scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Lasiocephalus longipenicillatus (Senecio longipenicillatus)

2006 ◽  
Vol 1 (2) ◽  
pp. 1934578X0600100 ◽  
Author(s):  
María Rondón ◽  
María Araque ◽  
Antonio Morales ◽  
María Gualtieri ◽  
Janne Rojas ◽  
...  

The analysis of the essential oil of Lasiocephalus longipenicillatus by GC-MS, yielded α-pinene (48.3%), α-humulene (15.8%) and germacrene-D (15.5%) as major constituents. The essential oil showed a strong antibacterial activity against important human pathogenic bacteria, such as Staphylococcus aureus and Enterococcus faecalis.

2008 ◽  
Vol 3 (3) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Janne Rojas ◽  
Judith Velasco ◽  
Antonio Morales ◽  
Luis Rojasa ◽  
Tulia Díaz ◽  
...  

The essential oil from the leaves of Baccharis trinervis (Lam.) Pers., collected in May 2006, was analyzed by GC/MS. A yield of 0.2% oil was obtained by hydrodistillation. Forty three components were identified by comparison of their mass spectra with those in the Wiley GC-MS Library data base. The major components were germacrene D (20.4%), limonene (15.4%), δ-cadinene (5.2%), β-caryophyllene (4.8%), α-pinene (4.5%) and bicyclogermacrene (4.0%). The essential oil showed antibacterial activity against the important human pathogenic Gram positive bacteria Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212, with MIC values of 80 and 200 μg/mL, respectively.


2010 ◽  
Vol 5 (2) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
José Moreno ◽  
Rosa Aparicio ◽  
Judith Velasco ◽  
Luis B Rojas ◽  
Alfredo Usubillaga ◽  
...  

The hydrodistilled oil from the fruits of Bursera tomentosa, obtained in 0.2% yield, was analyzed by GC-MS. Nine components were identified, which made up 99.3% of the oil. The most abundant constituents were cis-ocimene (47.6%), n-nonane (28.2%) and germacrene-D (11.1%). The oil showed antibacterial activity against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212) and Salmonella typhi (CDC 57), with MIC values of 80 μg/mL, 120 μg/mL and 100 μg/mL, respectively.


2008 ◽  
Vol 3 (6) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Teresa Díaz ◽  
Flor D. Mora ◽  
Judith Velasco ◽  
Tulia Díaz ◽  
Luis B. Rojas ◽  
...  

The chemical constituents of the essential oil obtained by hydrodistillation from the leaves of Calycolpus moritzianus (O. Berg) Burret, syn Psidium caudatum Mc Vaught, collected in November 2006 in Mérida State, Venezuela, were identified by GC-MS analysis. Thirty components (91.1% of the sample) were identified, of which the seven major ones were β-caryophyllene (21.9%), α-pinene (10.9%), viridiflorol (9.7%), β-selinene (6.1%), α-copaene (6.3%), α-selinene (5.3%) and γ-eudesmol (5.1%). The oil was found to have antibacterial activity against Staphylococcus aureus ATCC (6538) and Enterococcus faecalis ATCC (29212), with MIC values of 60 μg/mL and 180 μg/mL, respectively.


2007 ◽  
Vol 2 (12) ◽  
pp. 1934578X0700201 ◽  
Author(s):  
Janne Rojas ◽  
Judith Velasco ◽  
Luis B. Rojas ◽  
Tulia Díaz ◽  
Juan Carmona ◽  
...  

The essential oils from leaves of Baccharis latifolia and B. prunifolia collected in January 2006 were analyzed by GC/MS. The yields of oils extracted by hydrodistillation were 0.27 and 0.29% for B. latifolia and B. prunifolia, respectively. Sixteen (B. latifolia) and twenty nine (B. prunifolia) components were identified by comparison of their mass spectra with the Wiley GC-MS Library data and by their retention indices (RI). The identified products may be divided into four different groups: monoterpenes (9.0% B. latifolia; 43.9% B. prunifolia), oxygenated monoterpenes (0.8% B. latifolia; 5.4% B. prunifolia), sesquiterpenes (20.4% B. latifolia; 45.9% B. prunifolia) and oxygenated sesquiterpenes (69.8% B. latifolia; 1.9% B. prunifolia). The oils showed antibacterial activity only against Gram positive bacteria, with MIC values for Staphylococcus aureus (ATCC 25923) of 80 μg/mL (B. latifolia) and Enterococcus faecalis (ATCC 29212) of 90 μg/mL and 260 μg/mL (B latifolia and B. prunifolia, respectively).


2020 ◽  
Vol 10 (2-s) ◽  
pp. 7-13
Author(s):  
Habiba BOUKHEBTI ◽  
I. Demirtas ◽  
L. Omar ◽  
A.N. Chaker

The aim of this study is to identify the chemical composition and to evaluate the antimicrobial activity of Chrysanthemum morifolium. The analysis and identification of essential oil which obtained by hydrodistilation method were realized by gaz chromatography and mass spectroscopy. The antibacterial activity was tested by using the agar diffusion test and the Gram positive and negative pathogenic bacteria: Staphylococcus aureus ATCC 25923. Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Citrobacter freundii ATCC 8090, Kleibseilla pneumoniae ATCC 700603 and Shigella sonnei were used to evaluate this activity. This analysis led to the identification of 26 compounds representing 88.40 % of the total essential oil mass. The major compound was Verbenone (17.33 %). Other components present in appreciable contents were: Chrysanthenone (9.71%). 4-epi-cubedol (07.25%) and δ-Cadinol (05.29 %). Essential oil of Chrysanthemum morifolium exhibited an antibacterial effect against pathogenic bacteria, like those observed against Staphylococcus aureus ATCC 25923 (35±1.2mm) and Citrobacter freundii ATCC 8090 (21±0.87mm), however Pseudomonas aeruginosa  ATCC  27853  and  Kleibseilla  pneumoniae  ATCC  700603                      were resistant. The anatomical study showed the presence of several types of trichomes including the glands secreted for essential oils and protector trichomes. Keywords: essential oil, antibacterial activity, Chrysanthemum morifolium, anatomical study, chemical composition


Bionatura ◽  
2019 ◽  
Vol 4 (3) ◽  
pp. 908-912
Author(s):  
Jesús E. Velásquez ◽  
Luis B. Rojas-Fermín ◽  
Judith Velasco ◽  
Rosa L. Aparicio ◽  
Alfredo N. Usubillaga ◽  
...  

This work presents a comparative study of the chemical composition of the essential oils obtained from two varieties of Centrolobium paraense (orinocense EO o and paraense EO p) from Venezuela. GC analyzed the oils, and the constituents were identified by GC-MS and retention indices. Eighteen compounds were identified in EO o, which made up 88.9 % of the oil, but only sixteen compounds were identified in EO p, which made up 95.5 % of the total oil. β-caryophyllene (35.1 %) and -humulene (35.3 %) are the main constituents of EO p. The main constituents of EO o are α-humulene (24.8 %), β-caryophyllene (14.3 %), caryophyllene oxide (18.33 %), and humulene epoxide II (16.86 %). The biological activity of both oils was assayed. They were found to be equally active against Staphylococcus aureus and Enterococcus faecalis, with MIC of 100 and 600 μL/mL respectively. This is the first report describing the chemical composition of the essential oil of these species and their antibacterial activity.


2015 ◽  
Vol 10 (7) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Flor D. Mora ◽  
Yesenia L. Rojas ◽  
Viviana González ◽  
Judith Velasco ◽  
Tulia Díaz ◽  
...  

The composition and antimicrobial activity of the essential oil from Verbesina negrensis Steyerm. leaves is reported. Analysis was performed by GC/MS. Major constituents were α-pinene (43.1%), α-humulene (13.8%), δ-cadinene (8.1%), limonene (4.6%) and bicyclogermacrene (4.2%). The essential oil showed in vitro activity against the Gram-positive bacteria Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212 (MIC 500 and 350 μL/mL respectively).


2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.


2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Alexis Peña ◽  
Luis Rojas ◽  
Rosa Aparicio ◽  
Libia Alarcón ◽  
José Gregorio Baptista ◽  
...  

The essential oil of the leaves of Espeletia nana Cuatrec, obtained by hydrodistillation, was analyzed by GC-MS, which allowed the identification of 24 components, which made up 99.9% of the oil. The most abundant compounds were α-pinene (38.1%), β-pinene (17.2%), myrcene (15.0%), spathulenol (4.2%), bicyclogermacrene (4.0%), α-zingiberene (4.0%), and γhimachalene (3.7%). Antibacterial activity was tested against Gram-positive and Gram-negative bacteria using the agar disk diffusion method. Activity was observed only against Gram-positive bacteria. MIC values were determined for Staphylococcus aureus ATCC 25923(200 μg/mL) and Enterococcus faecalis ATCC 29212 (600 μg/mL).


2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095325
Author(s):  
Bala Namata Abba ◽  
Abderrahmane Romane ◽  
Amadou Tidjani Ilagouma

Endostemon tereticaulis (poir.) M.Ashby is a species of the Lamiaceae family present in Niger. This plant is used in traditional medicine due to its various biological potentialities. The present study investigated the chemical composition of the essential oil and the antibacterial activity of the essential oil and ethanolic extract of Endostemon tereticaulis against resistant pathogenic bacteria. Gas chromatography-mass spectrometry analysis of the essential oil led to the identification of 43 compounds representing 99.55% of the total essential oil. The major components were caryophyllene oxide (15.17%) followed by α-humulene (13.96%), α-copaene (11.75%), ( E)-β-caryophyllene (8.44%), and δ-cadinene (6.78%). The antibacterial activity was tested against multiresistant Acinetobacter baumannii P1483, Salmonella spp. H1548, extended-spectrum β-lactamase- Escherichia coli Bu8566, Enterobacter cloacae Bu147, Proteus mirabilis Bu190 , Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922), Enterococcus faecium H3434, methicillin-resistant Staphylococcus aureus P1123, and Staphylococcus aureus (ATCC 25923). The antibacterial assays revealed that the essential oil was more active than the ethanolic extract against the studied bacteria with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values ranging from 0.06 to 2 mg/mL. Also, the ethanolic extract was effective against the bacteria tested with MIC and MBC values ranging from 0.12 to 3 mg/mL. This study showed that Endostemon tereticaulis essential oil is rich in bioactive compounds. Ethanolic extract and essential oil exhibited potential antibacterial activity. These results provide a scientific basis for the use of this plant in traditional medicine. The current study described for the first time the antibacterial activity of Endostemon tereticaulis.


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