scholarly journals Laccases as green and versatile biocatalysts: from lab to enzyme market—an overview

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Tatiane Brugnari ◽  
Dayane Moreira Braga ◽  
Camila Souza Almeida dos Santos ◽  
Bruno Henrique Czelusniak Torres ◽  
Tatiani Andressa Modkovski ◽  
...  

AbstractLaccases are multi-copper oxidase enzymes that catalyze the oxidation of different compounds (phenolics and non-phenolics). The scientific literature on laccases is quite extensive, including many basic and applied research about the structure, functions, mechanism of action and a variety of biotechnological applications of these versatile enzymes. Laccases can be used in various industries/sectors, from the environmental field to the cosmetics industry, including food processing and the textile industry (dyes biodegradation and synthesis). Known as eco-friendly or green enzymes, the application of laccases in biocatalytic processes represents a promising sustainable alternative to conventional methods. Due to the advantages granted by enzyme immobilization, publications on immobilized laccases increased substantially in recent years. Many patents related to the use of laccases are available, however, the real industrial or environmental use of laccases is still challenged by cost–benefit, especially concerning the feasibility of producing this enzyme on a large scale. Although this is a compelling point and the enzyme market is heated, articles on the production and application of laccases usually neglect the economic assessment of the processes. In this review, we present a description of laccases structure and mechanisms of action including the different sources (fungi, bacteria, and plants) for laccases production and tools for laccases evolution and prediction of potential substrates. In addition, we both compare approaches for scaling-up processes with an emphasis on cost reduction and productivity and critically review several immobilization methods for laccases. Following the critical view on production and immobilization, we provide a set of applications for free and immobilized laccases based on articles published within the last five years and patents which may guide future strategies for laccase use and commercialization.

2020 ◽  
Vol 19 (1) ◽  
pp. 13
Author(s):  
Leury Max Da Silva Chaves ◽  
Gabriel Vinicius Santos ◽  
Cauê La Scala Teixeira ◽  
Marzo Edir Da Silva-Grigoletto

 Bodyweight exercises (also popularly known as calisthenics) is a classic training method and its practice has been widespread since the 19th century, but little evidenced in the scientific literature over the years. This type of training aims to promote multi-system adaptations using body weight as an overload with no or few implements [1–3]. This characteristic makes exercise with body weight easy to apply, in addition to having an excellent cost-benefit ratio when compared to other training possibilities that require machines or materials [4,5].


2020 ◽  
pp. 165-171
Author(s):  
Iryna Hryhoruk

Exhaustion of traditional energy resources, their uneven geographical location, and catastrophic changes in the environment necessitate the transition to renewable energy resources. Moreover, Ukraine's economy is critically dependent on energy exports, and in some cases, the dependence is not only economic but also political, which in itself poses a threat to national security. One of the ways to solve this problem is the large-scale introduction and use of renewable energy resources, bioenergy in particular. The article summarizes and offers methods for assessing the energy potential of agriculture. In our country, a significant amount of biomass is produced every year, which remains unused. A significant part is disposed of due to incineration, which significantly harms the environment and does not allow earning additional funds. It is investigated that the bioenergy potential of agriculture depends on the geographical distribution and varies in each region of Ukraine. Studies have shown that as of 2019 the smallest share in the total amount of conventional fuel that can be obtained from agricultural waste and products suitable for energy production accounts for Zakarpattya region - 172.5 thousand tons. (0.5% of the total) and Chernivtsi region - 291.3 thousand tons. (0.9%). Poltava region has the greatest potential - 2652.2 thousand tons. (7.8%) and Vinnytsia - 2623.7 thousand tons. (7.7%). It should be noted that the use of the energy potential of biomass in Ukraine can be called unsatisfactory. The share of biomass in the provision of primary energy consumption is very small. For bioenergy to occupy its niche in the general structure of the agro-industrial complex, it is necessary to develop mechanisms for its stimulation. In addition, an effective strategy for the development of the bioenergy sector of agriculture is needed. The article considers the general energy potential of agriculture, its indicative structure. The analysis is also made in terms of areas. In addition, an economic assessment of the possible use of existing potential is identified.


Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 661
Author(s):  
Luigi Piazzi ◽  
Stefano Acunto ◽  
Francesca Frau ◽  
Fabrizio Atzori ◽  
Maria Francesca Cinti ◽  
...  

Seagrass planting techniques have shown to be an effective tool for restoring degraded meadows and ecosystem function. In the Mediterranean Sea, most restoration efforts have been addressed to the endemic seagrass Posidonia oceanica, but cost-benefit analyses have shown unpromising results. This study aimed at evaluating the effectiveness of environmental engineering techniques generally employed in terrestrial systems to restore the P. oceanica meadows: two different restoration efforts were considered, either exploring non-degradable mats or, for the first time, degradable mats. Both of them provided encouraging results, as the loss of transplanting plots was null or very low and the survival of cuttings stabilized to about 50%. Data collected are to be considered positive as the survived cuttings are enough to allow the future spread of the patches. The utilized techniques provided a cost-effective restoration tool likely affordable for large-scale projects, as the methods allowed to set up a wide bottom surface to restore in a relatively short time without any particular expensive device. Moreover, the mats, comparing with other anchoring methods, enhanced the colonization of other organisms such as macroalgae and sessile invertebrates, contributing to generate a natural habitat.


2017 ◽  
Vol 139 (5) ◽  
Author(s):  
Sara Benyakhlef ◽  
Ahmed Al Mers ◽  
Ossama Merroun ◽  
Abdelfattah Bouatem ◽  
Hamid Ajdad ◽  
...  

Reducing levelized electricity costs of concentrated solar power (CSP) plants can be of great potential in accelerating the market penetration of these sustainable technologies. Linear Fresnel reflectors (LFRs) are one of these CSP technologies that may potentially contribute to such cost reduction. However, due to very little previous research, LFRs are considered as a low efficiency technology. In this type of solar collectors, there is a variety of design approaches when it comes to optimizing such systems. The present paper aims to tackle a new research axis based on variability study of heliostat curvature as an approach for optimizing small and large-scale LFRs. Numerical investigations based on a ray tracing model have demonstrated that LFR constructors should adopt a uniform curvature for small-scale LFRs and a variable curvature per row for large-scale LFRs. Better optical performances were obtained for LFRs regarding these adopted curvature types. An optimization approach based on the use of uniform heliostat curvature for small-scale LFRs has led to a system cost reduction by means of reducing its receiver surface and height.


2018 ◽  
Vol 20 (21) ◽  
pp. 4911-4919 ◽  
Author(s):  
Rahul Prasad Bangalore Ashok ◽  
Pekka Oinas ◽  
Kalle Lintinen ◽  
Golam Sarwar ◽  
Mauri A. Kostiainen ◽  
...  

Economical large-scale production of colloidal lignin particles.


Author(s):  
Reza Ziazi ◽  
Kasra Mohammadi ◽  
Navid Goudarzi

Hydrogen as a clean alternative energy carrier for the future is required to be produced through environmentally friendly approaches. Use of renewables such as wind energy for hydrogen production is an appealing way to securely sustain the worldwide trade energy systems. In this approach, wind turbines provide the electricity required for the electrolysis process to split the water into hydrogen and oxygen. The generated hydrogen can then be stored and utilized later for electricity generation via either a fuel cell or an internal combustion engine that turn a generator. In this study, techno-economic evaluation of hydrogen production by electrolysis using wind power investigated in a windy location, named Binaloud, located in north-east of Iran. Development of different large scale wind turbines with different rated capacity is evaluated in all selected locations. Moreover, different capacities of electrolytic for large scale hydrogen production is evaluated. Hydrogen production through wind energy can reduce the usage of unsustainable, financially unstable, and polluting fossil fuels that are becoming a major issue in large cities of Iran.


2014 ◽  
Vol 39 (1) ◽  
pp. 165-172
Author(s):  
Praveen Kumar Verma ◽  
SK Nag ◽  
SK Patil

The paper has studied the economic viability of improved technology (Introduced under NAIP component-3) for extraction of cashew kernel from cashew nut in Bastar region of Chhattisgarh, India. Cost concept has been used to calculate economics of cashew kernel. The technology (Boiling, steaming, cutting, drying, and peeling) has been found viable over conventional practices (Traditional manual separation by stone or hammer) on account of higher recovery of 40 percent and cost reduction by 29.71 percent. Overall net profit per unit (One unit includes one boiler, one steamer, two cutter, one dryer, six peelers and cost of land, depreciation and interest on working capital) in the case of improved technology has been estimated to be Rs 7.32 lakh. Cost of production in machine extraction practices was 202.80 Rupees per kilogram of cashew in spite of traditionally practiced 288.56 Rupees per kilogram. The cost benefit ratio was found higher in machine extraction (1.57) as compare to traditionally practiced (0.169). The mechanical decortications and separation could not only save time and money, also reduced women drudgery (due to manual breaking by stone or hammer to separate kernel). The technology has been found suitable for promotion of entrepreneurship on the processing of cashew kernel from cashew nut in the production catchments which otherwise is not properly utilized. DOI: http://dx.doi.org/10.3329/bjar.v39i1.20166 Bangladesh J. Agril. Res. 39(1): 165-172, March 2014


Author(s):  
Glenn McAndrews

Electric starter development programs have been the subject of ASME technical papers for over two decades. Offering significant advantages over hydraulic or pneumatic starters, electric starters are now poised to be the preferred choice amongst gas turbine customers. That they are not now the dominant starter in the field after decades of investment and experimentation is attributable to many factors. As with any new technology, progress is often unsteady, depending on budgets, market conditions, customer buy-in, etc. Additionally, technological advances in the parent technologies, in this case electric motors, can abruptly and rapidly change, further disturbing the best laid introduction plans. It is therefore not too surprising that only recently, is the industry beginning to see the deployment of electric starters on production gas turbines. The earliest adoption occurred on smaller gas turbine units, generally less than 10 MW in power. More recently, gas turbines greater than 10 MWs are being sold with electric starters. The authors expect that regardless of their size or fuel supply, most all future gas turbine users will opt for electric starters. This may even include the “larger” frame machines with power greater than 100 MW. Starting with some past history, this paper will not only summarize past development efforts, it will attempt to examine the current deployment of electric starters throughout the marine and industrial gas turbine landscapes. The large-scale acceptance of electric start systems for both new production and retrofit will depend on the favorable cost/benefit assessment when weighing both first cost and life cycle cost. The current and intense activity in electric vehicle applications is giving rise to even more power dense motors. The paper will look at some of these exciting applications, the installed products, and the technologies behind the products. To what extent these new products may serve the needs of the gas turbine community will be the central question this paper attempts to answer.


Author(s):  
Valentin Tablan ◽  
Ian Roberts ◽  
Hamish Cunningham ◽  
Kalina Bontcheva

Cloud computing is increasingly being regarded as a key enabler of the ‘democratization of science’, because on-demand, highly scalable cloud computing facilities enable researchers anywhere to carry out data-intensive experiments. In the context of natural language processing (NLP), algorithms tend to be complex, which makes their parallelization and deployment on cloud platforms a non-trivial task. This study presents a new, unique, cloud-based platform for large-scale NLP research—GATECloud. net. It enables researchers to carry out data-intensive NLP experiments by harnessing the vast, on-demand compute power of the Amazon cloud. Important infrastructural issues are dealt with by the platform, completely transparently for the researcher: load balancing, efficient data upload and storage, deployment on the virtual machines, security and fault tolerance. We also include a cost–benefit analysis and usage evaluation.


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