scholarly journals Inaccuracies associated with the automated measurement of mean cell hemoglobin concentration in dehydrated cells

Blood ◽  
1980 ◽  
Vol 56 (1) ◽  
pp. 125-128 ◽  
Author(s):  
N Mohandas ◽  
MR Clark ◽  
S Kissinger ◽  
C Bayer ◽  
SB Shohet
Keyword(s):  
Light Scattering ◽  
Hemoglobin Concentration ◽  
Red Cell ◽  
Automated Measurement ◽  
Cell Water ◽  
Normal Cells ◽  
Mean Cell Volume ◽  
Water Contents ◽  
Common Laboratory ◽  
Mean Cell Hemoglobin

Abstract Because of discrepancies between electronically and manually measured values of mean cell hemoglobin concentration (MCHC) encountered in studies of pathologic red cells, we studied the effect of cell water content on MCHC measurements by both methods. A series of red cell samples with varying water contents (54%-164% normal) were prepared from normal cells using the antibiotic nystatin. MCHC was then measured, using the microhematocrit centrifuge and three different electronic cell counters in common laboratory use. For MCHC values above 36 g/dl as measured by the spun hematocrit method, all three electronic counters under estimmated the MCHC, with increasing error as the true MCHC increased. For MCHC values below 30 g/dl, the values from two conductivity based instruments agreed with those from the spun hematocrit method, whereas one instrument based on light scattering overestimated the MCHC. These results indicate that inaccuracies in the measured mean cell volume (MCV) of dehydrated or otherwise undeformable cells may lead to spurious values for MCHC when electronic cell counters are used.

scholarly journals Inaccuracies associated with the automated measurement of mean cell hemoglobin concentration in dehydrated cells

Blood ◽  
1980 ◽  
Vol 56 (1) ◽  
pp. 125-128
Author(s):  
N Mohandas ◽  
MR Clark ◽  
S Kissinger ◽  
C Bayer ◽  
SB Shohet
Keyword(s):  
Light Scattering ◽  
Hemoglobin Concentration ◽  
Red Cell ◽  
Automated Measurement ◽  
Cell Water ◽  
Normal Cells ◽  
Mean Cell Volume ◽  
Water Contents ◽  
Common Laboratory ◽  
Mean Cell Hemoglobin

Because of discrepancies between electronically and manually measured values of mean cell hemoglobin concentration (MCHC) encountered in studies of pathologic red cells, we studied the effect of cell water content on MCHC measurements by both methods. A series of red cell samples with varying water contents (54%-164% normal) were prepared from normal cells using the antibiotic nystatin. MCHC was then measured, using the microhematocrit centrifuge and three different electronic cell counters in common laboratory use. For MCHC values above 36 g/dl as measured by the spun hematocrit method, all three electronic counters under estimmated the MCHC, with increasing error as the true MCHC increased. For MCHC values below 30 g/dl, the values from two conductivity based instruments agreed with those from the spun hematocrit method, whereas one instrument based on light scattering overestimated the MCHC. These results indicate that inaccuracies in the measured mean cell volume (MCV) of dehydrated or otherwise undeformable cells may lead to spurious values for MCHC when electronic cell counters are used.

Failure of prolonged exercise training to increase red cell mass in humans

1996 ◽  
Vol 270 (1) ◽  
pp. H121-H126 ◽  
Author(s):  
J. K. Shoemaker ◽  
H. J. Green ◽  
J. Coates ◽  
M. Ali ◽  
S. Grant
Keyword(s):  
Exercise Training ◽  
Prolonged Exercise ◽  
Cell Mass ◽  
Hemoglobin Concentration ◽  
Red Cell ◽  
Total Blood ◽  
Mean Cell Volume ◽  
Red Cell Mass ◽  
Mean Cell Hemoglobin

The purpose of this study was to investigate the time-dependent effects of long-term prolonged exercise training on vascular volumes and hematological status. Training using seven untrained males [age 21.1 +/- 1.4 (SE) yr] initially consisted of cycling at 68% of peak aerobic power (VO2peak) for 2 h/day, 4-5 days/wk, for 11 wk. Absolute training intensity was increased every 3 wk. Red cell mass (RCM), obtained using 51Cr, was unchanged (P > 0.05) with training (2,142 +/- 95, 2,168 +/- 86, 2,003 +/- 112, and 2,080 +/- 116 ml at 0, 3, 6, and 11 wk, respectively) as were serum erythropoietin levels (17.1 +/- 4.3, 13.9 +/- 3.5, and 17.0 +/- 2.0 U/l at 0, 6, and 11 wk, respectively). Plasma volume measured with 125I-labeled albumin and total blood volume (TBV) were also not significantly altered. The increase in mean cell volume that occurred with training (89.7 +/- 0.95 vs. 91.0 +/- 1.0 fl, 0 vs. 6 wk, P < 0.05) was not accompanied by changes in either mean cell hemoglobin or mean cell hemoglobin concentration. Serum ferritin was reduced 73% with training (67.4 +/- 13 to 17.9 +/- 1 microgram/l, 0 vs. 11 wk, P < 0.05). Total hemoglobin (HbTot) calculated as the product of hemoglobin concentration and TBV was unaltered (P > 0.05) at both 6 and 11 wk of training. The 15% increase in VO2peak (3.39 +/- 0.16 to 3.87 +/- 0.14 l/min, 0 vs. 11 wk, P < 0.05) with training occurred despite a failure of training to change TBV, RCM, or HbTot.

scholarly journals Influence of red cell water content on the morphology of sickling

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 823-830 ◽  
Author(s):  
MR Clark ◽  
JC Guatelli ◽  
N Mohandas ◽  
SB Shohet
Keyword(s):  
Water Content ◽  
Hemoglobin Concentration ◽  
Major Influence ◽  
Red Cell ◽  
Hypotonic Medium ◽  
Cell Water ◽  
Sickle Cells ◽  
Membrane Rigidity ◽  
Internal Viscosity ◽  
Mean Cell Hemoglobin

Abstract The response of sickle cells with varying water content to alterations in oxygen tension has been studied. Cells that were severely dehydrated while sickled retained the characteristic sickled morphology even after prolonged reoxygenation. When the cell water content was increased by reduction of the suspending medium osmolality, the cells unsickled. Cells that were dehydrated before deoxygenation were unable to assume the spiculated morphology typical of sicked cells. This was true both for high mean cell hemoglobin concentration (MCHC) discoid sickle cells and for irreversibly sickled cells. When such cells were resuspended in hypotonic medium before deoxygenation, they sickled with the characteristic morphology of sickle cells with normal MCHC. The morphological behavior of Ca-loaded sickled cells as well as irreversibly sickled cells showed a major influence of increased hemoglobin concentration and extremely high internal viscosity. Constraint on cell morphology by putative membrane rigidity was not observed.

scholarly journals Influence of red cell water content on the morphology of sickling

Blood ◽  
1980 ◽  
Vol 55 (5) ◽  
pp. 823-830 ◽  
Author(s):  
MR Clark ◽  
JC Guatelli ◽  
N Mohandas ◽  
SB Shohet
Keyword(s):  
Water Content ◽  
Hemoglobin Concentration ◽  
Major Influence ◽  
Red Cell ◽  
Hypotonic Medium ◽  
Cell Water ◽  
Sickle Cells ◽  
Membrane Rigidity ◽  
Internal Viscosity ◽  
Mean Cell Hemoglobin

The response of sickle cells with varying water content to alterations in oxygen tension has been studied. Cells that were severely dehydrated while sickled retained the characteristic sickled morphology even after prolonged reoxygenation. When the cell water content was increased by reduction of the suspending medium osmolality, the cells unsickled. Cells that were dehydrated before deoxygenation were unable to assume the spiculated morphology typical of sicked cells. This was true both for high mean cell hemoglobin concentration (MCHC) discoid sickle cells and for irreversibly sickled cells. When such cells were resuspended in hypotonic medium before deoxygenation, they sickled with the characteristic morphology of sickle cells with normal MCHC. The morphological behavior of Ca-loaded sickled cells as well as irreversibly sickled cells showed a major influence of increased hemoglobin concentration and extremely high internal viscosity. Constraint on cell morphology by putative membrane rigidity was not observed.

scholarly journals Dynamic changes in murine erythropoiesis from birth to adulthood: implications for the study of murine models of anemia

2020 ◽  
Vol 5 (1) ◽  
pp. 16-25
Author(s):  
Lixiang Chen ◽  
Jie Wang ◽  
Jing Liu ◽  
Hua Wang ◽  
Christopher D. Hillyer ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Steady State ◽  
Hemoglobin Concentration ◽  
Red Cell ◽  
Murine Models ◽  
Erythropoietic Activity ◽  
Dynamic Changes ◽  
Newborn Mice ◽  
Mean Cell Volume ◽  
Cell Parameters

Abstract Liver, spleen, and bone marrow are 3 key erythropoietic tissues in mammals. In the mouse, the liver is the predominant site of erythropoiesis during fetal development, the spleen responds to stress erythropoiesis, and the bone marrow is involved in maintaining homeostatic erythropoiesis in adults. However, the dynamic changes and respective contributions of the erythropoietic activity of these tissues from birth to adulthood are incompletely defined. Using C57BL/6 mice, we systematically examined the age-dependent changes in liver, spleen, and bone marrow erythropoiesis following birth. In addition to bone marrow, the liver and spleen of newborn mice sustain an active erythropoietic activity that is gradually lost during first few weeks of life. While the erythropoietic activity of the liver is lost 1 week after birth, that of the spleen is maintained for 7 weeks until the erythropoietic activity of the bone marrow is sufficient to sustain steady-state adult erythropoiesis. Measurement of the red cell parameters demonstrates that these postnatal dynamic changes are reflected by varying indices of circulating red cells. While the red cell numbers, hemoglobin concentration, and hematocrit progressively increase after birth and reach steady-state levels by week 7, reticulocyte counts decrease during this time period. Mean cell volume and mean cell hemoglobin progressively decrease and reach steady state by week 3. Our findings provide comprehensive insights into developmental changes of murine erythropoiesis postnatally and have significant implications for the appropriate interpretation of findings from the variety of murine models used in the study of normal and disordered erythropoiesis.

Chronic sodium cyanate treatment induces "hypoxia-like" effects in rats

1986 ◽  
Vol 60 (4) ◽  
pp. 1145-1149 ◽  
Author(s):  
B. P. Teisseire ◽  
C. C. Vieilledent ◽  
L. J. Teisseire ◽  
M. O. Vallez ◽  
R. A. Herigault ◽  
...  
Keyword(s):  
Right Ventricular ◽  
Pressor Response ◽  
Acute Hypoxia ◽  
Hemoglobin Concentration ◽  
Normal Growth ◽  
Mean Cell Volume ◽  
Control Value ◽  
Normal Limits ◽  
Sodium Cyanate ◽  
Mean Cell Hemoglobin

Three weeks of sodium cyanate (NaCNO) intraperitoneal treatment in rats (n = 15) induced high hemoglobin O2 affinity, i.e., low PO2 at 50% hemoglobin saturation (P50), 20.5 +/- 1.4 Torr, in comparison with the mean control values, 34.5 +/- 1.6 Torr (n = 15). NaCNO rats showed a reduction in mean body weight, 376 +/- 27 g, in comparison with controls, 423 +/- 23 g (P less than 0.001). Despite arterial O2 partial pressure (PaO2) within normal limits NaCNO-treated rats had a higher systolic right ventricular pressure (SRVP), 33.7 +/- 3.1 Torr, in comparison with control value, 29.0 +/- 2.5 Torr (P less than 0.001). Right ventricle weights were significantly increased (P less than 0.001). After 60 min of an hypoxic challenge (fractional concentration of inspired O2 = 0.10) NaCNO-treated rats increased SRVP of only 7 +/- 4% compared with 46 +/- 9% in the control animals. Inducing high hemoglobin affinity in rats (n = 10; 6 wk NaCNO treatment) resulted in increases in hematocrit ratio and hemoglobin concentration (P less than 0.001). The characteristics of the red blood cell (RBC) itself changed; values of mean cell volume, mean cell hemoglobin, and mean cell hemoglobin concentration being significantly increased (P less than 0.001) when compared with mean control values. The count of nucleated RBC′s appeared to be significantly higher from the 2nd wk of NaCNO treatment. Chronic NaCNO treatment was demonstrated to exert “hypoxia-like” effects since it induced prevention of normal growth, polycythemia, pulmonary hypertension, right ventricular hypertrophy, and blunted pulmonary pressor response to acute hypoxia.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Marked interference of hyperglycemia in measurements of mean (red) cell volume by Technicon H analyzers

1996 ◽  
Vol 42 (1) ◽  
pp. 76-80 ◽  
Author(s):  
H L van Duijnhoven ◽  
M Treskes
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Cell Volume ◽  
Sodium Dodecyl ◽  
Hemoglobin Concentration ◽  
Red Cell ◽  
Erythrocyte Volume ◽  
Red Cell Volume ◽  
Erythrocyte Indices ◽  
Dodecyl Sulfate ◽  
Mean Cell Hemoglobin

Abstract Severe hyperglycemia can result in falsely high results for mean cell (erythrocyte) volume (MCV), which will also cause false results for erythrocyte indices calculated on the basis of MCV. Falsely high MCV results were obtained with the Technicon H1 and H2 analyzers and (to a lesser extent) with the Coulter T660. The H analyzers were more susceptible to this interference than was the Coulter T660. This difference in sensitivity of MCV to hyperglycemia can be explained by the use of sodium dodecyl sulfate in the Technicon erythrocyte diluent and by differences in incubation times. In severe hyperglycemia, results for MCV, mean cell hemoglobin concentration, and hematocrit obtained from electronic cell counters, especially Technicon H systems, are unreliable.

scholarly journals The hematological parameters in clinically normal Iraqi local breed goats

2015 ◽  
Vol 39 (2) ◽  
pp. 12-16
Author(s):  
AH. H. AL-Hadithy
Keyword(s):  
Blood Cell ◽  
Cell Volume ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Mean Cell Volume ◽  
Local Breed ◽  
Clinically Normal ◽  
Significant Difference ◽  
Males And Females ◽  
Mean Cell Hemoglobin

     The aim of the present work is to determine the hematological parameters in clinically normal local breed goats. The study was conducted on 130 goats (40 males of them 20 bucks and 20 buck kids and 90 females of them 20 doe kids, 20 dry does, 25 pregnant and 25 lactating does) aged 6 months-4 years in AL-Najaf province-Iraq. Blood samples were collected from the jugular vein into EDTA tubes during October 2013 untill February 2014. Results showed that the ranges and means ±SE of blood picture were as follows; packed cell volume (PCV) 26-40% and 31.8±0.32%, Hemoglobin (Hb) 8-14.3g/dL and 10.3±0.13 g/dL, Red blood cell (RBC) 10.6-17×106/μL and 13.3±0.14×106/μL, Mean cell volume (MCV) 18.4-32.5 fL and 23.9±0.14 fL, Mean cell Hemoglobin (MCH) 5.5-8.8 and 7.7±0.05 pg, Mean cell hemoglobin concentration (MCHC) 28.3-36.9 g/dL and  32.4±0.15 g/dL, Erythrocyte sedimentation (ESR) 1-10 mm/ 24h and 4.1±0.14 mm/ 24h, White blood cell count (WBC) 5500-15900/μL and 10732±242/μL, Lymphocyte (L) 2772-10172/μL and 6080±149 /Μl, neutrophils (N) 1863-8680/μL and 4112±121/μL, monocytes 57.0-622/μL and313±11/μL, eosinophil’s 0-918/μL and 222±14/μL, basophils 0±0/μL and 0±0/μL, L/N ratio 0.4-3.8 and 1.5±0.04, respectively. There was a significant (P<0.05) increase in PCV, Hb, RBC, MCV, MCH, MCHC and no difference in ESR,WBC, DLC and L/N ratio between males and females. However, significant (P<0.05) differences in most hematological parameters of sub groups have been recorded. In conclusion the present data recorded the reference values of hematological parameters in clinically healthy local breed goats with a significant difference between males and females as well as in normal subgroups.

scholarly journals Accurate and independent measurement of volume and hemoglobin concentration of individual red cells by laser light scattering

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 506-513 ◽  
Author(s):  
N Mohandas ◽  
YR Kim ◽  
DH Tycko ◽  
J Orlik ◽  
J Wyatt ◽  
...  
Keyword(s):  
Light Scattering ◽  
Cell Volume ◽  
Concentration Distribution ◽  
Laser Light ◽  
Hemoglobin Concentration ◽  
Laser Light Scattering ◽  
Red Cells ◽  
Red Cell ◽  
Flow Cytometric ◽  
Wide Range

Cell volume (MCV) and hemoglobin concentration (MCHC) are the red cell indices used to characterize the blood of patients with anemia. Since the introduction of flow cytometric methods for the measurement of these indices, it has generally been assumed that the values derived by these instruments are accurate. However, it has recently been shown that a number of cellular factors, including alterations in cellular deformability, can lead to inaccurate measurement of cell volume by these automated instruments. Because cell hemoglobin concentration and hematocrit are computed from the measured values of cell volume, accuracy of these indices is also compromised by inaccurate determination of cell volume. A recently developed experimental flow cytometric method based on laser light scattering, which can independently measure volume and hemoglobin concentration, has been used in the present study to measure MCV and MCHC of density- fractionated normal and sickle red cells, hydrated and dehydrated normal red cells, and various pathologic cells. We found that the new method accurately measures both volume and hemoglobin concentrations over a wide range of MCV (30 to 120 fL) and MCHC (27 to 45 g/dL) values. This is in contrast to currently available methods in which hemoglobin concentration values are accurately measured over a more limited range (27 to 35 g/dL). In addition, as the experimental method independently measures volume and hemoglobin concentration of individual red cells, it allowed us to generate histograms of volume and hemoglobin concentration distribution and derive coefficient of variation for volume distribution and standard deviation of hemoglobin concentration distribution. We have been able to document that volume and hemoglobin concentration distributions can vary independently of each other in pathologic red cell samples.

Modulation of Hematological Indices of Normal and Alloxan-Induced Diabetic Rabbits by Aqueous Extract of Pleurotus tuberregium Sclerotia

2020 ◽  
Vol 20 (3) ◽  
pp. 380-387
Author(s):  
Ifeanacho M. Onuekwuzu ◽  
Ikewuchi C. Chidinma ◽  
Ikewuchi J. Chigozie
Keyword(s):  
Aqueous Extract ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Diabetic Control ◽  
Red Cell ◽  
Distribution Width ◽  
Hematological Indices ◽  
Diabetic Rabbits ◽  
Pleurotus Tuberregium ◽  
Mean Cell Hemoglobin

Objective:: The ability of an aqueous extract of the sclerotia of Pleurotus tuberregium to modulate hematological parameters was investigated in normal and alloxan treated rabbits. Methods:: The extract was subjected to atomic absorption spectrophotometric and flame ionization detector-coupled-gas chromatographic (GC-FID) analysis. Diabetes mellitus was induced by a 120 mg/kg body weight intravenous injection of alloxan. Metformin was orally administered at 50 mg/kg, while the extract was administered (both to normal and diabetic rabbits) at 100, 200 and 300 mg/kg. Results:: Analysis of the extract showed that it had high contents of calcium, magnesium, manganese and potassium. Eleven known glycosides were detected, comprising mainly of amygdalin (37.7%), digoxin (14.4%), dhurrin (14.0%), linamarin (13.6%), prunasin (10.8%) and digitoxin (8.4%). Also detected were twelve known saponins, consisting mainly of sapogenin (40.3%) and neochlorogenin (21.8%); and twelve known lignans, consisting mainly of matairesinol (59.7%), secoisolariciresinol (20.9%) and lariciresinol (14.9%). Compared to the Diabetic control, the hematocrit, hemoglobin concentration, mean cell hemoglobin, mean cell hemoglobin concentration, mean corpuscular volume, red cell distribution width; and red cell, total white cell, lymphocytes, granulocytes and platelet counts of the treated groups were significantly (p<0.05) higher. Conclusion:: The above result showed that the extract had a positive effect on the hemopoietic system of the treated animals, at least at the doses at which it was administered in this study.

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