Ascitic interleukin-12 is an independent prognostic factor in ovarian cancer.

1998 ◽  
Vol 16 (5) ◽  
pp. 1861-1868 ◽  
Author(s):  
A G Zeimet ◽  
M Widschwendter ◽  
C Knabbe ◽  
D Fuchs ◽  
M Herold ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Immune Response ◽  
Ascitic Fluid ◽  
Ascites Fluid ◽  
Interleukin 12 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Stepwise Logistic Regression ◽  
Cox Regression Analysis ◽  
Cytokine Levels ◽  
Disease Free

PURPOSE The clinical impact of endogenous cytokines supplied with deterministic properties in the generation of either T helper (Th)1 -type or Th2-type immune response was investigated in patients with ovarian cancer. Whereas interleukin (IL)- 12 initiates the differentiation of naive Th0 cells toward Th1 phenotype, IL-4 and IL-10 mediate the development of Th2-type immunity. PATIENTS AND METHODS Cytokines were determined before treatment by means of enzyme-linked immunosorbent assay (ELISA) in ascites fluid and serum of 76 patients with ovarian cancer. Cytokine levels were compared with each other and with standard clinicopathologic parameters. A stepwise logistic regression was calculated to rule out interdependence in the associations of the various variables. Survival analyses were performed with the Kaplan-Meier method and differences in survival were examined according to Mantel and Breslow. Cox proportional hazards analysis was used to identify independent prognostic factors. RESULTS Whereas IL-10 and IL-12 were detectable in all ascites-fluid samples, IL-4 was measurable in only 43% of the specimens. With the exception of neopterin, macrophage colony-stimulating factor (M-CSF), and IL-4, determined cytokine levels were significantly elevated in ascites fluid compared with serum (P < .01). In univariate analyses, high ascitic-fluid concentrations of either neopterin, tumor necrosis factor-alpha (TNF-alpha), or IL-12 were associated with poor disease-free (P < .005) and overall (P < .01) survival. Multivariate Cox regression analysis showed ascitic-fluid IL-12 levels to be the only immunologic variable that retained independent prognostic significance (P < .03 for disease-free and P < .01 for overall survival), together with residual disease, Fédération Internationale de Gynécologie et d'Obstétrique (FIGO)-stage, and patient age. CONCLUSION In ovarian cancer, high ascitic-fluid IL-12 levels, which may indicate a local Th1-generated immune response, are associated with disease progression.

Effects of Interleukin-12 on the Immune Response to a Multipeptide Vaccine for Resected Metastatic Melanoma

2001 ◽  
Vol 19 (18) ◽  
pp. 3836-3847 ◽  
Author(s):  
P. Lee ◽  
F. Wang ◽  
J. Kuniyoshi ◽  
V. Rubio ◽  
T. Stuge ◽  
...  
Keyword(s):  
Immune Response ◽  
High Risk ◽  
Immune Responses ◽  
Mononuclear Cells ◽  
Peptide Vaccine ◽  
Significant Proportion ◽  
Interleukin 12 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Skin ◽  
Before And After

PURPOSE: Forty-eight patients with high-risk re-sected stage III or IV melanoma were immunized with two tumor antigen epitope peptides derived from gp100209-217(210M) (IMDQVPSFV) and tyrosin-ase368-376(370D) (YMDGTMSQV) emulsified with incomplete Freund’s adjuvant (IFA). Patients received peptides/IFA with or without interleukin (IL)-12 30 ng/kg to evaluate the toxicities and immune responses in either arm with time to relapse and survival as secondary end points. PATIENTS AND METHODS: Immunizations were administered every 2 weeks for 8 weeks, then every 4 weeks for 12 weeks, and then once 8 weeks later. A leukapheresis to obtain peripheral-blood mononuclear cells for immune analyses was done before and after vaccination. Skin testing with peptides and recall reagents was performed before and after vaccinations. RESULTS: Local pain and granuloma formation, fever, and lethargy of grade 1 or 2 were observed. Transient vaccine-related grade 3—but no grade 4—toxicity was observed. Thirty-four of 40 patients developed a positive skin test response to the gp100 peptide but none to tyrosinase. Immune responses were measured by release of gamma-interferon in an enzyme-linked immunosorbent assay (ELISA) by effector cells in the presence of peptide-pulsed antigen-presenting cells or by an antigen-specific tetramer flow cytometry assay. Thirty-three of 38 patients demonstrated an immune response by ELISA after vaccination, as did 37 of 42 patients by tetramer assay. Twenty-four of 48 patients relapsed with a median follow-up of 20 months, and 10 patients in this high-risk group have died. CONCLUSION: These data suggest a significant proportion of patients with resected melanoma mount an antigen-specific immune response against a peptide vaccine and indicate that IL-12 may increase the immune response and supporting further development of IL-12 as a vaccine adjuvant.

scholarly journals Cytokine Profiles of AIDS Patients Are Similar to Those of Mice with Disseminated Cryptococcus neoformansInfection

1999 ◽  
Vol 67 (12) ◽  
pp. 6314-6320 ◽  
Author(s):  
Olivier Lortholary ◽  
Luce Improvisi ◽  
Naima Rayhane ◽  
Francoise Gray ◽  
Catherine Fitting ◽  
...  
Keyword(s):  
Immune Response ◽  
Murine Model ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cerebral Lesions ◽  
Aids Patients ◽  
Fungal Burden ◽  
Meningeal Involvement ◽  
Tnf Α ◽  
Cytokine Levels ◽  
The Brain

ABSTRACT Cryptococcosis is an hematogenously disseminated meningoencephalitis during which the relationship between the disease severity and the immune response remains unclear. We thus analyzed, by enzyme-linked immunosorbent assay, proinflammatory (tumor necrosis factor alpha [TNF-α] and interleukin-6 [IL-6]) and anti-inflammatory (IL-10) cytokine levels in plasma at the time of diagnosis in 51 AIDS patients with culture-proven cryptococcosis. We used a murine model to determine the correlation between cytokine levels and fungal burden in blood and tissues and the kinetics of the immune response and of the formation of cerebral lesions. In AIDS patients, plasma TNF-α and IL-10, but not IL-6, levels were significantly higher in the case of fungemia or disseminated infection than in their absence, whereas the presence of meningitis had no influence on these levels. In mice, none of these cytokines were detected within the first day after inoculation. Later on, TNF-α and IL-10, but not IL-6, levels in plasma correlated significantly with the fungal burden in the blood and spleen but not the brain. In the brain, cytokine levels were low compared to those in other compartments, and tissue lesions and a degree of infection similar to those observed in humans were seen, further suggesting the relevance of this experimental model. Thus, AIDS patients with cryptococcosis produce an immune response that reflects the dissemination but not the meningeal involvement. This murine model of disseminated cryptococcosis can be used to investigate the pathophysiology of cryptococcosis and new therapeutic approaches.

scholarly journals ART Regimen and Other Sociodemographics Do Not Affect Cytokine Expression in HIV Patients in Ghana

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Samuel Essien-Baidoo ◽  
Dorcas Obiri-Yeboah ◽  
Yeboah Kwaku Opoku ◽  
Elvis Ayamga ◽  
Kevin Hodi Zie ◽  
...  
Keyword(s):  
Immune System ◽  
Interleukin 12 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Interleukin 17 ◽  
Positive Trend ◽  
Second Line ◽  
Hiv Patients ◽  
Significant Difference ◽  
Cytokine Levels ◽  
The Mean

Background. HIV infection is marked by the production of cytokines by infected cells and cells of the immune system. Variations in the levels of cytokine in HIV-infected individuals significantly impact the role of the immune system with the possibility to affect the course of HIV disease by either exacerbating or suppressing HIV replication. Aim. The study sought to investigate the effect of sociodemographic indices, clinical laboratory parameters, and ART regimen on Th1, Th2, and Th17 cytokines in HIV patients. Materials and methods. A total of two hundred (200) HIV patients on either the first or second line of ART were recruited into the study. Sociodemographic indices were collected using researcher-administered questionnaires. Serum concentrations of two major immune-promoting cytokines, IL-12 and IFN-γ, and immune-suppressive cytokines, IL-10 and IL-17, were measured using enzyme-linked immunosorbent assay (ELISA). T-test and chi-square were used to compare mean scores, while correlation (Pearson’s correlation) and linear regression analyses were also performed with the statistical significance set at p<0.05. Results. The mean age of the participants was (45.54 ± 0.7846) years with a greater proportion (84.5%) between 31 and 60 years. The mean interferon-gamma (INF-γ), interleukin- (IL-) 10, interleukin-12, and interleukin-17 were estimated to be 349.9 ± 8.391 pg/ml, 19.32 ± 0.4593 pg/ml, 19.23 ± 0.3960 pg/ml, and 24.6 ± 0.6207 pg/ml, respectively. Although INF-γ and IL-17 levels were relatively higher in males compared to females, it was vice versa for IL-10 and IL-12. However, none of these was statistically significant. Again, no significant difference was observed among all the cytokines stratified by the duration of ART, stage of HIV, and smoking status. Most importantly, stratification by either first- or second-line ART regimens recorded no significant difference in cytokine levels. Age significantly correlated inversely with IFN-γ (r = −0.27, p≤0.001), IL-10 (r = −0.24, p≤0.001), and IL-12 (r = −0.18, p=0.01) while duration on ART significantly correlated inversely with IFN-γ (r = −0.16, p=0.02). CD4 counts at 6 months and 12 months on ART correlated inversely with IL-17 (r = −0.17, p=0.02) and plasma viral load at 1 year (r = −0.22, p≤0.001), respectively. A positive correlation was observed between IFN-γ and IL-12 (r = −0.84, p≤0.001) and IL-17 (r = −0.50, p≤0.001). This positive trend was repeated between IL-10 and IL-12 (r = −0.92, p≤0.001) and IL-17 (r = −0.61, p≤0.001). Conclusion. The levels of IFN-γ, IL-12, IL-17, and IL-10 are not significantly affected by sociodemographics and ART regimen. This observation shows that no significant difference was observed in cytokine levels stratified by ART regiments. This means that both regimens are effective in the suppression of disease progression.

Clinical and Scintigraphic Findings in Radiation Myositis Complicating the Intraperitoneal Administration of 198Au

1965 ◽  
Vol 05 (02) ◽  
pp. 188-196
Author(s):  
J. Němec ◽  
J. Kubalt ◽  
S. Vohnout ◽  
J. Schubert ◽  
J. Sudek ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Ascitic Fluid ◽  
Saline Solution ◽  
Intraperitoneal Administration ◽  
Clinical Findings ◽  
Safety Measures ◽  
Muscle Necrosis

SummaryPraeperitoneal administration of colloidal 198Au complicating the treatment of anascitic ovarian cancer is reported. Typical scintiscans and various clinical findings of muscle necrosis and radiation myositis are described. Administration of larger amount of saline solution preceding the radiogold instillation controlled by simultaneous scintiscanning are suggested as safety measures in patients when no ascitic fluid is present.

Serum and ascites fluid cytokine levels in patients with chronic heart failure

2001 ◽  
Vol 56 (4) ◽  
pp. 219-223 ◽  
Author(s):  
Ramazan SARI ◽  
Bulent YILDIRIM ◽  
Alper SEVINC ◽  
Funda BAHCECI ◽  
Ramazan OZDEMIR ◽  
...  
Keyword(s):  
Heart Failure ◽  
Chronic Heart Failure ◽  
Ascites Fluid ◽  
Cytokine Levels

Ovarian Cancer: Differentially Expressed microRNAs in Tumor Tissue and Cell-Free Ascitic Fluid as Potential Novel Biomarkers

2019 ◽  
Vol 37 (9) ◽  
pp. 440-452 ◽  
Author(s):  
Luděk Záveský ◽  
Eva Jandáková ◽  
Vít Weinberger ◽  
Luboš Minář ◽  
Veronika Hanzíková ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Ascitic Fluid ◽  
Tumor Tissue ◽  
Differentially Expressed ◽  
Novel Biomarkers

scholarly journals A Novel Automated Immunoassay Platform to Evaluate the Association of Adiponectin and Leptin Levels with Breast Cancer Risk

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3303
Author(s):  
Debora Macis ◽  
Valentina Aristarco ◽  
Harriet Johansson ◽  
Aliana Guerrieri-Gonzaga ◽  
Sara Raimondi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Risk ◽  
Cancer Risk ◽  
Cox Model ◽  
Disease Free Survival ◽  
Postmenopausal Breast Cancer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Baseline Serum ◽  
Free Survival ◽  
Disease Free

Adiponectin and leptin are adipokines secreted by the adipose tissue that are associated with several chronic diseases including cancer. We aimed to compare the immunoassay platform ELLA with an enzyme-linked immunosorbent assay (ELISA) kit and to assess whether the results of the association analyses with breast cancer risk were dependent on the assay used. We measured adiponectin and leptin with ELLA and ELISA on baseline serum samples of 116 Italian postmenopausal women enrolled in two international breast cancer prevention trials. Results were compared with Deming, Passing–Bablok regression and Bland–Altman plots. Disease-free survival was analyzed with the Cox model. There was a good correlation between the methods for adiponectin and leptin (r > 0.96). We found an increased breast cancer risk for very low adiponectin levels (HR for ELLA = 3.75; 95% CI: 1.37;10.25, p = 0.01), whereas no significant association was found for leptin levels. The disease-free survival curves were almost identical for values obtained with the two methods, for both biomarkers. The ELLA platform showed a good concordance with ELISA for adiponectin and leptin measurements. Our results support the association of very low adiponectin levels with postmenopausal breast cancer risk, irrespective of the method used. The ELLA platform is a time-saving system with high reproducibility, therefore we recommend its use for biomarker assessment.

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