scholarly journals Two New Biisoflavonoids from the Roots of Daphne Oleoides

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701
Author(s):  
Shazia Yasmeen ◽  
Muhammad Aijaz Anwar ◽  
Sadia Ferheen ◽  
Nighat Afza ◽  
Abdul Malik ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Soluble Fraction ◽  
2D Nmr ◽  
Soluble Extract ◽  
Isolation And Identification ◽  
Phytochemical Investigation

Phytochemical investigation of the ethyl acetate soluble fraction of the methanol soluble extract of the roots of Daphne oleoides resulted in isolation and identification of two new isomeric biisoflavonoids characterized as 8,8′’-bi-6-hydroxyorobol (1) and 8,8′’-bi-6, 2′-dihydroxygenistein (2). The structures of these compounds were established by analysis of their 1D and 2D NMR and HRMS data.

scholarly journals CONTRIBUTE TO STUDY ON CHEMICAL CONSTITUENTS OF URENA LOBATA (L.) OF VIETNAM

2019 ◽  
Vol 57 (2) ◽  
pp. 162
Author(s):  
Quan Minh Pham ◽  
Hoai Van Thi Tran ◽  
Lam Tien Do ◽  
Phuong Lan Doan ◽  
Inh Thi Cam ◽  
...  
Keyword(s):  
Mental Disorders ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
2D Nmr ◽  
Spectroscopic Methods ◽  
Tree Roots ◽  
Chemical Structures ◽  
Phytochemical Investigation ◽  
First Time

Urena lobata L. is used in Vietnamese traditional medicine for the treatment of several diseases. Tree roots are used to treat rheumatism, dysentery, poor digestion, flu, tonsils, malaria, asthma, goiter. Flowers are used to treat chickenpox, fever, and mental disorders. Branches, leaves or whole trees used to treat injuries bruises, rheumatism, mastitis, bites. Phytochemical investigation of the n-hexan and ethyl acetate extract of Urena lobata L. led to the isolation of β-sitosterol (1), β-sitosterol-3-O-β-D-glucopyranoside (2), a-acetylamino-phenylpropyl a-benzoylamino-phenylpropanoate (3), quercetin (4), and trans-tiliroside (5). Their chemical structures were determined by spectroscopic methods including MS, 1D, 2D NMR and comparing with those reported in previous papers. Two compounds 3, 5 were isolated for the first time from Urena lobata plant.

scholarly journals Chemical Constituents of Cichorium intybus and their Inhibitory Effects against Urease and α-Chymotrypsin Enzymes

2011 ◽  
Vol 6 (8) ◽  
pp. 1934578X1100600
Author(s):  
Sumayya Saied ◽  
Shazia Shah ◽  
Zulfiqar Ali ◽  
Ajmal Khan ◽  
Bishnu P. Marasini ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
2D Nmr ◽  
Cichorium Intybus ◽  
Spectroscopic Techniques ◽  
Inhibitory Effects ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Hydroxyphenylacetic Acid ◽  
First Time

Phytochemical investigation of the aerial parts of Cichorium intybus L. resulted in the isolation and identification of two new natural metabolites, 2,6-di[but-3( E)-en-2-onyl]naphthalene (1), and 3,3′,4,4′-tetrahydroxychalcone (2), along with nine known compounds. Their structures were determined by spectroscopic techniques including 1D and 2D NMR. The known compounds were identified as scopoletin (3), 4-hydroxyphenylacetic acid (4), 3-hydroxy-4-methoxybenzoic acid (5), 4,4′-dihydroxychalcone (6), 6,7-dihydroxycoumarine (7), 1-triacontanol (8), lupeol (9), β-sitosterol (10), and β-sitosterol-3- O-β-glucopyranoside (11). Compounds 4-6 and 8 are reported for the first time from C. intybus. Compounds 2 and 3 showed weak inhibitory activities against urease and α-chymotrypsin enzymes, respectively.

scholarly journals Phenolic Compounds from Centaurea horrida L Growing in Lebanon

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Rima Boukhary ◽  
Maha Aboul-ElA ◽  
Othman Al-Hanbali ◽  
Abdalla El-Lakany
Keyword(s):  
Phenolic Compounds ◽  
Middle East ◽  
Ethyl Acetate ◽  
Caffeic Acid ◽  
Folk Medicine ◽  
Isolation And Identification ◽  
Phytochemical Investigation ◽  
First Time

Centaurea horrida has been used in folk medicine for many purposes as diuretic, antiinflammatory, hypotensive, antidiarrhetic, mild astringent, bitter tonic, stomachic, digestive, and emmenagogue and in cosmetics. An infusion of this plant is used to treat diabetes and digestive problems Also it is used as a wash for tired eyes and in conjunctivitis. C.horrida grown in Lebanon is widely used in the treatment of diarrhea, as antihypertensive and as hypoglycemic. Phytochemical investigation of the species grown in Lebanon led to the isolation and identification of five phenolic compounds isolated from methanol, ethyl acetate and butanol fractions of Centaurea extracts. Their structures were determined by NMR, MS, UV and IR methods. It is important to mention that the isolated flavonoids fisetin, hispidulin, quercetin, quercetin -3-D- galactoside and caffeic acid were isolated for the first time from Centaurea horrida. L growing in the Middle East.

Chemical Constituents of Adesmia Boronioides and Evaluation of their Antioxidant Activity

2015 ◽  
Vol 8 (3) ◽  
pp. 2919-2223
Author(s):  
Orlando Muñoz ◽  
C Delporte
Keyword(s):  
Chemical Constituents ◽  
Antioxidant Properties ◽  
2D Nmr ◽  
Isolation And Identification ◽  
Ongoing Research ◽  
Phytochemical Investigation ◽  
Kaempferol Glycoside ◽  
Pharmacologically Active ◽  
Pharmacologically Active Compounds ◽  
First Time

As part of our ongoing research into the pharmacologically active compounds of Chilean flora, here we did phytochemical investigation of Adesmia boronioides Hook.f (fabaceae). (Papilonaceae) leaves. This effort has led to isolation and identification of liqueritigenin, isoliquiritigenin, sesamin, isodrimenin and a kaempferol glycoside, kaempferol 3-O-β-D-[galactopyranosyl  (1–2) rhamnoside]-7-O –α-L-rhamnopyranoside.Compound structures were identified by 1D- and 2D-NMR experiments, mass and UV spectrometry and comparison with data from the literature. This is the first time these compounds have been isolated from this plant’s leaves.These active constituents have been studied further pharmacologically. Antioxidant properties in A. boronioides leaves were determined.

scholarly journals Activity-guided Isolation and Identification of Antioxidant Components from Ethanolic Extract of Peltiphyllum Peltatum (Torr.) Engl

2008 ◽  
Vol 3 (8) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Solomon Habtemariam
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
2D Nmr ◽  
Light Petroleum ◽  
Spectroscopic Methods ◽  
Isolation And Identification ◽  
Water Fractions ◽  
Radical Damage

Activity-directed fractionation and isolation procedures were used to identify the antioxidant components of Peltiphyllum peltatum. Fresh leaves were extracted with ethanol and subsequently fractionated into light petroleum, chloroform, ethyl acetate, n-butanol, and water fractions. The ethyl acetate fraction, which showed the strongest antioxidant activity, was subjected to Sephadex LH-20 chromatography, followed by repetitive preparative TLC, to afford the major antioxidant principle, quercitrin, together with other antioxidants (isoquercitrin, catechin and gallate) as minor principles. The identities of the isolated compounds were established by spectroscopic methods (1D and 2D NMR and MS) and by direct comparison with authentic samples and literature values. Quercitrin displayed a potent protective effect of DNA from UV generated hydroxyl radical damage.

Isolation and identification of further peptides in the diafiltration retentate of the water-soluble fraction of Cheddar cheese

1997 ◽  
Vol 64 (3) ◽  
pp. 433-443 ◽  
Author(s):  
TANOJ K. SINGH ◽  
PATRICK F. FOX ◽  
ÁINE HEALY
Keyword(s):  
Soluble Fraction ◽  
Cell Envelope ◽  
Cheddar Cheese ◽  
Water Soluble ◽  
Soluble Extract ◽  
Amino Acid Sequencing ◽  
Isolation And Identification ◽  
Water Soluble Fraction ◽  
Water Soluble Extract ◽  
Hydrolysis Of

Several peptides were isolated from the diafiltration retentate, prepared using 10 kDa membranes, of the water-soluble extract from a commercial mature Cheddar cheese and identified by amino acid sequencing and mass spectrometry. Most of the peptides were from the N-terminal half of β-casein, but peptides from αs1- and αs2-caseins were also identified; the extract also contained α-lactalbumin. Identified peptides showed the important role played by lactococcal cell envelope proteinases in the degradation of primary proteolytic products from αs1- and β- caseins, produced by chymosin and plasmin respectively. Plasmin seemed to be involved in the hydrolysis of αs2-casein. Several phosphopeptides were identified and the action of phosphatase on these peptides was evident.

scholarly journals Phytochemical, Antimicrobial and Antiplasmodial Investigations of Terminalia brownii

2013 ◽  
Vol 8 (6) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Francis Machumi ◽  
Jacob O. Midiwo ◽  
Melissa R. Jacob ◽  
Shabana I. Khan ◽  
Babu L. Tekwani ◽  
...  
Keyword(s):  
Ellagic Acid ◽  
Soluble Fraction ◽  
2D Nmr ◽  
Stem Bark ◽  
Bark Extract ◽  
Spectroscopic Methods ◽  
Group 4 ◽  
Phytochemical Investigation ◽  
Stem Bark Extract ◽  
Ellagic Acid Derivatives

Phytochemical investigation of the ethyl acetate-soluble fraction of stem bark extract of an African medicinal plant Terminalia brownii led to the isolation of a new oleanane-type triterpenoid, along with seven known triterpenoids, seven ellagic acid derivatives, and 3- O-β-D-glucopyranosyl-β-sitosterol. The new compound was identified using spectroscopic methods, notably 1D- and 2D NMR, as 3β,24- O-ethylidenyl-2α,19α-dihydroxyolean-12-en-28-oic acid. The isolated compounds were evaluated for their antimicrobial and antiplasmodial activities. Two compounds with a galloyl group (4 and 6) were found to be active against chloroquine sensitive (D6) and chloroquine resistant (W2) strains of Plasmodium falciparum, whereas three ellagic acid derivatives (5–7) were found active against three species of fungi and one species of bacteria.

scholarly journals PHYTOCHEMICAL INVESTIGATION OF CHENOPODIUM MURALE (FAMILY: CHENOPODIACEAE) CULTIVATED IN IRAQ, ISOLATION AND IDENTIFICATION OF SCOPOLETIN AND GALLIC ACID

2017 ◽  
Vol 10 (11) ◽  
pp. 70
Author(s):  
Omer H Ahmed ◽  
Maha N Hamad ◽  
Noor S Jaafar
Keyword(s):  
Ethyl Acetate ◽  
Gallic Acid ◽  
Phenolic Acid ◽  
High Performance ◽  
Chemical Constituents ◽  
Ethyl Acetate Fraction ◽  
Absolute Methanol ◽  
Isolation And Identification ◽  
Chenopodium Murale ◽  
Phytochemical Investigation

  Objective: The aim of our study was to investigate chemical constituents of leaves of Chenopodium murale since no phytochemical investigation had been done previously in Iraq.Methods: Leaves of C. murale were macerated in absolute methanol for 2 days and fractionated by petroleum ether, chloroform, ethyl acetate, and n-butanol. The ethyl acetate fraction was analyzed by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) for its phenolic acid and coumarins contents. Coumarin derivative and phenolic acid were isolated from this fraction and identified by gas chromatography/mass spectrometry, infrared, ultraviolet, HPLC, and HPTLC.Results: The different chromatographic and spectroscopic results revealed the presence of gallic acid and coumarin.Conclusion: The results of the current study showed the presence of scopoletin and gallic acid in the ethyl acetate fraction of C. murale.

scholarly journals Activity-guided Isolation and Identification of Free Radical-scavenging Components from Ethanolic Extract of Boneset (Leaves of Eupatorium Perfoliatum)

2008 ◽  
Vol 3 (8) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Solomon Habtemariam
Keyword(s):  
Ethyl Acetate ◽  
Protocatechuic Acid ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
2D Nmr ◽  
Isolation And Identification ◽  
Similar Treatment ◽  
Eupatorium Perfoliatum

Activity-directed fractionation and isolation procedures were used to identify the antioxidant components of Boneset (Eupatorium perfoliatum). Dried powdered leaves were extracted with ethanol and subsequently fractionated into light petroleum, chloroform, ethyl acetate, and n-butanol fractions. The ethyl acetate fraction, which showed the strongest antioxidant activity, reducing power and highest phenolic content, was subjected to Sephadex LH-20 chromatography followed by repetitive preparative TLC to afford protocatechuic acid as the major antioxidant constituent, together with small amounts of other antioxidants (hyperoside, quercetin and rutin). Similar treatment of the n-butanol fraction, the second most potent fraction, yielded predominantly rutin and a trace amount of hyperoside as active constituents. The identity of the compounds was established based on direct comparison of spectral data (UV, IR, 1D- and 2D-NMR and MS) with those of authentic samples and literature values.

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