scholarly journals In Vitro Anti-inflammatory and Xanthine Oxidase Inhibitory Activity of Tephrosia purpurea Shoot Extract

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Shivraj H. Nile ◽  
Chandrahasy N. Khobragade
Keyword(s):  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Cow Milk ◽  
Diene Conjugate ◽  
Tephrosia Purpurea ◽  
Oxidase Enzyme ◽  
Anti Inflammatory

The methanolic extract of Tephrosia purpurea (Leguminosae) shoots was evaluated in-vitro for its anti-inflammatory and xanthine oxidase inhibitory activity. Anti-inflammatory activity was measured by the Diene-conjugate, HET-CAM and β-glucuronidase methods. The enzyme inhibitory activity was tested against isolated cow milk xanthine oxidase. The average anti-inflammatory activity of T. purpurea shoot extract in the concentration range of 1-2 μg/mL in the reacting system revealed significant anti-inflammatory activities, which, as recorded by the Diene-conjugate, HET-CAM and β-glucuronidase assay methods, were 45.4, 10.5, and 70.5%, respectively. Screening of the xanthine oxidase inhibitory activity of the extract in terms of kinetic parameters revealed a mixed type of inhibition, wherein the Km and Vmax values in the presence of 25 to 100 μg/mL shoot extract was 0.20 mM/mL and 0.035, 0.026, 0.023 and 0.020 μg/min, while, for the positive control, the Km and Vmax values were 0.21 mM/mL and 0.043 μg/min, respectively. These findings suggest that T. purpurea shoot extract may possess constituents with good medicinal properties that could be exploited to treat the diseases associated with oxidative stress, xanthine oxidase enzyme activity and inflammation.

scholarly journals HPLC Analysis, Antioxidant, Anti-inflammatory and Xanthine Oxidase Inhibitory Activity of Cudrania tricuspidata

2015 ◽  
Vol 10 (11) ◽  
pp. 1934578X1501001
Author(s):  
Shivraj Hariram Nile ◽  
Doo Hwan Kim
Keyword(s):  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
Folk Medicine ◽  
Positive Control ◽  
Significant Activity ◽  
Diene Conjugate ◽  
Antioxidant Power ◽  
Cudrania Tricuspidata ◽  
Free Radical Formation ◽  
Anti Inflammatory

Cudrania tricuspidata is a plant used in folk medicine in Korea for treatment of diseases related to oxidative stress and inflammation. In this study the leaf and shoot extract was studied for its antioxidant, anti-inflammatory, and xanthine oxidase (XO) inhibitory activities. The extract with predominant phenolics was quantified using HPLC-DAD. Antioxidant activity was measured using ABTS [2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) and FRAP (ferric ion reducing antioxidant power), and anti-inflammatory activity by diene-conjugate and β-glucuronidase assays. The average antioxidant effects of C. tricuspidata extracts (1–2 mg/mL) revealed significant activity of 32.5% to 50.2% (ABTS) and 24.2 to 40.5% (FRAP) compared with Trolox, having 55.1% (ABTS) and 42.6% (FRAP) activity, respectively. The anti-inflammatory activities showed as 26.5% to 40.5% (DC) and 40.2% to 70.2% (βG) inhibition compared with the control {phenylbutazone; 42.1% (DC) and 80.2% (βG) inhibition, respectively}. The XO inhibitory activity of the plant extract revealed 90.5% inhibition of that of the control (allopurinol) (97% inhibition at 100 μg/mL concentration). The kinetic parameters of XO inhibition revealed a noncompetitive type of inhibition, where, K m and V max of C. tricuspidata extracts (25 to 100 μg/mL) were 0.25 mM/mL and 0.040, 0.036, 0.032, and 0.030 (μg/min), while for the positive control K m and V max the values were 0.30 mM/mL and 0.045 (μg/min), respectively. Results suggest that C. tricuspidata can be exploited against diseases associated with free radical formation and xanthine oxidase activity.

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF TINOCRISPOSIDE BY INHIBITING NITRIC OXIDE PRODUCTION IN LIPOPOLYSACCHARIDES-STIMULATED RAW 264.7 CELLS

2018 ◽  
Vol 11 (4) ◽  
pp. 149 ◽  
Author(s):  
Adek Zamrud Adnan ◽  
Muhammad Taher ◽  
Tika Afriani ◽  
Annisa Fauzana ◽  
Dewi Imelda Roesma ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inflammatory Diseases ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
No Production ◽  
Raw 264.7 ◽  
Dependent Manner ◽  
Anti Inflammatory

 Objective: The aim of this study was to investigate in vitro anti-inflammatory activity of tinocrisposide using lipopolysaccharides (LPS)-stimulated RAW 264.7 macrophage cells. Tinocrisposide is a furano diterpene glycoside that was isolated in our previous study from Tinospora crispa.Methods: Anti-inflammatory effect was quantified spectrometrically using Griess method by measuring nitric oxide (NO) production after the addition of Griess reagent.Results: The sample concentrations of 1, 5, 25, 50, and 100 μM and 100 μM of dexamethasone (positive control) have been tested against the LPS-stimulated RAW 264.7 cells, and the results showed NO level production of 39.23, 34.00, 28.9, 20.25, 16.3, and 13.68 μM, respectively, and the inhibition level of 22.67, 33.00, 43.03, 60.10, 68.00, and 73%, respectively.Conclusions: From the study, it could be concluded that tinocrisposide was able to inhibit the formation of NO in the LPS-stimulated RAW 264.7 cells in concentration activity-dependent manner, with half-maximal inhibition concentration 46.92 μM. It can be developed as anti-inflammatory candidate drug because NO is a reactive nitrogen species which is produced by NO synthase. The production of NO has been established as a mediator in inflammatory diseases.

scholarly journals Phytochemical screening and in vitro anti-inflammatory activity of ethanolic extract of Epidendrum coryophorum leaves.

Bionatura ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 1387-1393
Author(s):  
Irina Francesca González Mera ◽  
Orestes Darío López Hernández ◽  
Vivian Morera Córdova
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Anti Inflammatory

Epidendrum coryophorum belongs to the Orchidaceae family. Traditional uses of some species for this genus include infusions of the leaves used for kidney problems, treat influenza, conjunctivitis, liver pain, relieve kidney symptoms, and hypoglycemic effect. This work's objective was to determine the phytochemical profile of the ethanolic extract of Epidendrum coryophorum leaves and to evaluate the potential anti-inflammatory activity in vitro of the extract employing the erythrocyte membrane stabilization method. The phytochemical screening carried out in this work suggested phenols, coumarins, flavonoids, tannins, steroids, and sterols in the ethanolic extract of Epidendrum coryophorum leaves. Cardiotonic glycosides and carbohydrates were also found. The ethanolic extract's UV-Vis spectrum showed absorption maxima at 268 nm and 332 nm, which could correspond to flavonoids of the flavonoid classes, 3-OH substituted flavonols, or isoflavones. The quantitative determination of total phenols of the ethanolic extract was carried out using the Folin-Ciocalteu method. The total phenolic content expressed as mg Gallic acid equivalent (G.A.E.) per gram of extract was found to be 19,96 mgGAE/g of Epidendrum coryophorum. The ethanolic extract of Epidendrum coryophorum leaves showed hemolysis inhibition values ​​of 18,19% at 1,0 mg/mL, 38,98% at 1,5 mg/mL and 40,94% at 2,5 mg/mL compared with aspirin (positive control) giving values ​​of 65,33% at 1,0 mg/mL, 72,26% at 1,5 mg/mL and 73,75% at 2,5 mg/mL. The values ​​obtained for inhibition of hemolysis with ethanolic extract, compared with the values ​​obtained with a pure anti-inflammatory, are significant and demonstrate anti-inflammatory activity in Epidendrum coryophorum. Keywords: Epidendrum coryophorum, total phenolic content, microencapsulation, anti-inflammatory activity

scholarly journals IN VITRO AND IN SILICO EVALUATION OF XANTHINE OXIDASE INHIBITORY ACTIVITY OF QUERCETIN CONTAINED IN SONCHUS ARVENSIS LEAF EXTRACT

2017 ◽  
Vol 10 (14) ◽  
pp. 50
Author(s):  
Rini Hendriani ◽  
Nursamsiar Nursamsiar ◽  
Ami Tjitraresmi
Keyword(s):  
Free Energy ◽  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
In Silico ◽  
Leaf Extract ◽  
Binding Free Energy ◽  
Uv Spectrophotometry ◽  
Docking Simulations ◽  
Oxidase Enzyme

Objective: The aim of the present study was to examine the inhibiting effects of quercetin contained in Sonchusarvensis leaf extract on the activity of xanthine oxidase, an essential enzyme for uric acid synthesis.Methods: Activity test was conducted in vitro by measuring the activity of xanthine oxidase using UV spectrophotometry and in silico by determining the interaction of quercetin and allopurinol (as comparation drug) with xanthine oxidase enzyme in terms of hydrogen bonds and binding free energy. Docking simulations were performed by Autodock4.2 package.Results: The active fraction, using the solvent n-hexane, ethyl acetate and water, tested the inhibitory activity of the xanthine oxidase enzyme in vitro obtained respectively IC50 of 263.19, 16.20 and 141.80 μg/ml. Isolates with highest activity identified as quercetin. The xanthine oxidase enzyme inhibitory activity insilico by molecular docking showed quercetin has free energy binding ˗7.71 kcal/mol, more negative than that of allopurinol ˗5.63 kcal/mol.Conclusion: This shows the affinity of quercetin stronger than that of allopurinol; so that it can be predicted that quercetin was more potential to inhibit xanthine oxidase enzyme activity. Thus the extract of the S. arvensis leaves containing the active compound quercetin was a potential use as antihyperuricemia.  

scholarly journals Synthesis of some New Azo Compounds of Salicylic Acid Derivatives and Determination of their In vitro Anti-inflammatory Activity

2019 ◽  
Vol 35 (2) ◽  
pp. 870-876
Author(s):  
Husam Hamza Salman ◽  
Huda Salih Abood ◽  
Usama Hamid Ramadhan
Keyword(s):  
Salicylic Acid ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Azo Compounds ◽  
Significant Activity ◽  
Diazo Coupling ◽  
Salicylic Acid Derivatives ◽  
Stability Method ◽  
Anti Inflammatory

This study included the preparation of a series of some new azo compounds by diazo coupling aromatic amines with salicylic acid derivatives. The prepared compounds identified using precise elemental analysis (C.H.N.), the results supported the structure of concerned compounds. The synthesized azo compounds also identified by using infrared spectroscopy and 1H-NMR spectroscopy. Anti-inflammatory activity of the compounds were determined in-vitro by human red blood cell (HRBC) membrane stability method, the compounds showed a significant activity to protection of the cell membrane. Other compounds show moderate to low activity, sodium diclofenac was used as positive control.

scholarly journals IN VITRO ANTI-INFLAMMATORY ACTIVITY OF 4-BENZYLPIPERIDINE

2016 ◽  
pp. 108 ◽  
Author(s):  
Jayashree V ◽  
Bagyalakshmi S ◽  
Manjula Devi K ◽  
Richard Daniel D
Keyword(s):  
Inhibitory Activity ◽  
Protein Denaturation ◽  
In Vitro Models ◽  
Inflammatory Activity ◽  
Significant Activity ◽  
Standard Drug ◽  
Study Results ◽  
Anti Inflammatory ◽  
Dose Dependent

<p>ABSTRACT<br />Objective: To study the in vitro anti-inflammatory activity of 4-benzylpiperidine.<br />Methods: This study was conducted to evaluate the in vitro anti-inflammatory activity of 4-benzylpiperidine using in vitro models such as inhibition<br />of albumin denaturation and proteinase inhibitory activity.<br />Results: This study revealed the dose-dependent inhibition of protein denaturation and proteinase inhibitory activity by 4-benzylpiperidine.<br />Conclusion: In the present study, results indicate that the 4-benzylpiperidine possess anti-inflammatory properties. The drug inhibited the heat<br />induced albumin denaturation and proteinase inhibitory activity. It shows dose-dependent significant activity when compared with a standard drug.<br />Hence, this study gives an idea that the 4-benzylpiperidine can be used as a lead compound for designing a potent anti-inflammatory drug which can<br />be used to cure inflammation.<br />Keywords: Anti-inflammatory activity, 4-Benzylpiperidine, Protein denaturation, Proteinase inhibitory activity.</p>

scholarly journals The anti-arthritic and anti-inflammatory activities of aqueous extract powder bark of Anthocleista schweinfurthii Gilg (Loganiaceae)

2018 ◽  
Vol 8 (6-s) ◽  
pp. 174-181
Author(s):  
Valdi Steve Djova ◽  
Ascension Maximilienne Nyegue ◽  
François-Xavier Etoa
Keyword(s):  
Aqueous Extract ◽  
Selective Inhibition ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Inflammatory Effect

  Aqueous extract bark of Anthocleista schweinfurthii (ASE) was investigated for anti-arthritic, anti-inflammatory activities and phytochemicals screening. Phytochemical screening was carried out according, to LC-MS procedures, the anti-inflammatory effect of ASE was evaluated in vitro inhibition of 15-lipoxygenase (15-LOX) enzyme and  proteinase inhibiting action assays. The anti-arthritic activity was performed according to the Bovine Serum Albumin (BSA) test. The chemical composition of ASE showed that it was mainly composed of isocyanate, biphenyltriol, methoxyflavanone, soscoparin and glucopyroside. In the anti-inflammatory assays, ASE showed selective inhibition of 15-lipooxygenase with IC50 value of and moderate activity against the inhibition of proteinase action with IC50 value of L. Both the methods  showed that the ASE posses good anti-inflammatory activity. ASE had strong in vitro anti-arthritic activity with IC50 of , the positive control Diclofenac had IC50 value of  The result of this study justified the use of this plant in traditional Cameroonian medicine in the treatment of inflammation. ASE may offer a new source of potential therapeutic agents for the effective treatment of arthritis and inflammation.    Keywords: Anthocleista schweinfurthii, phytochemical screening, anti-arthritic activity, anti-inflammatory activity,     

scholarly journals Formulation and Evaluation of Poly Herbal Liqui-Solid Compact for its Anti-Inflammatory Effect

2021 ◽  
Vol 12 (3) ◽  
pp. 3883-3899
Keyword(s):  
Inhibitory Activity ◽  
Hibiscus Cannabinus ◽  
Inflammatory Activity ◽  
Scanning Calorimetry ◽  
Disintegration Time ◽  
Test Species ◽  
Powder Formulation ◽  
Anti Inflammatory

Liquisolid approach is a potential method due to the ease in the production process, low cost, and manufacturing process because of their strong flow and compaction properties. The study's main goal is to create a liquisolid powder formulation from the polyherbal extract and evaluate its anti-inflammatory activity. Hibiscus cannabinus, Murraya koenigii, and Tabernaemontana divaricate are used to formulating polyherbal Liquisolid powder formulation to assess their anti-inflammatory action. The formulation is tested for its stability studies and anti-inflammatory activity, both in-vitro and in-vivo. The polyherbal liquisolid powder formulation (F5) has a minimum strength of 1kg/cm 2, while F2 & F6 strength was 4 kg/cm2; FTIR studies showed no interactions between drug and excipients. To confirm the liquisolid powder formulation, further evaluations on friability, hardness, disintegration time, dissolution rate, and Differential scanning calorimetry and X-ray powder diffraction analysis were determined. The polyherbal extract's anti-inflammatory activities were evaluated using the paw edema and cotton pellet method, and the results are found to exhibit its inhibitory activity. The polyherbal extract of Liquisolid powder formulation was evaluated, and it showed a promising inhibitory activity to overcome the inflammation in the test species. Hence it can be used as an alternative therapy for curing inflammation.

scholarly journals Colchicine Induced Mutation in Nigella sativa Plant for the Assessment of Morpho-Physiological and Biochemical Parameter Vis-A-Vis In Vitro Anti-Inflammatory Activity

2021 ◽  
Vol 15 (1) ◽  
pp. 173-182
Author(s):  
Garima Gupta ◽  
Anjuman Gul Memon ◽  
Brijesh Pandey ◽  
Mohd Sajid Khan ◽  
Mohammed Shariq Iqbal ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Nigella Sativa ◽  
Colchicine Treatment ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
P Value ◽  
Induced Mutation ◽  
Anti Inflammatory ◽  
Physiological And Biochemical

Background: Nigella sativa (NS), an herbaceous medicinal plant recognized for its diverse beneficial applications as a spice and traditional medicine. Objective: The present study was targeted to explore the antioxidant potential of Nigella sativa in response to colchicine-induced mutation. The stress condition brought due to mutation may affect the medicinal value (anti-inflammatory activity) of the plant. Method: Nigella sativa seeds were imperiled to colchicine treatment at various concentrations viz. 0.00625, 0.0125, 0.025, 0.05 and 0.1% subjected for analysis. Result: The colchicine treated plant (polyploid/ mutant) at 0.025% concentrations showed significant variation at morpho-physiological and biochemical level with respect to control (p value < 0.05). At the morphological level, the plant showed enlargement of shoot length (33.760±2.295mm), root length (13.546±1.535 mm), and leaf area (22.836±1.524 mm2). The analysis of seeds showed enhanced seeds per pod (49.333±4.163), weight of seeds (2.810±0.010g), length (3.133±0.089mm), and width (1.123±0.044mm) when compared with control. The physiological parameters also showed significant enhancement for stomatal index (35.456±4.751%), chlorophyll A (9.053±0.865 µg/gfw), chlorophyll B (4.990±0.763 µg/gfw), and total carotene content (773.190±5.906 µg/gfw). However, the fresh weight/ dry weight ratio (10.738±3.031) was found to be deprived. Furthermore, biochemical parameters viz. total flavonoid (seeds 1.973±0.134; plant 1.703 ± 0.064 mg eqv QE/g of tissue), total phenolic (seeds 15.690±1.495; plant 8.220±0.070 mg eqv GA/g of tissue), total carotene (seeds nil; plant 773.190±5.906 µg/gfw), and total antioxidant (seeds 0.445±0.102; plant 0.386±0.010 mM eqv AA/g tissue) were significantly elevated at 0.025% of colchicine treatment. When the in vitro anti-inflammatory activity was targeted, a significant escalation was observed for inhibition of albumin denaturation (97.466±2.835%), proteinase inhibitory activity (62.290±6.475%), heat-induced hemolysis (89.873±3.533%), hypotonicity induced hemolysis (92.572±3.527%), anti-lipoxygenase activity (96.010±3.098%), and cyclooxygenase inhibitory activity (68.296±3.920%) at 500µg/mL concentration of extract. Conclusion: Thus, it can be concluded that 0.025% of colchicine can induce significant (p value < 0.05) mutation in the Nigella sativa plant, which may lead to alterations at morpho-physiological and biochemical levels. Such treatment induces stress in the plant and leads to elevated antioxidant levels. This in turn elevates the therapeutic potential of the plant. Hence, our study is a novel and open-ended finding to explore various other medical properties of the plant with respect to colchicine-induced mutation.

The Anti-Inflammatory Activity of Pentacyclic Triterpenes In Vitro

2011 ◽  
Vol 138-139 ◽  
pp. 1174-1178
Author(s):  
Li Ming Bai ◽  
Fan Xu Meng ◽  
Li Jie Zhao ◽  
Ming Zhao
Keyword(s):  
Ursolic Acid ◽  
Inhibitory Activity ◽  
Inflammatory Activity ◽  
Nerium Oleander ◽  
Significant Activity ◽  
Pentacyclic Triterpenes ◽  
Anti Inflammatory

The Anti-inflammatory activity of pentacyclic triterpenes, oleananes (1,2), urasanes (3-11), lupanes (12,13), taraxasteranes (14,15), which isolated from Nerium oleander L, were examined. Ursolic acid (5) showed significant activity and compounds (1, 12) and (14) showed weak to modelate activity toward inhibitory activity on ICAM-1 expression.

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