scholarly journals Antioxidant capacity of Nitraria retusa leaf extracts against mitomycin C-induced genetic toxicity in male mice

2019 ◽  
Vol 80 (1) ◽  
Author(s):  
Wagdy K. B. Khalil ◽  
Ekram S. Ahmed ◽  
Salwa M. Kassem ◽  
Thanaa M. T. Shoman ◽  
Mahrousa M. Hassanane ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Mitomycin C ◽  
Male Mice ◽  
Leaf Extracts ◽  
Genetic Toxicity ◽  
Nitraria Retusa

scholarly journals Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4041
Author(s):  
Adriana Cecilia Csakvari ◽  
Cristian Moisa ◽  
Dana G. Radu ◽  
Leonard M. Olariu ◽  
Andreea I. Lupitu ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Antioxidant Capacity ◽  
Cannabis Sativa ◽  
Antibacterial Properties ◽  
Green Building ◽  
Human Pathogens ◽  
Leaf Extracts ◽  
Vis Spectroscopy ◽  
Ft Ir

Cannabis sativa L. (hemp) is a plant used in the textile industry and green building material industry, as well as for the phytoremediation of soil, medical treatments, and supplementary food products. The synergistic effect of terpenes, flavonoids, and cannabinoids in hemp extracts may mediate the biogenic synthesis of metal nanoparticles. In this study, the chemical composition of aqueous leaf extracts of three varieties of Romanian hemp (two monoecious, and one dioecious) have been determined by Fourier-Transformed Infrared spectroscopy (FT-IR), high-performance liquid chromatography, and mass spectrometry (UHPLC-DAD-MS). Then, their capability to mediate the green synthesis of silver nanoparticles (AgNPs) and their pottential antibacterial applications were evaluated. The average antioxidant capacity of the extracts had 18.4 ± 3.9% inhibition determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 78.2 ± 4.1% determined by 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS™) assays. The total polyphenolic content of the extracts was 1642 ± 32 mg gallic acid equivalent (GAE) L−1. After this, these extracts were reacted with an aqueous solution of AgNO3 resulting in AgNPs, which were characterized by UV−VIS spectroscopy, FT-IR, scanning electron microscopy (SEM-EDX), and dynamic light scattering (DLS). The results demonstrated obtaining spherical, stable AgNPs with a diameter of less than 69 nm and an absorbance peak at 435 nm. The mixture of extracts and AgNPs showed a superior antioxidant capacity of 2.3 ± 0.4% inhibition determined by the DPPH• assay, 88.5 ± 0.9% inhibition as determined by the ABTS•+ assay, and a good antibacterial activity against several human pathogens: Escherichia coli, Klebsiella pneumoniae, Pseudomonas fluorescens, and Staphylococcus aureus.

Mutagenic, antimutagenic and antioxidant potency of leaf extracts from Nitraria retusa

2010 ◽  
Vol 48 (8-9) ◽  
pp. 2283-2290 ◽  
Author(s):  
Jihed Boubaker ◽  
Ines Skandrani ◽  
Ines Bouhlel ◽  
Mohamed Ben sghaier ◽  
Aicha Neffati ◽  
...  
Keyword(s):  
Leaf Extracts ◽  
Nitraria Retusa ◽  
Antioxidant Potency

Antioxidant activity and zebrafish teratogenicity of hydroalcoholic Moringa oleifera L. leaf extracts

2020 ◽  
Vol 122 (10) ◽  
pp. 3129-3137
Author(s):  
Nurul Fatin Syazleen Mohamad Shariff ◽  
Thangam Singgampalam ◽  
Chean Hui Ng ◽  
Chin Siang Kue
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Moringa Oleifera ◽  
Zebrafish Embryo ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Aqueous Methanol ◽  
Leaf Extracts ◽  
Content Type ◽  
Aqueous Ethanolic Extract

PurposeMoringa oleifera (MO) is a herbal remedy that is rich in essential amino acids and phytochemicals in the leaves, seeds and pods. It is becoming a famous food in various ethnic groups, and the consumption is increasing worldwide. However, there is no safety profile of the hydroalcoholic MO leaf extracts, or documented teratogenicity aspect. This study was sought to compare the antioxidant activity and the teratogenicity of hydroalcoholic MO leaf extracts in the zebrafish embryo.Design/methodology/approachMO leaves were extracted with aqueous alcohols (ethanol or methanol: water, 80:20 v/v) using the Soxhlet method. The antioxidant capacity was determined using DPPH assay. The concentrations of extracts ranging from 3 to 1,000 µg/mL were used to examine the toxicity and teratogenicity on the zebrafish embryo.FindingsBoth hydroalcoholic MO leaf extracts were positive for alkaloids, terpenoids, steroids, tannins, flavonoids, phenols, coumarins, quinones and glycosides, except saponins only absent in aqueous methanol extract. The antioxidant capacity based on lC50, was 15.92 ± 3.62 mg/mL for aqueous methanolic and 25.28 ± 2.89 mg/mL for aqueous ethanolic extract. For acute toxicity, the aqueous methanolic extract has lower LC50 (163.87 ± 12.88 µg/mL) compared to aqueous ethanolic extract (337.48 ± 30.04 µg/mL). The embryo treated with aqueous methanolic extract showed phenotypic defect but not on the aqueous ethanolic extract.Originality/valueThis study suggests that aqueous methanol of MO leaves extract has better antioxidant capacity compared to ethanol, and ethanolic leaf extract is safer (higher LC50 and no teratogenicity) than methanolic extract.

The Antioxidant Effects of Germinating Tartary Buckwheat Vinegar

2014 ◽  
Vol 651-653 ◽  
pp. 305-312 ◽  
Author(s):  
Yun Long Li ◽  
Ting Jun Ma ◽  
Hong Mei Li ◽  
Jun Jun Hu ◽  
Jun Sheng Bian ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Dose Group ◽  
Low Dose ◽  
Positive Control ◽  
Control Model ◽  
Male Mice ◽  
Total Antioxidant ◽  
Antioxidant Effects ◽  
Different Doses

The present study was to evaluated the antioxidant capacity and the effect to the D - galactose senile mice of buckwheat vinegar which maked by sprouting buckwheat, divided healthy Kunming male mice into blank, positive control, model and three dose groups of vinega made by stirring buckwheat randomly. After 6 weeks, it was determined the activity of antioxidant enzyme and malondialdehyde in mice with different doses groups. The results showed that: the stirring buckwheat vinegar exhibited strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50 was 0.58*10-2 ) and total antioxidant capacity (210.58 mmolFe2SO4•ml-1 ); the vinegar can droped the level of MDA and improved the activity of SOD in liver of D - galactose senile mice. The low-dose group can improved the activity of SOD in serum. However, for the aging mice, the buckwheat vinegar had no effect on behavior when they were in the new environment, the ability of reduced the activity of TChE in brain and the level of MDA in serum was not significant, at the same time, the activity of GSH-px in the serum couldn't be enhanced.

scholarly journals Transgenic Production of Cytokinin Suppresses Bacterially Induced Hypersensitive Response Symptoms and Increases Antioxidative Enzyme Levels in Nicotiana spp.

2008 ◽  
Vol 98 (11) ◽  
pp. 1242-1247 ◽  
Author(s):  
B. Barna ◽  
A. C. Smigocki ◽  
J. C. Baker
Keyword(s):  
Antioxidant Capacity ◽  
Pseudomonas Syringae ◽  
Hypersensitive Response ◽  
Small Subunit ◽  
Membrane Lipid ◽  
Molar Ratio ◽  
Leaf Extracts ◽  
Free Sterols ◽  
Proteinase Inhibitor Ii ◽  
Pseudomonas Syringae Pathovars

Responses of cytokinin overproducing transgenic Nicotiana plants to infections with compatible and incompatible Pseudomonas syringae pathovars were compared. Plants used were transformed with the ipt(isopentenyl transferase) gene that catalyzes the synthesis of cytokinin. In cytokinin overproducing lines that carry the ipt gene fused to the CaMV 35S (Nt+ipt), the wound-inducible proteinase inhibitor II (Ntx+ipt), or the light-inducible Rubisco small subunit protein (Npl+ipt) promoter, development of the hypersensitive response (HR) after infection with incompatible bacteria (P. syringae pv. tomato) was significantly inhibited as compared to the untransformed (Nt) controls. Over a 12 h period following inoculation, P. syrinage pv. tomato populations were slightly reduced in leaves of the cytokinin-overproducing Nt-ipt line compared with the Nt control. When the compatible P. syringae. pv. tabaci was used to infect the ipt transformed lines, slight or no significant differences in necrosis development were observed. Following infection, the titer of P. syringae pv. tabaci increased rapidly in both the transgenic and control lines but was higher in Nt+ipt plants. Leaf superoxide dismutase and catalase enzyme activities were about 60% higher in ipt leaf extracts than in the controls. This augmented antioxidant capacity likely decreased the amount of H2O2 that may be associated with the higher tolerance of plants to pathogen-induced necrosis. In addition, the Nt+ipt lines had a significantly lower molar ratio of free sterols to phospholipids. The more stable membrane lipid composition and the higher antioxidant capacity likely contributed to the suppressed HR symptoms in the cytokinin overproducing Nt+ipt plants. In conclusion, the overproduction of cytokinins in tobacco appears to suppress the HR symptoms induced by incompatible bacteria.

Phenolic Profile, Antioxidant Capacity, and Antimicrobial Activity of Leaf Extracts from SixVitis viniferaL. Varieties

2012 ◽  
Vol 16 (1) ◽  
pp. 45-60 ◽  
Author(s):  
Višnja Katalinic ◽  
Sonja Smole Mozina ◽  
Ivana Generalic ◽  
Danijela Skroza ◽  
Ivica Ljubenkov ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Capacity ◽  
Leaf Extracts ◽  
Phenolic Profile

Combined Therapy of Mitomycin C and Chinese Medical Herbs on Experimental Liver Cancer

1993 ◽  
Vol 21 (01) ◽  
pp. 51-58 ◽  
Author(s):  
Dou-Mong Hau ◽  
Tai-Hei Chen ◽  
Jau-Forng Cheng ◽  
Jyu-Sheng You
Keyword(s):  
Mortality Rate ◽  
Liver Cancer ◽  
Mitomycin C ◽  
Combined Therapy ◽  
Tumor Control ◽  
Sarcoma 180 ◽  
Male Mice ◽  
Mean Survival Time ◽  
The Mean ◽  
Experimental Liver

In this research, ICR male mice were chosen for intrahepatic implantation of sarcoma 180 tumor cells (1 × 107). The mice were randomly divided into various groups 24 hours after implantation. One of the groups was the tumor control, the others were singly or combinedly treated with mitomycin C (MMC) and Shih Chuan-Ta-Pu-Tang (SCTPT) or Shi-Hung-One (SHO). The results revealed that the mortality rate (MR60) in the tumor control was 100% and the mean survival time (MST60) was 21.11 ± 10.69 days. The best therapeutic effect appeared in the group treated with the combination of MMC and SHO, its MR6o was 55.0% and MST 60 was 47.0 ± 13.4 days.

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