scholarly journals Interactions between genetics and environment shape Camelina seed oil composition

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jordan R. Brock ◽  
Trey Scott ◽  
Amy Yoonjin Lee ◽  
Sergei L. Mosyakin ◽  
Kenneth M. Olsen
Keyword(s):  
Genetic Diversity ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Seed Oil ◽  
Crop Improvement ◽  
Genotyping By Sequencing ◽  
Oilseed Crop ◽  
Environmental Niche ◽  
Oil Composition

Abstract Background Camelina sativa (gold-of-pleasure) is a traditional European oilseed crop and emerging biofuel source with high levels of desirable fatty acids. A twentieth century germplasm bottleneck depleted genetic diversity in the crop, leading to recent interest in using wild relatives for crop improvement. However, little is known about seed oil content and genetic diversity in wild Camelina species. Results We used gas chromatography, environmental niche assessment, and genotyping-by-sequencing to assess seed fatty acid composition, environmental distributions, and population structure in C. sativa and four congeners, with a primary focus on the crop’s wild progenitor, C. microcarpa. Fatty acid composition differed significantly between Camelina species, which occur in largely non-overlapping environments. The crop progenitor comprises three genetic subpopulations with discrete fatty acid compositions. Environment, subpopulation, and population-by-environment interactions were all important predictors for seed oil in these wild populations. A complementary growth chamber experiment using C. sativa confirmed that growing conditions can dramatically affect both oil quantity and fatty acid composition in Camelina. Conclusions Genetics, environmental conditions, and genotype-by-environment interactions all contribute to fatty acid variation in Camelina species. These insights suggest careful breeding may overcome the unfavorable FA compositions in oilseed crops that are predicted with warming climates.

scholarly journals Varietal Differences in the Oil Composition of the Seed of Two Indigenous Chrysophyllum albidium Species

2019 ◽  
pp. 1-7
Author(s):  
Bello, Adunola Abosede ◽  
Muniru, Oluwasegun Soliu ◽  
Igwe, Chima Cartney
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Seed Oil ◽  
Chemical Properties ◽  
Soxhlet Extraction ◽  
Major Fatty Acid ◽  
Seed Oils ◽  
Oil Composition ◽  
Federal Institute

Aim: To investigate the varietal difference in the composition of the oil of two Chryosophyllum albidium species. Study Design: Laboratory experimental design was used. Place and Duration of Study: Chryosophyllum acreanum and Chrysophyllum africana seed species of Chrysophyllum albidium were collected from Oja Oba market, Ibadan, Oyo State. The study was carried out between February 2019 - August 2019 at the Oilseed Laboratory of Federal Institute of Industrial Research, Oshodi, Lagos State, Nigeria. Methodology: Oil in both seeds was extracted using Soxhlet extraction method. The physical and chemical properties of the oils were determined using official methods of analysis while the fatty acid composition of the seed oils was analysed using Gas Chromatography- Mass Spectrophotometer. Results: The oil yield for both seeds was low, 3.52% for C. acreanum and 3.75% for C. africana. The values for the physical properties (Specific gravity, refractive index and unsaponifiable matter) of C. acreanum seed oil were higher than for C. africana seed oil. The chemical properties shows that the acid and peroxide values are 2.79mgKOH/g; 2.67mgKOH/g, 1.78mEq/kg; 1.63mEq/kg for C. acreanum and C. africana seed oil respectively while the iodine values for both seed oils are below 100mgI2/100g. The fatty acid composition shows that both seed oil contains myristic acid as their major fatty acid. Conclusion: The evaluated characteristics of the seed oils showed that there is no significant differences in the oil composition of C. albidium seed varieties as the oil composition are closely related except for the slight difference in their fatty acid profile.

scholarly journals Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content

2020 ◽  
Author(s):  
Alina Chernova ◽  
Rim Gubaev ◽  
Anupam Singh ◽  
Katrina Sherbina ◽  
Svetlana Goryunova ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Large Scale ◽  
Fatty Acid Content ◽  
Substantial Contribution ◽  
Oilseed Crop ◽  
Oil Composition

Abstract Background: Sunflower is an important oilseed crop domesticated in North America approximately 4000 years ago. During the last century, oil content in sunflower was under strong selection. Further improvement of the oil properties achieved by modulating its fatty acid composition is one of the main directions in modern oilseed crop breeding. Results: We searched for the genetic basis of fatty acid content variation by genotyping 601 inbred sunflower lines and assessing their lipid and fatty acid composition. Our genome-wide association analysis based on the genotypes of 15,483 SNPs and the concentrations of 23 fatty acids, including minor fatty acids, revealed significant genetic associations for eleven of them. Identified genomic regions included novel loci on chromosomes 3 and 14 cumulatively, explaining up to 34.5% of the total variation of docosanoic acid (22:0) in sunflower oil.Conclusions: This is the first large scale implementation of high-throughput lipidomic profiling on sunflower germplasm characterization. This study contributes to the genetic characterization of Russian collections, which made a substantial contribution to the development of a sunflower as the oilseed crop worldwide and provides new insights into the genetic control of oil composition that can be implemented in future studies.

scholarly journals Genetic diversity of SAD and FAD genes responsible for the fatty acid composition in flax cultivars and lines

2020 ◽  
Vol 20 (S1) ◽  
Author(s):  
Alexey A. Dmitriev ◽  
Parfait Kezimana ◽  
Tatiana A. Rozhmina ◽  
Alexander A. Zhuchenko ◽  
Liubov V. Povkhova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Genetic Polymorphism ◽  
Acid Composition ◽  
Stop Codon ◽  
Oil Composition ◽  
Illumina Platform ◽  
Flax Oil

Abstract Background Flax (Linum usitatissimum L.) is grown for fiber and seed in many countries. Flax cultivars differ in the oil composition and, depending on the ratio of fatty acids, are used in pharmaceutical, food, or paint industries. It is known that genes of SAD (stearoyl-ACP desaturase) and FAD (fatty acid desaturase) families play a key role in the synthesis of fatty acids, and some alleles of these genes are associated with a certain composition of flax oil. However, data on genetic polymorphism of these genes are still insufficient. Results On the basis of the collection of the Institute for Flax (Torzhok, Russia), we formed a representative set of 84 cultivars and lines reflecting the diversity of fatty acid composition of flax oil. An approach for the determination of full-length sequences of SAD1, SAD2, FAD2A, FAD2B, FAD3A, and FAD3B genes using the Illumina platform was developed and deep sequencing of the 6 genes in 84 flax samples was performed on MiSeq. The obtained high coverage (about 400x on average) enabled accurate assessment of polymorphisms in SAD1, SAD2, FAD2A, FAD2B, FAD3A, and FAD3B genes and evaluation of cultivar/line heterogeneity. The highest level of genetic diversity was observed for FAD3A and FAD3B genes – 91 and 62 polymorphisms respectively. Correlation analysis revealed associations between particular variants in SAD and FAD genes and predominantly those fatty acids whose conversion they catalyze: SAD – stearic and oleic acids, FAD2 – oleic and linoleic acids, FAD3 – linoleic and linolenic acids. All except one low-linolenic flax cultivars/lines contained both the substitution of tryptophan to stop codon in the FAD3A gene and histidine to tyrosine substitution in the FAD3B gene, while samples with only one of these polymorphisms had medium content of linolenic acid and cultivars/lines without them were high-linolenic. Conclusions Genetic polymorphism of SAD and FAD genes was evaluated in the collection of flax cultivars and lines with diverse oil composition, and associations between particular polymorphisms and the ratio of fatty acids were revealed. The achieved results are the basis for the development of marker-assisted selection and DNA-based certification of flax cultivars.

scholarly journals Fatty acid composition of milk thistle seed oil obtained with cold pressing

2021 ◽  
Vol 82 (4) ◽  
pp. 102-106
Author(s):  
N. L. Kleymenova
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Seed Oil ◽  
Biologically Active ◽  
Composition Analysis ◽  
Milk Thistle ◽  
Oilseed Crop ◽  
Cold Pressing

Milk thistle seeds are a unique source of biologically active substances. The oilseed crop studied is used in various fields, both for food purposes, animal husbandry and in medicine. The characteristics of milk thistle oil were considered in the work. Milk thistle seeds are known to contain 35% vegetable oil (seed fat). The oil was obtained with cold pressing. Milk thistle seed oil was chosen as the object of study. Using an experimental extruder, the oil was obtained under the following modes: the annular gap of the grain chamber - 1.25 mm, the screw rotation speed - 190 rpm, the pressing temperature - 353 K. A comparative study of the fatty acid composition of milk thistle oil components with literary sources was carried out. Gas-liquid chromatography on a Chromotek 5000 device was used to determine the composition of the components according to the GOST 31665-2012 method. The calculation for the components of milk thistle oil  on the basis of which the chromatograms were obtained for the amount of fatty acids was made. It was found out that the characteristics of the test sample are comparable with the literature data, but they also have differences, since other fatty acids were found in the oil. 24 fatty acids were identified by gas chromatographic analysis. The following ratio of fatty acids was determined: linoleic - 53%, oleic - 26%, palmitic - 8%, stearic (5%), arachidic (3%) and behenic (2%). The sample under study contains saturated and unsaturated fatty acids. According to the results of the vitamin composition analysis  milk thistle oil contains vitamins A, E and K and minor β-carotene traces which are a valuable source for diet and preventive nutrition.

scholarly journals Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Alina I. Chernova ◽  
Rim F. Gubaev ◽  
Anupam Singh ◽  
Katrina Sherbina ◽  
Svetlana V. Goryunova ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Large Scale ◽  
Fatty Acid Content ◽  
Substantial Contribution ◽  
Oilseed Crop ◽  
Oil Composition

Abstract Background Sunflower is an important oilseed crop domesticated in North America approximately 4000 years ago. During the last century, oil content in sunflower was under strong selection. Further improvement of oil properties achieved by modulating its fatty acid composition is one of the main directions in modern oilseed crop breeding. Results We searched for the genetic basis of fatty acid content variation by genotyping 601 inbred sunflower lines and assessing their lipid and fatty acid composition. Our genome-wide association analysis based on the genotypes for 15,483 SNPs and the concentrations of 23 fatty acids, including minor fatty acids, revealed significant genetic associations for eleven of them. Identified genomic regions included the loci involved in rare fatty acids variation on chromosomes 3 and 14, explaining up to 34.5% of the total variation of docosanoic acid (22:0) in sunflower oil. Conclusions This is the first large scale implementation of high-throughput lipidomic profiling to sunflower germplasm characterization. This study contributes to the genetic characterization of Russian sunflower collections, which made a substantial contribution to the development of sunflower as the oilseed crop worldwide, and provides new insights into the genetic control of oil composition that can be implemented in future studies.

scholarly journals A comparative study on quality parameters of pumpkin, melon and sunflower oils during thermal treatment

OCL ◽  
2019 ◽  
Vol 26 ◽  
pp. 32 ◽  
Author(s):  
Zhana Petkova ◽  
Ginka Antova
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Thermal Treatment ◽  
Microwave Heating ◽  
Acid Composition ◽  
Seed Oil ◽  
Conventional Heating ◽  
Melon Seed ◽  
Sunflower Oils ◽  
Melon Seed Oil

Current paper reveals the impact of thermal treatment on the quality of two seed oils – pumpkin and melon compared to the quality of the most used oil – sunflower oil. Conventional and microwave heating were used for processing the oils. The duration of the thermal treatment was 9, 12 and 18 min for the conventional heating. The microwave heating was performed with two microwave powers of the equipment (600 W and 900 W) for 3, 6, 9 and 12 min. At every stage of the thermal processing were determined acid and peroxide value, the absorbance of the oils at 232 and 268 nm, tocopherol and fatty acid composition. It was observed that the degree of oxidation of the examined oils during microwave and conventional heating increased with the duration of the thermal process and the power of the microwaves. Also, the two methods of heating had a little impact on the processes leading to the formation of free fatty acids. Total tocopherols of the melon seed oil were more stable to thermal treatment. The amount of linoleic acid decreased in the pumpkin and sunflower oils during microwave treatment, while that of oleic and palmitic acid relatively increased. The biggest change in the fatty acid composition of both oils was found during microwave heating at 900W. The changes in fatty acid composition of thermally treated melon seed oil were insignificant. Overall, melon seed oil was observed to be more thermally stable than pumpkin and sunflower oils.

Change in plant densities combined with zinc application affects rapeseed seed oil and fatty acid composition

2021 ◽  
pp. 1-11
Author(s):  
Mohammad Agha Mohammad Reza ◽  
Farzad Paknejad ◽  
Amir Hossein Shirani Rad ◽  
Mohammad Reza Ardakani ◽  
Ali Kashani
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Seed Oil ◽  
Plant Densities ◽  
Zinc Application

scholarly journals Effect of Nitrogen Fertilisation and Inoculation with Bradyrhizobium japonicum on the Fatty Acid Profile of Soybean (Glycine max (L.) Merrill) Seeds

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 941
Author(s):  
Ewa Szpunar-Krok ◽  
Anna Wondołowska-Grabowska ◽  
Dorota Bobrecka-Jamro ◽  
Marta Jańczak-Pieniążek ◽  
Andrzej Kotecki ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Bradyrhizobium Japonicum ◽  
Field Experiments ◽  
Fatty Acid Content ◽  
Soybean Seeds ◽  
Oilseed Crop ◽  
Soybean Cultivars

Soybean is a valuable protein and oilseed crop ranked among the most significant of the major crops. Field experiments were carried out in 2016–2019 in South-East Poland. The influence of soybean cultivars (Aldana, Annushka), nitrogen fertilizer (0, 30, 60 kg∙ha−1 N) and inoculation with B. japonicum (control, HiStick® Soy, Nitragina) on the content of fatty acids (FA) in soybean seeds was investigated in a three-factorial experiment. This study confirms the genetic determinants of fatty acid composition in soybean seeds and their differential accumulation levels for C16:0, C16:1, C18:1n9, C18:2, C18:3, and C20:0 as well saturated (SFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids. Increasing the rate from 30 to 60 kg ha−1 N did not produce the expected changes, suggesting the use of only a “starter” rate of 30 kg ha−1 N. Inoculation of soybean seeds with a strain of Bradyrhizobium japonicum (HiStick® Soy, BASF, Littlehampton, UK and Nitragina, Institute of Soil Science and Plant Cultivation–State Research Institute, Puławy, Poland) is recommended as it will cause a decrease in SFA and C16:0 acid levels. This is considered nutritionally beneficial as its contribution to total fatty acids determines the hypercholesterolemic index, and it is the third most accumulated fatty acid in soybean seeds. The interaction of cultivars and inoculation formulation on fatty acid content of soybean seeds was demonstrated. An increase in the value of C16:0 content resulted in a decrease in the accumulation of C18:1, C18:2, and C18:3 acids. The content of each decreased by almost one unit for every 1% increase in C16:0 content. The dominant effect of weather conditions on the FA profile and C18:2n6/C18:3n3 ratio was demonstrated. This suggests a need for further evaluation of the genetic progress of soybean cultivars with respect to fatty acid composition and content under varying habitat conditions.

Fatty acid composition of alfalfa seed oil

1996 ◽  
Vol 31 (4) ◽  
pp. 518-518
Author(s):  
A. I. Rezvukin ◽  
I. Yu Berezovikova ◽  
I. Ya Shalaurova ◽  
Yu. P. Nikitin
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Seed Oil ◽  
Alfalfa Seed
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