scholarly journals Preparative Capillary GC for Characterization of Five Dracocephalum Essential Oils from Mongolia, and their Mosquito Larvicidal Activity

2016 ◽  
Vol 11 (10) ◽  
pp. 1934578X1601101
Author(s):  
Gülmira Özek ◽  
Nurhayat Tabanca ◽  
Mohammed M. Radwan ◽  
Sanduin Shatar ◽  
Altaa Altantsetseg ◽  
...  
Keyword(s):  
Essential Oils ◽  
Larvicidal Activity ◽  
System Structure ◽  
Capillary Gc ◽  
Adulticidal Activity ◽  
Fraction Collector ◽  
First Time ◽  
C Nmr

The chemical composition and mosquito larvicidal and adulticidal activity of five essential oils (EOs), Dracocephalum ruyschiana L. (DR), D. foetidum Bunge (DF), D. moldavica L. (DM), D. fruticulosum Steph. ex Willd. (DFr) and D. peregrinum L. (DP) were evaluated. Simultaneous GC-FID and GC-MS analyses revealed in the EOs of DR, DF and DM an unidentified compound (1) ([M+122], 5.4%, 57.9% and 74.0%, respectively). Therefore, we aimed to isolate compound 1 using Preparative Capillary GC (PCGC) connected to a Preparative Fraction Collector (PFC) system. Structure determination of 1 was determined by 1H- and 13C-NMR as p-mentha-1,8-dien-10-al (1, limonen-10-al). Other detected major constituents were thymol (34.0%) and carvacrol (6.1%) in DR; limonene (28.8%) in DF; cis-chrysanthenol acetate (29.1%) and trans-verbenol (5.0%) in DFr; and linalool (17.9%), trans-β-bergamotene (7.9%), (E)-nerolidol (7.7%) and eugenol (5.5%) in DP. The DFr, DP, DR and DM EOs produced 100% mortality to 1st instar larvae of Aedes aegypti L. at 250 ppm and DR and DM EO exhibited the strongest activity and killed 100% 1st instar larvae at 62.5 ppm. No adulticidal activity was observed against female Ae. aegypti. In the scope of the present study, for the first time isolation of limonen-10-al (1) from DR, DM, and DP EOs and larvicidal activity of five Dracocephalum EOs were reported.

scholarly journals Chemical Composition of the Essential Oils from Two Vietnamese Asarum Species: A. glabrum and A. cordifolium

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Tran Huy Thai ◽  
Ophélie Bazzali ◽  
Tran Minh Hoi ◽  
Nguyen Anh Tuan ◽  
Félix Tomi ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Nmr Spectroscopy ◽  
Endangered Species ◽  
Essential Oil ◽  
Essential Oils ◽  
Spectroscopic Techniques ◽  
Red Data Book ◽  
Data Book ◽  
First Time ◽  
C Nmr

The essential oil isolated from Asarum cordifolium C. E. C. Fischer recently discovered in Vietnam, and A. glabrum Merr., an endangered species listed as vulnerable in the Red Data Book of Vietnam, have been analyzed by a combination of chromatographic and spectroscopic techniques including 13C NMR spectroscopy. The composition of A. cordifolium essential oil, investigated for the first time, was dominated by elemicin (82.5%). The essential oil isolated from A. glabrum contained safrole (41.9%) as its major component and was characterized by the diversity of phenylpropanoids contained in this oil (10 compounds).

Determination of Lead Traces by Stripping Voltammetry Using Ti(N,C) Working Electrodes

2010 ◽  
Vol 65 ◽  
pp. 168-173
Author(s):  
Marta Ziemnicka ◽  
Bogusław Baś ◽  
Mateusz Jeż ◽  
Ludosław Stobierski
Keyword(s):  
Electron Microscopy ◽  
Electrode Surface ◽  
Stripping Voltammetry ◽  
Electrochemical Activation ◽  
Trace Metal Analysis ◽  
Response Performance ◽  
Disc Electrodes ◽  
First Time

Electrochemical stripping techniques still attract considerable attention for trace metal analysis and for measuring several important organic compounds, due to their unique capabilities of pre-concentrating the analytes at the electrode surface and associated favourable low limits of detection. In this work it is reported for the first time that the Ti(N0,1C0,9), Ti(N0,4C0,6) and Ti(N0,5C0,5) working disc electrodes, a „mercury-free” sensors, offers surprisingly good analytical performance and some valuable properties. The analytical applicability of the Ti(N,C) electrode was confirmed in determination of lead(II) traces in synthetic solutions both with and without surfactants, in certified reference material and in natural water samples. The effects of dissolved oxygen, acids, anions and metal ions were investigated for the Ti(N,C) electrode. Newly prepared and not activated electrode usually cannot be polarized. Therefore, to use the electrode as a voltammetric sensor, its electrochemical activation is required. Composition of conditioning electrolyte and procedures of the electrodes activation were optimized. The fabrication and the response performance of the investigated electrodes were described in the paper. The voltammetric data were associated with the structural characterization of the electrode surface using scanning electron microscopy (SEM) and transmition electron microscopy (TEM).

scholarly journals The Impact of Geographical Location on the Chemical Compositions of Pimpinella lutea Desf. Growing in Tunisia

2021 ◽  
Vol 11 (16) ◽  
pp. 7739
Author(s):  
Hekmat AL-Hmadi ◽  
Ridha El Mokni ◽  
Rajesh K. Joshi ◽  
Mohamed L. Ashour ◽  
Saoussen Hammami
Keyword(s):  
Essential Oils ◽  
Geographical Location ◽  
Chemical Compositions ◽  
Different Populations ◽  
High Level ◽  
Physical Environmental Factors ◽  
The Impact ◽  
First Time ◽  
North And South

Essential oils are generally produced to confer the protection of medicinal plants against several natural enemies. Variations of chemical and physical environmental factors exert significant influences on plant development. They hence may affect the quality and quantity of volatile organic metabolites of interest and, therefore, the economic applications of essential oils. This research focused on the effects of the harvest region on the production and analytes present in Tunisian Pimpinella lutea Desf. Apiaceae that were collected in three different growing environments (North and South Bizerta and Tabarka). Essential oils extracted from a variety of genotypes were analyzed, for the first time, using gas chromatography and mass spectrometry (GC/FID and GC/MS). The determination of the percentage of essential oil components allowed the recognition of three chemotypes: α-trans-Bergamotene quantified at a percentage of 18.1% in North Bizerta (NBEO), muurola-4,10(14)-dien-1-β-ol identified in South Bizerta (10.1%, SBEO) and acora-3,7(14)-dien present in a high level of 29.1% in Tabarka population (TEO). The richness of different populations in sesquiterpenes (60.2–78.1%) suggests that Pimpinella lutea Desf. may be used in different industrial segments.

scholarly journals Composition of Seed Essential Oils of Rhododendron tomentosum

2012 ◽  
Vol 7 (2) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Asta Judzentiene ◽  
Rita Butkiene ◽  
Jurga Budiene ◽  
Félix Tomi ◽  
Joseph Casanova
Keyword(s):  
Quantitative Analysis ◽  
Chemical Composition ◽  
Nmr Spectroscopy ◽  
Essential Oil ◽  
Essential Oils ◽  
Thermal Isomerization ◽  
Total Oil ◽  
First Time ◽  
Rhododendron Tomentosum ◽  
C Nmr

For the first time, the chemical composition of the seed essential oil of Rhododendron tomentosum was determined. Forty-seven compounds were identified, comprising 91.7% of the total oil. Palustrol (38.3%) and ledol (27.0%) were the predominant constituents. Some constituents, such as β-pinene oxide, iso-menthyl acetate, nerolidyl acetate, cadalene and guaiazulene were characteristic only for the seeds and were identified for the first time in Rh. tomentosum oils. For comparison purposes, the essential oil isolated from the shoots of the same plant were analyzed [GC(FID) in combination with RIs, GC-MS and 13C NMR]. More than a half of the oil was comprised of ledol (36.5%) and palustrol (21.0%). Quantitative analysis of ascaridol, a heat-sensitive compound, was carried out by 13C NMR spectroscopy. Indeed, ascaridol undergoes partial thermal isomerization to iso-ascaridol during GC analyses.

scholarly journals Isolation of eudesmane type sesquiterpene ketone from Prangos heyniae H.Duman & M.F.Watson essential oil and mosquitocidal activity of the essential oils

2018 ◽  
Vol 16 (1) ◽  
pp. 453-467 ◽  
Author(s):  
Gülmira Özek ◽  
Erdal Bedir ◽  
Nurhayat Tabanca ◽  
Abbas Ali ◽  
Ikhlas A. Khan ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
2D Nmr ◽  
Positive Control ◽  
System Structure ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
Fraction Collector ◽  
Mosquitocidal Activity ◽  
One Step

AbstractIn the present work, an endemic species Prangos heyniae collected in four locations from Turkey was subjected to hydrodistillation in Clevenger type apparatus to obtain the essential oils (EO1-4). The gas-chromatography/mass spectrometry (GC/MS) and gas-chromatography-flame ionization detector (GC/FID) analyses showed that the EOs were rich in sesquiterpenes, germacrene D (10.3-12.1%), β-bisabolene (14.4%), kessane (26.9%), germacrene B (8.2%), elemol (3.4-46.9%), β-bisabolenal (1.4-70.7%), β-bisabolenol (8.4%) and an eudesmane type sesquiterpene (1) (16.1%) with [M+218]. This unidentified compound (1) was isolated in a rapid one-step manner with >95.0% purity using Preparative Capillary Gas Chromatography (PCGC) with an HP Innowax column connected to a Preparative Fraction Collector (PFC) system. Structure determination was accomplished from 1D- and 2D-NMR spectroscopic data which determined a new eudesmane type sesquiterpene, 3,7(11)-eudesmadien-2-one (1). Using a biting deterrent bioassay, the mean proportion not biting (PNB) values of the P. heyniae EO1-4 were 0.88 for EO1 and 0.80 for EO2 which were similar to the positive control DEET (N,N-diethyl-3-methylbenzamide). The EO3 and EO4 had lower PNB values of 0.64 and 0.44, respectively. P. heyniae EO1-4 showed good larvicidal activity at 125 and 62.5 ppm whereas EO1-3 were slightly less effective at the dose of 31.25 ppm and EO4 was not active at 31.25 ppm against 1st instar Aedes aegypti.

scholarly journals Antioxidant Activity and Characterization of One New Polysaccharide Obtained fromPerigord Truffle(Tuber huidongense)

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Yong-Feng Chen ◽  
Wen-wen Jiang ◽  
Shi-qi Zhang ◽  
Jian-quan Kan ◽  
Yong Liang
Keyword(s):  
Molecular Structure ◽  
Antioxidant Activity ◽  
Structural Characteristics ◽  
Water Soluble ◽  
Separation And Purification ◽  
Edible Fungus ◽  
Main Components ◽  
First Time

As a medicinal and edible fungus parasitizing on the trees, Perigord Truffle (Tuber huidongense) is well known for its delicious taste, unique smell, and high medical value for healthcare. One new water-soluble nonstarch polysaccharide (PST-W with the yield of 0.41%) fromPerigord Truffle(Tuber huidongense) was purified and identified on structural characteristics for the first time. The characterizations of PST-W were studied on physicochemical properties, main components of monosaccharide(s), and molecular structure. The monosaccharide compositions of PST-W were studied and identified as glucan, only containing D-glucoses with the molecular structure of [→6)α-D-Glcp(1→6)  α-D-Glcp (1→]nby methylation analysis and NMR. In the determination of total reducing capacity, the reducing abilities of polysaccharide extracts could be listed as vitamin C > PST-W > crude polysaccharides-3 > crude polysaccharides-2 > crude polysaccharides-1. All of PST-W, crude polysaccharides-2, and crude polysaccharides-3 were relatively good scavenger for 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl radicals with IC50of 2.81, 4.17, and 3.44 mg/mL, respectively. However,O2-∙clearing abilities of PST-W and crude polysaccharides were obviously weaker. The activities of total crude extract were the worst, indicating that the impurities might negatively affect the antioxidant activity. Thus, the separation and purification of polysaccharides were significant to increase the antioxidant activity in some degree.

Characterization of 10 Biogenic Amines in Male Silkworm Moth by HPLC with Precolumn Derivatization

2020 ◽  
Vol 16 (5) ◽  
pp. 608-614
Author(s):  
Mingqin Fan ◽  
Yun Ai ◽  
Wenjie Zhao ◽  
Yanni Sun ◽  
Jianli Liu ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Biologically Active ◽  
Low Molecular Weight ◽  
Precolumn Derivatization ◽  
Mean Values ◽  
The Mean ◽  
Toxic Potential ◽  
First Time

Background: Biogenic Amines (BAs) are biologically active nitrogenous organic compounds of low molecular weight, which are frequently found in a wide variety of foods, beverages and herbs due to their toxic potential in humans. Male Silkworm Moth (MSM), a Traditional Chinese Medicine (TCM), has been exploited and utilized as nutritious liquor based on its traditional effects in the Chinese community. Objective: The objective of this study was to develop an HPLC with Dns-Cl derivatization method for characterizing overall BAs in MSM and providing data for further evaluating its activities and safety profiles. Methods: The method has acceptable sensitivity, precision, accuracy, selectivity and recovery, and was successfully applied to the determination of the BAs contents in MSM for the first time. Results: In the analysis of 10 batches of MSM samples, serotonin and dopamine were not found in detectable concentrations in any samples, and the most abundant amine found was putrescine. The mean values of tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine determined in the samples were found to be 34.7 mg/ kg, 16.1 mg/ kg, 218.3 mg/ kg, 37.9 mg/ kg, 12.1 mg/ kg, 18.2 mg/ kg, 4.5mg/ kg, and 0.9 mg/ kg, respectively. Conclusion: The contents of BAs in 10 batches of MSM were below the maximum recommended limits, and MSM can be used safely.

Determination of Phenolic Compounds in the Methanolic Extract of Pourouma guianensis Leaves by UPLC-DAD-MS

2020 ◽  
Vol 10 (5) ◽  
pp. 566-570
Author(s):  
Queitilane de S. Sales ◽  
Marcelo da S. Mathias ◽  
Rodrigo R. de Oliveira
Keyword(s):  
Phenolic Compounds ◽  
Formic Acid ◽  
Solvent System ◽  
Efficient Tool ◽  
Phenolic Substances ◽  
Chemical Knowledge ◽  
First Time ◽  
Southeastern Region

Background: The presence of phenolic compounds is common in Urticaceae. However, only one report of the isolation of an apigenin in the Pourouma genus is available. Pourouma guianensis occurs widely throughout Brazil, mainly in the Southeastern region, and is popularly known as “pitinga”. The chemical profile of P. guianensis is marked by the presence of steroids and triterpenes. Objective: This study carried out the characterization of phenolic substances using UPLC-DAD-MS separation, in order to increase the chemical knowledge on the Pourouma genus. Methods: The extract was analyzed by UPLC-DAD-MS using a C-18 column, DAD detector at 190- 400nm and ESI-Q-TOF mass spectrometer. The solvent system consisted of water with 0.1% formic acid (phase A) and acetonitrile with 0.1% formic acid (phase B). Results: The extract analysis identified the presence of ten compounds: two aryl propanoids (5-Ocaffeoylquinic acid and 3-O-feruloylquinic acid) and eight glycosylated flavones derived from apigenin, luteonin, and chrysoeryol (vicenin 2, saponarin, orientin, isoorientin, isoschaftoside, schaftoside, isovitexin, and isoscoparin). Conclusion: The developed UPLC-DAD method was proven to be an efficient tool for the separation of phenolic mixtures and the UPLC-DAD-MS analysis enabled the identification of these compounds, reported for the first time in Pourouma genus.

The conformational properties of seven-membered heterocycles: 1,3-dioxacyclohept-5-ene and its 2-substituted derivatives

1981 ◽  
Vol 59 (15) ◽  
pp. 2283-2289 ◽  
Author(s):  
R. St-Amour ◽  
M. St-Jacques
Keyword(s):  
Low Temperature ◽  
Dynamic Properties ◽  
Substituent Effects ◽  
Dynamic Processes ◽  
Stable Conformation ◽  
Spectral Changes ◽  
Conformational Properties ◽  
C Nmr

1,3-Dioxacycloheptene and its 2-CH3, 2-OCH3, 2-t-Bu, and 2,2-dimethyl derivatives were studied by 1H and 13C dnmr methods. Substituent effects and the interpretation of spectral changes in 13C nmr at low temperature allow the determination of the most stable conformation of these compounds (TB for 1–4b and C for 5b) and the characterization of the dynamic processes observed for 2b, 4b, and 5b. The differences between the conformational and dynamic properties of the above compounds and their benzo analogs are rationalized in terms of different torsional interactions in the two series.

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