scholarly journals Comprehensive analysis of differences of N6-methyladenosine of lncRNAs between atrazine-induced and normal Xenopus laevis testis

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Xuejie Qi ◽  
Xiao Geng ◽  
Juan Zhang ◽  
Binpeng Qu ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Endocrine Disrupting Chemicals ◽  
Model Organism ◽  
Control Group ◽  
Ideal Model ◽  
Germline Development ◽  
Amphibian Species ◽  
Male Germline ◽  
Endocrine Disrupting ◽  
First Time

Abstract Background Increasing evidence suggested N6-methyladenosine (m6A) modification is crucial for male germline development. However, m6A modification of lncRNAs gains a little attention in amphibians in recent years. Xenopus laevis (X. laevis) was chosen to be an ideal model organism for testing environmental endocrine disrupting chemicals (EDCs) exposure and resultant effects. Atrazine (AZ) as an endocrine disrupt can effect development of testis in amphibians. Our previous study revealed that m6A is a highly conserved modification across the species. Results The results of m6A sequences showed that m6A-methylated lncRNAs enriched in intergenic region in testes of X. laevis. We further examined the differential expression of lncRNAs m6A sites in testes of AZ-exposed and compared with that in animals from control group. The results indicated that up to 198 differentially methylated m6A sites were detected within 188 lncRNAs, in which 89 significantly up-methylated sites and 109 significantly down-methylated sites. Data from KEGG pathway analysis indicated that AZ-affected lncRNAs m6A sites were mainly involved in 10 pathways in which 3 mutual pathways were found in the result of differentially m6A-methylated mRNAs. Conclusions These findings suggested that differentially m6A-methylated lncRNAs and these 3 pathways may act on regulatory roles in abnormal testis development of AZ-exposed X. laevis. This study for the first time provides insights into the profile of lncRNAs m6A modifications in amphibian species.

scholarly journals Comprehensive Analysis of Differences of N6-Methyladenosine of Lncrnas Between Atrazine-Inducedand Normal Xenopus Laevis Testis

2021 ◽  
Author(s):  
Xuejie Qi ◽  
Xiao Geng ◽  
Juan Zhang ◽  
Ling Li ◽  
Qiang Jia ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Rna Stability ◽  
Enrichment Analysis ◽  
Control Group ◽  
Pathway Enrichment Analysis ◽  
Amphibian Species ◽  
Illumina Hiseq ◽  
Kegg Pathways ◽  
Non Coding Rna ◽  
First Time

Abstract Background: Increasing evidence suggested N6-methyladenosine (m6A) plays an important role in RNA stability, degradation, splicing and translation. M6A is found in different RNA including long non-coding RNA (lncRNA) which has been found possess significant biological functions. Our previous study examined the m6A profile of mRNAs in testis tissues of Xenopus laevis (X. laevis) with and without treatment with 100 µg/L atrazine (AZ). The result revealed that m6A is a highly conserved modification across the species. Methods: In this study, we apply previous approach to further investigate m6A modification profile of lncRNAs and predict the potential mechanism. In brief, m6A was sequenced by MeRIP sequencing using the latest Illumina HiSeq sequencer. Pathway enrichment analysis was used to maps genes to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Results: The results showed that m6A of lncRNAs enriched around intergenic region in testes of X. laevis. We further investigated the differential expression of lncRNAs m6A in testes of AZ-exposed compared with that in animals from control group. The results indicated that up to 198 differentially methylated m6A sites were detected within 188 lncRNAs, in which 89 sites were significantly up-regulated and 109 sites were significantly down-regulated. Data from Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that AZ-affected lncRNAs m6A sites were mainly involved in 10 pathways in which 3 mutual pathways were found in the result of differentially m6A-methylated mRNAs. Conclusions: These findings suggest that differentially m6A-methylated lncRNAs and these 3 pathways may act on regulatory roles in abnormal testis development of AZ-exposed X. laevis. This study for the first time provide insights into the profile of lncRNAs m6A modifications in amphibian species.

scholarly journals OctylPhenol (OP) Alone and in Combination with NonylPhenol (NP) Alters the Structure and the Function of Thyroid Gland of the Lizard Podarcis siculus

2021 ◽  
Vol 80 (3) ◽  
pp. 567-578 ◽  
Author(s):  
Rosaria Sciarrillo ◽  
Mariana Di Lorenzo ◽  
Salvatore Valiante ◽  
Luigi Rosati ◽  
Maria De Falco
Keyword(s):  
Thyroid Gland ◽  
Endocrine Disrupting Chemicals ◽  
Thyroid Stimulating Hormone ◽  
Control Group ◽  
Type Ii ◽  
Endocrine Disrupting ◽  
Pituitary Thyroid Axis ◽  
Podarcis Siculus ◽  
Thyroid Axis ◽  
By Products

Abstract Different environmental contaminants disturb the thyroid system at many levels. AlkylPhenols (APs), by-products of microbial degradation of AlkylPhenol Polyethoxylates (APEOs), constitute an important class of Endocrine Disrupting Chemicals (EDCs), the two most often used environmental APs being 4-nonylphenol (4-NP) and 4-tert-octylphenol (4-t-OP). The purpose of the present study was to investigate the effects on the thyroid gland of the bioindicator Podarcis siculus of OP alone and in combination with NP. We used radioimmunoassay to determine their effects on plasma 3,3′,5-triiodo-L-thyronine (T3), 3,3′,5,5′-L-thyroxine (T4), thyroid-stimulating hormone (TSH), and thyrotropin-releasing hormone (TRH) levels in adult male lizards. We also investigated the impacts of AP treatments on hepatic 5′ORD (type II) deiodinase and hepatic content of T3 and T4. After OP and OP + NP administration, TRH levels increased, whereas TSH, T3, and T4 levels decreased. Lizards treated with OP and OP + NP had a higher concentration of T3 in the liver and 5′ORD (type II) activity, whereas T4 concentrations were lower than that observed in the control group. Moreover, histological examination showed that the volume of the thyroid follicles became smaller in treated lizards suggesting that that thyroid follicular epithelial cells were not functionally active following treatment. This data collectively suggest a severe interference with hypothalamus–pituitary–thyroid axis and a systemic imbalance of thyroid hormones. Graphic Abstract

Engaging Undergraduates in the Scientific Process: Exploring Invertebrate Endocrine Disruption

2016 ◽  
Vol 78 (5) ◽  
pp. 410-416 ◽  
Author(s):  
Scott Layton ◽  
Jason Belden
Keyword(s):  
Endocrine Disruption ◽  
Endocrine Disrupting Chemicals ◽  
Model Organism ◽  
Female Offspring ◽  
Reproduction Rate ◽  
Interest In Science ◽  
Endocrine Disrupting ◽  
Set Up ◽  
Reproductive Study ◽  
Analyze Data

Engaging students in the process of science to increase learning and critical thinking has become a key emphasis in undergraduate education. Introducing environmental topics, such as the effects of endocrine-disrupting chemicals, into undergraduate courses offers a new means to increase student engagement. Daphnia magna can serve as a model organism for endocrine disruption, and its ease of handling, rapid reproduction rate, and clearly defined endpoints make it useful in short-term, student research projects. The concept of endocrine disruption can be tested through a 21-day reproductive study of D. magna exposed to varying concentrations of the pesticide fenoxycarb. Students will observe an altered reproduction rate and increased production of males under conditions that would typically result only in the production of female offspring. This research system allows students to formulate hypotheses, set up experiments, analyze data, and present results, leading to a greater appreciation of and interest in science.

The effects of UV-B radiation and endocrine-disrupting chemicals (EDCs) on the biology of amphibians

2001 ◽  
Vol 9 (2) ◽  
pp. 61-80 ◽  
Author(s):  
D Crump
Keyword(s):  
Meta Analysis ◽  
Endocrine Disrupting Chemicals ◽  
Reproductive Toxicity ◽  
Abnormal Development ◽  
Data Sets ◽  
Population Declines ◽  
Amphibian Species ◽  
Endocrine Disrupting ◽  
Polycyclic Aromatic ◽  
The 1960S

Statistical meta-analysis of large and diverse data sets has indicated that amphibians have been declining worldwide since the 1960s. Exposure to UV-B radiation (280–320 nm) and endocrine-disrupting chemicals (EDCs) have been considered as possible hypotheses to explain the observed declines. Equivocal conclusions have been reached with respect to the effects of UV-B on amphibian populations. Field and laboratory studies employing both ecologically relevant and enhanced UV-B levels have been conducted using a variety of amphibian species and reports differ with respect to the most sensitive developmental stage and the ultimate implications. UV-B radiation has also been shown to interact with other stressors (e.g., pesticides, polycyclic aromatic hydrocarbons, low pH) resulting in decreased survivorship for several amphibian species. Limited evidence of reproductive toxicity of xenobiotics in amphibians exist; however, early exposure to EDCs could cause abnormal development of the amphibian reproductive system, inhibit vital hormone messages that drive metamorphosis, and ultimately contribute to the decline of some amphibian populations. The available evidence suggests that more than one agent is contributing to amphibian population declines and the following review narrows the focus to address the existing data on the effects of UV-B, alone and in combination with other stressors, and EDCs on amphibian survivorship and development. Key words: amphibians, UV-B radiation, endocrine-disrupting chemicals, declines, review.

scholarly journals Development and Effect of a Behavior Program for Reducing Exposure to Endocrine Disrupting Chemicals in Unmarried Women

2021 ◽  
Vol 25 (1) ◽  
pp. 63-72
Author(s):  
Hye Jin Kim ◽  
So Young Choi
Keyword(s):  
Self Efficacy ◽  
Endocrine Disrupting Chemicals ◽  
Nursing Intervention ◽  
Perceived Barriers ◽  
Control Group ◽  
Perceived Benefits ◽  
Health Promotion Model ◽  
Face To Face ◽  
Endocrine Disrupting ◽  
Pender's Health Promotion Model

Purpose: This study was conducted to develop a behavior program for reducing exposure to endocrine disrupting chemicals (EDCs), thereby to improve perceived benefits and self-efficacy as well as reduce the perceived barriers and prevalence of dysmenorrhea in unmarried women.Methods: The study used a nonequivalent control group pretest-posttest design. Participants were randomly assigned to an experiment (n=18) or control group (n=18). The behavior program for reducing exposure to EDCs was developed based on Pender's health promotion model and previous research results. The program was conducted for 12 weeks, consisting of 4 weeks of face-to-face intervention and 8 weeks of mobile intervention.Results: The behavior program we developed was verified as an effective intervention that could improve the perceived benefits and self-efficacy of reducing one's exposure to EDCs. It also decreases the perceived barriers and prevalence of dysmenorrhea in unmarried women.Conclusion: Our results showed that the program was an effective nursing intervention in unmarried women. Therefore, this behavior program for reducing exposure to EDCs can be applied in the context of unmarried women.

150 IN VITRO MODEL FOR EFFECT OF ENDOCRINE-DISRUPTING CHEMICALS ON PLACENTAL CATION TRANSPORTER CHANNEL

2016 ◽  
Vol 28 (2) ◽  
pp. 205
Author(s):  
J.-H. Lee ◽  
M. H. Lee ◽  
M. J. Lee ◽  
E.-B. Jeung
Keyword(s):  
Cell Line ◽  
Steroid Hormones ◽  
Endocrine Disrupting Chemicals ◽  
Control Group ◽  
Bewo Cell ◽  
Mrna Levels ◽  
Phenol Red ◽  
Human Trophoblast ◽  
Protein Levels ◽  
Endocrine Disrupting

Calcium, copper, iron, oxygen, and carbon dioxide are essential factors in fetal growth. These molecules are transferred by specific receptors located on the cell membrane or cytoplasm in placenta. Calcium, copper, and iron transfer genes are regulated by oestrogen, placental lactogen, and vitamin D. During pregnancy, expression of these receptors is controlled by the nutritional status of the maternal and fetal environment. Some synthetic plastics contain endocrine-disrupting chemicals (EDC), which have similar structures to steroid hormones or endogenous hormones related to reproduction. These substances disturb action of hormones (e.g. increasing oestrogen or progesterone) by interacting with their receptors or affecting the expression of transporting genes for cations. We used a BeWo cell line (human trophoblast cell line) to test the effect of EDC during pregnancy. The cells were cultured in phenol red-free DMEM supplemented with 5% charcoal dextran-stripped fetal bovine serum for 48 h to ensure the depletion of steroid hormones in the cells. Ethinyl oestradiol (EE), which activates oestrogen receptors, was used as a positive control. Then, EE (10–9, 10–8, and 10–7 M), octylpehnol (OP; 10–7, 10–6, and 10–5 M), nonylphenol (NP; 10–8, 10–7, and 10–6 M), and bisphenol A (BPA; 10–7, 10–6, and 10–5 M) were treated in BeWo cells for 48 h, and the cells were harvested. The mRNA and protein levels for calcium transporting genes (PMCA1 and TRPV6), copper transporting genes (CTR1 and ATP7A), and iron transporting genes (IREG1 and HEPH) were quantified by RT-qPCR, and Western blotting, respectively. Experiments were carried 3 times, and results were statistically analysed by GraphPad Prism6 (GraphPad Software, San Diego, CA, USA). We observed dose-dependent decreases in mRNA levels of PMCA1, TRPV6, ATP7A, and IREG1 compared with control group in OP-, NP-, or BPA-treated groups. Protein levels showed a similar pattern to mRNA levels. Based on our data, we confirmed that these EDC affect metal ion channels such as calcium, copper, and iron transporters during pregnancy.

scholarly journals Paternal Occupational Exposure to Endocrine-Disrupting Chemicals as a Risk Factor for Leukaemia in Children: A Case-Control Study from the North of England

2014 ◽  
Vol 2014 ◽  
pp. 1-7
Author(s):  
Mark S. Pearce ◽  
Lucy R. Baxter ◽  
Louise Parker
Keyword(s):  
Occupational Exposure ◽  
Case Control Study ◽  
Endocrine Disrupting Chemicals ◽  
Control Group ◽  
Live Births ◽  
The North ◽  
Increased Risk ◽  
Endocrine Disrupting ◽  
Control Study ◽  
Polychlorinated Organic Compounds

Occupations with exposures to a variety of chemicals, including those thought to be potential endocrine disruptors, have been associated with an increased risk of leukaemia in offspring. We investigated whether an association exists between paternal occupations at birth involving such exposures and risk of leukaemia in offspring. Cases (n=958) were matched, on sex and year of birth, to controls from two independent sources, one other cancers, one cancer-free live births. Paternal occupations at birth were classified, using an occupational exposure matrix, as having “very unlikely,” “possible,” or “likely” exposure to six groups of potential endocrine-disrupting chemicals. There was a significantly increased risk of acute nonlymphocytic leukaemia (ANLL) for polychlorinated organic compounds (OR 1.95, 95% CI 1.08–3.54) only in comparison with cancer-free controls, and for phthalates (OR 1.61, 95% CI 1.00–2.61) only with registry controls. A number of other, including inverse, associations were seen, but limited to one control group only. No associations were seen with likely paternal exposure to heavy metals. The associations identified in this study require further investigation, with better exposure and potential confounding (for example maternal variables) information, to evaluate the likelihood of true associations to assess whether they are real or due to chance.

Sign in / Sign up

Export Citation Format

Share Document