Chronic sodium cyanate treatment induces "hypoxia-like" effects in rats

Three weeks of sodium cyanate (NaCNO) intraperitoneal treatment in rats (n = 15) induced high hemoglobin O2 affinity, i.e., low PO2 at 50% hemoglobin saturation (P50), 20.5 +/- 1.4 Torr, in comparison with the mean control values, 34.5 +/- 1.6 Torr (n = 15). NaCNO rats showed a reduction in mean body weight, 376 +/- 27 g, in comparison with controls, 423 +/- 23 g (P less than 0.001). Despite arterial O2 partial pressure (PaO2) within normal limits NaCNO-treated rats had a higher systolic right ventricular pressure (SRVP), 33.7 +/- 3.1 Torr, in comparison with control value, 29.0 +/- 2.5 Torr (P less than 0.001). Right ventricle weights were significantly increased (P less than 0.001). After 60 min of an hypoxic challenge (fractional concentration of inspired O2 = 0.10) NaCNO-treated rats increased SRVP of only 7 +/- 4% compared with 46 +/- 9% in the control animals. Inducing high hemoglobin affinity in rats (n = 10; 6 wk NaCNO treatment) resulted in increases in hematocrit ratio and hemoglobin concentration (P less than 0.001). The characteristics of the red blood cell (RBC) itself changed; values of mean cell volume, mean cell hemoglobin, and mean cell hemoglobin concentration being significantly increased (P less than 0.001) when compared with mean control values. The count of nucleated RBC′s appeared to be significantly higher from the 2nd wk of NaCNO treatment. Chronic NaCNO treatment was demonstrated to exert “hypoxia-like” effects since it induced prevention of normal growth, polycythemia, pulmonary hypertension, right ventricular hypertrophy, and blunted pulmonary pressor response to acute hypoxia.(ABSTRACT TRUNCATED AT 250 WORDS)

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Inflammatory cytokine inhibition of erythropoiesis in patients implanted with a mechanical circulatory assist device

Perfusion ◽

10.1191/0267659105pf793oa ◽

2005 ◽

Vol 20 (2) ◽

pp. 83-90 ◽

Cited By ~ 136

Author(s):

Christopher N Pierce ◽

Douglas F Larson

Keyword(s):

Inflammatory Cytokine ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Mean Cell Volume ◽

Distribution Width ◽

Normal Limits ◽

Circulatory Assist Devices ◽

Cytokine Inhibition ◽

Normal Measure ◽

The University

Mechanical circulatory assist devices (MCADs) are increasingly utilized independently of cardiac transplantation in the management of heart failure. Though MCAD use incorporates inherent mechanical risks, the inevitable onset of chronic anemia, with its associated morbidity and mortality, is also a significant concern. MCAD support has been correlated with elevated plasma levels of inflammatory cytokines TNF-α, IL-1b, and IL-6, which have separately been found to inhibit erythropoietin (Epo)-induced erythrocyte (RBC) maturation. Previous analysis of hematological parameters for MCAD-sup-ported patients concluded that an amplified inflammatory response impedes RBC proliferation and recovery from hemolytic anemia. Additional analysis may bolster this assertion. Hemoglobin concentration (HC), RBC distribution width (RDW), mean cell volume (MCV), and cardiac index were retrospectively analysed for 78 MCAD-supported patients implanted for greater than 30 days at the University of Arizona Health Sciences Center from 1996 to 2002. Analysis confirms that the HC, a conventional marker for anemia, declines with MCAD placement and remains below the clinically defined, minimum normal value. Inversely, the RDW rises above maximum normal measure, signifying an increased fraction of juvenile RBCs. The MCV remains unchanged and within normal limits, demonstrating adequate substrate for RBC formation. MCAD performance also stabilizes as adequate perfusion returns. These results further support our previously published conclusion that a sufficient response of erythropoiesis occurs in reaction to the onset of anemia by an increased production of immature RBCs. However, the cells never fully mature and join circulation. The patient’s inflammatory cytokine response to the implanted device most likely mediates the chronic MCAD-induced anemia by inhibition of Epo effects.

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Failure of prolonged exercise training to increase red cell mass in humans

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1996.270.1.h121 ◽

1996 ◽

Vol 270 (1) ◽

pp. H121-H126 ◽

Cited By ~ 4

Author(s):

J. K. Shoemaker ◽

H. J. Green ◽

J. Coates ◽

M. Ali ◽

S. Grant

Keyword(s):

Exercise Training ◽

Prolonged Exercise ◽

Cell Mass ◽

Hemoglobin Concentration ◽

Red Cell ◽

Total Blood ◽

Mean Cell Volume ◽

Red Cell Mass ◽

Mean Cell Hemoglobin

The purpose of this study was to investigate the time-dependent effects of long-term prolonged exercise training on vascular volumes and hematological status. Training using seven untrained males [age 21.1 +/- 1.4 (SE) yr] initially consisted of cycling at 68% of peak aerobic power (VO2peak) for 2 h/day, 4-5 days/wk, for 11 wk. Absolute training intensity was increased every 3 wk. Red cell mass (RCM), obtained using 51Cr, was unchanged (P > 0.05) with training (2,142 +/- 95, 2,168 +/- 86, 2,003 +/- 112, and 2,080 +/- 116 ml at 0, 3, 6, and 11 wk, respectively) as were serum erythropoietin levels (17.1 +/- 4.3, 13.9 +/- 3.5, and 17.0 +/- 2.0 U/l at 0, 6, and 11 wk, respectively). Plasma volume measured with 125I-labeled albumin and total blood volume (TBV) were also not significantly altered. The increase in mean cell volume that occurred with training (89.7 +/- 0.95 vs. 91.0 +/- 1.0 fl, 0 vs. 6 wk, P < 0.05) was not accompanied by changes in either mean cell hemoglobin or mean cell hemoglobin concentration. Serum ferritin was reduced 73% with training (67.4 +/- 13 to 17.9 +/- 1 microgram/l, 0 vs. 11 wk, P < 0.05). Total hemoglobin (HbTot) calculated as the product of hemoglobin concentration and TBV was unaltered (P > 0.05) at both 6 and 11 wk of training. The 15% increase in VO2peak (3.39 +/- 0.16 to 3.87 +/- 0.14 l/min, 0 vs. 11 wk, P < 0.05) with training occurred despite a failure of training to change TBV, RCM, or HbTot.

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Profil Darah Ikan Gelodok (Periophthalmodon schlosseri) dan (Boleophthalamus boddarti) di Desa Kuala Tambangan Pelaihari, Kalimantan Selatan

Jurnal Pharmascience ◽

10.20527/jps.v7i2.8465 ◽

2020 ◽

Vol 7 (2) ◽

pp. 176

Author(s):

Nita Azhari ◽

Hidayaturrahmah Hidayaturrahmah

Keyword(s):

Normal Limit ◽

Hemoglobin Concentration ◽

Mangrove Ecosystem ◽

Normal Range ◽

Blood Profile ◽

Normal Limits ◽

Range Type ◽

Periophthalmodon Schlosseri ◽

The Village ◽

Mean Cell Hemoglobin

Profil darah memiliki peran yang sangat penting dalam fisiologi metabolisme dan aktifitas tubuh hewan. Kuala Tambangan memiliki banyak potensi sumber daya ikan salah satunya ikan gelodok. Ikan gelodok di kawasan ini mudah ditemukan, akan tetapi sampai sekarang belum dimanfaatkan dengan baik oleh masyarakat. Tujuan penelitian ini untuk mengetahui profil darah ikan gelodok jenis Periophthalmodon schlosseri dan Boleophthalamus boddarti pada ekosistem mangrove yang berada di desa Kuala Tambangan, Kabupaten Tanah Laut, Kalimantan Selatan. Metode yang digunakan pada penelitian ini yaitu metode penangkapan hewan langka yaitu line transek, metode hemositometer dan metode sahli parameter yang dihitung hemoglobin, eritrosit, leukosit, hematokrit, MCV (Mean Corpusculla Volume), MCH (Mean Cell Hemoglobin), MCHC (Mean Cell Hemoglobin Concentration) pada 2 jenis ikan gelodok 34 ekor P. schlosseri dan 34 ekor B. boddarti. Hasil yang didapatkan adalah eritrosit P. schlosseri 3,87±0,58 x 106 sel/μL; B. boddarti 3,78±0,73 x 106 sel/μL 2 jenis ikan gelodok ini memiliki nilai eritrosit diatas batas normal; leukosit P. schlosseri 11,91±5,61 x103 sel/μL dan B. boddartii 9,72±4,24 x103 sel/μL nilai leukosit pada 2 jenis ikan gelodok ini berada di atas batas normal; hemoglobin P. schlosseri 11,59±1,75 % dan B. boddartii 11,75±1,96 % dari hasil yang didapat kadar hemoglobin pada 2 jenis ikan gelodok ini berada di atas batas normal kadar hemoglobin ikan pada umumnya; hematokrit P. schlosseri 34,32±5,57 % dan B. boddartii 35,71±5,44 % hasil hematokrit yang didapat dari 2 jenis ikan gelodok ini yaitu di atas batas normal; jenis P. schlosseri memiliki nilai MCV 88,72±6,62 μm3 yang berada di bawah batas normal; MCH 29,92±0,69 pg/sel yang berada di bawah batas normal; MCHC 33,99±3,97 g/dL pada jenis ini nilai MCHC masih berada pada batas normal; jenis B. boddartii memiliki nilai MCV 96,16±17,96 μm3 yang berada di bawah batas normal; MCH 31,51±5,50 pg/sel yang masih berada di batas normal; MCHC 32,87±1,77 g/dL nilai MCHC pada jenis ini masih berada pada batas normal. Blood profile has a very important role in the physiology of metabolism and animal body activities. Kuala Tambangan has a lot of potential fish resources, one of which is the fish Mudskipper. Mudskipper fish in this area are easy to find, but until now it has not been utilized properly by the community. The purpose of this study was to determine the blood profile of the Periophthalmodon Schlosseri and Boleophthalamus boddarti fish species in the mangrove ecosystem in the village of Kuala Tambangan, Tanah Laut District, South Kalimantan . The method used in this study is the method of catching endangered animals namely trasnek line, hemocytometer method and parameter Sahli method which is calculated hemoglobin, erythrocytes, leukocytes, hematocrit, MCV (Mean Corpuscular Volume), MCH (Mean Cell Hemoglobin), MCHC (Mean Cell Hemoglobin Concentration) on 2 types of fish, 34 Periophthalmodon schlosseri and 34 Boleophthalmus boddarti. The results obtained were P. schlosseri erythrocytes 3.87 ± 0.58 x 106 cells / μL; B. boddarti 3.78 ± 0.73 x 106 cells / μL 2 types of fish Mudskipper has erythrocyte values above the normal limit; P. schlosseri leukocytes 11.91 ± 5.61 x103 cells / μL and B. boddartii 9.72 ± 4.24 x103 cells / μL leukocyte values in these 2 types of jagged fish are above normal limits; hemoglobin P. schlosseri 11.59 ± 1.75 % and B. boddartii 11.75 ± 1.96 % from the results obtained by the hemoglobin levels in these 2 types of fishes are above the normal limit of fish hemoglobin levels in general; hematocrit P.schlosseri 34.32 ± 5.57 % and B. boddartii 35.71 ± 5.44 % hematocrit results obtained from these 2 types of mudskipper fish are above normal limits; P.schlosseri species had MCV values of 88.72 ± 6.62 μm3 which were below normal limits; MCH 29.92 ± 0.69 pg / cell that is below the normal range; MCHC 33.99 ± 3.97 g / dL in this type the MCHC value is still in the normal range; type B. boddartii has a MCV value of 96.16 ± 17.96 μm3 which is below normal limits; MCH 31,51 ± 5.50 pg / cell which is still in the normal range; MCHC 32.87 ± 1.77 g / dL MCHC values in this type are still within normal limits.Keywords: gelodok, blood, kuala tambangan

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The hematological parameters in clinically normal Iraqi local breed goats

The Iraqi Journal of Veterinary Medicine ◽

10.30539/iraqijvm.v39i2.171 ◽

2015 ◽

Vol 39 (2) ◽

pp. 12-16

Author(s):

AH. H. AL-Hadithy

Keyword(s):

Blood Cell ◽

Cell Volume ◽

Hematological Parameters ◽

Hemoglobin Concentration ◽

Mean Cell Volume ◽

Local Breed ◽

Clinically Normal ◽

Significant Difference ◽

Males And Females ◽

Mean Cell Hemoglobin

The aim of the present work is to determine the hematological parameters in clinically normal local breed goats. The study was conducted on 130 goats (40 males of them 20 bucks and 20 buck kids and 90 females of them 20 doe kids, 20 dry does, 25 pregnant and 25 lactating does) aged 6 months-4 years in AL-Najaf province-Iraq. Blood samples were collected from the jugular vein into EDTA tubes during October 2013 untill February 2014. Results showed that the ranges and means ±SE of blood picture were as follows; packed cell volume (PCV) 26-40% and 31.8±0.32%, Hemoglobin (Hb) 8-14.3g/dL and 10.3±0.13 g/dL, Red blood cell (RBC) 10.6-17×106/μL and 13.3±0.14×106/μL, Mean cell volume (MCV) 18.4-32.5 fL and 23.9±0.14 fL, Mean cell Hemoglobin (MCH) 5.5-8.8 and 7.7±0.05 pg, Mean cell hemoglobin concentration (MCHC) 28.3-36.9 g/dL and 32.4±0.15 g/dL, Erythrocyte sedimentation (ESR) 1-10 mm/ 24h and 4.1±0.14 mm/ 24h, White blood cell count (WBC) 5500-15900/μL and 10732±242/μL, Lymphocyte (L) 2772-10172/μL and 6080±149 /Μl, neutrophils (N) 1863-8680/μL and 4112±121/μL, monocytes 57.0-622/μL and313±11/μL, eosinophil’s 0-918/μL and 222±14/μL, basophils 0±0/μL and 0±0/μL, L/N ratio 0.4-3.8 and 1.5±0.04, respectively. There was a significant (P<0.05) increase in PCV, Hb, RBC, MCV, MCH, MCHC and no difference in ESR,WBC, DLC and L/N ratio between males and females. However, significant (P<0.05) differences in most hematological parameters of sub groups have been recorded. In conclusion the present data recorded the reference values of hematological parameters in clinically healthy local breed goats with a significant difference between males and females as well as in normal subgroups.

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Chronic propranolol treatment blunts right ventricular hypertrophy in rats at high altitude

Journal of Applied Physiology ◽

10.1152/jappl.1980.48.3.473 ◽

1980 ◽

Vol 48 (3) ◽

pp. 473-478 ◽

Cited By ~ 15

Author(s):

N. F. Voelkel ◽

I. F. McMurtry ◽

J. T. Reeves

Keyword(s):

Right Ventricular ◽

High Altitude ◽

Ventricular Hypertrophy ◽

Pressor Response ◽

Acute Hypoxia ◽

Right Ventricular Hypertrophy ◽

Receptor Blockade ◽

Beta Receptor ◽

Propranolol Treatment ◽

Beta Receptor Blockade

Chronic beta-receptor blockade has been reported to inhibit right ventricular hypertrophy in rats at high altitude. If so, we wanted to determine whether beta-receptor blockade or some other drug action were involved and whether the heart, the lung vessels, or blood alterations were affected. In rats, chronic treatment with DL-propranolol (2 mg/kg ip once daily) reduced right ventricular hypertrophy and polycythemia of chronic high altitude. D-Propranolol and metoprolol did not reduce hypoxia-induced right ventricular hypertrophy or polycythemia. In isolated lungs from low-altitude rats treated chronically with DL-propranolol or with D-propranolol the pressor response to acute hypoxia was blunted. Chronic DL-propranolol blunted the acute hypoxic pressor response and angiotensin II induced vasoconstriction in lungs from high-altitude rats. Two effects of DL-propranolol treatment were seen: 1) blockade of beta 2-adrenergic receptors, which reduced the right ventricular hypertrophy of high altitude through reduction of hematocrit; and 2) a non-beta-effect, which reduced vascular responsiveness to acute hypoxia in the isolated lung preparation.

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Inaccuracies associated with the automated measurement of mean cell hemoglobin concentration in dehydrated cells

Blood ◽

10.1182/blood.v56.1.125.125 ◽

1980 ◽

Vol 56 (1) ◽

pp. 125-128 ◽

Cited By ~ 30

Author(s):

N Mohandas ◽

MR Clark ◽

S Kissinger ◽

C Bayer ◽

SB Shohet

Keyword(s):

Light Scattering ◽

Hemoglobin Concentration ◽

Red Cell ◽

Automated Measurement ◽

Cell Water ◽

Normal Cells ◽

Mean Cell Volume ◽

Water Contents ◽

Common Laboratory ◽

Mean Cell Hemoglobin

Abstract Because of discrepancies between electronically and manually measured values of mean cell hemoglobin concentration (MCHC) encountered in studies of pathologic red cells, we studied the effect of cell water content on MCHC measurements by both methods. A series of red cell samples with varying water contents (54%-164% normal) were prepared from normal cells using the antibiotic nystatin. MCHC was then measured, using the microhematocrit centrifuge and three different electronic cell counters in common laboratory use. For MCHC values above 36 g/dl as measured by the spun hematocrit method, all three electronic counters under estimmated the MCHC, with increasing error as the true MCHC increased. For MCHC values below 30 g/dl, the values from two conductivity based instruments agreed with those from the spun hematocrit method, whereas one instrument based on light scattering overestimated the MCHC. These results indicate that inaccuracies in the measured mean cell volume (MCV) of dehydrated or otherwise undeformable cells may lead to spurious values for MCHC when electronic cell counters are used.

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Evaluation and additional recommendations for preparing a whole blood control material

Revista de Saúde Pública ◽

10.1590/s0034-89101998000200001 ◽

1998 ◽

Vol 32 (2) ◽

pp. 107-111

Author(s):

Nilda E. Fink ◽

Alejandra Fernández Alberti ◽

Isabel Crispini ◽

Norberto V. Cabutti ◽

Daniel Mazziotta

Keyword(s):

Coefficient Of Variation ◽

Whole Blood ◽

Blood Cells ◽

Low Cost ◽

White Blood Cells ◽

Hemoglobin Concentration ◽

Control Material ◽

Mean Cell Volume ◽

Automated Methods ◽

Mean Cell Hemoglobin

OBJECTIVE: The assessment of an easy to prepare and low cost control material for Hematology, available for manual and automated methods. MATERIAL AND METHOD: Aliquots of stabilized whole blood were prepared by partial fixation with aldehydes; the stability at different temperatures (4. 20 and 37 °C) during periods of up to 8-9 weeks and aliquot variability with both methods were controlled. RESULTS: Aliquot variability with automated methods at day 1, expressed as CV% (coefficient of variation) was: white blood cells (WBC) 2.7, red blood cells (RBC) 0.7, hemoglobin (Hb) 0.6, hematocrit (Hct) 0.7, mean cell volume (MCV) 0.3, mean cell hemoglobin (MCH) 0.6, mean cell hemoglobin concentration (MCHC) 0.7, and platelets (PLT) 4.6. The CV (coefficient of variation) percentages obtained with manual methods in one of the batches were: WBC 23, Hct 2.8, Hb 4.5, MCHC 5.9, PLT 41. Samples stored at 4ºC and 20ºC showed good stability, only a very low initial hemolysis being observed, whereas those stored at 37ºC deteriobed a rapidly (metahemoglobin formation, aggregation of WBC and platelets, as well as alteration of erythrocyte indexes). CONCLUSIONS: It was confirmed that, as long as there is no exposure to high temperatures during distribution, this material is stable, allowing assessment, both esternal and internal, for control purposes, with acceptable reproductivity, both for manual and auttomatic methods.

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Inaccuracies associated with the automated measurement of mean cell hemoglobin concentration in dehydrated cells

Blood ◽

10.1182/blood.v56.1.125.bloodjournal561125 ◽

1980 ◽

Vol 56 (1) ◽

pp. 125-128

Author(s):

N Mohandas ◽

MR Clark ◽

S Kissinger ◽

C Bayer ◽

SB Shohet

Keyword(s):

Light Scattering ◽

Hemoglobin Concentration ◽

Red Cell ◽

Automated Measurement ◽

Cell Water ◽

Normal Cells ◽

Mean Cell Volume ◽

Water Contents ◽

Common Laboratory ◽

Mean Cell Hemoglobin

Because of discrepancies between electronically and manually measured values of mean cell hemoglobin concentration (MCHC) encountered in studies of pathologic red cells, we studied the effect of cell water content on MCHC measurements by both methods. A series of red cell samples with varying water contents (54%-164% normal) were prepared from normal cells using the antibiotic nystatin. MCHC was then measured, using the microhematocrit centrifuge and three different electronic cell counters in common laboratory use. For MCHC values above 36 g/dl as measured by the spun hematocrit method, all three electronic counters under estimmated the MCHC, with increasing error as the true MCHC increased. For MCHC values below 30 g/dl, the values from two conductivity based instruments agreed with those from the spun hematocrit method, whereas one instrument based on light scattering overestimated the MCHC. These results indicate that inaccuracies in the measured mean cell volume (MCV) of dehydrated or otherwise undeformable cells may lead to spurious values for MCHC when electronic cell counters are used.

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Predictive Value of Cord Blood Hematological Indices and Hemoglobin Barts for the Detection of Heterozygous α-Thalassemia-2 in an African-Caribbean Population

Clinical Chemistry ◽

10.1093/clinchem/45.9.1495 ◽

1999 ◽

Vol 45 (9) ◽

pp. 1495-1500 ◽

Cited By ~ 1

Author(s):

Fey PL van der Dijs ◽

Marcel Volmer ◽

Dieuwke G van Gijssel-Wiersma ◽

Jan W Smit ◽

Reind van Veen ◽

...

Keyword(s):

Logistic Regression ◽

Cord Blood ◽

Predictive Value ◽

Dna Analysis ◽

Hemoglobin Concentration ◽

Mean Cell Volume ◽

Hematological Indices ◽

Netherlands Antilles ◽

African Caribbean ◽

Mean Cell Hemoglobin

Abstract Background: Cord blood hemoglobin Barts (HbBarts) and hemocytometric indices may be used for classification of newborns into those without α-thalassemia-2 (αα/αα) and with heterozygous α-thalassemia-2 (-α3.7/αα). We investigated by logistic regression analysis whether the combination of HbBarts and hemocytometric indices improves classification compared with classification based on a single analyte. Methods: HbBarts percentages and hemocytometric indices were determined in cord blood of 208 consecutive newborns in Curaçao (Netherlands Antilles). Of these, 157 had αα/αα and 51 had -α3.7/αα, as established by DNA analysis. Results: Between-group differences were significant for erythrocytes, mean cell volume, mean cell hemoglobin (MCH), mean cell hemoglobin concentration, platelets, hemoglobin F0 (HbF0), and HbBarts. The Logit equation of the logistic regression model, using MCH (pg) and HbBarts (%), was: 42.7164 + 5.7916(HbBarts) − 1.3110(MCH). A sensitivity of 100% was reached at a Logit value of −3.70. The corresponding specificity was 62.2%, and the predictive value of a positive test (PV+) was 46.3% (95% confidence interval, 37.0–55.7%). The relative information gains were as follows: 88% for the HbBarts-MCH combination, 26% for MCH (not significant), and 0% for HbBarts compared with the 24.6% -α3.7/αα prevalence. Conclusion: Combined use of cord blood HbBarts and MCH improves classification compared with classification based on single hemocytometric indices.

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Measurement of the Cellular Hemoglobin Concentration by Laser Scatter Method from Excessive Lipemic Sample: CASE REPORT

Combinatorial Chemistry & High Throughput Screening ◽

10.2174/1386207322666190925130114 ◽

2019 ◽

Vol 22 (7) ◽

pp. 502-505

Author(s):

Ataman Gönel ◽

Ismail Koyuncu

Keyword(s):

Venous Blood ◽

Hemoglobin Concentration ◽

Female Child ◽

Acute Tonsillitis ◽

Scatter Method ◽

Normal Limits ◽

Pediatric Clinic ◽

Automated Hematology Analyzer ◽

Familial Hyperlipidemia ◽

Sample Case

A 33-month old female child presented at a pediatric clinic with acute tonsillitis, and it was subsequently discovered that she had familial hyperlipidemia. Measurement of the patient’s whole blood tests was performed by a multiparameter automated hematology analyzer, the CELLDYN Ruby System® (Abbott, Lake Forest, USA) using venous blood extracted from a tube containing 3.0 mL of EDTA. Although her hematocrit levels were within normal limits, the hemoglobin (Hgb) level, mean corpuscular volume (MCH) and mean corpuscular Hgb concentration (MCHC) could not be determined using the spectrophotometric method. The results of these tests could not be measured when repeated using dilution. When the sample was left to rest for several minutes, it was observed to be excessively lipemic. The measurements were repeated using the Alinity HQ Analyzer® (Abbott), which determines Hgb concentration using laser scatter and spectrophotometry. Hgb cellular concentration was incorrectly measured as being 21.9 mg/dL using routine spectrophotometry (denoted by a flag indicating Hgb interference) and correctly found to be 10.8 mg/dL. Thus, in samples of excessive lipemia, Hgb, MCH, and MCHC levels cannot be measured accurately using spectrophotometry. Hematology analyzers that can measure cellular hemoglobin (cHGB) and average erythrocyte hemoglobin concentration (cHCM) by laser scatter method may be recommended when analyzing a blood sample that contains excessive lipemia.

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