scholarly journals Molecular surveillance of anti-malarial resistance pfcrt, pfmdr1, and pfk13 polymorphisms in African Plasmodium falciparum imported parasites to Wuhan, China

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Weijia Cheng ◽  
Xiaonan Song ◽  
Huabing Tan ◽  
Kai Wu ◽  
Jian Li
Keyword(s):  
Plasmodium Falciparum ◽  
Migrant Workers ◽  
Mixed Type ◽  
Cross Sectional Study ◽  
Imported Malaria ◽  
Wild Type ◽  
Mutant Type ◽  
Cross Sectional ◽  
Molecular Surveillance ◽  
Public Health Challenge

Abstract Background Imported malaria parasites with anti-malarial drug resistance (ADR) from Africa is a serious public health challenge in non-malarial regions, including Wuhan, China. It is crucial to assess the ADR status in African Plasmodium falciparum isolates from imported malaria cases, as this will provide valuable information for rational medication and malaria control. Methods During 2017–2019, a cross-sectional study was carried out in Wuhan, China. Peripheral blood 3 ml of returned migrant workers from Africa was collected. The target fragments from pfcrt, pfmdr1, and k13 propeller (pfk13) genes were amplified, sequenced, and analysed. Results In total, 106 samples were collected. Subsequently, 98.11% (104/106), 100% (106/106), and 86.79% (92/106) of these samples were successfully amplified and sequenced for the pfcrt (72–76), pfmdr1, and pfk13 genes, respectively. The prevalence of the pfcrt 76 T, pfmdr1 86Y, and pfmdr1 184F mutations was 9.62, 4.72, and 47.17%, respectively. At codons 72–76, the pfcrt locus displayed three haplotypes, CVMNK (wild-type), CVIET (mutation type), CV M/I N/E K/T (mixed type), with 87.50%, 9.62%, and 2.88% prevalence, respectively. For the pfmdr1 gene, NY (wild type), NF and YF (mutant type), N Y/F, Y Y/F, and N/Y Y/F (mixed type) accounted for 34.91, 43.40, 3.77, 15.09, 0.94, and 1.89% of the haplotypes, respectively. A total of 83 isolates with six unique haplotypes were found in pfcrt and pfmdr1 combined haplotypes, of which NY-CVMNK and NF-CVMNK accounted for 40.96% (34/83) and 43.37% (36/83), respectively. Furthermore, 90 cases were successfully sequenced (84.91%, 90/106) at loci 93, 97, 101, and 145, and 78 cases were successfully sequenced (73.58%, 78/106) at loci 343, 353, and 356 for pfcrt. However, the mutation was observed only in locus 356 with 6.41%. For pfk13, mutations reported in Southeast Asia (at loci 474, 476, 493, 508, 527, 533, 537, 539, 543, 553, 568, 574, 578, and 580) and Africa (at loci 550, 561, 575, 579, and 589) were not observed. Conclusions The present data from pfcrt and pfmdr1 demonstrate that anti-malarial drugs including chloroquine, amodiaquine, and mefloquine, remain effective against malaria treatment in Africa. The new mutations in pfcrt related to piperaquine resistance remain at relatively low levels. Another source of concern is the artemether-lumefantrine resistance-related profiles of N86 and 184F of pfmdr1. Although no mutation in pfk13 is detected, molecular surveillance must continue.

scholarly journals Molecular Surveillance of Antimalarial Resistance Pfcrt, Pfmdr1 and Pfk13 Polymorphisms in African Plasmodium Falciparum Imported Parasites to Wuhan, China

2020 ◽  
Author(s):  
Cheng Weijia ◽  
Xiaonan Song ◽  
Huabing Tan ◽  
Kai Wu ◽  
Jian Li
Keyword(s):  
Drug Resistance ◽  
Plasmodium Falciparum ◽  
Mixed Type ◽  
Antimalarial Drugs ◽  
Wild Type ◽  
Mutant Type ◽  
Molecular Surveillance ◽  
Severe Problem ◽  
Antimalarial Resistance ◽  
Effective Drugs

Abstract Background: The development of drug resistance in Plasmodium falciparum becomes a severe problem for malaria control globally. Before finding a practical solution, monitoring the susceptibility of P. falciparum resistance-related genes is crucial. It will offer valuable information on the drug resistance in malaria-endemic areas and guides the rational clinical use of antimalarial drugs.Methods：Filter paper blood was taken from patients with positive P. falciparum during 2017-2019 in Wuhan, China. The target fragments from pfcrt, pfmdr1, and k13 propeller (pfk13) genes of P. falciparum were amplified and sequenced. Subsequently, the polymorphisms of pfcrt, pfmdr1, and pfk13 and the haplotypes of Pfcrt and Pfmdr1 were analyzed.Results: Totally, 106 samples were collected. Subsequently, 98.11% (104/106), 100% (106/106), and 86.79% (92/106) of these samples were successfully amplified and sequenced for the pfcrt, pfmdr1, and pfk13 genes, respectively. The prevalence of Pfcrt K76T, Pfmdr1 N86Y, and Pfmdr1 Y184F mutation were 9.62%, 4.72%, and 47.17%, respectively. At codons 72-76 of pfcrt gene locus were showed three haplotypes, including CVMNK (wild-type), CVIET (mutation type), CV M/I N/E K/T (mixed type), with 87.50%, 9.62%, and 2.88% prevalence, respectively. For the pfmdr1 gene, including NY (wild type), NF and YF (mutant type), N Y/F, Y Y/F, and N/Y Y/F (mixed type), accounted for 34.91%, 43.40%, 3.77%, 15.09%, 0.94%, and 1.89%, respectively. A total of eleven Pfcrt/Pfmdr1 combined haplotypes, including six types of combined haplotypes, and five combined haplotypes with mixed-type, For pfk13, no mutation was detected. Conclusions: The wild-type SNPs and haplotypes for the pfcrt, and pfmdr1 genes become predominant in the current study. It indicates these isolates entirely or partly recovery their susceptibility to antimalarial drugs, including chloroquine, amodiaquine, and mefloquine. Moreover, it demonstrates these drugs can continue to be effective drugs for P. falciparum malaria cases treatment in Africa. Although no mutation is detected in pfk13, continuous molecular surveillance is still urgently necessary.

Malaria among migrants in a university hospital in Colombia during 2018: A case series

2020 ◽  
pp. 004947552098130
Author(s):  
Fabián R Carreño-Almánzar ◽  
Adán Coronado-Galán ◽  
Sonia A Cala-Gómez ◽  
Agustín Vega-Vera
Keyword(s):  
Latin American ◽  
Previous History ◽  
Case Series ◽  
Cross Sectional Study ◽  
Imported Malaria ◽  
University Hospital ◽  
Sectional Study ◽  
Cross Sectional ◽  
History Of ◽  
Clinical Profiles

Imported malaria has increased in Colombia since 2015 and has been attributed to migrants coming from Venezuela. We present a series of malaria cases, nested in a retrospective cross-sectional study between 2017 and 2018, aimed at calculating the prevalence of medical diseases among immigrants in a University Hospital in Colombia. Among 154 immigrants admitted for medical causes between 2017 and 2018, 8 were diagnosed with malaria, all due to Plasmodium vivax. Of these, seven had uncomplicated malaria, five had a previous history of malaria, one was critically ill, but none died. We highlight that, similar to other case series of imported malaria, Latin American migrants were young, with similar clinical profiles, having a low proportion of severe cases, and P. vivax was the most frequent cause.

scholarly journals Prevalence, characteristics and risk factors of imported and local malaria cases in North-Western Province, Zambia: a cross-sectional study

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Maureen N. Chipoya ◽  
Nzooma M. Shimaponda-Mataa
Keyword(s):  
Risk Factors ◽  
Cross Sectional Study ◽  
Malaria Prevalence ◽  
Imported Malaria ◽  
Full Blood Count ◽  
Sectional Study ◽  
Cross Sectional ◽  
Western Province ◽  
Imported Cases ◽  
North Western

Abstract Background Imported malaria is a major challenge for countries that are in malaria elimination stage such as Zambia. Legitimate cross-border activities add to the risk of transmission, necessitating determination of prevalence, characteristics and risk factors of imported and local malaria. Methods This cross-sectional study was conducted in 103 consented child and adult patients with clinical malaria symptoms, from selected health facilities in north-western Zambia. Patient demographic data and blood samples for malaria microscopy and full blood count were obtained. Chi-square and penalized logistic regression were performed to describe the characteristics and assess the risk factors of imported and local malaria in North-Western Province. Results Overall, malaria prevalence was 78.6% with 93.8% Plasmodium falciparum and 6.2% other species. The local cases were 72 (88.9%) while the imported were 9 (11.1%) out of the 81 positive participants. About 98.6% of the local cases were P. falciparum compared to 55.6% (χ2 = 52.4; p < 0.01) P. falciparum among the imported cases. Among the imported cases, 44% were species other than P. falciparum (χ2 = 48; p < 0.01) while among the local cases only 1.4% were. Gametocytes were present in 44% of the imported malaria cases and only in 2.8% of the local cases (χ2 = 48; p < 0.01). About 48.6% of local participants had severe anaemia compared to 33.3% of participants from the two neighbouring countries who had (χ2 = 4.9; p = 0.03). In the final model, only country of residence related positively to presence of species other than P. falciparum (OR = 39.0, CI [5.9, 445.9]; p < 0.01) and presence of gametocytes (OR = 23.1, CI [4.2, 161.6]; p < 0.01). Conclusion Malaria prevalence in North-Western Province is high, with P. falciparum as the predominant species although importation of Plasmodium ovale and Plasmodium malariae is happening as well. Country of residence of patients is a major risk factor for malaria species and gametocyte presence. The need for enhanced malaria control with specific focus on border controls to detect and treat, for specific diagnosis and treatment according to species obtaining, for further research in the role of species and gametocytaemia in imported malaria, cannot be overemphasized.

Detection of Genetic markers associated with Plasmodium falciparum drug resistance among malaria patients in Kaduna State, Nigeria

2021 ◽  
Vol 42 (2) ◽  
pp. 206-213
Author(s):  
G.Y. Benjamin ◽  
H.I. Inabo ◽  
M.H.I. Doko ◽  
B.O. Olayinka
Keyword(s):  
Drug Resistance ◽  
Plasmodium Falciparum ◽  
Phylogenetic Tree ◽  
Genetic Markers ◽  
Cross Sectional Study ◽  
Sub Saharan Africa ◽  
Health Concern ◽  
Cross Sectional ◽  
Blood Samples ◽  
Test Kit

Malaria is a disease of public health concern in Nigeria and sub-Saharan Africa. It is caused by intracellular parasites of the genus Plasmodium. The aim of this study was to detect genetic markers associated with Plasmodium falciparum drug resistance among malaria patients in Kaduna State, Nigeria. The study was a cross-sectional study that lasted from May 2018 to October 2018. Three hundred blood samples were collected from consenting individuals attending selected hospitals, in the three senatorial districts of Kaduna State, Nigeria. Structured questionnaire were used to obtain relevant data from study participants. The blood samples were screened for malaria parasites using microscopy and rapid diagnostic test kit. Polymerase Chain Reaction was used for detection of the drug resistance genes. Pfcrt, pfmdr1, pfdhfr, pfdhps and pfatpase6 genes were detected at expected amplicon sizes from the malaria positive samples. The pfatpase6 PCR amplicons were sequenced and a phylogenetic tree was created to determine their relatedness. Result showed that Pfcrt (80%) had the highest prevalence, followed by pfdhfr (60%), pfmdr1 (36%) and pfdhps (8%). Pfatpase6 was also detected in 73.3% of the samples, and a phylogenetic tree showed relatedness between the pfatpase6  sequences in this study and those deposited in the GenBank. In conclusion, the study detected that Plasmodium falciparum genes were associated with drug resistance to commonly used antimalarials.

scholarly journals Perceived threats towards COVID-19 pandemic among Nepali migrant workers returned from India

2021 ◽  
Vol 9 (01) ◽  
pp. 87-99
Author(s):  
Shanti Prasad Khanal ◽  
Edwin Van Teijlingen ◽  
Mohan Kumar Sharma ◽  
Jib Acharya ◽  
Sharma Sharma
Keyword(s):  
Migrant Workers ◽  
Health Workers ◽  
Health Belief ◽  
Personal Characteristics ◽  
Cross Sectional Study ◽  
Protective Behavior ◽  
Cross Sectional ◽  
Perceived Susceptibility ◽  
Belief Model ◽  
The Health Belief Model

Evidence shows that the seasonal migrants returned from India have been a source of COVID-19, which helped spread it across Nepal. This cross-sectional study, guided by the Health Belief Model (HBM) model, that people's underlying believes in this case around COVID-19, is determined by a large extend their subsequent behaviors. This study aimed to examine the association between personal characteristics and perceived threats regarding COVID-19 among 384 seasonal migrants who had returned from India and were staying in a quarantine center in Surkhet, Nepal. A questionnaire was used with the help of three health workers working in the Quarantine Center. A five-point sequential grade Likert scale was constructed based on two HBM constructs, like 'perceived susceptibility' and ‘severity.' A score ≥ of 50% was designated as high, and ≤ 50% as a perceived low level of threat. Univariate and bivariate analyses were performed to interpret the data using SPSS 20 version. Generally, participants regarded the threat of COVID-19 as low. The severity of COVID-19 was thought to be greater than the vulnerability of the subjects. Gender, age, caste, education, health status, and perceived susceptibility did not correlate with perceived susceptibility. However, the participants’ demographic characteristics were associated with their perceived level of severity. Therefore, this study calls for proper risk communication to people who returned from abroad, as this is essential in promoting protective behavior during a pandemic outbreak. 

scholarly journals A study on the pattern of skin diseases among migrant labourers visiting a teaching hospital

2018 ◽  
Vol 5 (8) ◽  
pp. 3600
Author(s):  
Pradeep Senapathi ◽  
Hemant Kumar ◽  
Shama Kamath ◽  
Nischitha S. ◽  
Mahesh V. ◽  
...  
Keyword(s):  
Teaching Hospital ◽  
Migrant Workers ◽  
Skin Diseases ◽  
Cross Sectional Study ◽  
Lower Limbs ◽  
Personal Hygiene ◽  
Cross Sectional ◽  
High Prevalence ◽  
Temporary Migrants ◽  
Infectious Skin Diseases

Background: Skin diseases are quite common among migrant workers, yet little research has been done to determine their prevalence and risk factors. Poor living conditions, over-crowding, low standards of personal hygiene and environmental sanitation make them even more vulnerable. The objective of the study was to assess the prevalence and pattern of skin infections among migrant labourers and their relationship with selected socio-demographic factors.Methods: A hospital based cross sectional study was carried out among 300 temporary migrants visiting dermatology OPD of A.J. Institute of Medical Sciences teaching hospital. The duration of study was two months. All patients were diagnosed clinically by qualified dermatologists and supported by relevant investigations.Results: Most of the migrants were males (62%) while remaining (38%) were females. Majority of the migrants (42.3%) belonged to 20-40 years age group. Infectious skin diseases were found among 64.3% of the study subjects while 34.7% of them had non-infectious skin diseases. The lesions were mainly over head and neck (23.3%) followed by abdomen (23%), upper limbs (21.3%) and lower limbs (21.3%).Conclusions: The study revealed a high prevalence of infectious skin diseases among the migrant workers.

scholarly journals Socio-economic Analysis and the Study of Prevalence, Awareness, Treatment, Control and Risk Factors of Hypertension in Hospital Staff

2013 ◽  
Vol 7 (1) ◽  
pp. 15-19
Author(s):  
Samjhana Shakya ◽  
J Bhattarai ◽  
K Rawal ◽  
AR Kunwar ◽  
YR Shakya ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Economic Status ◽  
Hospital Staff ◽  
Cross Sectional Study ◽  
Normal Weight ◽  
Overweight And Obesity ◽  
Cluster Sampling ◽  
Sectional Study ◽  
Cross Sectional ◽  
Public Health Challenge

Hypertension is an important public health challenge in the developing and the developed world alike. This worksite based descriptive cross-sectional study was undertaken in Sahid Gangalal National Heart Centre in 2010 with 50 subjects were recruited by multistage purposive cluster sampling. The primary aim of this descriptive cross-sectional study was to assess the socioeco­nomic status and to measure the prevalence, awareness, treatment, and correlates of hypertension. The prevalence of pre-hypertension is 38% and hypertension is 34%. Among the hypertensive 53% are aware of their condition and 66.66% of them are under treatment and 16.66% have con­trol over the condition. The prevalence of overweight and obesity were 22% and 4% respectively. The prevalence of central obesity was 29.72% among male and 69% among female. Knowledge, Attitude and Practice on hypertension was satisfactory. Fourteen percent understood hypertension as a raised blood pressure during blood flow in the arteries. Majority of them (72%) have posi­tive attitude towards use of medicine in hypertension. Majority of them (86%) had checked their blood pressure in last 2 years. Difference in prevalence of hypertension was found among the tobacco users and non- users (65% vs 40%), who do regular exercise and those who do not (28% vs. 38%) and sleep disturbance was higher among hypertensive than non-hypertensive (48% vs. 10%). Patients with Dislipidemia (32%) had higher prevalence of hypertension than those without Dislipidemia (44% vs 30 %) Similar finding were found between overweight participants (26%) and normal weight participants (46% vs 34%). The prevalence of hypertension is higher with the higher socio-economic status. Nepalese Heart Journal | Volume 7 | No.1 | November 2010 (special issue) | Page 15-19 DOI: http://dx.doi.org/10.3126/njh.v7i1.8496

scholarly journals PO 8563 UNCOMMONLY HIGH AND SYMPTOMATIC PLASMODIUM FALCIPARUM INFECTIONS IN GABONESE ADULTS

2019 ◽  
Vol 4 (Suppl 3) ◽  
pp. A53.3-A53
Author(s):  
Rella Zoleko Manego ◽  
Eric Koehne ◽  
Ghyslain Mombo-Ngoma
Keyword(s):  
Plasmodium Falciparum ◽  
Clinical Symptoms ◽  
Adult Population ◽  
Malaria Prevention ◽  
Cross Sectional Study ◽  
Sub Saharan Africa ◽  
High Rate ◽  
Temperature History ◽  
Public Health Issue ◽  
Cross Sectional

BackgroundMalaria remains a public health issue particularly in sub-Saharan Africa with special features of seriousness in young children and pregnant women. Older children and adults reported to have acquired a semi-immune status and therefore show low parasitaemia and less pronounced symptoms if any. We aim to explore parasitaemia and symptoms in adolescents and adults with malaria compared to younger children in the Lambaréné area.MethodsWe conducted a cross-sectional study in CERMEL for a month in 2018. Adults and children were screened for malaria at health facilities in Lambaréné and Fougamou and their respective surrounding villages in the central region of Gabon. Malaria infection was determined either by rapid diagnostic test (RDT) or by thick blood smear (TBS). Body temperature, history of fever, age, sex, haemoglobin level, and location were collected.ResultsFrom 09 January to 09 February 2018, a total of 596 patients were screened and 280 (47%) belonged to the adult population. Plasmodium falciparum infection in adults was diagnosed based on the detection of P. falciparum in thick blood films. Prevalence among the adults was 56% (n=141); 34% of adult patients had a parasite count between 1015–116,079 pf/µl. Fever was found in 31% of adults and several symptoms were reported by the same patient. Vomiting, loss of appetite, and fatigue were significantly more present in the adult population than in children.ConclusionThis study shows a high rate of Plasmodium falciparum infection in adults associated to clinical symptoms and high level of parasitaemia. Adults seem to become an at-risk population, suggesting that malaria programmes should consider including adults in the implementation of a malaria prevention programme.

scholarly journals Delay for First Consultation and Its Associated Factors among New Pulmonary Tuberculosis Patients of Central Nepal

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Wongsa Laohasiriwong ◽  
Roshan Kumar Mahato ◽  
Rajendra Koju ◽  
Kriangsak Vaeteewootacharn
Keyword(s):  
Pulmonary Tuberculosis ◽  
Associated Factors ◽  
Cross Sectional Study ◽  
Patient Delay ◽  
Cross Sectional ◽  
Tuberculosis Patients ◽  
Central Nepal ◽  
Delay Duration ◽  
Development Region ◽  
Public Health Challenge

Tuberculosis (TB) is still a major public health challenge in Nepal and worldwide. Most transmissions occur between the onset of symptoms and the consultation with formal health care centers. This study aimed to determine the duration of delay for the first consultation and its associated factors with unacceptable delay among the new sputum pulmonary tuberculosis cases in the central development region of Nepal. An analytical cross-sectional study was conducted in the central development region of Nepal between January and May 2015. New pulmonary sputum positive tuberculosis patients were interviewed by using a structured questionnaire and their medical records were reviewed. Among a total of 374 patients, the magnitude of patient delay was 53.21% (95% CI: 48.12–58.28%) with a median delay of 32 days and an interquartile range of 11–70 days. The factors associated with unacceptable patient delay (duration ≥ 30 days) were residence in the rural area (adj. OR = 3.10, 95% CI: 1.10–8.72;pvalue = 0.032) and DOTS center located more than 5 km away from their residences (adj. OR = 5.53, 95% CI: 2.18–13.99;pvalue < 0.001). Unemployed patients were more likely to have patient delay (adj. OR = 7.79, 95% CI: 1.64–37.00;pvalue = 0.010) when controlled for other variables.

Sign in / Sign up

Export Citation Format

Share Document