Impact on Short and Long-Term Hematopoiesis of G-CSF Administered Posttransplant in Function of the Amounts of Peripheral Blood CD34+38− Cells Reinfused.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 5197-5197
Author(s):  
Philippe R.G. Henon ◽  
Hanna Sovalat ◽  
Mario Ojeda-Uribe ◽  
Yazid Arkam ◽  
Jean-Claude Eisenmann
Keyword(s):  
Group Iv ◽  
The Other ◽  
Group Iii ◽  
Peripheral Blood Cd34 ◽  
Group I ◽  
Cd34 Cells ◽  
Autologous Pbsct ◽  
Short And Long Term ◽  
A Prospective Study

Abstract We previously suggested that a reinfused threshold dose of CD34+38− cells = 5x104/kg b.w. better predict both short- and long-term engraftment after PBSCT than total CD34+ cells assessment, and should thus avoid unecessary postransplant (Tx) G-CSF administration. Therefore, we have further conducted a prospective study comparing postTx data from cancer patients undergoing autologous PBSCT and were administered or not G-CSF depending on the amount of CD34+38− cells reinfused. 48 patients (mean age 49y) were transplanted with, on average 2.5x104 CD34+38− cells/kg b.w. (range 1–49) and were consequently administered G-CSF 5 μg/kg daily from d5 to ANC = 109/1 (Group-I). 46 patients (mean age 50y) received an average of 20.5x104 CD34+38− cells/kg (range 5.5–162) without postTx G-CSF (Group-II). These 2 groups were compared and paired two by two with 2 groups of "historical" patients referred as controls : 11 patients (mean age 44y) had received, on average, 2.5x104 CD34+38− cells/kg b.w. (range 1.1–4.8) without G-CSF (Group-III); 29 patients (mean age 51y) received an average of 15.2x5x104 CD34+38− cells/kg b.w. (range 5.5–60) systematically associated with G-CSF for protocolar reasons (Group-IV). PostTx trilineage hematopoietic engraftment (up to 2 years), clinical and economical parameters were systematically recorded for each group of patients and statistically compared. PostTx ANC recovery occurred sooner, was faster and reached higher levels in the G-CSF groups (II and IV) compared to the others; platelets recovery kinetics was significantly faster in Group-III compared to the others; reticulocytic recovery was not statistically different whichever the group. When age, sex, disease, TBI did not significantly influence trilineage engraftment, a multiparametric study showed strong positive impacts of total CD34+ cells reinfused on ANC kinetics and of CD38− subset amounts on platelet kinetics, which was on the contrary slowered by G-CSF administration. Group-I patients received more transfusions, stayed longer hospitalized and costed more than those of the 3 other groups. Regarding long-term hematopoiesis, platelets and hemoglobin levels were globally higher in Group-III compared to the other groups, but still more dramatically compared to Group-IV from 1 to 9 months, which might be explained by differences in BM CD34+ and 38− subset differentiation. In conclusion, if postTx G-CSF certainly accelerates ANC recovery, it seems to be to the detriment of short- an d median-term platelets and hemoglobin recovery, even in case of reinjection of CD34+38− cell doses = 5x104/kg b.w., which appears thus to be significantly discriminant for G-CSF administration decision.

scholarly journals Grouping of Salmonella enterica serotype Montevideo strains by ribotyping and IS200 profiling

2000 ◽  
Vol 124 (3) ◽  
pp. 375-382 ◽  
Author(s):  
D. C. OLD ◽  
S. A. CHISHOLM ◽  
P. B. CRICHTON ◽  
A. TAYLOR
Keyword(s):  
Salmonella Enterica ◽  
Group Iv ◽  
The Other ◽  
Clonal Structure ◽  
Group Iii ◽  
Group I ◽  
Combined Use ◽  
Group Ii ◽  
The Uk

One-hundred and twenty-one isolates of Salmonella enterica serotype Montevideo, representing different biotypes and incidents of infection detected in the UK between 1977 and 1995, were analysed by EcoRI ribotyping, PvuII ribotyping and IS200 fingerprinting. Among the isolates examined, 7 EcoRI ribotypes, 5 PvuII ribotypes and 55 IS200 profile types were recognized and 4 arbitrary groups defined. All 33 isolates of biotype 2d belonged to EcoRI/PvuII ribotype 1/1 and IS200 lineage A and comprised Group I. The other 88 isolates of biotype 10di and its variants were assigned to Groups II–IV. All 27 isolates in Group II were of EcoRI/PvuII ribotype 2/2 and IS200 lineage B. Among the 43 isolates in Group III, 42 of which were of EcoRI/PvuII ribotype 3/3, IS200 analysis identified 38 profiles in lineages C–I. Six EcoRI/PvuII ribotypes and 8 IS200 profiles, mostly in lineages C–E, were recognized among the 18 isolates in Group IV. The combined use of biotyping and ribotyping, and to some extent IS200 profiling, has enhanced our understanding of the clonal structure of serotype Montevideo and provides a basis for further study.

scholarly journals Short- and Long-Term Impact of Smoking Donors in Lung Transplantation: Clinical and Pathological Analysis

2021 ◽  
Vol 10 (11) ◽  
pp. 2400
Author(s):  
Marco Schiavon ◽  
Andrea Lloret Madrid ◽  
Francesca Lunardi ◽  
Eleonora Faccioli ◽  
Giulia Lorenzoni ◽  
...  
Keyword(s):  
Lung Transplantation ◽  
Continuous Variable ◽  
The Other ◽  
Smoking History ◽  
Group Iii ◽  
Group I ◽  
Donor Factors ◽  
Long Term Impact ◽  
The Impact

Background: The use of smoking donors (SD) is one strategy to increase the organ pool for lung transplantation (LT), but the benefit-to-risk ratio has not been demonstrated. This study aimed to evaluate the impact of SD history on recipient outcomes and graft alterations. Methods: LTs in 293 patients were retrospectively reviewed and divided into non-SD (n = 225, group I), SD < 20 pack-years (n = 45, group II), and SD ≥ 20 pack-years (n = 23, group III) groups. Moreover, several lung donor biopsies before implantation (equally divided between groups) were evaluated, focusing on smoking-related lesions. Correlations were analyzed between all pathological data and smoking exposure, along with other clinical parameters. Results: Among the three groups, donor and recipient characteristics were comparable, except for higher Oto scores and age in group III. Group III showed a longer intensive care unit (ICU) and hospital stay compared with the other two groups. This finding was confirmed when SD history was considered as a continuous variable. However, survival and other mid- and long-term major outcomes were not affected by smoking history. Finally, morphological lesions did not differ between the three groups. Conclusions: In our study, SDs were associated with a longer post-operative course, without affecting graft aspects or mid- and long-term outcomes. A definition of pack-years cut-off for organ refusal should be balanced with the other extended criteria donor factors.

scholarly journals The Effect of Elevated Basal Follicle Stimulating Hormone Levels on Assisted Reproductive Technology Cycle Outcomes

2019 ◽  
pp. 1
Author(s):  
Inci Kahyaoglu ◽  
Hatice Yilmaz Dogru ◽  
Sezin Ertürk Aksakal ◽  
Iskender Kaplanoglu ◽  
Serdar Dilbaz ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Follicle Stimulating Hormone ◽  
Group Iv ◽  
Pregnancy Rates ◽  
The Other ◽  
Hormone Levels ◽  
Group Iii ◽  
Group I ◽  
Significant Difference ◽  
Stimulating Hormone

<p><strong>OBJECTIVE:</strong> Despite the availability of better biomarkers, basal day 3 follicle-stimulating hormone is widely available and often used as the first-line test in ovarian reserve evaluation. The aim of this study was to evaluate the outcomes of cycles with elevated (&gt;12 IU/mL) basal follicle-stimulating hormone values. </p><p><strong>STUDY DESIGN:</strong> Cycles with basal day 3 follicle-stimulating hormone values &gt;12 IU/mL were divided into four cohorts according to follicle-stimulating hormone levels: group I, follicle-stimulating hormone between 12-15 IU/m, group II between 15-20 IU/mL, group III between 20-25 IU/mL and group IV &gt;25 IU/mL. Both demographic characteristics and controlled ovarian stimulation parameters were retrospectively reviewed. </p><p><strong>RESULTS:</strong> Total antral follicle count was significantly higher in group I compared to the other three groups (p=0.001). Number of follicles ≥17 mm on human chorionic gonadotropin (hCG) day, number of retrieved oocytes, mature oocytes and fertilized oocytes were significantly higher in group I compared to the other groups (p=0.003, p=0.001, p=0.001, and p=0.001, respectively). No significant difference was found between groups regarding cancellation rates. The rate of embryo transfer per started cycle was significantly higher in group I compared to group III and group IV (p=0.001). Clinical pregnancy rates per embryo transfer were comparable among the groups.</p><p><strong>CONCLUSION:</strong> Despite the retrieval of lower quantities of oocytes, reasonable pregnancy rates could be achieved if embryo transfer was performed in cycles with follicle-stimulating hormone values over 12 IU/mL.</p>

scholarly journals The Effects of Strain and Caponisation on Carcass and Meat Traits of Cockerels Aged Twenty Weeks

2016 ◽  
Vol 16 (4) ◽  
pp. 1227-1239 ◽  
Author(s):  
Marek Adamski ◽  
Joanna Kuźniacka ◽  
Mirosław Banaszak
Keyword(s):  
Physicochemical Properties ◽  
Rhode Island ◽  
New Hampshire ◽  
Fat Content ◽  
Group Iv ◽  
The Other ◽  
V Protein ◽  
Weight Percentage ◽  
Group Iii ◽  
Group I

Abstract The analysis of slaughter yield and meat quality was performed on a total number of 96 birds from four medium-heavy weight pedigree strains. Based on their strain, cockerels were divided into the following groups: group I - strain N88 (originating from New Hampshire), group II - strain R55 (Rhode Island Red), group III - strain S11 (Sussex) and group IV - strain P55 (Plymouth Rock). Each group consisted of 24 birds. At 12 weeks of age, half of the cockerels from each group was caponised. In total, 48 birds were caponised (12 birds in each strain). Based on the strain, capons were then divided into groups V (N88), VI (R55), VII (S11) and VIII (P55). It was noted that the strain and caponisation had influence on differences in such traits as the weight of eviscerated carcass with neck and slaughter yield. On the other hand, it was observed that caponisation did not affect significantly the total weight, percentage share of breast and drumstick muscles in carcasses of birds from analysed strains. No significant differences were observed between groups in terms of physicochemical properties of meat. The highest content of water in breast and drumstick muscles was observed among cockerels and capons from strain N88 (groups I and V). Protein content in cockerels’ breast muscles differed depending on their strain whilst among capons (groups V-VIII) it was similar. It was also noted that capons originating from strains S11 (VII) and P55 (VIII) stood out significantly with higher fat content in breast muscles compared to cockerels from the same strain (groups III and IV). The effect of caponisation on higher fat content in drumstick muscles was confirmed in all observed groups. Capons from all strains had more fat in drumstick muscles compared to cockerels of the same origin.

scholarly journals Clinical relevance of a degree of extracapsular extension in a sentinel lymph node in breast cancer patients: a single-centre study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tomasz Nowikiewicz ◽  
Andrzej Kurylcio ◽  
Iwona Głowacka-Mrotek ◽  
Maria Szymankiewicz ◽  
Magdalena Nowikiewicz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Term Treatment ◽  
Extracapsular Extension ◽  
Group Iii ◽  
Metastatic Lesions ◽  
Cancer Metastases ◽  
Group I ◽  
Long Term Treatment

AbstractIn some breast cancer (BC) patients, an examination of lymph nodes dissected during sentinel lymph node biopsy (SLNB) demonstrates a presence of metastatic lesions and extracapsular extension (ECE) in a SLN. This study aimed to evaluate clinical relevance of ECE in BC patients. This is a retrospective analysis of 891 patients with cancer metastases to SLN, referred to supplementary axillary lymph node dissection (ALND), hospitalized between Jan 2007 and Dec 2017. Clinical and epidemiological data was evaluated. Long-term treatment outcomes were analysed. In 433 (48.6%) patients, cancer metastases were limited to the SLN (group I), in 61 (6.8%) patients the SLN capsule was exceeded focally (≤ 1 mm—group II). In 397 (44.6%) patients, a more extensive ECE was found (> 1 mm—group III). Metastases to non-sentinel lymph nodes (nSLNs) were diagnosed in 27.0% patients from group I, 44.3% patients from group II and in 49.6% patients from group III. No statistically significant differences were observed in long-term treatment outcomes for compared groups. The presence of ECE is accompanied by a higher stage of metastatic lesions in the lymphatic system. The differences in this respect were statistically significant, when compared to the group of ECE(−) patients. ECE, regardless of its extent, did not impact the long-term treatment results. ECE remains an indication for supplementary ALND and for other equivalent cancer treatment procedures, regardless of ECE size.

scholarly journals Long term clinical result of implant induced injury on the adjacent tooth

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yang-Jin Yi ◽  
In-Woo Park ◽  
Jeong-Kui Ku ◽  
Deuk-Won Jo ◽  
Jung-Suk Han ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Root Surface ◽  
Chi Square ◽  
Group Iii ◽  
Direct Invasion ◽  
Adjacent Tooth ◽  
Group I ◽  
Long Term Result ◽  
Root Group

AbstractThe purpose of the retrospective study was to investigate the long-term result of implant-induced injury on the adjacent tooth. The subjects of this retrospective study were patients who had received implants and had tooth injury; direct invasion of root (group I), root surface contact (group II), or < 1 mm distance of the implant from the root (group III). Clinical and pathological changes were periodically examined using radiographs and intra-oral examinations. Paired t-tests and chi-square tests were used to evaluate the implant stability quotient (ISQ) of implant and tooth complications, respectively (α = 0.05). A total of 32 implants and teeth in 28 patients were observed for average 122.7 (± 31.7, minimum 86) months. Seven teeth, three of which were subsequently extracted, needed root canal treatment. Finally, 90.6% of the injured teeth remained functional. Complications were significant and varied according to the group, with group I showing higher events than the others. The ISQs increased significantly. One implant in group I resulted in osseointegration failure. The implant survival rate was 96.9%. In conclusion, it was found even when a tooth is injured by an implant, immediate extraction is unnecessary, and the osseointegration of the invading implant is also predictable.

Analysis of surface conditioning methods on core-veneer bond strength of CAD/CAM zirconia restorations

2020 ◽  
pp. 1-11
Author(s):  
Anip K. Roy ◽  
Govind N. Prasad ◽  
Tushar V. Bhagat ◽  
Saurabh Chaturvedi ◽  
Vishwanath Gurumurthy ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Bond Strength ◽  
Shear Bond Strength ◽  
Group Iv ◽  
Control Group ◽  
Group Iii ◽  
Surface Conditioning ◽  
Group I ◽  
Alumina Particles ◽  
Scanning Electron

BACKGROUND: The increased strength of zirconia has resulted in its widespread application in clinical dentistry. Nevertheless, the fracture of veneering porcelains remains one of the key reasons of failure. OBJECTIVE: The objective of this study was to compare and analyze the influence of surface conditioning methods on the core-veneer bond strength of zirconia restorations. METHODS: Thirty specimens of zirconia core with sizes 10 × 5 × 5 mm were layered with porcelain of sizes 5 × 3 × 3 mm. On the basis of different surface conditioning methods, four groups were made: Group I: abrasion with airborne alumina particles of 110 μm size, Group II: sandblasting with silica coated alumina particles of 50 μm in size, Group III (modified group): alteration with a coating of zirconia powder prior to sintering, and Group IV (control group): metal core specimens. The shear force of all specimens was tested using a universal testing machine with a 0.5 mm/min crosshead speed. One-way analysis of variance (ANOVA) and Tukey’s post hoc pair wise comparison (p= 0.05) were performed to analyze the shear bond strength. A scanning electron microscope was used to assess the fractured specimens. RESULTS: A statistically significant difference was noted between the groups. The mean value of shear bond strength was 40.25 MPa for Group I, 41.93 MPa for Group II, 48.08 MPa for Group III and 47.01 MPa for Group IV. CONCLUSIONS: The modified zirconia group and control group demonstrated a significantly higher mean bond strength than that of Group I, where airborne particle abrasion was used. The scanning electron microscope showed that cohesive fracture in the porcelain veneers was the main problem of failure in altered zirconia. The modified zirconia specimens in Group III demonstrated significantly improved values of shear bond strength.

scholarly journals The Pice Effects of Competition from Parallel Imports and Therapeutic Alternatives: Using Dynamic Models to Estimate the Causal Effect on the Extensive and Intensive Margins

2021 ◽  
Author(s):  
David Granlund
Keyword(s):  
Dynamic Models ◽  
Causal Effect ◽  
The Other ◽  
Causal Effects ◽  
Parallel Imports ◽  
Extensive And Intensive Margins ◽  
Price Effects ◽  
Short And Long Term ◽  
Therapeutic Alternatives

AbstractThis paper studies responses to competition with the use of dynamic models that distinguish between short- and long-term price effects. The dynamic models also allow lagged numbers of competitors to become valid and strong instruments for the current numbers, which enables studying the causal effects using flexible specifications. A first parallel trader is found to decrease prices of exchangeable products by 7% in the long term. On the other hand, prices do not respond to the first competitor that sells therapeutic alternatives; but competition from four or more competitors that sell on-patent therapeutic alternatives decreases prices by about 10% in the long term.

Seasonal variations in wool growth and heat tolerance of sheep. I. Wool growth

1960 ◽  
Vol 11 (1) ◽  
pp. 75 ◽  
Author(s):  
M Wodzicka
Keyword(s):  
Body Weight ◽  
Day Treatment ◽  
The Other ◽  
Seasonal Rhythm ◽  
Group Iii ◽  
Wool Growth ◽  
Group I ◽  
The Mean ◽  
Group Ii ◽  
The Difference

The monthly wool growth of three groups of rams was studied at Beltsville, Maryland. Group I received natural daylight (at 38° 53' N.) and was shorn monthly. Group II had a 7:17 hours of daylight to hours of darkness rhythm and was shorn every 6 months, once in winter and once in summer. Group III received natural daylight and was likewise shorn every 6 months. The rams of all groups produced more wool in summer than in winter. This difference was significant (P<0.001). The mean body weight and food intake were both greater in the winter months, which indicated that the seasonal rhythm of wool growth was not a consequence of poorer feeding in winter. The rams which were shorn monthly (group I) grew considerably more wool than the other two groups, but the difference was not statistically significant. The short-day treatment of group II did not increase the annual wool production nor decrease the seasonal rhythm of wool growth. The balance of evidence from this and other experiments indicates that temperature rather than light controls the seasonal rhythm of wool growth.

scholarly journals Effect and mechanisms of reproductive tract infection on oxidative stress parameters, sperm DNA fragmentation, and semen quality in infertile males

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Kang-Sheng Liu ◽  
Xiao-Dong Mao ◽  
Feng Pan ◽  
Rui Fang An
Keyword(s):  
Reproductive Medicine ◽  
Reproductive Tract ◽  
Group Iv ◽  
Semen Parameters ◽  
Group Iii ◽  
Reproductive Tract Infection ◽  
Group I ◽  
Sperm Dna ◽  
Group Ii ◽  
Elastase Level

AbstractRecent years have seen a rising incidence of male infertility, mostly caused by the decline of sperm quality. The ratio of infertile males to infertile females has escalated from 3:7 in 2013 to current 5:5, which turns male infertility into the research focus of reproductive medicine. This study aimed to clarify the effect of reproductive tract infection by ureaplasma urealyticum (UU) and chlamydia trachomatis (CT) on the DNA integrity and routine semen parameters of infertile males. A retrospective study was performed. A total of 259 infertile males who were treated at the Andrological Laboratory Examination and Reproductive Medicine Center in our hospital were analyzed. qRT-PCR was used to examine the infection status of CT and UU. According to the eligibility criteria, we evaluated the semen parameters and biochemical data of 253 men. Based on the results of PCR, the subjects were divided into four groups: Group I (CT positive, 63 cases), Group II (UU positive, 60 cases), Group III (CT positive and UU positive, 62 cases), and Group IV (no infection, 68 cases). DNA fragmentation index (DFI), sperm count, vitality and morphology, elastase level, seminal plasma malondialdehyde (MDA), and total antioxidant capacity (TAC) were assessed. Compared to Group IV, three groups (Group I, Group II and Group III) showed difference in semen volume, proportion of sperm with normal morphology, sperm motility, progressive motility, and vitality (P < 0.05). Compared to Group IV, Group II and Group III showed difference in DFI (P < 0.05). Compared to Group IV, Group II and Group III showed difference in elastase level (P < 0.05). VCL, VSL, VAP, WOB, ROS, TM, HDS showed differences between groups of abnormal/normal WBC (*P < 0.01).UU infection significantly increased the level of seminal leukocytes only in Group II, but not in the other three groups, indicating that UU is a factor to increase the level of seminal leukocytes. Compared with the normal leukocyte group, there were significant differences in total motility, forward motility and normal sperm ratio between the two groups. The proportion of sperm with abnormal morphology (mostly in the head) showed obvious difference between groups of high and normal seminal leukocytic levels. At the same time, in this study, SCGE and SCD verified that leukocytes could damage sperm DNA by increasing ROS, which ultimately affects male fertility.

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