In Vitro Antioxidant Activities of the Aqueous and Methanolic Stem Bark Extracts of Piliostigma thonningii (Schum.)

Oxidative stress has been recognized as a key driver of many ailments affecting humankind. Free radicals attack biologically important biomolecules, impairing their functioning, thereby initiating and exacerbating diseases. As a comeback, antioxidant therapies have been proposed as novel approaches to ameliorating oxidative stress–associated diseases including chronic ones. Antioxidants are thought to employ multifaceted and multitargeted mechanisms that either restore oxidative homeostasis or prevent free radical buildup in the body, which overwhelm the endogenous defenses. Plants have been used for many ages across time to manage human diseases, and have a host of antioxidant phytocompounds. Piliostigma thonningii is traditionally used for the management of inflammation, malaria fever, rheumatism, and insanity, among other diseases caused by a disturbed redox state in the body. In this study, in vitro antioxidant activities of the methanolic and aqueous stem bark extracts of P. thonningii were evaluated using the in vitro antilipid peroxidation, the 1,1-diphenyl-2-picryhydrazyl (DPPH) free radical scavenging, and the ferric reducing antioxidant power assay methods. The obtained results revealed remarkable antioxidant activities of the studied plant extracts as evidenced by the low IC50 and EC50 values. These antioxidant activities could be due to the presence of antioxidant phytochemicals like flavonoids, carotenoids, tannins, and phenols, among others. Therefore, the therapeutic potency of this plant could be due to its antioxidant properties. This study recommends in vivo antioxidant efficacy testing of the studied plant extracts, as well as isolation and characterization of bioactive antioxidant compounds that are potent against oxidative stress.

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In vivo and In vitro Antioxidant Activities of Aqueous and Ethanol Stem Bark Extracts of Vitex doniana on Doxorubicin-induced Oxidative Stress in Rats

Asian Journal of Research in Biochemistry ◽

10.9734/ajrb/2021/v8i230178 ◽

2021 ◽

pp. 44-55

Author(s):

Mohammed Aliyu Sulaiman ◽

Daniel Dahiru ◽

Mohammed Auwal Ibrahim ◽

Ahmed Ibrahim Hayatu

Keyword(s):

Oxidative Stress ◽

Antioxidant Activities ◽

Ischemia Reperfusion ◽

Oral Treatment ◽

Stem Bark ◽

Albino Rats ◽

Associated Diseases ◽

Vitex Doniana

Background: Oxidative stress is involved in the pathogenesis of hypertension, myocardial ischemia-reperfusion injury, atherosclerosis, muscular dystrophy, aging and other associated diseases. Vitex doniana is used in Adamawa, northern Nigeria to treat oxidative stress associated diseases. However, the antioxidative effects of the plant have not been scientifically examined in oxidative stress experimental animal models. The aim of this study is to investigate the in vitro and in vivo antioxidant activities of aqueous and ethanol stem bark extracts of Vitex doniana in oxidative stress model of rats. Methods: The study used 35 adult albino rats weighing 175 ± 25 g, of which 30 were induced with oxidative stress by intraperitoneal injection of doxorubicin (10 mg/kg) for three consecutive days. Animals were treated by oral administration of silymarin (100 mg/kg) and Vitex doniana aqueous or ethanol extract (100 mg/kg and 200 mg/kg) for 14 consecutive days before they were sacrificed on the 15th day and blood was analyzed for biochemical indices of oxidative stress. Results: The results of the phytochemistry showed the presence of alkaloids, tannins, flavonoids, steroids, phenols, saponins, terpenoids, glycosides: and total flavonoids (52.70 ± 1.60 mg/ml and 75.40 ± 0.80 mg/ml), total phenols (21.45 ± 1.54 mg/ml and 26.50 ± 1.22 mg/ml) for aqueous and ethanol stem bark extracts respectively. The extracts scavenged DPPH radical, reduced Fe3+ and inhibited lipid peroxidation. Doxorubicin significantly (p<0.05) lowered the levels of SOD, CAT, GR and TAS and significantly (p<0.05) but, increased the level of LPO. Oral treatment with Vitex doniana extracts significantly (p<0.05) increased the activities of CAT, GR, SOD and TAS while LPO was significantly (p<0.05) lowered. Vitex doniana stem bark extracts significantly (p<0.05) improved the biochemical derangements observed in the induced untreated animals in comparable manner to that of Silymarin. Conclusion: The present study provides the scientific rationale for the use of Vitex doniana stem bark in traditional medicine and has a viable antioxidative capacity both in vitro and in vivo.

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Inhibition of Digestive Enzymes, Antioxidant and Free Radical Scavenging Capacities of Stem Bark Extracts of Coula edulis Baill (Olacaceae)

Journal of Food Research ◽

10.5539/jfr.v10n5p1 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1

Author(s):

Eric Beyegue ◽

Boris G. K. Azantsa ◽

Angie M-A Mbong ◽

Julius E. Oben

Keyword(s):

Oxidative Stress ◽

Free Radical ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Stem Bark ◽

Alpha Amylase ◽

In Vitro Assays ◽

Oh Radicals

Prolonged hyperglycemia enhances oxidative stress. Bioactive compounds extracted possess antioxidant, anti-free radical potentials or the ability to reduce blood sugar levels. The objective of this study was to evaluate the antioxidant properties of extracts of stem bark of Coula edulis Baill., their abilities to trap free radicals and glucose, as well as their ability to inhibit α-amylase and invertase activities. In vitro assays were used to test the trapping capacity of extracts on DPPH, ABTS, NO, and OH radicals; to evaluate the antioxidant capacity, the activity of glycosylation and the capacity of inhibition of the activities of α-amylase and invertase were conducted. Also, phenolic, flavonoid and alkaloid contents of extracts were determined. Results showed that extracts of the stem bark of C. edulis have anti-radical properties. The extracts chelate DPPH, hydroxyl (OH), nitrite oxide (NO), ABTS radicals, and even glucose. The IC50 values varied depending on the nature of the extraction solvent. Ethanolic extract has the highest polyphenolic content (289.12 ± 30.31 µg catechin equivalent/g), flavonoids (1.12 ± 0.09 µg quercetin equivalent/g) and alkaloids (5.54 ± 0.59 µg quinin equivalent/g). The extracts also reduce invertase and α-amylase activities. C. edulis extracts present strong antioxidant potentials and can be used as a source of natural antioxidants for the prevention of oxidative stress and hyperglycemia.

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In Vitro Antioxidant Activities of Methanolic Extracts of Caesalpinia volkensii Harms., Vernonia lasiopus O. Hoffm., and Acacia hockii De Wild

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2020/3586268 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Beatrice Muthoni Guchu ◽

Alex King’ori Machocho ◽

Stephen Kiruthi Mwihia ◽

Mathew Piero Ngugi

Keyword(s):

Oxidative Stress ◽

Therapeutic Potential ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antioxidant Power ◽

Chain Reactions ◽

Methanolic Extracts ◽

Ferric Reducing Antioxidant Power

Oxidative stress is the result of the disparity between pro-oxidants and antioxidants in an organism, and it is important in the pathogenesis of several degenerative disorders, such as arthritis, Alzheimer’s, cancer, and cardiovascular diseases. Free radicals can damage biomolecules, such as nucleic acids, lipids, proteins, polyunsaturated fatty acids, and carbohydrates, and the DNA leading to mutations. The use of antioxidants is effective in delaying the oxidation of biomolecules. Antioxidants are complexes found in the food that can retard or deter oxidation by preventing the initiation and propagation of oxidizing chain reactions. Medicinal plants have been used for centuries by man to manage diseases and have a host of antioxidant complexes. Traditionally, Caesalpinia volkensii, Vernonia lasiopus, and Acacia hockii have folkloric remedies against associated oxidative stress-mediated complications. However, the upsurge in its use has not been accompanied by scientific validations to support these claims. In this study, in vitro antioxidant activity of Caesalpinia volkensii, Vernonia lasiopus, and Acacia hockii collected from Embu County (Kenya) were determined by radical scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical in addition to ferric reducing antioxidant power analyzed against that of L-ascorbic acid as the standard. The obtained results revealed remarkable antioxidant activities of the studied plant extracts as evidenced by the low IC50 and EC50 values. These antioxidant activities could be due to the presence of antioxidants phytochemicals such as flavonoids, phenols, terpenoids, and saponins among others. Therefore, the therapeutic potential of this plant could be due to their antioxidant properties. This study recommends bioassay of the extracts against oxidative stress-related disorders for development of phytomedicine with antioxidant properties.

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Antioxidant Activity and Hepatoprotective Potential ofPolyalthia longifoliaandCassia spectabilisLeaves against Paracetamol-Induced Liver Injury

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/561284 ◽

2012 ◽

Vol 2012 ◽

pp. 1-10 ◽

Cited By ~ 20

Author(s):

Subramanion L. Jothy ◽

Azlan Aziz ◽

Yeng Chen ◽

Sreenivasan Sasidharan

Keyword(s):

Free Radical ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Hepatoprotective Activity ◽

Free Radical Scavenging ◽

Dependent Manner ◽

Leaf Extracts ◽

Antioxidant Power ◽

Nitric Oxide Radical

In the present study,in vitroantioxidant, free radical scavenging capacity, and hepatoprotective activity of methanol extracts fromPolyalthia longifoliaandCassia spectabiliswere evaluated using establishedin vitromodels such as ferric-reducing antioxidant power (FRAP), 2,2-diphenyl-1-picryl-hydrazyl(DPPH•), hydroxyl radical(OH•), nitric oxide radical(NO•)scavenging, metal chelating, and antilipidperoxidation activities. Interestingly, all the extracts showed considerablein vitroantioxidant and free radical scavenging activities in a dose-dependent manner when compared to the standard antioxidant which verified the presence of strong antioxidant compound in leaf extracts tested. Phenolic and flavonoid content of these extracts is significantly correlated with antioxidant capacity. SinceP. longifoliaextract was exhibited betterin vitroantioxidant activities, it was subjected forin vivohepatoprotective activity in paracetamol-intoxicated mice. Therapy ofP. longifoliashowed the liver protective effect on biochemical and histopathological alterations. Moreover, histological studies also supported the biochemical finding, that is, the maximum improvement in the histoarchitecture of the liver. Results revealed thatP. longifolialeaf extract could protect the liver against paracetamol-induced oxidative damage by possibly increasing the antioxidant protection mechanism in mice. Our findings indicated thatP. longifoliaandC. spectabilishave potential as good sources of natural antioxidant/antiaging compounds.

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In vitro Antioxidant Activities and Effect of Hydroethanolic and Aqueous Extracts of Terminalia avicennioides (Combretaceae) on Salmonella

Microbiology Research Journal International ◽

10.9734/mrji/2020/v30i130185 ◽

2020 ◽

pp. 1-14

Author(s):

Louis-Claire Ndel Famen ◽

Benjamin Tangue Talom ◽

Richard Simo Tagne ◽

Gabriel Tchuente Kamsu ◽

Norbert Kodjio ◽

...

Keyword(s):

Typhoid Fever ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Public Health Problem ◽

Stem Bark ◽

Poor Quality ◽

Antioxidant Power ◽

Good Ability

Today, Typhoid fever remains a public health problem in developing countries due to the poor quality of lifestyle associated with abusive and inappropriate use of antibiotics. Aims: Considering the ethnopharmacological relevance of Terminalia avicennioides (T. avicennioides) (Combretaceae), this study was designed to investigate the in vitro antisalmonella and antioxidant activities of various extracts of this plant. Methodology: The microdilution method was used to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of T. avicennioides extract. These extracts were also subjected to in vitro antioxidant tests such as diphényl-2-picrylhydrazyle (DPPH) radical scavenging test, ferric reducing-antioxidant power (FRAP), hydroxyl radical (OH) nitric oxide (NO) and Hydrogen Peroxide Scavenging Capacity. Results: In vitro antisalmonella activity reveals that T. avicennioides stem bark extracts presented MIC values ranging from 64 to 512 μg / mL on tested microorganisms. This extract exhibited a good ability to trap DPPH with an IC50 of 8.30 μg / mL. The iron reducing power obtained with this extract had ODs ranging from 0.96 to 1.63. Phytochemical screening showed the presence of alkaloids, flavonoids, saponins, phenols anthocyanin and anthraquinone in all the extracts. Conclusion: The results suggest that stem extract of T. avicennioides contains antisalmonella and antioxidant substances, which could be used for the treatment of typhoid fever and another salmonellosis.

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In vitro antileishmanial and antioxidant activities of essential oils from different parts of Murraya paniculata (L.) Jack: a species of Rutaceae that occur in the Cerrado biome in Brazil

Australian Journal of Crop Science ◽

10.21475/ajcs.20.14.02.p1966 ◽

2020 ◽

pp. 347-353

Author(s):

Flávia Fernanda Alves da Silva ◽

Cassia Cristina Fernandes ◽

Gabriel Almeida de Oliveira ◽

Ana Carolina Bolela Bovo Candido ◽

Lizandra Guidi Magalhães ◽

...

Keyword(s):

Free Radical ◽

Essential Oils ◽

Antioxidant Activities ◽

Folk Medicine ◽

Natural Antioxidants ◽

The Body ◽

Antioxidant Compounds ◽

Stable Free Radical ◽

Murraya Paniculata

In Brazilian folk medicine, Murraya paniculata has been used for treating intestine disorders, rheumatism and cough. This paper aims to investigate the in vitro antileishmanial and antioxidant activities of essential oils (EO) from M. paniculata leaves and fruits (ripe and unripe ones). Natural antioxidants may be very beneficial to improve quality of life, since they are capable of protecting the body against damage caused by free radicals and, consequently, either preventing or postponing many diseases from starting their cycles. One of the techniques which has been widely used for detecting antioxidant compounds is the method based on the elimination of the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•). It has been considered easy, precise, fast, simple, economical and appropriate to determine antioxidant activity of pure substances and complex mixtures, such as EO. Thus, antioxidant potential of EO was evaluated by using the method of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•). EO from M. paniculata leaves and fruits exhibited weak potential, since EC50 values were above 700 µg/mL. Several reports have stated that volatile oils from plants show promising leishmanicidal activity against promastigote forms of Leishmania amazonensis; in relation to this activity, leaf oil was highly active (IC50 = 7.33±2.07 µg/mL) while ripe and unripe fruit oils were active, with values of IC50 = 30.77±2.07 µg/mL and 13.04±1.64 µg/mL, respectively. Both GC-FID and GC-MS analyses revealed that the major components determined in EO from M. paniculata were sesquithujene (25.0%), trans-β-caryophyllene (23.8%), α-zingiberene (21.0%), α-ylangene (13.3%), germacrene D (13.1%), α-copaene (12.7%), and β-cubebene (10.2%). In vitro antileishmanial and antioxidant activities of EO from M. paniculata have also been described for the first time.

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Antioxidant Activities of Ficus elastica Leaves Ethanol Extract and Its Compounds

Molecular and Cellular Biomedical Sciences ◽

10.21705/mcbs.v4i1.86 ◽

2020 ◽

Vol 4 (1) ◽

pp. 27

Author(s):

Chrismis Novalinda Ginting ◽

I Nyoman Ehrich Lister ◽

Ermi Girsang ◽

Dewi Riastawati ◽

Hanna Sari Widya Kusuma ◽

...

Keyword(s):

Free Radicals ◽

Free Radical ◽

Antioxidant Activities ◽

Rubber Tree ◽

The Body ◽

Moderate Activity ◽

Scavenging Activity ◽

Antioxidant Power ◽

Antioxidant Agents ◽

Eleutheroside B

Background: The excessive free radicals condition called oxidative stress can harmful for the body. To prevent and cure it, the antioxidant agents are required. Nowadays, the natural product extracted from plants have been widely used in folk medicine as antioxidant for the treatment of many diseases. Ficus elastica (rubber tree) has some compounds that has several biological activities, i.e., quercitrin, myricitrin, morin, and eleutheroside B. The F. elastica works against the free radicals and can be potential as antioxidant agent. The purpose of this study was to evaluate antioxidant properties of F. elastica ethanolic extract (FEE), quercitrin, myricitrin, morin, and eleutheroside B.Materials and Methods: The antioxidant activities of FEE and standard compounds were evaluated by free radical-scavenging activity of 2,2-diphenyl-1-picrylhydrazil (DPPH), hydrogen peroxide (H2O2), 2,2’-azinobis-(3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) activities using spectrophotometry method.Results: FEE has the lowest of DPPH scavenging activity (IC50=13.82 µg/mL) than other compounds. In ABTS scavenging activity, FEE has moderate activity with IC50 value 23.29 µg/mL. In FRAP activity, FEE has moderate activity with value 241.58 µM Fe(II)/µg, while in H2O2 scavenging activity, FEE also show moderate activity with IC50=83.97 µg/mL compared to other compounds.Conclusion: In summary, FEE and the pure compounds (quercitrin, myricitrin, morin, and eleutheroside B) have potential as antioxidant agent.Keywords: free radical, morin, myricitrin, quercitrin, rubber tree, scavenging activities

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Free radicals and antioxidants in two oxidative-stress cell models exposed to ochratoxin A and amyloid β: unexpected results

World Mycotoxin Journal ◽

10.3920/wmj2010.1221 ◽

2010 ◽

Vol 3 (3) ◽

pp. 257-261 ◽

Cited By ~ 2

Author(s):

S. Costa ◽

R. Cervellati ◽

E. Speroni ◽

M. Guerra ◽

E. Greco

Keyword(s):

Oxidative Stress ◽

Free Radical ◽

Ochratoxin A ◽

Amyloid Β ◽

Free Radical Scavengers ◽

Cell Models ◽

Antioxidant Power ◽

Species Production ◽

Significant Chemical

Oxidative stress is reported to be closely related to pathogenetic mechanisms and plays a central role in several molecular toxicities. It seems reasonable to hypothesize that antioxidant molecules and/or free radical scavengers can protect from oxidative stress-induced damage by quenching free-radical reactions. Leontopodic acid (LA) is a fully substituted, hexaric acid derivative isolated from Leontopodium alpinum Cass., commonly known as ‘Edelweiss’ (Asteraceae family), and exhibits significant chemical antioxidant power. The present study was designed to evaluate LA’s capacity to counteract induced oxidative stress in two in vitro cell models: one exposed the LLC-PK1 cell line to ochratoxin A and the other exposed the differentiated SH-SY5Y cells to amyloid β. The effects of a 24 h pretreatment with LA on induced ROS (reactive oxygen species) production and cytotoxicity were evaluated in the two models. Surprisingly, the results showed that while LA decreased ROS production, it did not increase cell survival.

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Phytochemicals, Total Phenolic and Flavonoid Contents and Radical Scavenging Activities of Crotalaria Retusa, Clerodendrum Volubile Leaf Extracts and Biogas Slurry of Cassava Peels

10.21203/rs.3.rs-638563/v1 ◽

2021 ◽

Author(s):

Oluwaseyi Olaniyan ◽

Ebenezer Ajayi ◽

Oluwaseun Oladele ◽

Simeon Adenrele ◽

Johnson Oladele ◽

...

Keyword(s):

Oxidative Stress ◽

Free Radical ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Total Phenolic ◽

Biogas Slurry ◽

Promising Therapeutic Approach ◽

Phenolic And Flavonoid

Abstract Well established are the destructive effects of cellular oxidative damage due to oxidative stress on animal and human health. The prophylactic use of plant-derived bioactive agents in the amelioration and treatment of chronic diseases induced by free radical and oxidative stress is a promising therapeutic approach. This present study investigates the phytochemical constituents, in vitro antioxidant and free radical scavenging abilities of Crotalaria retusa leaves, Clerodendrum volubile leaves and cassava biogas slurry. The total phenolic and flavonoid contents of the extracts were also determined. The phytochemicals, phenolic and flavonoid contents and antioxidant potentials of the extracts, including reducing power, inhibition of hydrogen peroxide, hydroxyl radical production, and DPPH radical scavenging, were performed using standard assays. Our results show terpenoids, saponins, cardiac glycosides, flavonoids and tannins in the various extracts. In vitro, antioxidant assay results also reveal that the extracts' free radical scavenging abilities are significantly high compared with standard antioxidants used: tannic and ascorbic acids. The results also show that the extracts demonstrated elevated antioxidant activities positively correlated with the total flavonoid and phenolic contents. This work demonstrated that the extracts possessed significant antioxidant activities over standard ones, thus, suggesting the extracts as sources of natural antioxidants that could have considerable therapeutic relevance in preventing and inhibiting the progression of diseases associated with oxidative stress.

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ANTIOXIDANT ACTIVITY OF ALKALOID FRACTIONS OF ZANTHOXYLUM ACANTHOPODIUM DC. FRUITS WITH 1,1-DIPHENYL-2-PICRYLHYDRAZYL ASSAY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11s1.26560 ◽

2018 ◽

Vol 11 (13) ◽

pp. 33

Author(s):

Rosidah Rosidah ◽

Poppy Anjelisa Zaitun Hasibuan ◽

Ginda Haro ◽

Puteri Masri ◽

Denny Satria

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Free Radical ◽

Antioxidant Activities ◽

Ethanol Extract ◽

Liquid Extraction ◽

The Body ◽

Antioxidant Potential ◽

Liquid Liquid Extraction

Objective: Antioxidants are vital substances which possess the ability to protect the body from damage caused by free radical-induced oxidative stress. The purpose of this study is to determine antioxidant activities of alkaloid fractions of Zanthoxylum acanthopodium DC. fruits.Methods: Z. acanthopodium DC. fruit was extracted by maceration. Ethanol extract was fractionated with liquid-liquid extraction using n-hexane and chloroform at pH 3, 7, 9, and 11 to obtained alkaloid fractions. Antioxidant activity for extract and fractions was determined with 1,1-diphenyl-2- picrylhydrazyl.Results: The IC50 of extract and fractions was 101.11±0.11, 273.24±0.12, 116.62±0.04, 61.12±0.01, 23.15±0.01, and 112.39±0.04 μg/mL, respectively.Conclusion: The results reveal that alkaloid fractions of Z. acanthopodium DC. fruit have very strong antioxidant potential. Our further study is to isolate the alkaloid compounds.

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