scholarly journals Quantitative trait loci (QTL) analysis of leaf related traits in spinach (Spinacia oleracea L.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhiyuan Liu ◽  
Hongbing She ◽  
Zhaosheng Xu ◽  
Helong Zhang ◽  
Guoliang Li ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Spinacia Oleracea ◽  
Snp Markers ◽  
Leaf Length ◽  
Chromosome 1 ◽  
Leaf Width ◽  
Linkage Maps ◽  
Petiole Length ◽  
Phosphodiester Bond ◽  
Major Qtls

Abstract Background Spinach (Spinacia oleracea L.) is an important leafy vegetable crop, and leaf-related traits including leaf length, leaf width, and petiole length, are important commercial traits. However, the underlying genes remain unclear. The objective of the study was to conduct QTL mapping of leaf-related traits in spinach. Results A BC1 population was used to construct the linkage map and for QTL mapping of leaf length, leaf width, petiole length, and the ratio of leaf length to width in 2015 and 2019. Two genetic linkage maps were constructed by specific locus amplified fragment sequencing (SLAF-seq), and kompetitive allele specific PCR (KASP) technology, respectively using BC1 population in 2015. Based on the results of 2015, the specific linkage groups (LG) detected QTLs were generated using BC1 population in 2019. A total of 13 QTLs were detected for leaf-related traits, only five QTLs being repeatedly detected in multiple years or linkage maps. Interestingly, the major QTLs of leaf length, petiole length, and the ratio of leaf length to width were highly associated with the same SNP markers (KM3102838, KM1360385 and KM2191098). A major QTL of leaf width was mapped on chromosome 1 from 41.470−42.045 Mb. And 44 genes were identified within the region. Based on the GO analysis, these genes were significantly enriched on ribonuclease, lyase activity, phosphodiester bond hydrolysis process, and cell wall component, thus it might change cell size to determine leaves shape. Conclusions Five QTLs for leaf-related traits were repeatedly detected at least two years or linkage maps. The major QTLs of leaf length, petiole length, and the ratio of leaf length to width were mapped on the same loci. And three genes (Spo10792, Spo21018, and Spo21019) were identified as important candidate genes for leaf width.

scholarly journals Biplot analysis of trait relations of spinach (Spinacia oleracea L.) landraces

Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 675-690
Author(s):  
Naser Sabaghnia ◽  
Mehdi Mohebodini ◽  
Mohsen Janmohammadi
Keyword(s):  
Spinacia Oleracea ◽  
Block Design ◽  
Dry Weight ◽  
Leaf Length ◽  
First Year ◽  
Vegetable Crops ◽  
Petiole Length ◽  
Spinacia Oleracea L ◽  
Leaf Yield ◽  
Spinach Leaf

Interest in growing winter spinach (Spinacia oleracea L.) in Iran is increasing due to its good nutritional potential returns relative to other vegetable crops. The objectives of this research were to investigate the interrelationships among different traits of spinach and to evaluate different Iranian spinach landraces with application of the genotype ? trait (GT) biplot methodology in visualizing research data. 81 spinach landraces were grown during 2-years according to randomized complete block design with four replications. Ranking of the genotypes based on the ideal entry revealed that genotypes G1, G20, G7, G8, G9, G27, G49 G70 and G79 were higher in the measured traits and could be good candidates for improving most of the measured traits. Ranking of traits for the leaf yield showed that petiole diameter, petiole length, leaf numbers at flowering, 1000-seed weight and root dry weight were the most discriminating traits which influence spinach leaf yield at both years. There were 9 winning genotypes and 4 which-won-where patterns at the first year while there were 8 winning genotypes and 4 which-won-where patterns at the second year. As a result, the findings from our study are as follows: (i) traits leaf numbers at flowering, leaf length, leaf width, leaf area, petiole diameter and petiole length could be as selection indices for spinach leaf yield improvement, (ii) genotypes G1, G20, G7, G8, G9, G27, G49 G70, and G79 were the most favorable and is thus recommended for commercial release or incorporating in breeding programs; (iii) the GT biplot method can be used to identify superior genotypes in other crops and in other parts of the world.

scholarly journals Genetic variability and correlation studies of mulberry (Morus alba L.) genotypes in Bangladesh

2020 ◽  
Vol 49 (3) ◽  
pp. 685-691
Author(s):  
Md Serajur Rahman ◽  
SM Shahinul Islam
Keyword(s):  
Growth Traits ◽  
Environmental Influence ◽  
Leaf Length ◽  
Leaf Width ◽  
Genetic Advance ◽  
Fresh Weight ◽  
Petiole Length ◽  
Branch Number ◽  
Leaf Yield ◽  
Shoot Weight

Morphological, phenotypical and yield attributing characteristics of 20 mulberry genotypes were evaluated. Genotypic and phenotypic variations, heritability, genetic advance and correlation co-efficient were also estimated. It was found that the phenotypic co-efficient of variation (PCV) was higher (97.68%) than genotypic co-efficient of variation (GCV, 96.99%). The broad sense heritability for these traits ranged from 98.60 (AL) to 4.69 (LLS). High heritability coupled with high genetic advance was recorded for the characters apex length (AL), leaf length (LL), leaf width (LW), leaf petiole ratio (LPR) and petiole length (PL) suggesting the higher genetic control over these traits. Significant positive correlations to leaf yield/plant were observed for the characters, namely total shoot weight (0.817), longest shoot length (0.600), total branch height (0.596) and leaves fresh weight/10 leaves (0.425). Leaf yield showed significantly positive phenotypic and genotypic correlations with all other growth traits (viz., total shoot weight 0.817, length of longest shoot 0.600, total branch height 0.596 and leaves fresh weight/10 leaves 0.425) except total branch number, nodes per meter, leaf width and petiole length. High genetic advance as percentage of mean coupled with heritability was observed on AL, LL, LW, LPR, PL and 10 fresh leaves weight suggesting the prevalence of additive gene action with low environmental influence for the determination of these characters and could be effective in phenotypic selection. Analysis of variances (ANOVA) for characters such as AL, LL, LW, LPR and PL showed significant variations among the genotypes. Since mulberry is mainly cultivated for leaf yield, genotypes having higher AL, LL, LW and LPR and PL must be given importance during parent selection to evolve high yielding varieties across different seasons in mulberry.

scholarly journals A Study of Morphophysiological Descriptors of Cultivated Anthurium andraeanum Hort.

HortScience ◽  
2012 ◽  
Vol 47 (9) ◽  
pp. 1234-1240
Author(s):  
Winston Elibox ◽  
Pathmanathan Umaharan
Keyword(s):  
Principal Component ◽  
Leaf Size ◽  
Leaf Length ◽  
Leaf Width ◽  
Petiole Length ◽  
Frequency Distributions ◽  
Anthurium Andraeanum ◽  
Peduncle Length ◽  
The Right ◽  
Leaf Parameters

Sixteen morphophysiological parameters of horticultural importance were investigated in 82 anthurium accessions grown in the Caribbean. The spathe colors included red, pink, white, green, orange, purple, coral, and brown and obake types with red, pink, and white spathe colors accounting for 63.4% of the accessions. There was wider variation in spadix color combinations than spathe color. There was wide variation for the cut flower and leaf parameters evaluated with productivity and peduncle length having the smallest and largest range, respectively. The frequency distributions were skewed to the right for spathe length, spathe width, spathe size, spadix length, spadix diameter, leaf width, leaf size, and productivity and was normal for leaf length, spadix angle, peduncle length, petiole length, and spathe showiness. Accessions with wider leaves had longer leaves and longer petioles; those with longer spathes had wider spathes; and those with longer peduncles had correspondingly longer petioles. Peduncle length also showed moderate, weak correlations with other leaf measurements and spathe parameters, respectively. Spadix diameter showed moderate correlations with leaf parameters. Spathe showiness showed moderate, weak correlations with spathe measurements and productivity, respectively. Principal component analysis of the 13 quantitative parameters showed that the first three principal components explained 75% of the variation in the accessions. Leaf length, leaf width, leaf size, petiole length, peduncle length, and spadix diameter; spathe length, spathe width, spathe size, and spathe showiness; and productivity loaded on the first, second, and third component, respectively. Hence, one leaf and one spathe size parameter, spathe showiness, petiole length, peduncle length, spadix length, spadix angle, spadix diameter, and productivity as well as spathe, spadix, peduncle, and petiole colors can be used as descriptors for anthurium. Based on these results and market information, an Anthurium ideotype adapted to the humid tropics is described.

scholarly journals Terede (Lepidium Sativum L.) Bitki Sıklığının Verim ve Yaprak Kalitesine Etkisi

2019 ◽  
Vol 7 (8) ◽  
pp. 1222
Author(s):  
Dilek Yılmaz ◽  
Atnan Uğur
Keyword(s):  
Chlorophyll Content ◽  
Leaf Length ◽  
Leaf Width ◽  
Garden Cress ◽  
Petiole Length ◽  
Plant Materials ◽  
Leaf Quality ◽  
Plant Yield ◽  
Leaf Petiole ◽  
Plant Densities

This study was carried out in greenhouse and laboratories of Department of Horticulture (Faculty of Agriculture, Ordu University, Ordu, Turkey) in 2013-2014 production seasons in Ordu ecological condition. Commercial garden cress standard seeds were used as plant materials. Plant densities were calculated upon the seed quantity spread on 1 m2, and seeds were sown for 1.0, 1.5, 2.0 and 2.5 g per m2. Peat: perlite mix prepared in proportion of 3:1 was used as the growing medium and filled in 50×16×14 cm sized plastic pots. The garden cress plants were harvested on the 45th day after seeding. The yield, leaf petiole length, leaf length, leaf width and chlorophyll content of the harvested plants were determined. It has been determined that quality parameters vary depending on plant density and varieties. The highest plant yield was obtained from “BT Bu-ter” garden cress variety with 2.489.2 g/m2. The plant yield in different plant densities was increased in the rates varying between 3.31% and 8.25%. The increasing plant densities caused an increase in yield but negatively affected the leaf quality in terms of both length and chlorophyll content. Leaf width, leaf length, leaf petiole length and chlorophyll content were decreased depending on the increase in plant densities. Based on the information obtained, it was considered useful to choose a plant densities based on the variety and growing season according to the growing purpose.

scholarly journals Genetic Variability Analysis in Local Germplasm of Ivy Gourd (Coccinia grandis L.) in Southern Rajasthan Conditions

2020 ◽  
pp. 104-111
Author(s):  
Jitendra Kumar Tak ◽  
Shalini Pilania ◽  
Ram Avtar Kaushik ◽  
S. S. Lakhawat ◽  
Mithlesh Kumari Meena ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Good Deal ◽  
Fruit Weight ◽  
Leaf Length ◽  
Leaf Width ◽  
Petiole Length ◽  
College Of Agriculture ◽  
Fruit Length ◽  
Local Germplasm ◽  
Ivy Gourd

An experiment was carried out on study of genetic variability in 30 genotypes of ivy gourd at Horticulture Department of Rajasthan College of Agriculture, MPUAT, Udaipur during July to September 2017. Twelve Growth and quality characters were studied. The analysis of variance indicated that the mean sum of square due to genotypes were highly significant for all the characters suggesting the presence of good deal of variability in material studied. High magnitude of genotypic as well as phenotypic coefficient of variations were recorded for traits viz., petiole length (27.63% and 29.93%), inter nodal length (22.73% and 23.40%), leaf width (22.63% and 23.35%) and leaf width (22.52% and 23.56%) and highest heritability recorded for inter nodal length (94.30%) followed by leaf length (93.92%), leaf width (91.37%), ascorbic acid (89.48 %), petiole length (85.17%), fruit weight (82.10%), fruit length (79.98%) and chlorophyll content in leaves (78.23%).The highest genetic gain was recorded for petiole length (52.52%) followed by viz., inter nodal length (45.47%), leaf length (45.17%), leaf width (44.34%), fruit weight (34.21%),  fruit length (28.24%) fruit diameter (23.31%) & genetic advance were also higher for some characters. Availability of high GCV & PCV shows presence of sufficient genetic variability for evaluated traits. This indicates that there is an ample scope of selection in the present gene pool for yield and its components.

scholarly journals High resolution mapping and candidate gene identification of downy mildew race 16 resistance in spinach

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Gehendra Bhattarai ◽  
Wei Yang ◽  
Ainong Shi ◽  
Chunda Feng ◽  
Braham Dhillon ◽  
...  
Keyword(s):  
Candidate Genes ◽  
Downy Mildew ◽  
Association Analysis ◽  
Spinacia Oleracea ◽  
Genotyping By Sequencing ◽  
Significant Snps ◽  
Snp Markers ◽  
Resistance Locus ◽  
Downy Mildew Resistance ◽  
Mildew Resistance

Abstract Background Downy mildew, the most devastating disease of spinach (Spinacia oleracea L.), is caused by the oomycete Peronospora effusa [=P. farinosa f. sp. spinaciae]. The P. effusa shows race specificities to the resistant host and comprises 19 reported races and many novel isolates. Sixteen new P. effusa races were identified during the past three decades, and the new pathogen races are continually overcoming the genetic resistances used in commercial cultivars. A spinach breeding population derived from the cross between cultivars Whale and Lazio was inoculated with P. effusa race 16 in an environment-controlled facility; disease response was recorded and genotyped using genotyping by sequencing (GBS). The main objective of this study was to identify resistance-associated single nucleotide polymorphism (SNP) markers from the cultivar Whale against the P. effusa race 16. Results Association analysis conducted using GBS markers identified six significant SNPs (S3_658,306, S3_692697, S3_1050601, S3_1227787, S3_1227802, S3_1231197). The downy mildew resistance locus from cultivar Whale was mapped to a 0.57 Mb region on chromosome 3, including four disease resistance candidate genes (Spo12736, Spo12784, Spo12908, and Spo12821) within 2.69–11.28 Kb of the peak SNP. Conclusions Genomewide association analysis approach was used to map the P. effusa race 16 resistance loci and identify associated SNP markers and the candidate genes. The results from this study could be valuable in understanding the genetic basis of downy mildew resistance, and the SNP marker will be useful in spinach breeding to select resistant lines.

scholarly journals Do we have infraspecific taxa of Salvia multicaulis Vahl. (Lamiaceae) in Iran?

2017 ◽  
Vol 7 (4) ◽  
pp. 432-439 ◽  
Author(s):  
Seyed Mehdi Talebi ◽  
Reza Rezakhanlou ◽  
A V. Matsyura
Keyword(s):  
Leaf Shape ◽  
Morphological Characteristics ◽  
Leaf Length ◽  
Width Ratio ◽  
Petiole Length ◽  
Widespread Species ◽  
Basal Leaf ◽  
Leaf Petiole ◽  
Upgma Cluster Analysis ◽  
Length Width

<p><em>Salvia multicaulis</em> is a widespread species of Lamiaceae family in Iran. There are many discussions about its infraspecific variations. Although some varieties were definite for this species in various parts of the world, no infraspecific taxon was reported in Iran and all samples of this species were named as S. multicaulis. In this study, morphological characteristics of S. multicaulis populations, naturally growing in Iran, was examined. Twenty-two traits were examined in 94 individuals of this species to<br />identify their phenotypic difference. Most of the investigated features were showing a high degree of variability, but it was highly pronounced for some characteristics such as basal leaf shape, basal leaf width, basal leaf length/ width ratio and basal petiole length. Significant positive/negative correlations were observed between some morphological variables. Furthermore, significant negative correlations were found between the eastern distribution of populations with basal leaf petiole length and basal leaf length/ width ratio. Based on the UPGMA cluster analysis, populations were divided into two main branches. The first branch contained four populations, while the second branch was bigger and clustered in two sub-branches. In one of them,<br />three populations and in another one the rest populations arranged in two groups. CA joined plot confirmed that each of studied populations or group of populations had distinct morphological trait(s), which were useful in identification of them. Our findings supported population no. 13 had unique morphological traits such as the largest bracts and basal leaf petiole, highest flower number of each inflorescence cycle, widest and largest calyx. The conservation of the highly diverse populations of<br />Iranian S. multicaulis is recommended.</p>

scholarly journals Pertumbuhan Vegetatif dan Kadar Gula Biji Jagung Manis (Zea mays saccharata, Sturt) di Pekanbaru

2018 ◽  
Vol 13 (2) ◽  
pp. 73-78 ◽  
Author(s):  
Surtinah Surtinah ◽  
Seprita Lidar
Keyword(s):  
Plant Height ◽  
Sweet Corn ◽  
Polynomial Regression ◽  
Sugar Content ◽  
Leaf Length ◽  
Leaf Width ◽  
Design Environment ◽  
Corn Seed ◽  
Randomized Design ◽  
Corn Kernels

Research conducted an experiment using a completely randomized design environment with four replications, and the design of treatment used is six varieties of sweet corn. Analysis of data using polynomial regression, the parameters measured were plant height, leaf number, leaf length, leaf width, and sugar beans, followed by analyzing the relationship between the growth of plants with a sugar content of sweet corn kernels. The results showed that leaf width gives a weak relationship to the sugar content of sweet corn seed, and leaf length, number of leaves and plant height had a close relationship to the sugar content of sweet corn kernels.

scholarly journals STIMULASI HASIL MELON ( Cucumis melo, L) DENGAN MENGGUNAKAN BIOTO GROW GOLD (BGG)

2018 ◽  
Vol 15 (1) ◽  
pp. 35-41
Author(s):  
SAHINDAH ARITONANG ◽  
SURTINAH SURTINAH
Keyword(s):  
Data Analysis ◽  
Cucumis Melo ◽  
Sugar Content ◽  
Fruit Weight ◽  
Leaf Length ◽  
Stem Diameter ◽  
Leaf Width ◽  
Randomized Design ◽  
Cucumis Melo L ◽  
Melon Fruit

The experiment was conducted experimentally using non-factorial Randomized Design (RAL) with 5 (five) treatment levels without Bioto Grow Gold, Bioto Grow Gold 1 ml liter-1 water, Bioto Grow Gold 2 ml liter -1 water, giving Bioto Grow Gold 3 ml liter-1 water and giving Bioto Grow Gold 4 ml liter-1 water. The data analysis used variance and continued with a different test of Duncan treatment average at p 0.05. The results showed that BGG treatment had the significant effect on leaf length, leaf width, stem diameter, flowering age, fruit circumference, fruit weight, a thickness of flesh and sugar content of melon fruit. The best treatment is giving Bioto Grow Gold 3 ml liter-1 water.

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