scholarly journals Serum biochemistry and haematology in wild and captive bearded seals (Erignathus barbatus) from Svalbard, Norway

2021 ◽  
Vol 63 (1) ◽  
Author(s):  
Morten Tryland ◽  
Christian Lydersen ◽  
Kit Maureen Kovacs ◽  
Espen Rafter ◽  
Stein Istre Thoresen
Keyword(s):  
Reference Values ◽  
Physical Restraint ◽  
Serum Biochemistry ◽  
Life Cycles ◽  
Physiological Parameters ◽  
Clinical Samples ◽  
Specific Reference ◽  
Serum Chemistry ◽  
Blood Samples ◽  
Bearded Seals

Abstract Background Health assessment of seals in captivity include haematology and serum biochemistry measurements. Because such parameters differ between species, it is crucial to have species-specific reference values for the interpretation of clinical samples. Furthermore, differences in nutrition and environment, life cycles as well as seasonal/annual cycles and varying physiological conditions can potentially affect serum chemistry and haematology parameters. Blood samples from four captive adult bearded seals (initially caught as pups in Svalbard, Norway, now held at Polaria, an Arctic experience centre in Tromsø, Norway) collected over a 16-month period were analysed for haematology (n = 22) and serum chemistry (n = 25) parameters. Serum chemistry analyses were also conducted on blood samples from 74 wild bearded seals (1995–2007) collected from Svalbard, Norway. Results We found higher activity of creatine kinase (CK) and higher concentrations of cortisol in the wild animals when compared to the captive seals, probably reflecting the physical restraint and concomitant stress induced during sampling. For the captive bearded seals, we did not find marked differences in haematology or serum chemistry parameters throughout the different seasons of sampling. Conclusions This study presents haematology and serum chemistry reference values for captive and wild bearded seals. Comparing physiological parameters for captive seals with wild seals indicated that having wild-caught bearded seals under the conditions offered at Polaria for several years did not markedly affect physiological parameters of the animals, and that training may have helped to alleviate stress associated with blood sampling and veterinary inspection.

scholarly journals Reference values for chinchilla (Chinchilla laniger) blood cells and serum biochemical parameters

2005 ◽  
Vol 35 (3) ◽  
pp. 602-606 ◽  
Author(s):  
Tális de Oliveira Silva ◽  
Luiz Carlos Kreutz ◽  
Leonardo José Gil Barcellos ◽  
João Borella ◽  
Auren Benck Soso ◽  
...  
Keyword(s):  
Health Status ◽  
Reference Values ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
Serum Biochemistry ◽  
Serum Biochemical Parameters ◽  
Adult Males ◽  
Blood Samples ◽  
Serum Biochemical ◽  
South Brazil

Raising chinchilla (Chinchilla laniger) for commercial purpose has increased significantly; however, hematological and serum biochemical reference values have not yet been determined for chinchillas raised in south Brazil. Establishing blood cells and serum biochemistry reference values might be helpful to evaluate health status of chinchillas and might be used as a tool by clinicians. The purpose of this study was to determine the reference values for blood cells and serum biochemistry of Chinchilla laniger. Blood samples were collected by cardiac puncture from 16 adult males, at the time they were killed to remove the fur coat, and from 8 adult males anesthetized with ketamine and xylazine. Blood cell counts and serum biochemistry analysis were performed using standard techniques and the results were expressed as mean ± SEM. Analysis of blood parameters from post-mortem cardiac punctured and from anesthetized chinchillas indicated that blood samples from anesthetized chinchillas had higher PCV, Hemoglobin, MCHC and WBC (P < .05); in contrast, had lower levels of monocytes, basophils and eosinophils (P < .05). Serum biochemical parameters were less affected by sampling method: anesthetized chinchillas had lower levels of urea, glucose and triglycerids (P < .05). The data obtained might be useful as a parameter to monitor the health status of chinchillas raised in south Brazil.

scholarly journals Complex Reference Values for Endocrine and Special Chemistry Biomarkers across Pediatric, Adult, and Geriatric Ages: Establishment of Robust Pediatric and Adult Reference Intervals on the Basis of the Canadian Health Measures Survey

2015 ◽  
Vol 61 (8) ◽  
pp. 1063-1074 ◽  
Author(s):  
Khosrow Adeli ◽  
Victoria Higgins ◽  
Michelle Nieuwesteeg ◽  
Joshua E Raizman ◽  
Yunqi Chen ◽  
...  
Keyword(s):  
Reference Values ◽  
Reference Value ◽  
Reference Intervals ◽  
Specific Reference ◽  
Blood Samples ◽  
Health Measures ◽  
Laboratory Test Results ◽  
Canadian Health ◽  
Canadian Health Measures Survey ◽  
Age And Sex

Abstract BACKGROUND Defining laboratory biomarker reference values in a healthy population and understanding the fluctuations in biomarker concentrations throughout life and between sexes are critical to clinical interpretation of laboratory test results in different disease states. The Canadian Health Measures Survey (CHMS) has collected blood samples and health information from the Canadian household population. In collaboration with the Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER), the data have been analyzed to determine reference value distributions and reference intervals for several endocrine and special chemistry biomarkers in pediatric, adult, and geriatric age groups. METHODS CHMS collected data and blood samples from thousands of community participants aged 3 to 79 years. We used serum samples to measure 13 immunoassay-based special chemistry and endocrine markers. We assessed reference value distributions and, after excluding outliers, calculated age- and sex-specific reference intervals, along with corresponding 90% CIs, according to CLSI C28-A3 guidelines. RESULTS We observed fluctuations in biomarker reference values across the pediatric, adult, and geriatric age range, with stratification required on the basis of age for all analytes. Additional sex partitions were required for apolipoprotein AI, homocysteine, ferritin, and high sensitivity C-reactive protein. CONCLUSIONS The unique collaboration between CALIPER and CHMS has enabled, for the first time, a detailed examination of the changes in various immunochemical markers that occur in healthy individuals of different ages. The robust age- and sex-specific reference intervals established in this study provide insight into the complex biological changes that take place throughout development and aging and will contribute to improved clinical test interpretation.

Isolation of Stenotrophomonas maltophilia from Clinical Samples in Some Hospitals in Shiraz, Iran

2020 ◽  
pp. 1-3
Author(s):  
Majid Baserisalehi ◽  
Samira Zarezadeh ◽  
Majid Baserisalehi ◽  
Saeed Shoa
Keyword(s):  
Stenotrophomonas Maltophilia ◽  
Enterobacter Aerogenes ◽  
Late Summer ◽  
Early Spring ◽  
Clinical Samples ◽  
Bacillus Species ◽  
Microbiological Analysis ◽  
Blood Samples ◽  
Healthcare Facilities ◽  
Phenotypic Methods

Stenotrophomonas maltophilia is an emerging pathogenic non-fermentative Gram-negative Bacillus species. It has caused many nosocomial infections and can be isolated from various hospital wards and healthcare facilities. Research has shown that most of its strains are inherently resistant to many antibiotics and have multidrug resistance. This research intended to determine its occurrence frequency at some Hospitals in shiraz, Iran. The present study was conducted in six months (from early spring to late summer 2019). Clinical samples (Blood, Urine and cerebrospinal fluid (CSF)) collected from 120 patients afflicted with various infections. The samples were transferred to the Laboratory and subjected to microbiological analysis. Identification of the isolates was carried out by phenotypic methods and Stenotrophomonas maltophilia isolates verified using molecular methods. In total, various bacteria were isolated from 84 clinical samples. The isolates were Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Stenotrophomonas maltophilia, Staphylococcus aureus and Pseudomonas aeruginosa. Stenotrophomonas maltophilia was isolated from 17 (20.2%) positive samples and most of them were isolated from blood samples. Our finding indicated that Stenotrophomonas maltophilia isolated more from blood samples follow by CSF sample. In addition, our finding illustrated that Stenotrophomonas maltophilia can be considered as the common nosocomial agent at hospitals in Shiraz, Iran.

P117. Bone density distribution in the cervical spine: Establishing level specific reference values

2021 ◽  
Vol 21 (9) ◽  
pp. S197-S198
Author(s):  
Francis C. Lovecchio ◽  
Bryan Ang ◽  
Philip Louie ◽  
Chirag Chaudhary ◽  
Sachin Shah ◽  
...  
Keyword(s):  
Cervical Spine ◽  
Bone Density ◽  
Density Distribution ◽  
Reference Values ◽  
Specific Reference

Osmolal and Anion Gaps in Patients Admitted to an Emergency Medical Department

1994 ◽  
Vol 13 (2) ◽  
pp. 131-134 ◽  
Author(s):  
Lars Aabakken ◽  
Kjerstin S. Johansen ◽  
Else-Berit Rydningen ◽  
Jan E. Bredesen ◽  
Steinar Øvrebø ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Ethylene Glycol ◽  
Reference Values ◽  
Medical Department ◽  
Blood Samples ◽  
Emergency Medical ◽  
Unselected Population ◽  
The Mean ◽  
Lactate Levels ◽  
Osmolal Gap

1 Osmolal and anion gaps are helpful in the diagnosis and evaluation of intoxications with methanol and ethylene glycol. Reported reference values for osmolal gap and anion gap are -1 (± 6) mosm kg-1 H2O and 16 (± 2) mmol I -1, respectively. However, we have repeatedly found unexplained increased gaps in patients admitted to our department, and the relevance of the established reference values has been questioned. 2 Osmolal and anion gaps were determined in an unselected population of patients consecutively admitted to an emergency medical department. In the case of unexplained gaps, the blood samples were analysed with respect to the presence of alcohols and organic acids. 3 We included all accessible patients admitted during 14 days. Appropriate blood samples were obtained in 177 patients (88 male, 89 female), with a mean age of 65 years (range 17-94). 4 The mean and (standard deviation) for osmolal and anion gaps in our material were 5.2 mosm kg-1 H2O (7.0) and 12.9 mmol/l (4.2). Neither methanol nor ethylene-glycol was detected in serum from any patients. Small amounts of ethanol were found in 5 patients, and high lactate levels explained in part the most extensively increased anion gaps. However, the calculated analytical standard deviation accounted entirely for the variation in our material, and we suggest that the present reference values be adjusted.

Comparative study of i-SENS glucometers in neonates using capillary blood samples

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ha Nui Kim ◽  
Soo-Young Yoon
Keyword(s):  
Reference Values ◽  
Point Of Care ◽  
Evaluation Process ◽  
Capillary Blood ◽  
Performance Criteria ◽  
Accuracy Evaluation ◽  
Blood Samples ◽  
Average Reference ◽  
Error Grid ◽  
Accuracy Performance

Abstract Objectives The accuracy of point-of-care blood glucometers in the detection and evaluation of neonatal hypoglycemia is a concern. This study compared the performance of three i-SENS glucometers with that of the YSI 2300 STAT Plus Analyzer, which was used as a reference. Methods The leftover neonatal capillary blood samples of 319 patients were used in this study. The evaluation process and accuracy performance criteria were based on the International Organization for Standardization 15197:2013 guidelines. The evaluation involved three i-SENS glucometers (BAROzen H Expert plus, CareSens PRO, and CareSens H Beat) and the ACCU-CHEK® Inform II glucometer. Results The accuracy evaluation yielded acceptable results as follows: a) 100 and 100% for BAROzen H Expert plus; 99.8 and 100% for CareSens PRO; 98.7%, and 97.2% for CareSens H Beat glucometers were within the range of ±0.8 mmol/L (15 mg/dL) and ±15% of the average reference values at glucose concentrations <5.55 mmol/L (100 mg/dL) and ≥5.55 mmol/L (100 mg/dL), respectively, and b) all estimated glucose values (100%) were within the zones A and B of Consensus Error Grid for all three i-SENS glucometers. There was good correlation between the glucose values estimated by the glucometers and the reference values (R>0.990). Conclusions This study demonstrated that i-SENS glucometers exhibit acceptable performance and can be used as effective point-of-care devices in neonates.

Intra- and inter-day variability in plasma tCO2 concentration in sedentary horses

2014 ◽  
Vol 10 (1) ◽  
pp. 39-47
Author(s):  
B.E. Bergstrom ◽  
J.H. Foreman ◽  
C.R. Foreman ◽  
A.M. Barger
Keyword(s):  
Blood Test ◽  
Indoor Environment ◽  
Early Morning ◽  
Time Of Day ◽  
Individual Variability ◽  
Strong Ion Difference ◽  
Serum Chemistry ◽  
Blood Samples ◽  
Early Afternoon ◽  
Glass Tubes

Sodium bicarbonate and other alkalinising solutions (‘milkshakes’) have been given to horses surreptitiously before exercise to provide exogenous buffering effects. After an initial positive blood test, some accused horse trainers claim that their horses ‘naturally test high’, so some jurisdictions allow a secured quarantine in which the horse is tested multiple times. The objective of this experiment was to determine the intra- and inter-day variability of plasma total CO2 (tCO2) and other plasma strong ions in a group of sedentary horses housed similarly to a quarantine period. The hypothesis was that plasma tCO2 would not remain constant over a multi-day monitoring interval, but would vary measurably during that interval. Eight sedentary (unconditioned) horses were studied for 2 weeks. Horses were acclimated to a climate-controlled indoor environment and an alfalfa-only diet for a minimum of 10 days prior to sampling. Horses were sampled 3 times daily for 5 consecutive days at 7:00, 11:00 and 15:00 h. Blood samples were collected directly into 10 ml heparinised evacuated glass tubes by jugular venipuncture using a double-ended 0.91 mm needle. Samples were chilled until concentrations of plasma tCO2, Na+, K+, and Cl-, were determined within 1-3 h of sampling using an automated serum chemistry analyzer which was calibrated daily using commercial reagents obtained from the manufacturer as well as externally-obtained NIST-traceable calibrating solutions. Mean results documented mild variations in mean plasma tCO2 (range 28.9-31.6 mmol/l), but individual horses’ plasma tCO2 ranged over 4-7 units. Results showed that there was considerable intra- and inter-individual variability in plasma tCO2. Mean pooled tCO2 and measured strong ion difference (SIDm) differed by time-of-day, with both late morning and early afternoon values lower than early morning values (P<0.001). There was a strong positive linear relationship between plasma SIDm and tCO2 (r=0.75, P<0.001).

scholarly journals Effect of the Clenbuterol drug in some physiological parameters in male rabbits(Oryctolagus cuniculs)

2016 ◽  
Vol 13 (4) ◽  
pp. 639-648
Author(s):  
Baghdad Science Journal
Keyword(s):  
Kidney Function ◽  
Adult Male ◽  
Biochemical Study ◽  
Study Drug ◽  
Physiological Parameters ◽  
The Other ◽  
Distilled Water ◽  
Water Control ◽  
Blood Samples

This study was designed to investigate the effect of the Clenbuterol drug in some physiological parameters of Liver, Kidney function and lipidprofie concentration. Fourty adult male rabbits were used in this study. The animals were randomly divided into four groups,the first group was given distilled water (control) ,while the other third groups were given (2,4,6 µg/kg) of Clenbuterol drug for 30 days. Blood samples were collected after 14 and 30 days of the treatment for biochemical study. Drug caused non significant increase(P

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