scholarly journals The relative expression of miR-31, miR-29, miR-126, and miR-17 and their mRNA targets in the serum of COVID-19 patients with different grades during hospitalization

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Reza Keikha ◽  
Seyed Mohammad Hashemi-Shahri ◽  
Ali Jebali
Keyword(s):  
Cross Sectional Study ◽  
Health Centers ◽  
Sectional Study ◽  
Medical Sciences ◽  
Serum Samples ◽  
Cross Sectional ◽  
Relative Expression ◽  
Diagnosis And Prognosis ◽  
Mrna Targets ◽  
Disease Grade

Abstract Background The aim of this study was to evaluate the expression of four up/down-regulated inflammatory miRNAs and their mRNA targets in the serum samples of COVID-19 patients with different grades. Also, we investigated the relative expression of these miRNAs and mRNAs during hospitalization. Methods In this cross-sectional study, 5 mL of blood sample were taken from COVID-19 patients with different grades and during hospitalization from several health centers of Yazd, Tehran, and Zahedan province of Iran from December 20, 2020 to March 2, 2021. The relative expression of miRNAs and mRNAs was evaluated by q-PCR. Results We found that the relative expression of hsa-miR-31-3p, hsa-miR-29a-3p, and hsa-miR-126-3p was significantly decreased and the relative expression of their mRNA targets (ZMYM5, COL5A3, and CAMSAP1) was significantly increased with the increase of disease grade. Conversely, the relative expression of hsa-miR-17-3p was significantly increased and its mRNA target (DICER1) was significantly decreased with the increase of disease grade. This pattern was exactly seen during hospitalization of COVID-19 patients who did not respond to treatment. In COVID-19 patients who responded to treatment, the expression of selected miRNAs and their mRNA targets returned to the normal level. A negative significant correlation was seen between (1) the expression of hsa-miR-31-3p and ZMYM5, (2) hsa-miR-29a-3p and COL5A3, (3) hsa-miR-126-3p and CAMSAP1, and (4) hsa-miR-17-3p and DICER1 in COVID-19 patients with any grade (P  <  0.05) and during hospitalization. Conclusions In this study, we gained a more accurate understanding of the expression of up/down-regulated inflammatory miRNAs in the blood of COVID-19 patients. The obtained data may help us in the diagnosis and prognosis of COVID-19. Trial registration: The ethics committee of Zahedan University of Medical Sciences, Zahedan, Iran. (Ethical Code: IR.ZAUMS.REC.1399.316) was registered for this project.

Seroprevalence of dengue in blood donors in an outbreak: experience of a blood bank in north India

2019 ◽  
Vol 49 (3) ◽  
pp. 212-215 ◽  
Author(s):  
Ashish Jain ◽  
Sanchit Jain ◽  
Nilotpal Chowdhury
Keyword(s):  
Blood Donors ◽  
Rapid Test ◽  
Cross Sectional Study ◽  
Blood Bank ◽  
North India ◽  
Prevalence Rates ◽  
Sectional Study ◽  
Medical Sciences ◽  
Serum Samples ◽  
Cross Sectional

Our aim was to determine the prevalence of dengue among blood donors at a centre in north India during an outbreak. A retrospective cross-sectional study was conducted using stored serum samples of 369 blood donors at the All India Institute of Medical Sciences (AIIMS), Rishikesh between August and November 2016. Serum samples were tested by an immune-chromatographic rapid test for the screening of dengue markers (IgG/IgM and NS-1). We found prevalence rates of IgG and NS-1 to be 14.9 and 0.54%, respectively.

scholarly journals E-booking Websites in Iranian Public Clinics: A Step Toward Health Equity

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Ali Garavand ◽  
Nasim Aslani ◽  
Seyyed Mohammad Ayyoubzadeh ◽  
Shahabeddin Abhari
Keyword(s):  
Waiting Time ◽  
Healthcare Services ◽  
Cross Sectional Study ◽  
Health Centers ◽  
Sectional Study ◽  
Medical Sciences ◽  
Situation Analysis ◽  
Cross Sectional ◽  
Patients Satisfaction ◽  
Public Clinics

Background: Scheduling doctor appointments at healthcare centers have been a managerial challenge for health systems. In recent years, many efforts have been made to implement e-booking websites in medical universities and their affiliated healthcare centers. Objectives: The aim of this study was to analyze the recent situation of e-booking in the medical sciences affiliated clinics. Methods: The present study was a descriptive cross-sectional study conducted in 2019 in 31 provinces, universities of medical sciences, and affiliated health centers. A checklist with 11 items was used for situation analysis. Results: The results showed that just 5 out of 31 (15.15%) provinces and their affiliated universities of medical sciences lacked an e-booking system. Moreover, 85.71% of cases had an integrated e-booking portal, 67.86% had mobile-based applications for e-booking, and just 14.29% of the cases had online payment gateway that was the lowest score in all items studied. Conclusions: Based on the results of this study, there is a significant growth in the situation of e-booking websites in Iran in the last two years. If this improvement continues, it can lead to equity development in healthcare services, reduction of patients’ waiting time, a decrease in waste of resources, and an increase in patients’ satisfaction.

Disposal Practices of Unused Medications Among Patients in Public Health Centers of Dessie Town, Northeast Ethiopia: A Cross-sectional Study

2020 ◽  
Vol 15 (2) ◽  
pp. 105-110
Author(s):  
Haile Kassahun ◽  
Dugessa Tesfaye
Keyword(s):  
Public Health ◽  
Health Care Professionals ◽  
Cross Sectional Study ◽  
Health Centers ◽  
Sectional Study ◽  
Cross Sectional ◽  
Global Challenge ◽  
Northeast Ethiopia ◽  
Unused Medications ◽  
At Home

Background: Disposal of pharmaceutical waste among patients is a global challenge especially in developing countries like Ethiopia. Improper medication disposal can lead to health problems and environmental contaminations. Therefore, the present study aimed to assess disposal practices of unused medications among patients in public health centers of Dessie town, Northeast Ethiopia. Methods: A descriptive cross-sectional study was conducted among 263 patients in four public health centers of Dessie town, Ethiopia from March to June, 2019. Face-to-face interviews using structured questionnaires were used to collect data from each study subject. Results: The majority of the respondents, 224 (85.17%) had unused medications at their home during the study period. The most commonly reported disposal method in the present study was flushing down into a toilet 66 (25.09%). None of the respondents practiced returning unused medications to Pharmacy. Moreover, 85 (32.31%) of the respondents reported never disposing their medications and believed that it is acceptable to store medications at home for future use. Conclusion: In the present study, there was a high practice of keeping medications at home and most of the disposal practices were not recommended methods. In addition, most of the respondents did not get advice from pharmacists and other health care professionals on how to dispose off unused medications. Hence, there is a need for proper education and guidance of patients regarding disposal practices of unused medications.

scholarly journals Serum Concentrations of Selected Organochlorines in Pregnant Women and Associations with Pregnancy Outcomes. A Cross-Sectional Study from Two Rural Settings in Cambodia

2020 ◽  
Vol 17 (20) ◽  
pp. 7652
Author(s):  
Margit Steinholt ◽  
Shanshan Xu ◽  
Sam Ol Ha ◽  
Duong Trong Phi ◽  
Maria Lisa Odland ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Pregnant Women ◽  
Gestational Age ◽  
Low Income ◽  
Cross Sectional Study ◽  
Maternal Serum ◽  
Sectional Study ◽  
Serum Samples ◽  
Cross Sectional ◽  
Rural Settings

We conducted a cross-sectional study among 194 pregnant women from two low-income settings in Cambodia. The inclusion period lasted from October 2015 through December 2017. Maternal serum samples were analyzed for persistent organic pollutants (POPs). The aim was to study potential effects on birth outcomes. We found low levels of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCP), except for heptachlors, β-hexachlorocyclohexane (HCH), heptachlor epoxide, and p,p’-DDE. There were few differences between the two study locations. However, the women from the poorest areas had significantly higher concentrations of p,p’-DDE (p < 0.001) and hexachlorobenzene (HCB) (p = 0.002). The maternal factors associated with exposure were parity, age, residential area, and educational level. Despite low maternal levels of polychlorinated biphenyls, we found significant negative associations between the PCB congeners 99 (95% CI: −2.51 to −0.07), 138 (95% CI: −1.28 to −0.32), and 153 (95% CI: −1.06 to −0.05) and gestational age. Further, there were significant negative associations between gestational age, birth length, and maternal levels of o,p’-DDE. Moreover, o,p’-DDD had positive associations with birth weight, and both p,p’-DDD and o,p’-DDE were positively associated with the baby’s ponderal index. The poorest population had higher exposure and less favorable outcomes.

scholarly journals Agreement between patients’ and radiation oncologists’ cancer diagnosis and prognosis perceptions: A cross sectional study in Japan

PLoS ONE ◽  
2018 ◽  
Vol 13 (6) ◽  
pp. e0198437 ◽  
Author(s):  
Lisa Jane Mackenzie ◽  
Mariko Leanne Carey ◽  
Eiji Suzuki ◽  
Robert William Sanson-Fisher ◽  
Hiromi Asada ◽  
...  
Keyword(s):  
Cancer Diagnosis ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Cross Sectional ◽  
Diagnosis And Prognosis ◽  
Radiation Oncologists ◽  
Cancer Diagnosis And Prognosis

scholarly journals Hypertension and its Relation with Waist to Hip Ratio in Women Referred to Bojnurd Urban Health Centers in 2014

2019 ◽  
Vol 11 (1) ◽  
pp. 1-5
Author(s):  
Hossein Lashkardoost ◽  
Fateme Hoseyni ◽  
Elham Rabbani ◽  
Farzane Q Moqadam ◽  
Leila Hosseini ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Pearson Correlation ◽  
Cross Sectional Study ◽  
Health Centers ◽  
Sectional Study ◽  
Chi Square ◽  
Cross Sectional ◽  
Waist To Hip Ratio ◽  
Significant Relationships ◽  
Present Cross Sectional Study

Introduction: North Khorasan province has one of the highest rates of hypertension. One of the main causes of hypertension is obesity. Obesity is one of the most important public health problems around the world as a risk factor for non-communicable diseases. Since a similar study was not conducted in Bojnurd, we examined the relationship between waist to hip ratio with hypertension. Methods: The present cross-sectional study was conducted on women referring to Bojnurd health centers. To analyze the data, we used t-test, chi-square, multiple logistic regression and Pearson correlation in Stata 12 software. Results: In this cross-sectional study, the prevalence of systolic blood pressure was 14.78% and diastolic blood pressure was 15.65%. So waist to the hips showed the highest correlation with the changes in hypertension. Conclusion: There are significant relationships between the age and the number of pregnancies with the risk of hypertension. Since a significant percentage of people are unaware of the existence of hypertension, changing diet and having regular physical activity along with social support is an important strategy.

scholarly journals Perception towards Online Classes during COVID-19 among MBBS and BDS Students in a Medical College of Nepal: A Descriptive Cross-sectional Study

2021 ◽  
Vol 59 (235) ◽  
Author(s):  
Nitasha Sharma ◽  
Chet Kant Bhusal ◽  
Sandip Subedi ◽  
Rajeshwar Reddy Kasarla
Keyword(s):  
Teaching Hospital ◽  
Cross Sectional Study ◽  
Online Classes ◽  
Sectional Study ◽  
Medical Sciences ◽  
Cross Sectional ◽  
Dental Surgery ◽  
Face To Face ◽  
Medical College ◽  
Medical Colleges

Introduction: Sudden outbreak of COVID-19 pandemic has affected the educational system worldwide, forced the medical colleges to close due to lock down, and disrupted the classroom face-to-face teaching process. As a result, medical colleges shifted to an online mode of teaching. The aim of this study is to find out the perception towards online classes during COVID-19 lockdown period among MBBS and BDS students at a medical college of Nepal. Methods: This was a descriptive cross-sectional study carried out at Universal College of Medical Sciences and Teaching Hospital among first and second year Bachelor in Medicine, Bachelor of Surgery and Bachelor in dental surgery students from 1st June 2020 to 30th August 2020. Ethical approval was taken from Institutional Review Committee of Universal College of Medical Sciences and Teaching Hospital (IRC UCMS, Ref: UCMS/IRC/025/20). Convenient sampling method was used. Semi-structured questionnaire was used. Statistical Package for Social Sciences 22 was used for analysis and frequency and percentage was calculated. Results: One hundred fifty six (73.93%) students were enjoying online learning only to some extent, 135 (63.98%) felt online class not equally effective as face-to-face teaching. The students had disturbance during online classes as internet disturbance 168 (79.60%), and electricity problem 47 (22.3%). Similarly, many students 155 (73.50%) felt external disturbance, headache 26 (12.3%), and eye strain 26 (12.3%). Conclusions: Most of the students suffered from disturbances during online classes probably because of internet and electricity problem. When compulsory to conduct online classes, students felt that not more than three online classes per day should be conducted to avoid eye strain and headache.

scholarly journals A cross sectional study of leptospirosis and fetal death in Yucatan, Mexico

2016 ◽  
pp. 11-14 ◽  
Author(s):  
María Fidelia Cárdenas Marrufo ◽  
Ignacio Vado Solis ◽  
Gaspar Fernando Peniche Lara ◽  
Carlos Perez Osorio ◽  
José Correa Segura
Keyword(s):  
Spontaneous Abortion ◽  
Low Income ◽  
Fetal Death ◽  
Indirect Elisa ◽  
First Trimester ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Serum Samples ◽  
Recent Infection ◽  
Cross Sectional

Introduction: Leptospirosis is a zoonotic disease affecting mainly to low income human population. Acute leptospiral infection during pregnancy has been associated with spontaneous abortion and fetal death during the first trimester and the abortion may occur as consequence of systemic failure. Objective: To estimate the frequency of Leptospira interrogans infection in women with spontaneous abortion in the state of Yucatan, Mexico. Methods: A cross sectional study on women with spontaneous abortion was conducted. Serum samples were tested for Leptospirosis by the microaglutination test, to estimate the frequency of the infecting serovar. The indirect ELISA IgM was used to detect recent infection by L. interrogans. DNA was extracted from paraffin-embedded tissue of placenta for PCR detection of L. interrogans. Results: Overall frequency of infection with L. interrogans in the 81 women with abortion was 13.6%. Five of the 12 serovars evaluated were found and included. Two of the 11 women with abortion and positive to microaglutination test were also positive to the ELISA IgM test. None samples were positive for PCR Leptospira diagnosis. Conclusion: two women could be associated with spontaneous abortion due to leptospirosis, because they showed antibodies against L. interrogans in the microaglutination test and ELISA IgM assays. Differences between regions were found with respect to the prevalences of lesptospirosis.

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