scholarly journals Mapping pre-harvest sprouting resistance loci in AAC Innova × AAC Tenacious spring wheat population

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Raman Dhariwal ◽  
Colin W. Hiebert ◽  
Mark E. Sorrells ◽  
Dean Spaner ◽  
Robert J. Graf ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Wheat Yield ◽  
Seed Viability ◽  
Pedigree Analysis ◽  
Resistance Qtls ◽  
Wheat Population ◽  
End Use ◽  
Sprouting Resistance ◽  
First Time ◽  
Chromosome 3B

Abstract Background Pre-harvest sprouting (PHS) is a major problem for wheat production due to its direct detrimental effects on wheat yield, end-use quality and seed viability. Annually, PHS is estimated to cause > 1.0 billion USD in losses worldwide. Therefore, identifying PHS resistance quantitative trait loci (QTLs) is crucial to aid molecular breeding efforts to minimize losses. Thus, a doubled haploid mapping population derived from a cross between white-grained PHS susceptible cv AAC Innova and red-grained resistant cv AAC Tenacious was screened for PHS resistance in four environments and utilized for QTL mapping. Results Twenty-one PHS resistance QTLs, including seven major loci (on chromosomes 1A, 2B, 3A, 3B, 3D, and 7D), each explaining ≥10% phenotypic variation for PHS resistance, were identified. In every environment, at least one major QTL was identified. PHS resistance at most of these loci was contributed by AAC Tenacious except at two loci on chromosomes 3D and 7D where it was contributed by AAC Innova. Thirteen of the total twenty-one identified loci were located to chromosome positions where at least one QTL have been previously identified in other wheat genotype(s). The remaining eight QTLs are new which have been identified for the first time in this study. Pedigree analysis traced several known donors of PHS resistance in AAC Tenacious genealogy. Comparative analyses of the genetic intervals of identified QTLs with that of already identified and cloned PHS resistance gene intervals using IWGSC RefSeq v2.0 identified MFT-A1b (in QTL interval QPhs.lrdc-3A.1) and AGO802A (in QTL interval QPhs.lrdc-3A.2) on chromosome 3A, MFT-3B-1 (in QTL interval QPhs.lrdc-3B.1) on chromosome 3B, and AGO802D, HUB1, TaVp1-D1 (in QTL interval QPhs.lrdc-3D.1) and TaMyb10-D1 (in QTL interval QPhs.lrdc-3D.2) on chromosome 3D. These candidate genes are involved in embryo- and seed coat-imposed dormancy as well as in epigenetic control of dormancy. Conclusions Our results revealed the complex PHS resistance genetics of AAC Tenacious and AAC Innova. AAC Tenacious possesses a great reservoir of important PHS resistance QTLs/genes supposed to be derived from different resources. The tracing of pedigrees of AAC Tenacious and other sources complements the validation of QTL analysis results. Finally, comparing our results with previous PHS studies in wheat, we have confirmed the position of several major PHS resistance QTLs and candidate genes.

scholarly journals Seeding Rate Effects on Hybrid Spring Wheat Yield, Yield Components and Quality

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1240
Author(s):  
Peder K. Schmitz ◽  
Joel K. Ransom
Keyword(s):  
Spring Wheat ◽  
Yield Components ◽  
Wheat Yield ◽  
Triticum Aestivum L ◽  
Yield Component ◽  
Economic Feasibility ◽  
Protein Yield ◽  
Seeding Rate ◽  
Hard Red Spring Wheat ◽  
End Use

Agronomic practices, such as planting date, seeding rate, and genotype, commonly influence hard red spring wheat (HRSW, Triticum aestivum L. emend. Thell.) production. Determining the agronomic optimum seeding rate (AOSR) of newly developed hybrids is needed as they respond to seeding rates differently from inbred cultivars. The objectives of this research were to determine the AOSR of new HRSW hybrids, how seeding rate alters their various yield components, and whether hybrids offer increased end-use quality, compared to conventional cultivars. The performance of two cultivars (inbreds) and five hybrids was evaluated in nine North Dakota environments at five seeding rates in 2019−2020. Responses to seeding rate for yield and protein yield differed among the genotypes. The AOSR ranged from 3.60 to 5.19 million seeds ha−1 and 2.22 to 3.89 million seeds ha−1 for yield and protein yield, respectively. The average AOSR for yield for the hybrids was similar to that of conventional cultivars. However, the maximum protein yield of the hybrids was achieved at 0.50 million seeds ha−1 less than that of the cultivars tested. The yield component that explained the greatest proportion of differences in yield as seeding rates varied was kernels spike−1 (r = 0.17 to 0.43). The end-use quality of the hybrids tested was not superior to that of the conventional cultivars, indicating that yield will likely be the determinant of the economic feasibility of any future released hybrids.

scholarly journals Transcriptomic profiling of wheat near-isogenic lines reveals candidate genes on chromosome 3A for pre-harvest sprouting resistance

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xingyi Wang ◽  
Hui Liu ◽  
Kadambot H. M. Siddique ◽  
Guijun Yan
Keyword(s):  
Candidate Genes ◽  
Quantitative Trait ◽  
Genomic Region ◽  
Nucleotide Polymorphisms ◽  
Near Isogenic Lines ◽  
Gene Effects ◽  
Isogenic Lines ◽  
Trait Locus ◽  
Grain Yield And Quality ◽  
Sprouting Resistance

Abstract Background Pre-harvest sprouting (PHS) in wheat can cause severe damage to both grain yield and quality. Resistance to PHS is a quantitative trait controlled by many genes located across all 21 wheat chromosomes. The study targeted a large-effect quantitative trait locus (QTL) QPhs.ccsu-3A.1 for PHS resistance using several sets previously developed near-isogenic lines (NILs). Two pairs of NILs with highly significant phenotypic differences between the isolines were examined by RNA sequencing for their transcriptomic profiles on developing seeds at 15, 25 and 35 days after pollination (DAP) to identify candidate genes underlying the QTL and elucidate gene effects on PHS resistance. At each DAP, differentially expressed genes (DEGs) between the isolines were investigated. Results Gene ontology and KEGG pathway enrichment analyses of key DEGs suggested that six candidate genes underlie QPhs.ccsu-3A.1 responsible for PHS resistance in wheat. Candidate gene expression was further validated by quantitative RT-PCR. Within the targeted QTL interval, 16 genetic variants including five single nucleotide polymorphisms (SNPs) and 11 indels showed consistent polymorphism between resistant and susceptible isolines. Conclusions The targeted QTL is confirmed to harbor core genes related to hormone signaling pathways that can be exploited as a key genomic region for marker-assisted selection. The candidate genes and SNP/indel markers detected in this study are valuable resources for understanding the mechanism of PHS resistance and for marker-assisted breeding of the trait in wheat.

scholarly journals Novel Quantitative Trait Loci for Grain Cadmium Content Identified in Hard White Spring Wheat

2021 ◽  
Vol 12 ◽  
Author(s):  
Ling Qiao ◽  
Justin Wheeler ◽  
Rui Wang ◽  
Kyle Isham ◽  
Natalie Klassen ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Candidate Gene ◽  
Spring Wheat ◽  
Quantitative Trait ◽  
Field Trials ◽  
Cadmium Content ◽  
Wheat Population ◽  
Trait Loci ◽  
Multiple Field ◽  
First Time

Cadmium (Cd) is a heavy metal that can cause a variety of adverse effects on human health, including cancer. Wheat comprises approximately 20% of the human diet worldwide; therefore, reducing the concentrations of Cd in wheat grain will have significant impacts on the intake of Cd in food products. The tests for measuring the Cd content in grain are costly, and the content is affected significantly by soil pH. To facilitate breeding for low Cd content, this study sought to identify quantitative trait loci (QTL) and associated molecular markers that can be used in molecular breeding. One spring wheat population of 181 doubled haploid lines (DHLs), which was derived from a cross between two hard white spring wheat cultivars “UI Platinum” (UIP) and “LCS Star” (LCS), was assessed for the Cd content in grain in multiple field trials in Southeast Idaho, United States. Three major QTL regions, namely, QCd.uia2-5B, QCd.uia2-7B, and QCd.uia2-7D, were identified on chromosomes 5B, 7B, and 7D, respectively. All genes in these three QTL regions were identified from the NCBI database. However, three genes related to the uptake and transport of Cd were used in the candidate gene analysis. The sequences of TraesCS5B02G388000 (TaHMA3) in the QCd.uia2-5B region and TraesCS7B02G320900 (TaHMA2) and TraesCS7B02G322900 (TaMSRMK3) in the QCd.uia2-7B region were compared between UIP and LCS. TaHMA2 on 7B is proposed for the first time as a candidate gene for grain Cd content in wheat. A KASP marker associated with this gene was developed and it will be further validated in near-isogenic lines via a gene-editing system in future studies.

Mapping of a QTL in Chromosome 3B for Grain Dormancy in White-Grained Wheat Population

2010 ◽  
Vol 1 (2) ◽  
Author(s):  
Nisha Sulari Kottearachchi ◽  
Shinichi Takao ◽  
Kiyoaki Kato ◽  
Hideho Miura
Keyword(s):  
Grain Dormancy ◽  
Wheat Population ◽  
Chromosome 3B

Influence of mixing energy on the solid-state behavior and clay fraction threshold of PA12/C30B® nanocomposites

2019 ◽  
Vol 39 (6) ◽  
pp. 565-572
Author(s):  
Nourredine Aït Hocine ◽  
Pascal Médéric ◽  
Hanaya Hassan
Keyword(s):  
Solid State ◽  
Mechanical Energy ◽  
Three Dimensional ◽  
Clay Fraction ◽  
Critical Value ◽  
Processing Conditions ◽  
State Behavior ◽  
Mixing Energy ◽  
End Use ◽  
First Time

Abstract This study focuses on the influence of mixing energy on the solid-state behavior and clay fraction threshold of nanocomposites. Thus, three polyamide12/clay (PA12/C30B®) nanocomposites exhibiting different nanostructures were prepared from three sets of processing conditions. Then, thermal and dynamical viscoelastic properties of these nanocomposites were analyzed, in relationship with the material nanostructure and processing conditions. For the first time, the solid-state properties of the nanocomposites revealed the existence of a critical specific mixing mechanical energy. Below this critical value, an increase of mechanical energy refines the structure, improving some end-use properties of the nanocomposite. Above this value, a high mixing energy supply is necessary in order to significantly modify the structure. They also highlighted that the clay fraction threshold, which is commonly attributed to the formation of a three-dimensional percolated network, decreases with increasing specific mixing energy, less significantly when this energy is superior to its critical value.

The Phenolyzer Suite: Prioritizing the Candidate Genes Involved in Microtia

2019 ◽  
Vol 128 (6) ◽  
pp. 556-562 ◽  
Author(s):  
Huang Xin ◽  
Wang Changchen ◽  
Liu Lei ◽  
Yang Meirong ◽  
Zhang Ye ◽  
...  
Keyword(s):  
Candidate Genes ◽  
High Throughput ◽  
Potential Candidate ◽  
Single Nucleotide Variants ◽  
Gene Score ◽  
Single Nucleotide ◽  
Research Directions ◽  
Score System ◽  
Pathogenic Genes ◽  
First Time

Objective: Microtia is a congenital malformation of the external ear. Great progress about the genetic of microtia has been made in recent years. This article was to prioritize the potential candidate pathogenic genes of microtia based on existing studies and reports, with the purpose of narrowing the range of following study scientifically and quickly. Method: A computational tool called Phenolyzer (phenotype-based gene analyzer) was used to prioritize microtia genes. Microtia, as a query term, was input in the interface of Phenolyzer. After several steps, including disease match, gene query, gene score system, seed gene growth, and gene ranking, the final results about genetic information of microtia were provided. Then we tracked details of the top 10 genes ranked by Phenolyzer on the basis of previous reports. Results: We detected 10 348 genes associated with microtia or related syndromes, and 78 genes of those genes belonged to seed genes. Every gene was given a score, and the gene with higher scores was more likely influence microtia. The top 10 ranked genes included HOXA2, CHD7, CDT1, ORC1, ORC4, ORC6, CDC6, MED12, TWIST1, and GLI3. Otherwise, four gene-gene interactions were displayed. Conclusion: This article prioritized candidate genes of microtia for the first time. High-throughput methods provide tens of thousands of single-nucleotide variants, indels, and structural variants, and only a handful are relevant to microtia or associated syndromes. Combine the ranked potential pathogenic genes list from Phenolyzer with the results of samples provided by high-throughput methods, and more precise research directions are presented.

Genetics of late maturity α-amylase in a doubled haploid wheat population

2010 ◽  
Vol 61 (2) ◽  
pp. 153 ◽  
Author(s):  
M. K. Tan ◽  
A. P. Verbyla ◽  
B. R. Cullis ◽  
P. Martin ◽  
A. W. Milgate ◽  
...  
Keyword(s):  
Doubled Haploid ◽  
Value Added ◽  
Wheat Breeding ◽  
Wheat Varieties ◽  
Breeding Lines ◽  
Positive Effects ◽  
Wheat Population ◽  
B1 Gene ◽  
Late Maturity ◽  
First Time

Late maturity α-amylase (LMA) in wheat is a defect where high-isoelectric point (pI) α-amylase accumulates in the ripening grain. Wheat genotypes vary in expression from zero to high levels of α-amylase, the latter with detrimental consequences on their use for value-added end products. Expression in each genotype is characterised by varying numbers of grains affected and different levels in each grain. Analysis of a doubled haploid (DH) population (188 lines) from WW1842 × Whistler has identified significant QTL on chromosomes 2DL, 3A, 3B, 3D, 4B, 4D, 5DS and 5BL. The 4B LMA allele (P < 0.0001) from Whistler is closely linked to the QTL for the ‘tall’ allele (P < 0.0001) of the Rht-B1 gene. The 4D LMA QTL (P < 0.0001) in WW1842 co-locates with the QTL for the ‘tall’ allele (P < 0.0001) of the Rht-D1 gene. This study has shown for the first time that a DH cross between two semi-dwarf cultivars with low or no LMA produces ~25% of progeny lines of the ‘tall’ genotypes with a high frequency of LMA. This is attributed to the large additive positive effects from the combination of one recessive ‘tall’ Rht-B1 gene and one recessive ‘tall’ Rht-D1 gene. High-yielding semi-dwarf genotypes with different combinations of Rht-B1 and Rht-D1 alleles which have very low or non-existent LMA expression (e.g. WW1842 and Whistler) may meet industry criteria for registration as commercial wheat varieties. However, when they are used as breeding lines, the cross produces some progeny genotypes with severe levels of LMA. These LMA genotypes comprise the gibberellic acid-sensitive ‘tall’ progenies and a very small proportion of semi-dwarfs. Thus, it is of paramount importance to screen the defect in wheat breeding programs. The suite of QTL identified for LMA will enable the use of marker assisted selection in the pyramiding of the beneficial QTL to maximise yield and minimise (or eliminate) LMA in semi-dwarf genotypes.

scholarly journals QTL mapping revealed TaVp-1A conferred pre-harvest sprouting resistance in wheat population Yanda 1817×Beinong 6

2017 ◽  
Vol 16 (2) ◽  
pp. 435-444 ◽  
Author(s):  
Sheng-hui ZHOU ◽  
Lin FU ◽  
Qiu-hong WU ◽  
Jiao-jiao CHEN ◽  
Yong-xing CHEN ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Wheat Population ◽  
Sprouting Resistance

Vesper hard red spring wheat

2013 ◽  
Vol 93 (2) ◽  
pp. 315-321 ◽  
Author(s):  
J. Thomas ◽  
S. Fox ◽  
B. McCallum ◽  
T. Fetch ◽  
J. Gilbert ◽  
...  
Keyword(s):  
Spring Wheat ◽  
High Water ◽  
Head Blight ◽  
Hard Red Spring Wheat ◽  
Wheat Midge ◽  
End Use ◽  
Sprouting Resistance ◽  
Moderately Resistant ◽  
Wheat Kernels

Thomas, J., Fox, S., McCallum, B., Fetch, T., Gilbert, J., Menzies, J., Wise, I., Smith, M., Gaudet, D., Niziol, D., Humphreys, G. and Brown, D. 2013. Vesper hard red spring wheat. Can. J. Plant Sci. 93: 315–321. Vesper is a high-yielding, hard red spring wheat that is adapted to the wheat-growing regions of Manitoba and Saskatchewan. In the Central Bread Wheat Cooperative Registration Trials of 2007, 2008 and 2009, Vesper out-yielded five check cultivars by an average of 12%, and Unity, which was the highest yielding check, by 4.6%. Lodging, height and maturity scores of Vesper were all intermediate (similar to the check mean). Pre-harvest sprouting resistance of Vesper was equivalent to the poorer checks (Katepwa and CDC Teal). Kernels of Vesper were heavier than all five checks and test weight was high (exceeded only by Unity). Vesper was resistant to leaf rust and was moderately resistant to stem rust and Fusarium head blight. Vesper was intermediately resistant to loose smut and was susceptible to common bunt. Spikes of Vesper showed two forms of resistance to wheat midge: antibiotic resistance (no larvae observed in the field) and antixenotic resistance (reduced egg numbers laid by caged ovipositing females). Over 3 yr of testing, end use quality of Vesper was rated as eligible for the Canada Western Red Spring (CWRS) market class of wheat. Kernels of Vesper were relatively hard with consequent high water absorption.

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