Goat milk with and without increased concentrations of lysozyme improves repair of intestinal cell damage induced by enteroaggregative Escherichia coli

The metabolism of spoilage organism in the seafood is a major cause of fish spoilage. Lipid oxidation leads to production of intermediate peroxides causing bad odor and offflavour producing compounds. Hence, based on the hurdle technology method and applications of nanotechnology, the inhibitory effects of copper oxide nanoparticles coupled with vitamin-E (CuONPs+VE) was investigated with the aim of controlling the oxidative cell damage in seafood by estimating Reacting Oxygen Species (ROS) using 2’,7’- dichlorodihydrofluorescein diacetate (DCF) and preventing microbial spoilage by studying the cell viability using MTT assay in the present study. The MTT assay reveals that the CuONPs+VE inhibited the metabolic activity of spoilage bacteria which was confirmed from the inhibition percentage values against the test organisms. The CuONPs+VE exposed Escherichia coli reduced upto 86 ± 0.57% after enumerating the CFU in a Plate Count Agar media. Salmonella sp and Shigella sp exhibited 89 ± 1.04% and 85 ± 0.25% respectively. The qualitative inhibitory effect of Nanometal vitamin complex against three test bacteria, were evaluated by agar diffusion method. Among the four different concentrations of Nanometal vitamin complex, the higher concentration (4X – 40μg/ml) exhibited maximum inhibitory effects in terms of zone of clearance. Against Escherichia coli, 12.3 ± 1.25mm of inhibitory zones were found evident. Salmonella sp showed 15.6 ± 0.57mm of inhibitory zones and Shigella sp revealed the inhibition zone of about 16.6 ± 1.04mm. The anti-oxidative defence mechanisms operating in the cells for scavenging of ROS was performed using DCF. The cells which are pre-incubated with CuONPs+VE and then exposed to H2O2 show insignificant green emission indicating a clear advantage of the nanoparticle form of vitamin-E (CuONPs+VE) in preventing oxidative cell death.

Download Full-text

Cell damage detection using Escherichia coli reporter plasmids: fluorescent and colorimetric assays

Archives of Microbiology ◽

10.1007/s00203-015-1119-y ◽

2015 ◽

Vol 197 (6) ◽

pp. 815-821 ◽

Cited By ~ 6

Author(s):

Felipe Padilla-Martínez ◽

Luz Adriana Carrizosa-Villegas ◽

Ángeles Rangel-Serrano ◽

Itzel Paramo-Pérez ◽

Verónica Mondragón-Jaimes ◽

...

Keyword(s):

Escherichia Coli ◽

Damage Detection ◽

Cell Damage ◽

Colorimetric Assays

Download Full-text

Serologic Markers of Systemic Immune Activation and Intestinal Cell Damage in Non-Celiac Wheat Sensitivity

Gastroenterology ◽

10.1016/s0016-5085(17)30487-0 ◽

2017 ◽

Vol 152 (5) ◽

pp. S37 ◽

Cited By ~ 1

Author(s):

Melanie Uhde ◽

Giacomo Caio ◽

Roberto De Giorgio ◽

Peter H.R. Green ◽

Umberto Volta ◽

...

Keyword(s):

Immune Activation ◽

Cell Damage ◽

Intestinal Cell ◽

Systemic Immune Activation ◽

Serologic Markers ◽

Wheat Sensitivity

Download Full-text

Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

Research Society and Development ◽

10.33448/rsd-v10i12.20735 ◽

2021 ◽

Vol 10 (12) ◽

pp. e596101220735

Author(s):

Iara Nunes de Siqueira ◽

Aline Antas Cordeiro Cavalcanti ◽

Joyce Galvão de Souza ◽

Filipe Jordão Pereira de Medeiros ◽

João Carlos Taveira ◽

...

Keyword(s):

Escherichia Coli ◽

Listeria Monocytogenes ◽

Pathogenic Bacteria ◽

Goat Milk ◽

Salmonella Spp ◽

Pasteurized Milk ◽

Enterococcus Spp ◽

Bacillus Spp ◽

Thermotolerant Coliforms ◽

Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba. Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

Download Full-text

Incidence of Escherichia coli  - Glucuronidase Positive on Goat Milk

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:12428 ◽

2016 ◽

Vol 73 (2) ◽

pp. 359

Author(s):

Zorica Voşgan ◽

Anca Domuţa ◽

Stela Jelea ◽

Lucia Mihălescu ◽

Flavia Pop

Keyword(s):

Escherichia Coli ◽

Food Poisoning ◽

Goat Milk ◽

Lactation Period ◽

Health Indicator ◽

Pathogenic Factor ◽

E Coli ◽

Higher Temperature ◽

Hot Weather

Papers on beta- glucuronidase sensitivity and specificity for identifying Escherichia coli in sources of environment, food, water, etc. have been published since 1976. In this study we conducted a review of the incidence of E. coli β- glucuronidase -positive in goat milk, obtained by hand milking throughout the lactation: spring, summer, autumn. The presence of E. coli in milk is considered both as a health indicator and a pathogenic factor capable of causing food poisoning. The determination of the E. coli β-glucuronidase-positive was carried using TBX medium by cultivating colonies typical blue at 440C. The absence of E. coli in milk yielded during the spring, when the animal milking is done three times a day, was found in the performed analyses; the same was observed during fall, when the milk production is lower and the milking is done once a day. The load of E. coli β-glucuronidase-positive was averaging 66.67 CFU/ml of goat milk, during the middle lactation period (July-August), in conditions of higher temperature. During this period, milking is done in the mountain zone, where the transhumance of animals takes place in summer. The presence of the species E. coli was also confirmed by microscopic examination. Attention should be paid to hygiene and milk should be immediately cooled, during hot weather, as E. coli can be a source of food poisoning.

Download Full-text

Effect of Gamma Radiation and Oregano Essential Oil on Murein and ATP Concentration of Escherichia coli O157:H7

Journal of Food Protection ◽

10.4315/0362-028x-68.12.2571 ◽

2005 ◽

Vol 68 (12) ◽

pp. 2571-2579 ◽

Cited By ~ 24

Author(s):

STÉPHANE CAILLET ◽

FRANÇOIS SHARECK ◽

MONIQUE LACROIX

Keyword(s):

Escherichia Coli ◽

Essential Oil ◽

Gamma Radiation ◽

Cell Damage ◽

Wall Structure ◽

High Dose ◽

Escherichia Coli O157 ◽

Oregano Essential Oil ◽

Intracellular Atp ◽

Oregano Oil

This study was carried out to evaluate the effects of gamma radiation alone or in combination with oregano essential oil on the murein composition of Escherichia coli O157:H7 and on the intracellular and extracellular concentrations of ATP. The bacterial strain was treated with three radiation doses: 0.4 kGy to induce cell damage, 1.1 kGy to obtain a viable but nonculturable state, and 1.3 kGy to cause cell death. Oregano essential oil was used at 0.006 and 0.025% (wt/vol), which is the MIC. All treatments had a significant effect (P ≤ 0.05) on the murein composition, although some muropeptides did not seem to be affected by the treatment. Each treatment had a different effect on the relative percentage and number of muropeptides. There was a significant correlation (P ≤ 0.05) between the decrease in intracellular ATP and the increase in extracellular ATP following treatment of the cells with oregano oil. The reduction of intracellular ATP was even more important when oregano oil was combined with irradiation, but irradiation alone at a high dose (≤1.1 kGy) significantly decreased (P ≤ 0.05) the internal ATP without affecting the external ATP. Transmission electron microscopic examination revealed that oregano oil and irradiation have an effect on cell wall structure.

Download Full-text

Persistent Systemic Microbial Translocation, Inflammation, and Intestinal Damage During Clostridioides difficile Infection

Open Forum Infectious Diseases ◽

10.1093/ofid/ofz507 ◽

2019 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Alessandra Oliva ◽

Lucia Aversano ◽

Massimiliano De Angelis ◽

Maria Teresa Mascellino ◽

Maria Claudia Miele ◽

...

Keyword(s):

Fecal Microbiota Transplantation ◽

Cell Damage ◽

Binding Protein ◽

Fecal Microbiota ◽

Microbial Translocation ◽

Intestinal Cell ◽

Intestinal Damage ◽

Fatty Acid Binding ◽

Clostridioides Difficile ◽

Clostridioides Difficile Infection

Abstract Background Clostridioides difficile infection (CDI) might be complicated by the development of nosocomial bloodstream infection (n-BSI). Based on the hypothesis that alteration of the normal gut integrity is present during CDI, we evaluated markers of microbial translocation, inflammation, and intestinal damage in patients with CDI. Methods Patients with documented CDI were enrolled in the study. For each subject, plasma samples were collected at T0 and T1 (before and after CDI therapy, respectively), and the following markers were evaluated: lipopolysaccharide-binding protein (LPB), EndoCab IgM, interleukin-6, intestinal fatty acid binding protein (I-FABP). Samples from nonhospitalized healthy controls were also included. The study population was divided into BSI+/BSI- and fecal microbiota transplantation (FMT) +/FMT- groups, according to the development of n-BSI and the receipt of FMT, respectively. Results Overall, 45 subjects were included; 8 (17.7%) developed primary n-BSI. Markers of microbial translocation and intestinal damage significantly decreased between T0 and T1, however, without reaching values similar to controls (P < .0001). Compared with BSI-, a persistent high level of microbial translocation in the BSI+ group was observed. In the FMT+ group, markers of microbial translocation and inflammation at T1 tended to reach control values. Conclusions CDI is associated with high levels of microbial translocation, inflammation, and intestinal damage, which are still present at clinical resolution of CDI. The role of residual mucosal perturbation and persistence of intestinal cell damage in the development of n-BSI following CDI, as well as the possible effect of FMT in the restoration of mucosal integrity, should be further investigated.

Download Full-text

Comparison of intense pulsed light- and ultraviolet (UVC)-induced cell damage in Listeria monocytogenes and Escherichia coli O157:H7

Food Control ◽

10.1016/j.foodcont.2011.11.032 ◽

2012 ◽

Vol 25 (2) ◽

pp. 654-659 ◽

Cited By ~ 62

Author(s):

Chan-Ick Cheigh ◽

Mi-Hyun Park ◽

Myong-Soo Chung ◽

Jung-Kue Shin ◽

Young-Seo Park

Keyword(s):

Escherichia Coli ◽

Listeria Monocytogenes ◽

Cell Damage ◽

Intense Pulsed Light ◽

Escherichia Coli O157 ◽

Pulsed Light

Download Full-text

Afa/Dr Diffusely Adhering Escherichia coli Infection in T84 Cell Monolayers Induces Increased Neutrophil Transepithelial Migration, Which in Turn Promotes Cytokine-Dependent Upregulation of Decay-Accelerating Factor (CD55), the Receptor for Afa/Dr Adhesins

Infection and Immunity ◽

10.1128/iai.71.4.1774-1783.2003 ◽

2003 ◽

Vol 71 (4) ◽

pp. 1774-1783 ◽

Cited By ~ 47

Author(s):

Frederic Betis ◽

Patrick Brest ◽

Véronique Hofman ◽

Julie Guignot ◽

Imad Kansau ◽

...

Keyword(s):

Escherichia Coli ◽

Laboratory Strain ◽

Intestinal Cell ◽

Necrosis Factor Alpha ◽

Decay Accelerating Factor ◽

E Coli ◽

Intestinal Cell Line ◽

Bowel Diseases ◽

Escherichia Coli Infection ◽

Human Intestinal Cell Line

ABSTRACT Ulcerative colitis and Crohn's disease are inflammatory bowel diseases thought to involve strains of Escherichia coli. We report here that two wild-type Afa/Dr diffusely adhering E. coli (DAEC) strains, C1845 and IH11128, which harbor the fimbrial F1845 adhesin and the Dr hemagglutinin, respectively, and the E. coli laboratory strain HB101, transformed with the pSSS1 plasmid to produce Afa/Dr F1845 adhesin, all induced interleukin-8 (IL-8) production and transepithelial migration of polymorphonuclear leukocytes (PMNL) in polarized monolayers of the human intestinal cell line T84 grown on semipermeable filters. We observed that after PMNL migration, expression of decay-accelerating factor (DAF, or CD55), the brush border-associated receptor for Afa/Dr adhesins, was strongly enhanced, increasing the adhesion of Afa/Dr DAEC bacteria. When examining the mechanism by which DAF expression was enhanced, we observed that the PMNL transepithelial migration induced epithelial synthesis of tumor necrosis factor alpha and IL-1β, which in turn promoted the upregulation of DAF.

Download Full-text