scholarly journals A PROCEDURE FOR THE USE OF ATOMIC ABSORPTION SPECTROPHOTOMETRY IN QUANTITATIVE HISTOCHEMISTRY

1974 ◽  
Vol 22 (2) ◽  
pp. 104-109 ◽  
Author(s):  
THOMAS H. ROSENQUIST ◽  
JUDITH W. ROSENQUIST
Keyword(s):  
Atomic Absorption ◽  
Low Cost ◽  
Young Male ◽  
Dry Weight ◽  
Atomic Absorption Spectrophotometry ◽  
Male Rats ◽  
Precipitation Reaction ◽  
Colloidal Iron ◽  
Absorption Spectrophotometry ◽  
Ready Availability

In this quantitative histochemical study, tissues were sectioned, then stained with procedures that conferred a metallic precipitate. Sections were photographed, removed from the slides, air-dried and weighed and then dissolved in acid and the metal content analyzed by atomic absorption spectrophotometry. The stains used in this study were the Alcian Blue (copper phthalocyanin) critical electrolyte concentration procedure on sections of the heart and duodenum from young male rats, colloidal iron on sections of the kidney from adolescent and senescent rats, silver impregnation on sections of the kidney of adolescent and senescent rats and an adenosine triphosphatase lead precipitation reaction on sections of the liver, kidney and heart from adolescent and senescent mice. The objective data obtained by the use of atomic absorption spectrophotometry, reported as micrograms of metal per milligram of dry weight of tissue, allowed statistical analysis of any differences in stain intensity of metallic dyes or metal-precipitating histochemical reactions. Attributes of atomic absorption spectrophotometry which make it desirable for use in this application include accuracy, ease of operation, low cost and ready availability.

Essential Elements in Dry and Canned Butter Beans (Phaseolus limensis L.)

1989 ◽  
Vol 52 (3) ◽  
pp. 204-206
Author(s):  
ANTHONY LOPEZ ◽  
HARRIET L. WILLIAMS
Keyword(s):  
Atomic Absorption ◽  
Dry Weight ◽  
Atomic Absorption Spectrophotometry ◽  
Essential Elements ◽  
Weight Basis ◽  
Element Content ◽  
Absorption Spectrophotometry ◽  
Wet Weight ◽  
Process Element

Ten essential elements were determined in dry and canned butter beans (Phaseolus limensis L.) by atomic absorption spectrophotometry. Samples were taken at different stages during the canning process to determine where changes in element content occurred. The content of each sample was compared statistically to other samples taken within the process. Element retention, excluding chloride and sodium, ranged from 51 to 84% on a dry weight basis, and 14 to 24% on wet weight basis.

The Estimation of Magnesium in Plasma, Muscle and Bone, by Atomic Absorption Spectrophotometry

1969 ◽  
Vol 15 (10) ◽  
pp. 979-996 ◽  
Author(s):  
B J Hunt
Keyword(s):  
Atomic Absorption ◽  
Dry Weight ◽  
Magnesium Content ◽  
Atomic Absorption Spectrophotometry ◽  
Sampling Procedure ◽  
Mean Value ◽  
Male Rats ◽  
Average Weight ◽  
The Mean ◽  
Normal Men

Abstract The use of the EEL 140 atomic absorption spectrophotometer for the determination of magnesium in plasma, skeletal muscle, and bone is described together with an examination of the pattern of chemical interference in each type of sample. The sampling procedure and preparation of each kind of sample for analysis is detailed, and recovery studies on plasma bone and muscle are presented. The mean value for plasma magnesium in 42 normal men and women was 2.16 mg/100 ml ± SD of 0.08. For 30 male Wistar strain rats, the plasma value was 2.10 mg/100 ml ± SD of 0.18. In 15 normal postmortem human muscle samples, the mean magnesium content was found to be 93 mg/100 g of dry muscle, the series range being between 83 and 101 mg/100 g. The comparable value in 49 male rats was 125 mg/100 g dry weight ± SD 1.8. Bone magnesium in femur diaphysis of 20 male rats (average weight 200 g) was 563 mg/100 g dry weight ± SD 12.7. It is concluded that this instrument, when operated in the manner suggested in our study, is technically easy to use, and gives precise, sensitive, and reproducible results for magnesium in a variety of biologic material.

Essential Elements in Dry and Canned Chick Peas (Cicer arietinum L.)

1988 ◽  
Vol 51 (7) ◽  
pp. 574-576 ◽  
Author(s):  
ANTHONY LOPEZ ◽  
HARRIET L. WILLIAMS
Keyword(s):  
Atomic Absorption ◽  
Cicer Arietinum ◽  
Dry Weight ◽  
Atomic Absorption Spectrophotometry ◽  
Essential Elements ◽  
Weight Basis ◽  
Cicer Arietinum L ◽  
Absorption Spectrophotometry ◽  
Wet Weight ◽  
Process Element

Ten essential elements were determined in dry and canned chick peas (Cicer arietinum L.) by atomic absorption spectrophotometry. Samples were taken at different stages during the canning process to determine where changes in element content occurred. The content of each sample was compared statistically to other samples taken within the process. Element retention, excluding chloride and sodium, ranged from 12 to 127% on a dry weight basis and 4 to 45% on wet weight basis.

Levels of hippocampal calcium and zinc following kindling-induced epilepsy

1985 ◽  
Vol 63 (2) ◽  
pp. 159-161 ◽  
Author(s):  
I. Mody ◽  
J. J. Miller
Keyword(s):  
Dentate Gyrus ◽  
Atomic Absorption ◽  
Granule Cells ◽  
Zinc Content ◽  
Dry Weight ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry

Hippocampal calcium and zinc content was determined using atomic absorption spectrophotometry in control and commissural-kindled rats. In animals exhibiting 5–10 consecutive motor seizures hippocampal calcium was slightly elevated (356.7 parts per million (ppm), dry weight) but not significantly different from controls (329.8 ppm), whereas the amount of zinc was significantly higher (101.6 ppm) than in nonstimulated animals (88.3 ppm). These results are indicative of certain pathophysiological changes in kindled hippocampi, most likely localized to the granule cells of the dentate gyrus where the bulk of hippocampal zinc is confined.

Analysis of Foods for Arsenic and Selenium by Acid Digestion, Hydride Evolution Atomic Absorption Spectrophotometry

1977 ◽  
Vol 60 (4) ◽  
pp. 813-825 ◽  
Author(s):  
Milan Ihnat ◽  
Hanford J Miller
Keyword(s):  
Atomic Absorption ◽  
Detection Limits ◽  
Dry Weight ◽  
Atomic Absorption Spectrophotometry ◽  
Transient Signal ◽  
Acid Digestion ◽  
Absorption Spectrophotometry ◽  
Inorganic As ◽  
Entrained Air ◽  
Reference Samples

Abstract A method based on acid digestion, hydride evolution atomic absorption spectrophotometry for estimating microgram and submicrogram quantities of As and Se in foods was developed and evaluated. Samples up to 3 g dry weight were digested with HNO3-HClO4-H2SO4. As and Se in aliquots of the digests were reduced with NaBH4 to volatile hydrides, using laboratory-constructed and commercially available generators. As and Se were estimated by transient signal atomic absorbance measurements as the hydrides were decomposed in an Ar-H2-entrained air flame. Recoveries of inorganic As and Se added at levels of 0.1—1.0 μg/g to a variety of foods ranged from 70 to 125%. Analyses of several standard reference samples indicated the method is capable of recovering native analytes. Detection limits for the determinative step and the method as a whole were as low as 5 and 25 ng, respectively, for both elements.

scholarly journals Content and Accumulation of Oxalic Acid in Edible Species of Xanthosoma.

1969 ◽  
Vol 69 ◽  
pp. 169-176
Author(s):  
Dolores Cuebas de Escabí ◽  
Arturo Cedeño-Maldonado
Keyword(s):  
Oxalic Acid ◽  
Atomic Absorption ◽  
Leaf Blade ◽  
Dry Weight ◽  
Atomic Absorption Spectrophotometry ◽  
Tissue Development ◽  
Progressive Reduction ◽  
Absorption Spectrophotometry ◽  
Edible Species ◽  
Different Tissues

A study was conducted to determine the content and accumulation of oxalic acid in tissues of three edible species of the genus Xanthosoma: X. caracu, X. atrovirens and X. violaceum. Oxalic acid determinations were performed by atomic absorption spectrophotometry. Significant differences were found among the species and tissues studied. Oxalate content was higher in leaf blade and petiole tissues and lower in the pulp of the cormels or rhizomes. Oxalate content in the rhizomes was lowest in X. violaceum and highest in X. caracu. Accumulation in different tissues was progressive and simultaneous with tissue development. No evidence of acid translocation was detected. Postharvest storage of rhizomes resulted in progressive reduction in oxalate content. In all samples analyzed throughout the study the oxalate content of cormels never exceeded 3% of their dry weight.

EFFECTS OF PERFUSION MEDIA ON THE Ca++ AND Mg++ CONTENT OF RAT LIVER

1966 ◽  
Vol 44 (6) ◽  
pp. 893-900 ◽  
Author(s):  
R. A. Hickie ◽  
H. Kalant
Keyword(s):  
Cell Adhesion ◽  
Atomic Absorption ◽  
Rat Liver ◽  
Whole Blood ◽  
Intraperitoneal Injection ◽  
Atomic Absorption Spectrophotometry ◽  
Male Rats ◽  
Hepatic Perfusion ◽  
Absorption Spectrophotometry ◽  
Mg Content

Normal adult male rats were injected with 45CaCl2 intraperitoneally, and radioactivity in whole blood and liver was assayed at intervals of 10 minutes to 48 hours; the results showed rapid equilibration of 45Ca in the liver. In different animals, hepatic perfusion was carried out 4 hours after intraperitoneal injection of 45CaCl2. Portal perfusion with 100 ml of Krebs–Ringer phosphate (KRP) solution removed over 90% of the 45Ca from the liver, and addition of 0.5% EDTA to the solution did not alter the amount removed, although it resulted in earlier 45Ca washout. In other groups of rats, Ca++ and Mg++ concentrations were measured by atomic absorption spectrophotometry in samples of unperfused liver, and livers perfused with various media. Perfusion with KRP had little effect, but KRP + EDTA removed 60% of the Ca++ and 10% of the Mg++, whereas Ca++- and Mg++-free KRP removed 76% of the liver Ca++ but only 4% of the Mg++. It is concluded that tissue Ca++ is extremely loosely bound, and that EDTA effects on cell adhesion and permeability are due primarily to removal of tissue Ca++.

Analysis of Lead Heavy Metal (Pb) Contamination on Matte Lipstick Preparations for Sale in Banda Aceh City Aceh Market

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Rizki Andalia ◽  
Mulia Aria Suzanni ◽  
Rini Rini
Keyword(s):  
Heavy Metal ◽  
Atomic Absorption ◽  
Atomic Absorption Spectrophotometry ◽  
The Body ◽  
Permissible Limit ◽  
The Sun ◽  
Absorption Spectrophotometry ◽  
Pb Contamination ◽  
Body Organ ◽  
Banda Aceh

Lipstick or lip dye is a cosmetic dose that serves to coloring, decorative the lips, as a moisturizing material and protect the lips from exposure the sun to provide optimum results. Lipstick should not contain chemicals such as lead (Pb) because the Pb is a heavy metal that is very dangerous when continuously used on the skin, because it will be absorbed into the blood and attack the body organ causing the onset of disease. According to BPOM that the lead rate on the lipstick does not exceed the permissible limit of 20 mg/kg or 20 ppm.This research aims to know the levels contained in the samples are 4 brands of matte lipstick that are sold in the Aceh market in Banda Aceh City with the method of atomic absorption spectrophotometry (AAS). The results showed that on the 4 brands of lipstick matte contain heavy metal lead (Pb) with a rate still qualified allowed by BPOM  is samples A, B, C, and D, respectively at 0.24 ppm; 0.10 ppm; 2.87 ppm and 1.32 ppm, so that the 4 brands of lipstick matte are still used.

PENURUNAN KONSENTRASI LOGAM Pb2+ DAN Cd2+ PADA LIMBAH CAIR INDUSTRI SASIRANGAN DENGAN METODE FITOREMEDIASI

2015 ◽  
Vol 1 (1) ◽  
pp. 41
Author(s):  
Bella Santa Rossi ◽  
Paryanti Paryanti ◽  
Yuli Ristianingsih ◽  
Abubakar Tuhuloula
Keyword(s):  
Atomic Absorption ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry

Pewarnaan pada proses produksi kain sasirangan menghasilkan limbah yang mengandung logam berat, diantaranya adalah timbal (Pb2+) dan kadmium (Cd2+). Penelitian ini bertujuan untuk mengetahui pengaruh proses fitoremediasi terhadap konsentrasi logam Pb2+ dan Cd2+ dari limbah cair industri sasirangan di Kalimantan Selatan, serta mengetahui pertambahan berat basah eceng gondok. Eceng gondok yang sudah dibersihkan dan diaklimatisasi ditanam dalam reaktor berisi larutan limbah dengan konsentrasi 3% (v/v); 9% (v/v); dan 15% (v/v). Sampel diambil setiap 2 hari sekali sebanyak 100 ml dan dianalisis menggunakan AAS (Atomic Absorption Spectrophotometry). Hasil penelitian ini menunjukkan bahwa konsentrasi logam Pb2+ dan Cd2+ dalam limbah setelah proses fitoremediasi terjadi penurunan. Kadar Pb2+ pada konsentrasi 3% (v/v) semula 0,197 ppm menjadi 0,062 ppm, pada konsentrasi 9% (v/v) semula 0,200 ppm menjadi 0,077 ppm dan untuk 15% (v/v) adalah 0,225 ppm menjadi 0,093 ppm. Sedangkan untuk Cd2+ konsentrasi 3% (v/v) semula 0,110 ppm menjadi 0,059 ppm, konsentrasi 9% (v/v) adalah 0,127 ppm menjadi 0,045 ppm dan untuk konsentrasi 15% (v/v) semula 0,144 ppm menjadi 0,047 ppm.

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