scholarly journals Comprehensive analysis of mRNAs and miRNAs in the ovarian follicles of uniparous and multiple goats at estrus phase

BMC Genomics ◽  
2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Xian Zou ◽  
Tingting Lu ◽  
Zhifeng Zhao ◽  
Guangbin Liu ◽  
Zhiquan Lian ◽  
...  
Keyword(s):  
Ovarian Follicles ◽  
Comprehensive Analysis ◽  
Estrus Phase

scholarly journals Combined iDISCO and CUBIC tissue clearing and lightsheet microscopy for in toto analysis of the adult mouse ovary†

2020 ◽  
Vol 102 (5) ◽  
pp. 1080-1089 ◽  
Author(s):  
Jennifer McKey ◽  
Lisa A Cameron ◽  
Devon Lewis ◽  
Iordan S Batchvarov ◽  
Blanche Capel
Keyword(s):  
Gold Standard ◽  
Computational Models ◽  
Adult Mouse ◽  
Ovarian Follicles ◽  
Comprehensive Analysis ◽  
Growth Stages ◽  
Mouse Ovary ◽  
3D Images ◽  
Primordial Follicles ◽  
Tissue Clearing

Abstract At any given time, the ovary contains a number of follicles in distinct growth stages, each with a set of identifying characteristics. Although follicle counting and staging using histological stains on paraffin-embedded ovary sections has been the gold standard in assessing ovarian health in fertility studies, the final counts rely on extrapolation factors that diverge greatly among studies. These methods also limit our ability to investigate spatial aspects of ovary organization. Recent advances in optical tissue clearing and lightsheet microscopy have permitted comprehensive analysis of intact tissues. In this study, we set out to determine the best clearing and imaging methods to generate 3D images of the complete adult mouse ovary that could be used for accurate assessments of ovarian follicles. We found that a combination of iDISCO and CUBIC was the best method to clear the immunostained ovary. Using lightsheet microscopy, we generated 3D images of the intact ovary and performed qualitative assessments of follicles at all stages of development. This study is an important step toward developing quantitative computational models that allow rapid and accurate assessments of growing and quiescent primordial follicles, and to investigate the integrity of extrinsic ovarian components including vascular and neuronal networks.

scholarly journals Comprehensive Analysis of mRNAs and miRNAs in the Ovarian Follicles of Uniparous and Multiple Goats at Estrus Phase

2019 ◽  
Author(s):  
Xian Zou ◽  
Tingting Lu ◽  
Zhifeng Zhao ◽  
Guangbin Liu ◽  
Zhiquan Lian ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Steroid Hormone ◽  
Follicular Development ◽  
Interaction Network ◽  
Ovarian Follicles ◽  
Rna Seq ◽  
Steroid Hormone Biosynthesis ◽  
Hormone Biosynthesis ◽  
Meat Goat ◽  
Estrus Phase

Abstract Background Fertility is an important economic trait in production of meat goat, and follicular development plays an important role in fertility. Despite many mRNAs and microRNAs (miRNAs) have been found in playing critical roles in ovarian biological processes, the interactions between mRNAs and miRNAs in follicular development is not yet completely understood. In addition, less attention has been given to the single follicle (dominant or atretic follicle) in goat. The study was aimed to identify mRNAs, miRNAs and signaling pathways as well as their interaction networks in the ovarian follicles (large follicles and small follicles) of multiple and uniparous goats (Chuanzhong Black Goats) at estrus phase by using a deep RNA-sequencing (RNA-seq) method.Results The result showed that there were more large follicles in multiple than in uniparous goats ( P <0.05), while no difference was observed in small follicles between them ( P >0.05). For the small follicles of multiple and uniparous goats at estrus phase, 289 differentially expressed mRNAs (DEmRNAs) and 16 DEmiRNAs were identified; and for the large follicles, 195 DEmRNAs and 7 DEmiRNAs were identified. Ovarian steroidogenesis and steroid hormone biosynthesis were significantly enriched in small follicles, while ABC transporters and steroid hormone biosynthesis in large follicles. The results of qRT-PCR were generally consistent with the RNA-seq data. The mRNA-miRNA interaction network showed that CD36 (miR-122, miR-200a, miR-141), TNFAIP6 (miR-141, miR-200a, miR-182), CYP11A1 (miR-122), SERPINA5 (miR-1, miR-206, miR-133a-3p, miR-133b) and PTGFR (miR-182, miR-122) might be related to fertility, but need further verification.Conclusion This study provides the first identification of the DEmRNAs and DEmiRNAs as well as their interactions in the follicles of multiple and uniparous goats at estrus phase by using RNA-seq technology. These analyses provide new clues to uncover molecular mechanisms and signaling networks of goat reproduction which could be potentially used to increase ovulation rate and kidding rate in goat.

Sozial-räumliche Dynamiken der Agrartreibstoffe.

2016 ◽  
Vol 46 (184) ◽  
pp. 423-440 ◽  
Author(s):  
Kristina Dietz ◽  
Bettina Engels ◽  
Oliver Pye
Keyword(s):  
Political Economy ◽  
Capital Flows ◽  
Spatial Dynamics ◽  
Financial Sector ◽  
Comprehensive Analysis ◽  
Spatial Theory ◽  
Global Political Economy

This article explores the spatial dynamics of agrofuels. Building on categories from the field of critical spatial theory, it shows how these categories enable a comprehensive analysis of the spatial dynamics of agrofuels that links the macro-structures of the global political economy to concrete, place-based struggles. Four core socio-spatial dynamics of agrofuel politics are highlighted and applied to empirical findings: territorialization, the financial sector as a new scale of regulation, place-based struggles and transnational spaces of resources and capital flows.

Concerning the abuse of power by an official

2020 ◽  
pp. 103-110
Author(s):  
Ihor Oheruk
Keyword(s):  
Criminal Code ◽  
Comprehensive Analysis ◽  
Practical Significance ◽  
Abuse Of Power ◽  
Government Functions ◽  
Analysis And Synthesis ◽  
The Status ◽  
The Subject ◽  
Criminal Legislation ◽  
Logical Semantic

Purpose. The purpose of the work is to analyze the application of the second and third parts of Article 3692 of the Criminal Code of Ukraine to officials in the context, that defines them by the Criminal Code of Ukraine in the note to Article 364 of the Criminal Code of Ukraine. Methodology. The methodology includes a comprehensive analysis and synthesis of the available scientific and theoretical material and the formulation of relevant conclusions and recommendations. In the course of the study, the following methods of scientific knowledge were used: terminological, logical-semantic, system-structural, logical-normative. Results: in the course of research the cause of criminalization of such act as "abuse of power" is considered, the subject of the specified criminal act which has the features of "an official" in the context, that defines it by the note to Article 364 of the Criminal Code of Ukraine is analyzed and the main ways of committing criminal acts, that are provided for in this article of the Criminal Code of Ukraine are identified. Originality. The study found, that one of the key conditions for the opportunity to influence officials, that are authorized to perform government or local self-government functions, is the position held by the official and the related opportunities. Therefore, taking into account the opinion of the scientists, that the subject of crimes, that are provided for by the second and third parts of Article 3692 is special, the peculiarities of which is the cumulative feature, that denotes, that such person is not endowed with the status of an official, well-founded need to specify the criminal legislation of Ukraine in terms of the application the second and third parts of Article 3692 of the Criminal code of Ukraine concerning officials in the context, that defines them by the criminal legislation of Ukraine in the note to Article 364 of the Criminal Code of Ukraine. Practical significance. The research results can be used in lawmaking in the improvement of anti-corruption legislation.

BASIC FUNDAMENTALS OF CIVIL LAW AND THEIR IMPORTANCE

2019 ◽  
pp. 120-126
Author(s):  
Diana Vivcharuk
Keyword(s):  
Law Enforcement ◽  
Scientific Knowledge ◽  
Civil Law ◽  
Comprehensive Analysis ◽  
Practical Significance

Purpose. The purpose of the article is the regulation of relations on the principles of civil law. Methodology. The methodology includes a comprehensive analysis and a synthesis of available scientific and theoretical information. It is includes the formulation of relevant conclusions and recommendations. Such methods of scientific knowledge were used: terminological, functional, systemic-structural, logical-normative. Results: it was determined, that principles of civil law – an ideas of the civil law, that characterized by systematic,versatile, more stable, more regylated. Originality. An article is the special reseach that explores the problems of civil law in Ukraine. Practical significance. The results of the research can be used in legislation and law-enforcement activities.

AKAD FINANCING OF MURABAHAH BETWEEN CUSTOMERS WITH MUAMALAT BANKS HARKAT VIEWED FROM SHARI'AH PRINCIPLES

2020 ◽  
Vol 10 (1) ◽  
pp. 13-26
Author(s):  
Candra Irawan ◽  
Adi Bastian ◽  
Febrozi Rohadi
Keyword(s):  
Data Collection ◽  
Financial Institutions ◽  
Research Method ◽  
Economic Condition ◽  
Field Research ◽  
Comprehensive Analysis ◽  
Islamic Banks ◽  
Legal Research ◽  
Trading System ◽  
Library Research

Currently in Indonesia Islamic Bank has gained a place and interested in the community, causing many emerging Syari'ah Bank and Financial Institutions of the syari'ah, and products in Islamic banks are widely used is murabahah financing. The formulation of the problem in this research are: (1). How is the implementation of the sale and purchase through murabahah financing between Bank Muamalat Harkat with customers. (2). Is trading system murabahah financing between Bank Muamalat Harkat and customers have been according to the principles of Syari’ah. (3). How murabahah financing efforts to resolve the breach between the customer and Muamalat Harkat. This research method is empirical legal research, this study was conducted in Bank Muamalat Harkat based data collection through field research such as interviews, observation and description as well as information from respondents through library research. The results of this research are: before an agreement Bank to assess carefully the prospective customer in the form of a comprehensive analysis and is divided into several stages, such as the assessment using the principle of 5C Character (Character of the debitor), Chapacity (Capability Candidate Debitor) , Capital (Capital candidate Debitor), Collateral (Collateral candidate Debitor) and Condition of economy (economic condition of the prospective Borrower). Trading system murabahah financing between Bank Muamalat Harkat with the customer has not fully based on the principles of the Syari'ah. Murabahah financing efforts to resolve the defaults can be solved by R3 is Restrukturing (Arrangement Back), Reconditioning (Terms Back) and Rescheduling (rescheduling), sales collateral and auction execution. 

Concerning the Concept of Light-Colour Arrangement of the Urban Environment in the Central Part of Tyumen

2020 ◽  
pp. 34-42
Author(s):  
Nikolay I. Shchepetkov ◽  
Svetlana B. Kapeleva ◽  
Denis V. Bugaev ◽  
Gregory S. Matovnikov ◽  
Anna S. Kostareva
Keyword(s):  
Urban Environment ◽  
Comprehensive Analysis ◽  
Assessment Criteria ◽  
Field Observations ◽  
General Plan ◽  
Design Solutions ◽  
Methodological Principles ◽  
The City

The article provides a comprehensive analysis of outdoor lighting in the central part of Tyumen (with consideration of conducted field observations) and prospects of its development on the basis of the general plan of illumination of the central part of the city being under design. Main provisions of this general plan as well as methodological principles and assessment criteria of design solutions illustrat-ed by photographs, schemes and visualisations of the illuminated objects are described.

Evaluation of epidemiological data on effect of therapy with Ipigrix® on motor and sensory functions in ambulatory patients with various diseases ofperipheral nervous system

2019 ◽  
Vol 1 (2) ◽  
pp. 11-14
Author(s):  
O. S. Levin ◽  
O. V. Matvievskaya
Keyword(s):  
Nervous System ◽  
Observational Study ◽  
Peripheral Nervous System ◽  
Epidemiological Data ◽  
Comprehensive Analysis ◽  
Ambulatory Patients ◽  
Effect Of Therapy ◽  
Sensory Functions

The article contains a comprehensive analysis of the summary epidemiological data obtained during the observational study to assess the effect of therapy with Ipigrix® on the dynamics of motor and sensory functions, as well as the severity of pain in outpatient patients with various diseases of the peripheral nervous system: mononeuropathy, polyneuropathy and polyradiculopathy of various origins.

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