Effects of Saccharomyces cerevisiae cell wall addition on feed digestibility, fecal fermentation and microbiota and immunological parameters in adult cats

Abstract Background This study aimed to evaluate the effects of increasing dosages of a commercial product composed by Saccharomyces cerevisiae yeast (YAM), with active metabolites, which are beta glucans, nucleotides, organic acids, polyphenols, amino acids, vitamins and minerals (Original XPCtm, Diamond V, IOWA, USA) added to a commercially available dry cat food. Apparent digestibility of dietary nutrients, fecal microbiota, fecal fermentation products and immunological parameters were evaluated. Twenty-seven healthy cats of mixed sexes, with a mean body weight of 4.19 ± 0.83 kg and a mean age of 9.44 ± 5.35 years were distributed by age in an unbalanced randomized block design, consisting of three experimental treatments: CD (control diet), YAM 0.3 (control diet with 0.3% yeast with active metabolites) and YAM 0.6 (control diet with 0.6% yeast with active metabolites). Results The inclusion of the additive elevated the apparent digestibility of crude fiber (p = 0.013) and ash (p < 0.001) without interfering feed consumption, fecal production and fecal characteristics. Regarding fermentation products present in the feces, prebiotic inclusion increased lactic acid concentration (p = 0.004) while reducing isovaleric acid (p = 0.014), only in the treatment YAM 0.3. No differences were noticed on biogenic amines (BA), fecal pH, ammonia concentration, total and individuals short-chain fatty acids (SCFA) and total and individuals branched-chain fatty acids (BCFA) (except isovaleric acid in YAM 0.3). As regards to fecal microbiota, prebiotic inclusion has resulted in the reduction of Clostridium perfringens (p = 0.023). No differences were found in the immunological parameters evaluated. Conclusion It can be concluded that the additive, at the levels of inclusion assessed shows prebiotic potential and it has effects on fecal fermentation products and microbiota without interfering on crude protein and dry matter digestibility. More studies evaluating grater inclusion levels of the prebiotic are necessary to determine optimal concentration.

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Saccharomyces cerevisiae Dehydrated Culture Modulates Fecal Microbiota and Improves Innate Immunity of Adult Dogs

Fermentation ◽

10.3390/fermentation8010002 ◽

2021 ◽

Vol 8 (1) ◽

pp. 2

Author(s):

Karine de Melo Santos ◽

Larissa Wünsche Risolia ◽

Mariana Fragoso Rentas ◽

Andressa Rodrigues Amaral ◽

Roberta Bueno Ayres Rodrigues ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Healthy Adult ◽

Control Diet ◽

Fecal Microbiota ◽

Nutrient Digestibility ◽

Yeast Culture ◽

Immunological Parameters ◽

Protein Nitrogen ◽

Fermentation Products ◽

Inclusion Level

Saccharomyces cerevisiae yeast culture can be dehydrated, and it has a potential prebiotic effect. This study evaluated the effects of supplementing increasing levels of dehydrated yeast culture (DYC) of Saccharomyces cerevisiae (Original XPC™, Diamond V, Cedar Rapids, IA, USA) on fecal microbiota, nutrient digestibility, and fermentative and immunological parameters of healthy adult dogs. Eighteen adult male and female dogs with a mean body weight of 15.8 ± 7.37 kg were randomly assigned to three experimental treatments: CD (control diet), DYC 0.3 (control diet with 0.3% DYC) and DYC 0.6 (control diet with 0.6% DYC). After 21 days of acclimation, fecal samples were collected for analysis of nutrient digestibility, microbiota and fecal fermentation products. On the last day, the blood samples were collected for the analysis of immunological parameters. The microbiome profile was assessed by the Illumina sequencing method, which allowed identifying the population of each bacterial phylum and genus. The statistical analyses were performed using the SAS software and the Tukey test for multiple comparison (p < 0.05). Our results suggest that the addition of DYC increased the percentage of the phyla Actinobacteria and Firmicutes (p = 0.0048 and p < 0.0001, respectively) and reduced that of the phylum Fusobacteria (p = 0.0008). Regardless of the inclusion level, the yeast addition promoted reduction of the genera Allobaculum and Fusobacterium (p = 0.0265 and p = 0.0006, respectively) and increased (p = 0.0059) that of the genus Clostridium. At the highest prebiotic inclusion level (DYC 0.6), an increase (p = 0.0052) in the genus Collinsella and decrease (p = 0.0003) in Prevotella were observed. Besides that, the inclusion of the additive improved the apparent digestibility of the crude fiber and decreased the digestibility of crude protein, nitrogen-free extract and metabolizable energy (p < 0.05). There were no significant changes in the production of volatile organic compounds. However, an increase in propionate production was observed (p = 0.05). In addition, the inclusion of yeast resulted in an increased phagocytosis index in both treatments (p = 0.01). The addition of 0.3 and 0.6% DYC to the diet of dogs wase able to modulate the proportions of some phyla and genera in healthy dogs, in addition to yielding changes in nutrient digestibility, fermentative products and immunity in healthy adult dogs, indicating that this additive can modulate fecal microbiota and be included in dog nutrition.

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PSIII-13 Effects of Saccharomyces Cerevisiae Fermentation Products on Lactating Sow Performance

Journal of Animal Science ◽

10.1093/jas/skab054.290 ◽

2021 ◽

Vol 99 (Supplement_1) ◽

pp. 172-172

Author(s):

Ricardo M Garcia ◽

Morgan T Thayer ◽

Kayla M Mills ◽

Jacob A Richert ◽

Katharine G Sharp ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Water Intake ◽

Feed Intake ◽

Control Diet ◽

Feed Additives ◽

Intermediate Water ◽

Lactation Period ◽

Fermentation Products ◽

Once Daily ◽

Lactating Sows

Abstract Lactating sows (N=140, York x Landrace) were used to evaluate the effects of a liquid prototype (LIQP) and dry (XPC®; Diamond V) Saccharomyces cerevisiae feed additives on sow and litter performance. Sows were fed a common gestation diet (0.55% SID-Lysine) until d112 of pregnancy and then allotted to lactation treatments: 1) Control diet (CON; 1.00% SID-Lysine), 2) CON +15 mL of LIQP from d112 to weaning (LIQ), 3) CON +0.20% of XPC from d112 to weaning (DRY), and 4) DRY +15 mL of LIQP from d112 to d7 post-farrowing (D+L). The LIQP was given once daily using an oral dose gun and XPC was included in the feed. Immunoglobulin concentrations were estimated on colostrum samples using Brix refractometer and piglet d 1 serum immunocrit ratio and plasma IgA and IgG. Daily sow water intake and daily feed intake (DFI) from d112 of gestation to d7 post-farrowing and weekly feed intake (ADFI) were recorded. There were no treatment effects on sow BW, backfat, or loin depth (P >0.05) although sows from LIQ group had numerically smaller BW reductions compared to CON sows (4.9% vs 7.2%, P=0.19). LIQ sows had greater DFI and CON lower DFI during week 1 of lactation (P=0.04) as well as ADFI for weeks 2, 3, and overall lactation period (P< 0.01) with DRY and D+L sows being intermediate. Water intake, immunoglobulins, and litter performance did not differ among treatments (P >0.05) although pigs from LIQ sows, compared to CON, had numerically increased weaning (6.14 vs 5.82kg, P >0.05) and litter weights (63.2 vs 60.1kg, P >0.05). LIQ sows had 0.7 d reduced wean-to-estrus interval (P< 0.001) and tended to have greater conception rates (P=0.07). In conclusion, LIQ supplementation of lactating sows improves feed intake, allowing sows to keep body reserves, and have better subsequent rebreeding performance.

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The absorption of stearic acid from triacylglycerols: an inquiry and analysis

Nutrition Research Reviews ◽

10.1079/095442200108729061 ◽

2000 ◽

Vol 13 (2) ◽

pp. 185-214 ◽

Cited By ~ 26

Author(s):

Geoffrey Livesey

Keyword(s):

Fatty Acids ◽

Body Weight ◽

Melting Point ◽

Cocoa Butter ◽

Stearic Acid ◽

Short Chain ◽

Apparent Digestibility ◽

Long Chain Fatty Acids ◽

Long Chain ◽

Chain Fatty Acids

AbstractAlthough stearic acid is a saturated fatty acid, its influence on plasma cholesterol acid other health variables is neutral; possibly owing in part to poor absorption. Reduced absorption of stearic acid from particular triacylglycerols, cocoa butter and novel fats formulated with short- and long-chain acid triacylglycerol molecules (Salatrims) has been attributed to high intakes. However, the circumstances and causes of poor stearic acid digestion from triacylglycerols are unclear; published data were therefore collected and analysed, with emphasis on human studies. Of twenty-eight studies conducted in adults, most are in men (>90%). The assertion that reduced absorption is due to a high intake of stearoyl groups is not supported: dietary intakes of stearoyl of 0·05–0·65 g stearic acid equivalent/kg body weight (cf typical intake of 0·2 g stearic acid equivalent/kg body weight in the Western diet) indicate that the ‘true’ digestibility of stearoyl is 0·98 (SE 0·01) g/g, with apparent digestibility less than this value at low intakes owing to endogenous stearic acid excretion and to inter-publication variation of unidentified cause. The neutral health impact of stearic acid must be due to factors other than availability. Exceptions include cocoa butter, Salatrims and tristearin, for which digestibility is an additional factor. The efficiency with which human subjects digest stearoyl from cocoa butter still remains uncertain, while the digestion of total long-chain fat from this source is 0·89–0·95 g/g, high in comparison with 0·33 g/g for Salatrim 23CA and 0·15 g/g for tristearin in their prepared states. Salatrims contain the highest proportion of long-chain fatty acids that are stearic acid-rich other than tristearin, which is the main component of fully-hydrogenated soyabean and rapeseed oil. Analysis shows that apparent digestibility of stearic acid is associated with stearoyl density within the triacylglycerol molecule and that, in Salatrims, the occurrence of short-chain fatty acids in place of long-chain fatty acids increases this density. Soap formation appears not to be a major factor in the reduced digestion of stearic acid from tristearin under regular dietary circumstances, but both microcrystallinity and reduced digestibility of tri-, di- and monostearoylglycerols appears to be important. Solubilisation of high-melting-point tristearin in low-melting-point oils improves the digestibility of its stearic acid, particularly when emulsified or liquidized at above melting point. However, without such artificial aids, the digestive tracts of the rat, dog and man have a low capacity for emulsifying and digesting stearic acid from tristearin. Reduced digestibility of stearic acid from Salatrim 23CA also appears to be attributable to reduced digestibility of di- and monostearoylglycerols and is particularly due to remnants with the 1- or 3-stearoylglycerol intact after initial hydrolytic cleavage. Short-chain organic acid in Salatrim 23CA, which is readily hydrolysed, leaves such remnants. Unlike tristearin, Salatrim 23CA melts at body temperature and mixing it with low-melting-point oils is not expected to cause further disruption of microcrystalline structures to aid digestibility of its stearoyl groups. The low digestibility of stearoyl in Salatrim 23CA, together with the occurrence of short-chain organic acids in this product, account for its relatively low nutritional energy value (about 20 kJ (5 kcal)/g) compared with traditional fats (37 kJ (9 kcal)/g) and low fat value (<20:37 kJ/kJ; <5:9 kcal/kcal) relative to traditional fats. In part these differences are because of minor effects of Salatrim 23CA on the excretion of other fat and protein, due to the bulking properties of this poorly-digestible fat.

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Validated Postbiotic Screening Confirms Presence of Physiologically-Active Metabolites, Such as Short-Chain Fatty Acids, Amino Acids and Vitamins in Hylak® Forte

Probiotics and Antimicrobial Proteins ◽

10.1007/s12602-018-9497-5 ◽

2018 ◽

Vol 11 (4) ◽

pp. 1124-1131 ◽

Cited By ~ 5

Author(s):

Satish Patil ◽

Sarvesh Sawant ◽

Karlheinz Hauff ◽

Gabriele Hampp

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Short Chain Fatty Acids ◽

Short Chain ◽

Active Metabolites ◽

Chain Fatty Acids

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Protein fermentation in the gut; implications for intestinal dysfunction in humans, pigs, and poultry

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00319.2017 ◽

2018 ◽

Vol 315 (2) ◽

pp. G159-G170 ◽

Cited By ~ 16

Author(s):

Myrthe S. Gilbert ◽

Noortje Ijssennagger ◽

Arie K. Kies ◽

Saskia W. C. van Mil

Keyword(s):

Fatty Acids ◽

Dietary Protein ◽

Protein Intake ◽

Intestinal Disease ◽

Gut Health ◽

Bacterial Strains ◽

Fermentation Products ◽

Epithelial Damage ◽

Production Animals ◽

Chain Fatty Acids

The amount of dietary protein is associated with intestinal disease in different vertebrate species. In humans, this is exemplified by the association between high-protein intake and fermentation metabolite concentrations in patients with inflammatory bowel disease. In production animals, dietary protein intake is associated with postweaning diarrhea in piglets and with the occurrence of wet litter in poultry. The underlying mechanisms by which dietary protein contributes to intestinal problems remain largely unknown. Fermentation of undigested protein in the hindgut results in formation of fermentation products including short-chain fatty acids, branched-chain fatty acids, ammonia, phenolic and indolic compounds, biogenic amines, hydrogen sulfide, and nitric oxide. Here, we review the mechanisms by which these metabolites may cause intestinal disease. Studies addressing how different metabolites induce epithelial damage rely mainly on cell culture studies and occasionally on mice or rat models. Often, contrasting results were reported. The direct relevance of such studies for human, pig, and poultry gut health is therefore questionable and does not suffice for the development of interventions to improve gut health. We discuss a roadmap to improve our understanding of gut metabolites and microbial species associated with intestinal health in humans and production animals and to determine whether these metabolite/bacterial networks cause epithelial damage. The outcomes of these studies will dictate proof-of-principle studies to eliminate specific metabolites and or bacterial strains and will provide the basis for interventions aiming to improve gut health.

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