Clinical Significance of Tumor Necrosis Factor in Patients with Bronchogenic Carcinoma and Benign Lung Diseases: A Comparative Study

1992 ◽  
Vol 7 (2) ◽  
pp. 103-106 ◽  
Author(s):  
M. Rapellino ◽  
F. Pecchio ◽  
G. Aimo ◽  
G. Priolo ◽  
S. Baldi ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Pulmonary Fibrosis ◽  
Lung Carcinoma ◽  
Healthy Subjects ◽  
Lung Diseases ◽  
Tumor Necrosis ◽  
Advanced Disease ◽  
Pulmonary Diseases ◽  
Control Group ◽  
Necrosis Factor

Tumor Necrosis Factor (TNF) was determined in the serum of 72 lung carcinoma patients. Twenty-four healthy subjects younger than 50 years and 10 healthy subjects older than 70 years were considered as control group. TNF was also measured in 20 patients with stage I sarcoidosis and in 15 patients with pulmonary fibrosis. The marker was detected in 32% of cases in the neoplastic group, in 37.5% of disease confined to the chest and in 25% of advanced disease cases. A large proportion of TNF-positive samples was found in sarcoidosis (30%), and even larger in pulmonary fibrosis (66.6%). TNF was also present in healthy subjects older than 70 (40%). We conclude that TNF is not specific of malignancy, being demonstrable in other benign pulmonary diseases and even in the course of physiological aging.

Association between tumor necrosis factor-α gene-1031T/C promoter polymorphism and endometriosis in a European population

2019 ◽  
Vol 40 (2) ◽  
Author(s):  
Anastasia Drakou ◽  
Despoina Mavrogianni ◽  
Konstantinos Ntzeros ◽  
Athanasios Protopapas ◽  
Petros Drakakis ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Promoter Polymorphism ◽  
European Population ◽  
Pcr Analysis ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Healthy Women ◽  
Tnf Α ◽  
Necrosis Factor

Abstract Background Tumor necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine which plays an important role in the pathogenesis of many diseases. Endometriosis is one of the most common gynecological diseases. The purpose of this study was to investigate the association of TNF-α-1031T/C polymorphism with the genetic susceptibility of endometriosis in a European population. Materials and methods In this case-control study, 51 endometriosis patients and 67 healthy control women participated. We used endometrial tissue from the patients and peripheral blood from the healthy women to extract DNA. Polymerase chain reaction (PCR) analysis and the restriction enzyme Bbs I were used to analyze the -1031 T/C polymorphism in the TNF-α gene promoter region. Statistical analysis was performed using Fisher’s exact test. We also calculated the odds ratios. Results In the group of patients, 66.7% of women were detected with the TT genotype, 33.3% with the TC genotype and 0% with the CC genotype while in the control group, 46.3% had the TT genotype, 47.8% had the TC genotype and 6% had the CC genotype. There was a significant association between the TT genotype with endometriosis (p = 0.03). There was no significant deviation from the Hardy-Weinberg equilibrium. Conclusions The TC and CC genotypes appeared more often in the healthy women than the endometriosis patients and this shows that the C allele might have a protective role in endometriosis in the Greek population. Further studies are needed to specify the role of this polymorphism in pathogenesis of endometriosis and the mechanisms that protect the patients from the disease.

Importance of Markers of Sepsis in Surgical Patients

2018 ◽  
Vol 84 (6) ◽  
pp. 1058-1063 ◽  
Author(s):  
Marek Smolár ◽  
Ivana Dedinská ◽  
Michal Hošala ◽  
Július Mazúch ◽  
L'Udovit Laca
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Serum Levels ◽  
Control Group ◽  
Risk Of Death ◽  
Significant Difference ◽  
Important Position ◽  
Factor Α ◽  
Necrosis Factor ◽  
Septic Condition

Sepsis, severe sepsis, and septic shock represent a serious medicinal and general social problem and still maintain an important position among the present issues in the basic and clinical research. In the prospective analysis of patients satisfying the criteria of septic condition, we determined serum levels of bioparameters in three consecutive days from the first signs of sepsis depending on the stage or advancement of the septic condition. We determined the most significant parameter/parameters which are able to determine the stage of sepsis or to predict patient's death. In the group of 68 patients, all monitored biomarkers showed significant difference in serum concentrations versus the control group (P = 0.001). The strongest positive connection between the seriousness of sepsis and serum level is in case of procalcitonin. Predictor of mortality (r = -0.468; P = 0.001), transferrin (r = -0.506; P = 0.003), and tumor necrosis factor-α (r = 0.939; P = 0.001). Our results show that the monitored parameters (procalcitonin, C-reactive protein, tumor necrosis factor-a, and interleukin 6) have strong correlations between the serum levels and the stage of disease. Examination of at least one cytokine in normal clinical practice might lead to better interpretation of the patient's condition, determining the risk of death.

Effects of Prostacyclin on Hemodynamics after Intestinal Ischemia-Reperfusion

1997 ◽  
Vol 5 (1) ◽  
pp. 43-47
Author(s):  
S Fehmi Katircioğlu ◽  
Eser Özgencil ◽  
Birol Yamak ◽  
Tulga Ulus ◽  
Selime Ayaz ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Reperfusion Injury ◽  
Tumor Necrosis ◽  
Intestinal Ischemia ◽  
Ischemia Reperfusion ◽  
Venous Pressure ◽  
Control Group ◽  
Central Venous ◽  
Intestinal Ischemia Reperfusion ◽  
Necrosis Factor

Ten rabbits underwent 30 minutes of superior mesenteric artery occlusion to assess the release of tumor necrosis factor, subcellular damage, and hemodynamic changes after intestinal ischemia-reperfusion injury. Five were treated with prostacyclin 5 ng/kg/min 5 minutes before the arterial occlusion. It was increased to 25 ng/kg/min during occlusion, decreased to 5 ng/kg/min for the first 5 minutes of reperfusion, and then discontinued. A control group of 5 rabbits did not receive any pharmacological agent. Specimens were obtained from the small intestine for electron microscopy after 10 minutes and after 60 minutes of reperfusion, while simultaneous blood samples were collected for measurement of tumor necrosis factor. Minimal changes were seen in tissue from the prostacyclin group but severe mitochondrial damage and vacuolation occurred in the control group. The tumor necrosis factor level was 11.97 ± 3.17 U/mL in the control group and 5.06 ± 2.19 U/mL in the prostacyclin group, one hour after the end of mesenteric occlusion ( p < 0.05). Hemodynamic status, assessed by central venous and arterial pressures, was much more affected in the control group than in the prostacyclin group. Mean arterial pressure was 71 ± 5 mm Hg in the control group, and 91 ± 6 mm Hg in the prostacyclin group ( p < 0.05). Central venous pressure was 5.3 ± 0.9 mm Hg in the control group and 2.3 ± 0.7 mm Hg in the prostacyclin group ( p < 0.05). We conclude that intravenous prostacyclin reduced the severity of reperfusion injury occurring during the early period of reperfusion by inhibiting the release of the toxic mediator tumor necrosis factor, thus decreasing distant organ injury.

scholarly journals Circulating CTRP1 Levels Are Increased and Associated with the STOD in Essential Hypertension in Chinese Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Zhengjia Su ◽  
Shuya Tian ◽  
Wei Liang
Keyword(s):  
Essential Hypertension ◽  
Tumor Necrosis Factor ◽  
Healthy Subjects ◽  
Tumor Necrosis ◽  
Target Organ Damage ◽  
Organ Damage ◽  
Left Ventricular ◽  
Chinese Patients ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrosis Factor

This study aimed to investigate the correlation between complement C1q tumor necrosis factor-related protein 1 (CTRP1) and subclinical target organ damage (STOD) in essential hypertension (EH). 720 patients were enrolled in this study, including 360 healthy subjects and 360 patients with EH. The EH group included 183 patients complicated with STOD and 177 patients without STOD. In the STOD group, there were 87 patients with left ventricular hypertrophy (LVH), 32 patients with microalbuminuria (MAU), and 58 patients with complication of LVH and MAU. Enzyme-linked immunosorbent assay (ELISA) was used to detect the CTRP1, adiponectin (APN), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). We found that CTRP1 levels were higher in patients with EH than those in healthy subjects; moreover, the level of CTRP1 of patients in the group complicated with EH and STOD was increased compared with EH patients without STOD. CTRP1 levels in the group complicated with LVH and MAU were significantly higher than those in the LVH group and the MAU group. Furthermore, APN, CTRP1, and IL-6 were three factors that influenced the STOD of EH patients, among which CTRP1 and IL6 were positively related with the complication of hypertension and STOD. In conclusion, CTRP1 levels are increased and associated with the STOD (heart and kidney) in essential hypertension, which can be regarded as a novel biomarker in the prediction of prognosis for patients with essential hypertension.

The change of proinflammatory cytokine tumor necrosis factor α level in the use of meloxicam in rat model of osteoarthritis

2019 ◽  
Vol 30 (6) ◽  
Author(s):  
Junaidi Khotib ◽  
Naning Windi Utami ◽  
Maria Apriliani Gani ◽  
Chrismawan Ardianto
Keyword(s):  
Tumor Necrosis Factor ◽  
Rat Model ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Control Group ◽  
Treatment Groups ◽  
Tnf Α ◽  
Α Level ◽  
Factor Α ◽  
Necrosis Factor

Abstract Background Osteoarthritis (OA) is a chronic disease in the joints. One of the proinflammatory cytokines that is thought to have a major role in the inflammatory process, the emergence of pain, and cartilage damage in OA is tumor necrosis factor α (TNF-α). Meloxicam is a nonsteroidal anti-inflammatory drug class of drugs that is relatively selective in inhibiting the activity of cyclooxygenase 2 (COX-2) formation. This study is conducted to prove the change in TNF-α level in the use of meloxicam with model in animals suffering from OA. Methods The OA rat model was induced with sodium monoiodoacetate intra-articularly. Rats were divided into 5 groups: negative control group, positive control group, and treatment groups with various doses of meloxicam. Hyperalgesia effect was evaluated using a warm plate test, and TNF-α level was determined using enzyme-linked immunosorbent assay. Results The treatment groups that received meloxicam at a dose of 1.0, 3.0, or 10.0 mg/kg body weight (BW) did not show significant differences in rat knee joint diameter (p = 0.99), but showed a significant difference in sensitivity to heat stimulation (p = 0.02) compared to the control group. Osteoarthritis rats experienced a significant reduction in TNF-α level after being given meloxicam at a dose of 10 mg/kg BW compared with the control group. This shows that the 10 mg/kg BW of meloxicam is a potential dose in reducing the TNF-α level in OA rat models. Conclusions Based on these data, it can be concluded that the inhibition of pain and the development of OA by meloxicam in animal models may be assigned to a decreased level of TNF-α.

scholarly journals Association of C1q/TNF-related protein-1 (CTRP1) serum levels with coronary artery disease

2019 ◽  
Vol 47 (6) ◽  
pp. 2571-2579 ◽  
Author(s):  
Linhui Shen ◽  
Shuhong Wang ◽  
Yuan Ling ◽  
Wei Liang
Keyword(s):  
Coronary Artery Disease ◽  
Coronary Artery ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Multiple Logistic Regression Analysis ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Artery Disease ◽  
Necrosis Factor

Objective Complement C1q tumor necrosis factor-related proteins (CTRPs), belonging to the CTRP superfamily, are extensively involved in regulating metabolism and the immune-inflammatory response. The inflammatory process is linked to the pathogenesis of coronary artery disease (CAD). Here, we investigated the association of serum levels of CTRP1 with CAD. Methods Study participants were divided into two groups according to the results of coronary angiography: a control group (n = 63) and a CAD group (n = 76). The concentrations of serum CTRP1 and inflammatory cytokines were determined by enzyme-linked immunosorbent assay. Further analysis of CTRP1 levels in individuals with different severities of CAD was conducted. The CAD severity was assessed by Gensini score. Results Serum levels of CTRP1 were significantly higher in CAD patients than in controls (17.24 ± 1.07 versus 9.31 ± 0.56 ng/mL), and CTRP1 levels increased with increasing severity of CAD. CTRP1 levels were positively correlated with concentrations of tumor necrosis factor-α and interleukin-6. Multiple logistic regression analysis showed that CTRP1 was significantly associated with CAD. Conclusions Our data showed close associations of serum CTRP1 levels with the prevalence and severity of CAD, indicating that CTRP1 can be regarded as a novel and valuable biomarker for CAD.

scholarly journals PSIX-9 Dietary β-sitosterol improves immunity and antioxidant status in broilers

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 346-346
Author(s):  
Yefei Cheng ◽  
Yueping Chen ◽  
Chao Wen ◽  
Yanmin Zhou
Keyword(s):  
Superoxide Dismutase ◽  
Tumor Necrosis Factor ◽  
Immunoglobulin G ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Control Group ◽  
Hepatic Glutathione ◽  
Factor Α ◽  
Necrosis Factor ◽  
Serum Tumor Necrosis

Abstract The application effects of phytosterols on poultry production have been reported. However, due to the mixed compositions, it is difficult to explain their action mode and physiological functions. β-sitosterol, the most abundant phytosterol, is naturally widespread in plant products. Information, however, is scarce in terms of effects of β-sitosterol on broilers. This research therefore investigated effects of dietary β-sitosterol with different levels on immunity and antioxidant capacity in broilers. Two hundred and forty one-day-old chicks were randomly allocated into five treatments of six replicates. Chickens in the five groups were fed a basal diet supplemented with 0 (control group), 40, 60, 80, and 100 mg/kg β-sitesterol for 42 days, respectively. Data were analyzed by one-way analysis of variance. Polynomial contrasts were used to test the linear and quadratic response to β-sitosterol levels. Difference among groups was evaluated using Tukey’s test, which was considered significant if P &lt; 0.05. Dietary β-sitosterol linearly increased (P &lt; 0.05) contents of serum immunoglobulin G and hepatic glutathione, activities of serum superoxide dismutase and catalase, whereas linearly decreased (P &lt; 0.05) concentrations of serum tumor necrosis factor α and hepatic malondialdehyde. Serum interleukin 1β level was linearly and quadratically reduced by β-sitosterol inclusion (P &lt; 0.05). Compared with the control group, dietary β-sitosterol with higher levels than 60 mg/kg increased concentrations of serum immunoglobulin G and hepatic glutathione, activities of serum superoxide dismutase and catalase (P &lt; 0.05), whereas decreased serum tumor necrosis factor α content (P &lt; 0.05). Its dosages higher than 40 mg/kg reduced serum interleukin 1β content (P &lt; 0.05), and 100 mg/kg of which lowered hepatic malondialdehyde concentration (P &lt; 0.05). The results indicated that dietary β-sitosterol could improve immunity and antioxidant ability in broilers. Also, a level of 80 mg/kg β-sitosterol supplementation was recommended into broiler diet.

scholarly journals Tumor Necrosis Factor-Alpha in Peripical Tissue Exudates of Teeth with Apical Periodontitis

2007 ◽  
Vol 2007 ◽  
pp. 1-4 ◽  
Author(s):  
Sonja Pezelj-Ribaric ◽  
Karolina Magašic ◽  
Jelena Prpic ◽  
Ivana Miletic ◽  
Zoran Karlovic
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Control Group ◽  
Radiological Findings ◽  
Necrosis Factor Alpha ◽  
Root Canals ◽  
Factor Alpha ◽  
Radiological Changes ◽  
Necrosis Factor

Aim.The aim of this study was to determine tumor necrosis factor-alpha (TNF-α) levels in periapical exudates and to evaluate their relationship with radiological findings.Methodology.Periapical exudates were collected from root canals of 60 single-rooted teeth using absorbent paper points. TNF-αlevels were determined by enzyme-linked immunosorbent assays. The samples were divided into three groups according to the periapical radiolucent area.Results.Nonparametric Kruskal-Wallis test revealed significant differences between TNF-αconcentrations in control group (40,57±28, 15 pg/mL) and group with larger radiolucent areas (2365,79±582, 95 pg/mL), as well as between control and canals with small radiolucent areas (507,66±278, 97) (P<.05).Conclusions.The levels of TNF-αincrease significantly in teeth with periapical pathosis, from smaller to bigger lesions. This research and its results have shown that objective analysis of the TNF-αlevels enables establishment of a relationship between different concentrations of TNF-αand different radiological changes.

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