and expression of cytokine was measured via the ELISA and RT-PCR methods. The level of nuclear factor-kappa B (NF-κB)/Rel A protein and NF-κB DNA binding activity were determined via the Western blot analysis and transcription factor enzyme-linked immunoassay. It showed that DHSMT inhibited the production of TNF-α, IL-1β, and IL-6 induced by LPS in a dose-dependent manner ( p < 0.05). The maximal inhibition rates for TNF-α, IL-1β, and IL-6 production by DHSMT were about 50.18%, 32.13%, and 38.03%, respectively. DHSMT inhibited the TNF-α mRNA expression in a dose-dependent manner. We also showed that the inhibitory effect of DHSMT is through the suppression of the NF-κB pathway. The study suggests an important molecular mechanism by GMGHT to reduce inflammation, which might explain its beneficial effect in the regulation of inflammatory reactions.

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Sargachromenol Purified from Sargassum horneri Inhibits Inflammatory Responses via Activation of Nrf2/HO-1 Signaling in LPS-Stimulated Macrophages

Marine Drugs ◽

10.3390/md19090497 ◽

2021 ◽

Vol 19 (9) ◽

pp. 497

Author(s):

Eui-Jeong Han ◽

Thilina U. Jayawardena ◽

Jae-Hyuk Jang ◽

Ilekuttige Priyan Shanura Fernando ◽

Youngheun Jee ◽

...

Keyword(s):

Inflammatory Responses ◽

Mapk Signaling ◽

Sargassum Horneri ◽

Heme Oxygenase 1 ◽

Raw 264.7 ◽

Related Factor ◽

Nuclear Factor ◽

Raw 264.7 Macrophages ◽

Anti Inflammatory ◽

Inflammatory Effect

In this study, we isolated sargachromenol (SC) from Sargassum horneri and evaluated its anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. SC did not show cytotoxicity at all concentrations and effectively increased the cell viability by reducing the nitric oxide (NO) and intracellular reactive oxygen species (ROS) production in LPS-stimulated RAW 264.7 macrophages. In addition, SC decreased the mRNA expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α) and inflammatory mediators (iNOS and COX-2). Moreover, SC suppressed the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB) and mitogen-activated protein kinase (MAPK) signaling, whereas activated the nuclear factor erythroid 2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) signaling in LPS-stimulated RAW 264.7 macrophages. Interestingly, the anti-inflammatory effect of SC was abolished by the inhibition of HO-1 in LPS-stimulated RAW 264.7 macrophages. According to the results, this study suggests that the antioxidant capacity of SC leads to its anti-inflammatory effect and it potentially may be utilized in the nutraceutical and pharmaceutical sectors.

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Amomum tsao-ko Suppresses Lipopolysaccharide-Induced Inflammatory Responses in RAW264.7 Macrophages via Nrf2-Dependent Heme Oxygenase-1 Expression

The American Journal of Chinese Medicine ◽

10.1142/s0192415x14500773 ◽

2014 ◽

Vol 42 (05) ◽

pp. 1229-1244 ◽

Cited By ~ 24

Author(s):

Bin Li ◽

Hee-Jin Choi ◽

Dong-Sung Lee ◽

Hyuncheol Oh ◽

Youn-Chul Kim ◽

...

Keyword(s):

Heme Oxygenase ◽

Nuclear Translocation ◽

Inflammatory Responses ◽

Ethanol Extract ◽

Heme Oxygenase 1 ◽

Related Factor ◽

Nuclear Factor ◽

Neuroprotective Effects ◽

Raw264.7 Macrophages ◽

Anti Inflammatory

Amomum tsao-ko Crevost et Lemaire, used as a spice in Asia, is an important source of Chinese cuisine and traditional Chinese medicines. A. tsao-ko is reported to exert a variety of biological and pharmacological activities, including anti-proliferative, anti-oxidative and neuroprotective effects. In this study, NNMBS227, consisting of the ethanol extract of A. tsao-ko, exhibited potent anti-inflammatory activities in RAW264.7 macrophages. We investigated the effect of NNMBS227 in the suppression of pro-inflammatory mediators, including pro-inflammatory enzymes (inducible nitric oxide synthase and cyclooxygenase-2) and cytokines (tumor necrosis factor-α and interleukin-1β) in LPS stimulated macrophages. NNMBS227 also inhibited the phosphorylation and degradation of IκB-α, as well as the nuclear translocation of nuclear factor kappa B (NF-κB) p65 caused by stimulation with LPS. In addition, NNMBS227 induced heme oxygenase (HO)-1 expression through the nuclear translocation of nuclear factor E2-related factor 2 (Nrf2) in macrophages. Using tin protoporphyrin (SnPP), an HO activity inhibitor, we confirmed an association between the anti-inflammatory effects of NNMBS227 and the up-regulation of HO-1. These findings suggest that Nrf2-dependent increases in the expression of HO-1 induced by NNMBS227 conferred anti-inflammatory activities in LPS stimulated RAW264.7 macrophages.

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Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic Extract in LPS-Stimulated RAW 264.7 Macrophages

Mediators of Inflammation ◽

10.1155/2020/3164239 ◽

2020 ◽

Vol 2020 ◽

pp. 1-14

Author(s):

Yea-Jin Park ◽

Se-Yun Cheon ◽

Dong-Sung Lee ◽

Divina C. Cominguez ◽

Zhiyun Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Methanolic Extract ◽

Raw 264.7 Cells ◽

Heme Oxygenase 1 ◽

Prostaglandin E ◽

Raw 264.7 ◽

Related Factor ◽

Dependent Manner ◽

Nuclear Factor ◽

Anti Inflammatory

A hypernomic reaction or an abnormal inflammatory process could cause a series of diseases, such as cardiovascular disease, neurodegeneration, and cancer. Additionally, oxidative stress has been identified to induce severe tissue injury and inflammation. Carpesium cernuum L. (C. cernuum) is a Chinese folk medicine used for its anti-inflammatory, analgesic, and detoxifying properties. However, the underlying molecular mechanism of C. cernuum in inflammatory and oxidative stress conditions remains largely unknown. The aim of this study was to examine the effects of a methanolic extract of C. cernuum (CLME) on lipopolysaccharide- (LPS-) induced RAW 264.7 mouse macrophages and a sepsis mouse model. The data presented in this study indicated that CLME inhibited LPS-induced production of proinflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) in RAW 264.7 cells. CLME treatment also reduced reactive oxygen species (ROS) generation and enhanced the expression of heme oxygenase-1 (HO-1) protein in a dose-dependent manner in the LPS-stimulated RAW 264.7 cells. Moreover, CLME treatment abolished the nuclear translocation of nuclear factor-κB (NF-κB), enhanced the activation of nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), and reduced the expression of extracellular signal-related kinase (ERK) and ERK kinase (MEK) phosphorylation in LPS-stimulated RAW 264.7 cells. These outcomes implied that CLME could be a potential antioxidant and anti-inflammatory agent.

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Cannabisin F from Hemp (Cannabis sativa) Seed Suppresses Lipopolysaccharide-Induced Inflammatory Responses in BV2 Microglia as SIRT1 Modulator

International Journal of Molecular Sciences ◽

10.3390/ijms20030507 ◽

2019 ◽

Vol 20 (3) ◽

pp. 507 ◽

Cited By ~ 10

Author(s):

Shanshan Wang ◽

Qian Luo ◽

Peihong Fan

Keyword(s):

Oxidative Stress ◽

Inflammatory Response ◽

Signaling Pathway ◽

Heme Oxygenase 1 ◽

Mrna Levels ◽

Dependent Manner ◽

Nuclear Factor ◽

Bv2 Microglia ◽

Anti Inflammatory ◽

And Oxidative Stress

Hemp seed (Fructus cannabis) is rich in lignanamides, and initial biological screening tests showed their potential anti-inflammatory and anti-oxidative capacity. This study investigated the possible effects and underlying mechanism of cannabisin F, a hempseed lignanamide, against inflammatory response and oxidative stress in lipopolysaccharide (LPS)-stimulated BV2 microglia cells. Cannabisin F suppressed the production and the mRNA levels of pro-inflammatory mediators such as interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) in a concentration-dependent manner in LPS-stimulated BV2 microglia cell. Furthermore, cannabisin F enhanced SIRT1 expression and blocked LPS-induced NF-κB (Nuclear factor kappa B) signaling pathway activation by inhibiting phosphorylation of IκBα (Inhibit proteins of nuclear factor kappaB) and NF-κB p65. And the SIRT1 inhibitor EX527 significantly inhibited the effect of cannabisin F on pro-inflammatory cytokines production, suggesting that the anti-inflammatory effects of cannabisin F are SIRT1-dependent. In addition, cannabisin F reduced the production of cellular reactive oxygen species (ROS) and promoted the expression of Nrf2 (Nuclear factor erythroid-2 related factor 2) and HO-1 (Heme Oxygenase-1), suggesting that the anti-oxidative effects of cannabisin F are related to Nrf2 signaling pathway. Collectively, these results suggest that the neuro-protection effect of cannabisin F against LPS-induced inflammatory response and oxidative stress in BV2 microglia cells involves the SIRT1/NF-κB and Nrf2 pathway.

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