scholarly journals 6-Methoxyflavonol Glycosides with In Vitro Hepatoprotective Activity from Chenopodium Bonus-henricus Roots

2015 ◽  
Vol 10 (8) ◽  
pp. 1934578X1501000
Author(s):  
Zlatina Kokanova-Nedialkova ◽  
Magdalena Kondeva-Burdina ◽  
Dimitrina Zheleva-Dimitrova ◽  
Virginia Tzankova ◽  
Stefan Nikolov ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Linoleic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Rat Hepatocytes ◽  
2D Nmr ◽  
Cellular Damage ◽  
Flavonoid Glycosides ◽  
Frap Assay

One new, namely 6-methoxykaempferol 3- O-[β-apiofuranosyl(1→2)]-β-glucopyranosyl(1→6)-β-glucopyranoside (2), and two known flavonoid glycosides, spinacetin 3- O-[β-apiofuranosyl(1→2)]-β-glucopyranosyl(1→6)-β-glucopyranoside (1) and spinacetin 3- O-gentiobioside (3), were isolated from the roots of Chenopodium bonus-henricus L. Their structures were determined by means of spectroscopic methods (1D, 2D NMR, UV, IR) and HR-ESI-MS. Radical scavenging and anti-oxidant activities of 1 and 3 were established using DPPH and ABTS free radicals, FRAP assay and inhibition of lipid peroxidation (LP) in a linoleic acid system by the ferric thiocyanate method. Compound 3 was found to possess stronger DPPH and ABTS radical scavenging activity (IC50 0.44 ± 0.008 mM and 0.089 ± 0.002 mM, respectively) compared with 1 (IC50 1.22 ± 0.010 mM and 0.11 ± 0.004 mM, respectively). Both flavonoids inhibited the lipid peroxidation of linoleic acid significantly. Additionally, 1 and 3 significantly reduced the cellular damage caused by the hepatotoxic agent CCl4 in rat hepatocytes and preserved cell viability and GSH level, decreased LDH leakage and reduced lipid damage. Effects were similar to those of the positive control silymarin. Control of self-toxic effects made in a MTT based assay using HepG2 cells revealed statistically significant cytotoxic effects only in very high concentrations (exceeding mM) and an incubation time of 72 h, making flavonoid glycosides with a 6-methoxykaempferol skeleton a promising and safe class of hepatoprotective compounds.

scholarly journals In-vitro Antioxidant and Anti-lipid Peroxidation Activity of Ethanolic Extracts of Bougainvillea shubhra, Bougainvillea peruviana and Bougainvillea bhuttiana Golden Glow: A Comparative Study

2015 ◽  
Vol 15 (1) ◽  
pp. 43 ◽  
Author(s):  
Mosmi Medpilwar ◽  
Darshil Maru ◽  
Meenakshi Upadhyay ◽  
Neha Lavania ◽  
Madhavi Vernekar ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Frap Assay ◽  
Ethanolic Extracts ◽  
In Vitro Inhibition ◽  
Reducing Power Assay

The aim of our present study was to evaluate the antioxidant and anti-lipid peroxidation activity of ethanolic extracts of <em>Bougainvillea species viz B. shubhra, B. peruviana and B. bhuttiana</em> golden glow. Phytochemical constituents viz. total phenolics, flavonoids and tannins were assayed using standard protocol. The antioxidant activity was studied by DPPH assay, FRAP assay, reducing power assay and in-vitro inhibition of lipid peroxidation. Of the three species, <em>B. bhuttiana</em> golden glow showed highest amount of phenols (6.78±0.001), flavonoids (27.7±0.012) and tannin (11.08±0.008) contents. It also showed highest antioxidant activity of 15.15± 0.008 and 8.08± 0.018 as determined by FRAP assay and reducing power assay respectively. The percent DPPH radical scavenging activity was 82.72% and in-vitro inhibition of lipid peroxidation in mitochondrial membrane was also found to be highest in <em>B. bhuttiana</em> golden glow.

scholarly journals IMPROVEMENT OF ORGANIC RED PIGMENT PRODCUTION BY MONASCUS PURPUREUS TISTR3651 USING PATHUMTHANI-1 RICE-BASED MEDIUM IN SUBMERGED AND SOLID-STATE FERMENTATION

2021 ◽  
pp. 47-50
Author(s):  
SURACHAI TECHAOEI ◽  
KHEMJIRA JARMKOM ◽  
THISAKORN DUMRONGPHUTTIDACHA ◽  
WARACHATE KHOBJAI
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Monascus Purpureus ◽  
Total Phenolic ◽  
Red Pigment ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Monascus Pigments ◽  
Ferric Reducing Antioxidant Power

Objective: This research is to study the production of natural red pigment by Monascus purpureus TISTR3615 in the submerged and solid-statefermentation system using Pathumthani-1 rice as a carbon source.Methods: The antioxidant activity of the red pigment was evaluated in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH), ABTS radical scavenging assay,and ferric-reducing antioxidant power (FRAP) assay, including total phenolic compound.Results and Discussion: The maximum of red pigment production was 0.55±0.02/ml (OD 680 nm) after incubation at 30°C for 24 days. Theantioxidant activity based on inhibition DPPH (%), ABTS radical scavenging activity (%), and FRAP activity (mM Fe2+/g) was 97.80±1.51,68.64±0.46, and 0.32±0.021, respectively. The total phenolic content was 164.78±2.82 μg GAE/mg.Conclusion: It was estimated that Monascus pigments, leading to nutraceutical and pharmaceutical applications, cosmetic industry, and foodindustry.

scholarly journals Chemical Composition, Antibacterial and Antioxidant Activity of the Essential Oil of Bupleurum longiradiatum

2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Baojun Shi ◽  
Wei Liu ◽  
Shao-peng Wei ◽  
Wen-jun Wu
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gas Chromatography Mass Spectrometry ◽  
Dpph Radical ◽  
Bacterial Strains ◽  
Bupleurum Longiradiatum

The essential oil from the roots of Bupleurum longiradiatum, obtained by hydrodistillation was analyzed by gas chromatography/mass spectrometry (GC/MS) and evaluated for antimicrobial activity and antioxidant activity. Fifty-one compounds were identified, representing 99.3% of the total oil. The major constituents were thymol (7.0%), butylidene phthalide (6.8%), 5-indolol (5.6%), heptanal (5.3%), 4-hydroxy-2-methylacetophenone (5.3%), 4,5-diethyl-octane (5.3%), bormeol (5.1%) and hexanoic acid (5.1%). The oil was tested against 4 bacteria at different concentrations using disc diffusion and 96-well dilution methods. The inhibition zones and minimum inhibitory concentration values for bacterial strains were in the range of 7.0–18.0 mm and 250 −500 μg/mL, respectively. The in vitro antioxidant activity was assessed by DPPH radical scavenging and inhibition of lipid peroxidation methods. The oil showed a potent free radical scavenging activity, as evidenced by the low IC50 value for DPPH radical (566.2μg/mL) and inhibition of lipid peroxidation (induced by FeSO4, H2O2 and CCl4) with IC50 values of 275.2 μg/mL, 296.9 μg/mL and 118.7 μg/mL, respectively.

scholarly journals In vitro Bioactivity of Essential Oils and Methanol Extracts of Salvia reuterana from Iran

2012 ◽  
Vol 7 (5) ◽  
pp. 1934578X1200700
Author(s):  
Javad Safaei Ghomi ◽  
Reihaneh Masoomi ◽  
Fereshteh Jookar Kashi ◽  
Hossein Batooli
Keyword(s):  
Linoleic Acid ◽  
Essential Oils ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Methanol Extracts ◽  
Phenolic Contents ◽  
Β Carotene

The chemical composition of the essential oils, antioxidant activity (DPPH and β-carotene/linoleic acid assays) and total phenolic content (Foline-Ciocalteu) of the flowers and leaves of Salvia reuterana were determined. Essential oils extracted from the flowers and leaves by hydrodistillation were analyzed by GC and GC/MS. Forty-four constituents, representing 99.7-99.9% of the oils, were identified. The major components were germacrene D, benzoic acid hexyl ester, bicyclogermacrene, β-gurjunene and ishwarene, constituting 33.7-31.9% of the oils. The highest radical-scavenging activity (DPPH test) was shown by the methanol extract of the flowers (IC50=77.6 μg/mL). In the β-carotene/linoleic acid assay, the methanol extract of the leaves showed the highest inhibition (40.3%) which was only slightly lower than that shown by BHT (82.9%). The total phenolic contents of the methanol extracts of the flowers and leaves as gallic acid equivalents were 81.4 and 88.3 μg/mg, respectively. The plant also showed good antimicrobial activity against three strains of tested microorganisms.

scholarly journals Antioxidant Activity and Bioactive Compounds of Lamium album Flower Extracts Obtained by Supercritical Fluid Extraction

2021 ◽  
Vol 11 (16) ◽  
pp. 7419
Author(s):  
Pascaline Aimee Uwineza ◽  
Anna Gramza-Michałowska ◽  
Marcin Bryła ◽  
Agnieszka Waśkiewicz
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Array Detector ◽  
Frap Assay ◽  
Constant Flow Rate ◽  
Lamium Album

In this research, supercritical CO2 extraction is applied to extract bioactive compounds from Lamium album (white dead nettle, Lamiaceae). Extraction was performed at various temperatures (40, 50, and 60 °C) using methanol as co-solvent at a constant flow rate of CO2, methanol, and pressure. The collected extracts were characterized in terms of antioxidant capacity by using DPPH, ABTS and FRAP in vitro antioxidant activity assays, whereas the Folin–Ciocalteu procedure was employed to estimate the total phenols content (TPC). On the other hand, phenolic compounds in the extracts were quantitated by liquid chromatography coupled with a photodiode array detector (UPLC-PDA) and confirmed with a mass detector (TQD). The extracts have shown high TPC ranged between 234.17 to 650.17 mg GAE/g extract. DPPH scavenging of the extracts was estimated and obtained EC50 values ranged from 0.12 to 0.37 mg/mL of solution. The ABTS radical scavenging activity ranged from 43.20 to 44.53 µg TE/g. The FRAP value was found within the range of 19.48 to 44.74 µmol TE/g of extract. Differences between extraction conditions were observed. In this research, 50 °C/250 bar was efficient for the TPC, DPPH, ABTS, and FRAP assays; moreover, statistically, TPCs and FRAP assay showed significant differences between the conditions at α = 0.05. The identification of phenolic compounds in the obtained extract of Lamium album flowers, using UPLC/PDA, revealed that chrysin, pinostrobin, myricetin, and trans-3-hydroxycinnamic acid were the significant molecules present, which may be responsible for the high content of polyphenols and antioxidant activity. The results obtained indicated that SC-CO2 could be considered an alternative method for extracting bioactive compounds of Lamium album. High antioxidant activity and the presence of various bioactive compounds indicate the potential of this plant from the Lamiaceae family and the possibility of its application in various industries, including agriculture, food technology, or pharmacy.

Antioxidant Activities of Three Dihydrochalcone Glucosides from Leaves of Lithocarpus pachyphyllus

2004 ◽  
Vol 59 (7-8) ◽  
pp. 481-484 ◽  
Author(s):  
Wei-Min Yang ◽  
Ji-Kai Liu ◽  
Xiang-Dong Qin ◽  
Wan-Lin Wu ◽  
Zhi-He Chen
Keyword(s):  
Lipid Peroxidation ◽  
Inhibitory Concentration ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Effective Concentration ◽  
Scavenging Activity ◽  
Sod Activity

AbstractIn vitro antioxidant activities of three sweet dihydrochalcone glucosides from the leaves of Lithocarpus pachyphyllus (Kurz) Rehd. (Fagaceae), trilobatin 2″-acetate (1), phloridzin (2) and trilobatin (3), were investigated. The IC50 (50% inhibitory concentration) values for compounds 1-3 of lipid peroxidation in rat liver homogenate were 261, 28, 88 μm, respectively. Compounds 1-3 increased superoxide dismutase (SOD) activity with EC50 (50% effective concentration) values of 575, 167, 128 μm, and glutathione peroxidase (GSH-Px) activity with EC50 values of 717, 347, 129 μm, respectively, and showed only weak DPPH (1,1- diphenyl-2-picrylhydrazyl) radical scavenging activity

scholarly journals In VitroAntioxidant and Cytotoxic Activities ofArnebia benthamii(Wall ex. G. Don): A Critically Endangered Medicinal Plant of Kashmir Valley

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Showkat Ahmad Ganie ◽  
Tanveer Ali Dar ◽  
Rabia Hamid ◽  
Ovais Zargar ◽  
Shayaq Ul Abeer ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Aqueous Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Rat Liver Microsomes ◽  
Scavenging Activity

Arnebia benthamiiis a major ingredient of the commercial drug available under the name Gaozaban, which has antibacterial, antifungal, anti-inflammatory, and wound-healing properties. In the present study,in vitroantioxidant and anticancer activity of different extracts ofArnebia benthamiiwere investigated. Antioxidant potential of plant extracts was evaluated by means of total phenolics, DPPH, reducing power, microsomal lipid peroxidation, and hydroxyl radical scavenging activity. The highest phenolic content (TPC) of 780 mg GAE/g was observed in ethyl acetate, while the lowest TPC of 462 mg GAE/g was achieved in aqueous extract. At concentration of 700 µg/mL, DPPH radical scavenging activity was found to be highest in ethyl acetate extract (87.99%) and lowest in aqueous extract (73%). The reducing power of extracts increased in a concentration dependent manner. We also observed its inhibition on Fe2+/ascorbic acid-induced lipid peroxidation (LPO) on rat liver microsomesin vitro. In addition,Arnebia benthamiiextracts exhibited antioxidant effects on Calf thymus DNA damage induced by Fenton reaction. Cytotoxicity of the extracts (10–100 µg/mL) was tested on five human cancer cell lines (lung, prostate, leukemia, colon, and pancreatic cell lines) using the Sulphorhodamine B assay.

scholarly journals In vitro antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum

2019 ◽  
Vol 19 (3) ◽  
pp. 100-108
Author(s):  
K. Thanzami ◽  
B.B. Kakoti ◽  
C. Lalremruati
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Chloroform Extract ◽  
In Vitro Models ◽  
Percent Inhibition ◽  
Anti Inflammatory

The objective of this study was to investigate the antioxidant and anti-inflammatory activities of the chloroform extract of Combretum punctatum var squamosum by in vitro models. Antioxidant activity was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and lipid peroxidation assays. The phenolic and flavonoid contents of the extract were also assessed. The extract at different concentrations showed significant percent inhibition of DPPH and lipid peroxidation and high reducing power when compared with the standard. The extract also showed a significantly high content of phenolics and flavonoids. Anti-inflammatory activity was studied by inhibition of albumin denaturation and human red blood cell (HRBC) membrane stabilization methods. The extract at different concentrations showed significant percent inhibition of albumin denaturation and percent inhibition of haemolysis when compared with the standard. Thus, the result indicates that the chloroform extract exhibited significant potential on anti-inflammatory and antioxidant activities.

scholarly journals Antioxidant Activity Profiling of Acetonic Extract of Jamun (Syzygium cumini L.) Seeds in Different In-Vitro Models

2020 ◽  
Vol 12 (1) ◽  
pp. 3-8
Author(s):  
Neha Yadav ◽  
Ajay Pal ◽  
Sonam Sihag ◽  
Nagesh C.R
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Metal Chelation ◽  
Syzygium Cumini

Background: Syzygium cumini L., commonly known as Jamun, black-plum, and Indian blackberry, is one of the most widely distributed trees in India with booming medical benefits and possesses antioxidant, anticancer and anti-diabetic properties. It belongs to the family Myrtaceae. Despite countless phytochemicals, seeds are not consumed and are the waste part of Jamun fruit. Objective: The objective of this study was to evaluate the antioxidant capacity of phenolics from Jamun seeds against a bundle of oxidant moieties. Methods: The 50% acetone extract of Jamun seeds was investigated for in-vitro antioxidant profiling. Assays include free radical scavenging activity, metal chelation activity, hydroxyl radical scavenging activity, hydrogen peroxide scavenging activity, total antioxidant activity, total reducing power, nitric oxide scavenging activity, and lipid peroxidation inhibition activity. Results: The extract depicted maximum DPPH radical scavenging activity followed by ABTS radical scavenging activity. Hefty metal chelation and nitric oxide scavenging activity were recorded while lipid peroxidation, H2O2, and OH- scavenging activity was intermediate. Conclusion: Jamun seed showed ample antioxidant activity and certifies that it is the right candidate for exploitation as a source of natural antioxidants to counteract autoxidation-induced pathologies or diseases.

Sign in / Sign up

Export Citation Format

Share Document