Nickel-Low Diet Formulation and Tissue Nickel Measurement

2020 ◽  
pp. 355-364
Author(s):  
Eric O. Uthus
Keyword(s):  
Diet Formulation

scholarly journals PSVI-24 Immunological Effects of a Purified 1,3/1,6 β-glucan Supplementation in Retorted Canine Diets

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 313-313
Author(s):  
Zac Traughber ◽  
Fei He ◽  
Maria R C de Godoy
Keyword(s):  
Mononuclear Cells ◽  
Control Diet ◽  
Fecal Microbiota ◽  
Significant Treatment ◽  
Peripheral Blood Mononuclear ◽  
Immune Parameters ◽  
Randomized Design ◽  
Diet Formulation ◽  
Respective Treatment ◽  
Branched Chain

Abstract Yeast cell wall products are common functional ingredients capable of “priming” the immune system, specifically in reference to vaccine efficacy. Twenty-four adult, female Beagles were used in a completely randomized design. Three retorted diets were used: control diet (CON), CON plus β-glucan top-dressed daily upon time of feeding (C+B), and CON plus retorted β-glucan included in diet formulation (BG). Following a 7 d adaptation to CON, dogs were fed their respective treatment diets for 42 d and were challenged with an oral Bordetella bronchiseptica vaccine on d 14 with blood collections on d 0, 21, 28, and 42. The objectives of the present study were 1) to evaluate the effects of dietary inclusion of a yeast β-1,3/1,6 glucan (150 ppm) on apparent total tract digestibility (ATTD) of macronutrients, fecal microbiota, and peripheral blood mononuclear cells (PBMC; T-cells, B-cells, and monocytes) of adult dogs and, 2) to test the effects of retorting on the efficacy of these β-glucans. All diets were well-accepted by all dogs. ATTD of both dry matter and crude protein were greater (P < 0.05) for BG than CON and with greater (P < 0.05) energy digestibility for BG than both B+G and CON. Additionally, fecal short-chain and branched-chain fatty acids, ammonia, indole and phenol concentrations did not differ among treatments. No significant treatment by time interactions among treatment groups were observed for any analyzed PBMC. These data suggest that a 150 ppm inclusion of this yeast-derived β-glucan had no detrimental effects on ATTD, fecal characteristics and metabolites, nor any analyzed PBMC; however, due to the absence of differences in immune parameters among treatments, the effect of retorting on the efficacy of this product cannot confidently be assessed with these findings. Higher doses of yeast-derived β-glucan might be needed to elicit an immunological modulation in healthy adult dogs.

scholarly journals 13 Opportunities for using low-protein diets for weanling pigs to improve intestinal health

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 18-19
Author(s):  
Martin Nyachoti ◽  
Jinyoung Lee
Keyword(s):  
Dietary Protein ◽  
Pathogenic Bacteria ◽  
Intestinal Microbiome ◽  
Gut Health ◽  
Intestinal Health ◽  
Dietary Interventions ◽  
Weanling Pigs ◽  
Bacterial Fermentation ◽  
Area Of Interest ◽  
Diet Formulation

Abstract Dietary manipulation with respect to crude protein (CP) content has been suggested as part of the overall strategy for the nutritional management of weanling pigs to improve intestinal health. This has focused on the use of low CP diets that are appropriately fortified with crystalline amino acids (AA). Use of low CP diets minimizes the amount of undigested dietary protein entering the large intestine and being subjected to bacterial fermentation. This is important because protein fermentation leads to the production of toxic metabolites and encourages the proliferation of pathogenic bacteria, thus causing enteric problems such as post-weaning diarrhea. There have been considerable efforts to elucidate the mechanisms underlying the potential benefits of feeding low CP diets to piglets. In addition to impacting the intestinal microbiome and its associated activities, it is clear that feeding a low CP diets interferes with the attachment of enterotoxigenic E. coli to the intestinal mucosa, thus minimizing its ability to cause disease. Another area of interest has been how use low CP diets in combination with other dietary manipulations to further enhance intestinal health in piglets. In this regards, existing evidence suggests that a low CP diet may be used in combination with other dietary interventions, such as probiotics and dietary fiber, to further enhance gut health outcomes in piglets. Also, addressing the potential reduction in piglet performance when feeding low CP diets by looking more into diet formulation to avoid deficiencies of essential AA or even some of non-essential AA, is critical for successful use low CP diets. Based on the available information, a reduction of dietary protein by four percentage units coupled with appropriate AA supplementation can be a useful dietary strategy to improve intestinal health.

scholarly journals 282 Effect of formulating diets based on a ratio between STTD Ca and STTD P and the inclusion of phytase on the calcium and phosphorus balance of growing pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 103-104
Author(s):  
Vanessa Lagos ◽  
Mike R Bedford ◽  
Hans H Stein
Keyword(s):  
Block Design ◽  
Random Effect ◽  
Growing Pigs ◽  
Diet Formulation ◽  
Randomized Complete Block Design ◽  
Microbial Phytase ◽  
P Utilization ◽  
P Balance ◽  
Main Effects ◽  
P Absorption

Abstract An experiment was conducted to test the hypothesis that formulating diets for pigs based on a ratio between standardized total tract digestible (STTD) Ca and STTD P instead of total Ca and STTD P increases the efficiency of Ca and P utilization. Forty barrows (59.4 ± 3.8 kg) were individually housed in metabolism crates and allotted to 4 corn-soybean meal diets and 2 periods of 11 d in a randomized complete block design. Diets were formulated using a 2 × 2 factorial design with 2 Ca requirement estimates (total Ca or STTD Ca) and 2 inclusion levels of microbial phytase (0 or 500 units/kg). Phytase was assumed to release 0.11% STTD P and 0.16% total Ca. Diets were formulated based on requirements for total Ca and STTD P and the ratio between STTD Ca and STTD P was 1.25:1 in diets formulated based on STTD Ca. Fecal and urine samples were collected from feed provided from d 6 to 9. Data for Ca and P balance were analyzed using a model that included the main effects of diet formulation and phytase level, the interaction between main effects, and the random effect of period. Interactions (P < 0.05) between diet formulation and phytase level were observed for Ca intake, Ca in feces, Ca digestibility, Ca retained as a percentage of intake, P digestibility, P absorbed, and P in urine (Table 1). Despite being provided less (P < 0.05) Ca, pigs fed diets formulated based on STTD Ca did not absorb or retain less Ca than pigs fed total Ca diets, but they absorbed more (P < 0.05) P than pigs fed diets formulated based on total Ca. In conclusion, by formulating diets based on values for STTD Ca, P absorption was increased confirming detrimental effects of oversupplying Ca.

PSVII-3 Evaluation of dietary OmniGen AF supplementation and PG600 injection on reproductive characteristics of gilts

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 411-411
Author(s):  
Jun Chen ◽  
Jung Wook Lee ◽  
Jim J Monegue ◽  
Richard Niblett ◽  
Kim Williams ◽  
...  
Keyword(s):  
Animal Health ◽  
Basal Diet ◽  
Corpora Lutea ◽  
Crown Rump Length ◽  
Reproductive Characteristics ◽  
Ovary Weight ◽  
Diet Formulation ◽  
Two Factors ◽  
Diet Control ◽  
Dietary Treatments

Abstract A cooperative study evaluated effects of supplemental dietary OmniGen AF (OG; Phibro Animal Health Corporation) on reproductive characteristics of gilts. Crossbred gilts (n = 56; initial body weight = 152.3 ± 9.7 kg) were assigned to treatments in a 2 x 2 factorial design [diet (OG or control) and PG600 (Merck Animal Health, El Dora, KS) injection (Yes or No)]. Dietary treatments were: 1) corn-SBM basal diet (control) that met NRC (2012) requirement estimates, or 2) control plus 0.75% OG and were fed 35 to 40 d prior to breeding. A common diet formulation was used by both stations. Gilts were estrous-synchronized by 14-d feeding of Matrix (Merck Animal Health). PG600 injections occurred 24 hours after Matrix withdrawal. Gilts were weighed at breeding and preslaughter (at d 39 to 48 postbreeding) and were euthanized to evaluate reproductive characteristics. There were no OG x PG600 interactions on any response measures (P > 0.15). Mean responses did not differ (P > 0.20) when gilts were fed control or OG diets, respectively, for total uterus weight (6,065 vs. 5,883 g), uterine horn length (129.2 vs. 123.9 cm), ovary weight (9.66 vs. 9.94 g), total corpora lutea (CL) number (25.0 vs. 25.3), individual CL weight (0.41 vs. 0.43 g), total follicular fluid weight (3.17 vs. 3.03 g), individual placenta weight (86.3 vs. 86.4 g), total fetuses (14.2 vs. 13.0), and fetus crown-rump length (7.97 vs. 7.66 cm). Supplementation with OG reduced (P = 0.04) individual placenta fluid volume of gilts (150.6 vs. 115.4 mL). PG600 injection increased (P < 0.001) ovary weight (9.03 vs. 10.57 g), total CL (18.09 vs 32.27) and reduced CL weight (0.46 vs. 0.38 g). The results demonstrated classic PG600 responses on reproductive characteristics but no effects of 0.75% OG supplementation nor an interaction between the two factors.

scholarly journals PSX-35 Late-Breaking Abstract: Use of total tract NDF and starch digestibility in beef cow and replacement heifer diet formulation

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 354-354
Author(s):  
Garland Dahlke ◽  
Devin Jakub ◽  
John Goeser ◽  
Erika L Lundy
Keyword(s):  
T Test ◽  
Neutral Detergent Fiber ◽  
Starch Digestibility ◽  
Energy Estimates ◽  
Actual Performance ◽  
Average Bias ◽  
Beef Cow ◽  
Commercial Laboratory ◽  
Diet Formulation ◽  
The Difference

Abstract The objective of this trial was to evaluate the effectiveness of using the total tract neutral detergent fiber (TTNDFd) and starch digestibility methodology in the formulation of beef cow and replacement heifer rations. This methodology ultimately applies to the estimation of energy availability to the animal and accompanied performance as outlined by the NASEM 2016 Nutrient Requirements of Beef Cattle publication. Multiparous, Angus cows during the last two months of gestation and yearling replacement, Shorthorn heifers comprised the study. Cows received one of four, dry ingredient diets while heifers received a corn silage-based diet. Feed nutrient evaluation along with intake were documented and applied to the NASEM model for these cattle. Results were compared to actual performance. Acid detergent fiber (ADF) derived energy estimates which generally accompany commercial laboratory feed analysis reports were compared as well. A T-test between actual and projected growth was used to describe the difference. The T-test between the TTNDFd/Starch derived results did not show any statistical difference between the actual and projected results for heifers P(T< =t) 0.15 with an average ADG bias of -0.06 Kg. The cow results over the four diets P(T< =t) ranged from 0.41 to 0.004 with an average bias of 0.04 to 0.27 Kg overestimating ADG. The T-test between the ADF derived results showed a difference between the actual and estimated values for heifers P(T< =t) 0.0004 with an average ADG bias of 0.2 Kg. The cow results over the four diets likewise over estimated available energy substantially. Here the test ranged from P(T< =t) 0.03 to 0.0001 with an average bias of 0.35 to 0.7 Kg. It appears that TTNDFd methodology should be strongly considered in the evaluation of forages and in the development of ration formulation software for beef offered high levels of fiber in their ration.

scholarly journals 281 Young Scholar Presentation: Digestibility of calcium and phosphorus in feed ingredients fed to gestating sows and growing pigs

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 111-111
Author(s):  
Su A Lee ◽  
Hans H Stein
Keyword(s):  
Serum Biomarkers ◽  
Growing Pigs ◽  
Hormonal Control ◽  
Late Gestation ◽  
Young Scholar ◽  
Diet Formulation ◽  
Microbial Phytase ◽  
The Impact ◽  
Ca:P Ratio

Abstract In practical diet formulation, values for standardized total tract digestibility (STTD) of Ca and P obtained in growing pigs are also applied to sows. However, gestating sows have reduced digestibility and retention of Ca and P compared with growing pigs, and the impact of microbial phytase on the digestibility of P and Ca is much less in sows than in growing pigs. Applying STTD values for Ca and P obtained in growing pigs to diets for gestating sows, therefore, results in an overestimation of the absorbed Ca and P in sows (Table 1). Further research, however, indicated that the digestibility of Ca and P in late gestating sows is greater than in sows in early or mid-gestation and retention of Ca and P was greater in late-gestation compared with earlier gestation periods, which indicates that digestion and absorption of Ca and P may be under hormonal control in sows (Table 2). It was also demonstrated that a wide Ca:P ratio decreased P digestibility in both growing pigs and sows in late-gestation, which demonstrates the need for not overfeeding STTD Ca. In follow-up research, it was demonstrated that several serum biomarkers may be used to predict if a sow is in a positive or a negative Ca and P state, but more research is needed to quantify this effect and to determine if biomarkers can be used in Ca and P requirement experiments. In conclusion, gestating sows have much lower digestibility of Ca and P than growing pigs, which demonstrates that digestibility values obtained in growing pigs cannot be used to accurately formulate diets for gestating sows. Likewise, effects of microbial phytase on digestibility of Ca and P are much less predictable in gestating sows than in growing pigs and phytase effects in sows are much smaller than in growing pigs.

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