Actin filaments, stereocilia and hair cells of the bird cochlea. VI. How the number and arrangement of stereocilia are determined

Development ◽  
1992 ◽  
Vol 116 (1) ◽  
pp. 213-226 ◽  
Author(s):  
L.G. Tilney ◽  
D.A. Cotanche ◽  
M.S. Tilney
Keyword(s):  
Hair Cell ◽  
Surface Area ◽  
Hair Cells ◽  
Apical Cell ◽  
Apical Surface ◽  
Cell Cytoplasm ◽  
Cross Sectional ◽  
Cross Sectional Profile ◽  
Sectional Profile ◽  
Round Cross

Beginning in 8-day embryos, stereocilia sprout from the apical surface of hair cells apparently at random. As the embryo continues to develop, the number of stereocilia increases. By 10 1/2 days the number is approximately the same as that encountered extending from mature hair cells at the same relative positions in the adult cochlea. Surprisingly, over the next 2–3 days the number of stereocilia continues to increase so that hair cells in a 12-day embryo have 1 1/2 to 2 times as many stereocilia as in adult hair cells. In short, there is an overshoot in stereociliary number. During the same period in which stereocilia are formed (9-12 days) the apical surface of each hair cell is filled with closely packed stereocilia; thus the surface area is proportional to the number of stereocilia present per hair cell, as if these features were coupled. The staircase begins to form in a 10-day embryo, with what will be the tallest row beginning to elongate first and gradually row after row begins to elongate by incorporation of stereocilia at the foot of the staircase. Extracellular connections or tip linkages appear as the stereocilia become incorporated into the staircase. After a diminutive staircase has formed, eg. in a 12-day embryo, the remaining stereocilia located at the foot of the staircase begin to be reabsorbed, a process that occurs during the next few days. We conclude that the hair cell determines the number of stereocilia to form by filling up the available apical surface area with stereocilia and then, by cropping back those that are not stabilized by extracellular linkages, arrives at the appropriate number. Furthermore, the stereociliary pattern, which changes from having a round cross-sectional profile to a rectangular one, is generated by these same linkages which lock the stereocilia into a precise pattern. As this pattern is established, we envision that the stereocilia flow over the apical surface until frozen in place by the formation of the cuticular plate in the apical cell cytoplasm.

scholarly journals Actin filaments, stereocilia, and hair cells of the bird cochlea. I. Length, number, width, and distribution of stereocilia of each hair cell are related to the position of the hair cell on the cochlea.

1983 ◽  
Vol 96 (3) ◽  
pp. 807-821 ◽  
Author(s):  
L G Tilney ◽  
J C Saunders
Keyword(s):  
Hair Cell ◽  
Surface Area ◽  
Hair Cells ◽  
Actin Filaments ◽  
Hexagonal Lattice ◽  
Frequency Discrimination ◽  
Sensory Epithelium ◽  
Scanning Microscopy ◽  
Apical Surface ◽  
Length Width

Located on the sensory epithelium of the sickle-shaped cochlea of a 7- to 10-d-old chick are approximately 5,000 hair cells. When the apical surface of these cell is examined by scanning microscopy, we find that the length, number, width, and distribution of the stereocilia on each hair cell are predetermined. Thus, a hair cell located at the distal end of the cochlea has 50 stereocilia, the longest of which are 5.5 microns in length and 0.12 microns in width, while those at the proximal end number 300 and are maximally 1.5 microns in length and 0.2 micron in width. In fact, if we travel along the cochlea from its distal to proximal end, we see that the stereocilia on successive hair cells gradually increase in number and width, yet decrease in length. Also, if we look transversely across the cochlea where adjacent hair cells have the same length and number of stereocilia (they are the same distance from the distal end of the cochlea), we find that the stereocilia of successive hair cells become thinner and that the apical surface area of the hair cell proper, not including the stereocilia, decreases from a maximum of 80 microns2 to 15 microns2. Thus, if we are told the length of the longest stereocilium on a hair cell and the width of that stereocilium, we can pinpoint the position of that hair cell on the cochlea in two axes. Likewise, if we are told the number of stereocilia and the apical surface of a hair cell, we can pinpoint the location of that cell in two axes. The distribution of the stereocilia on the apical surface of the cell is also precisely determined. More specifically, the stereocilia are hexagonally packed and this hexagonal lattice is precisely positioned relative to the kinocilium. Because of the precision with which individual hair cells regulate the length, width, number, and distribution of their cell extensions, we have a magnificent object with which to ask questions about how actin filaments that are present within the cell are regulated. Equally interesting is that the gradient in stereociliary length, number, width, and distribution may play an important role in frequency discrimination in the cochlea. This conclusion is amplified by the information presented in the accompanying paper (Tilney, L.G., E.H. Egelman, D.J. DeRosier, and J.C. Saunders, 1983, J. Cell Biol., 96:822-834) on the packing of actin filaments in this stereocilia.

X-Ray Replication of Submicrometer Linewidth Patterns

1977 ◽  
Vol 35 ◽  
pp. 136-137
Author(s):  
Henry I. Smith ◽  
D.C. Flanders
Keyword(s):  
Electron Beam Lithography ◽  
Cross Sectional ◽  
X Ray ◽  
Electron Backscattering ◽  
Spatial Frequencies ◽  
Cross Sectional Profile ◽  
Carbon Foils ◽  
Sectional Profile ◽  
And Control ◽  
Soft Radiation

Scanning electron beam lithography has been used for a number of years to write submicrometer linewidth patterns in radiation sensitive films (resist films) on substrates. On semi-infinite substrates, electron backscattering severely limits the exposure latitude and control of cross-sectional profile for patterns having fundamental spatial frequencies below about 4000 Å(l),Recently, STEM'S have been used to write patterns with linewidths below 100 Å. To avoid the detrimental effects of electron backscattering however, the substrates had to be carbon foils about 100 Å thick (2,3). X-ray lithography using the very soft radiation in the range 10 - 50 Å avoids the problem of backscattering and thus permits one to replicate on semi-infinite substrates patterns with linewidths of the order of 1000 Å and less, and in addition provides means for controlling cross-sectional profiles. X-radiation in the range 4-10 Å on the other hand is appropriate for replicating patterns in the linewidth range above about 3000 Å, and thus is most appropriate for microelectronic applications (4 - 6).

scholarly journals Bonding widths of Deposited Polymer Strands in Additive Manufacturing

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 871
Author(s):  
Cheng Luo ◽  
Manjarik Mrinal ◽  
Xiang Wang ◽  
Ye Hong
Keyword(s):  
Polymer Melt ◽  
Acrylonitrile Butadiene Styrene ◽  
High Viscosity ◽  
Numerical Results ◽  
Fused Filament Fabrication ◽  
Cross Sectional ◽  
Nozzle Height ◽  
Special Cases ◽  
Cross Sectional Profile ◽  
Sectional Profile

In this study, we explore the deformation of a polymer extrudate upon the deposition on a build platform, to determine the bonding widths between stacked strands in fused-filament fabrication. The considered polymer melt has an extremely high viscosity, which dominates in its deformation. Mainly considering the viscous effect, we derive analytical expressions of the flat width, compressed depth, bonding width and cross-sectional profile of the filament in four special cases, which have different combinations of extrusion speed, print speed and nozzle height. We further validate the derived relations, using our experimental results on acrylonitrile butadiene styrene (ABS), as well as existing experimental and numerical results on ABS and polylactic acid (PLA). Compared with existing theoretical and numerical results, our derived analytic relations are simple, which need less calculations. They can be used to quickly predict the geometries of the deposited strands, including the bonding widths.

Measurement of cross-sectional profile for sub-15nm hp LS patterns in nanoimprint templates using small-angle x-ray scattering

2020 ◽  
Author(s):  
Kazuki Hagihara ◽  
Eiji Yamanaka ◽  
Yoshiyasu Ito ◽  
Kiyoshi Ogata ◽  
Kazuhiko Omote ◽  
...  
Keyword(s):  
Small Angle ◽  
Cross Sectional ◽  
X Ray ◽  
X Ray Scattering ◽  
Cross Sectional Profile ◽  
Sectional Profile ◽  
Ray Scattering

Torsional Stiffness of a Thin-Walled Beam Braced by Pinned or Rigidly Fixed Transverse Diaphragms

1973 ◽  
Vol 15 (5) ◽  
pp. 351-356
Author(s):  
T. Harrison ◽  
J. M. Siddall
Keyword(s):  
Experimental Evidence ◽  
Theoretical Work ◽  
Torsional Stiffness ◽  
Cross Sectional ◽  
Cross Sectional Profile ◽  
Thin Walled ◽  
Sectional Profile ◽  
Good Agreement

The torsional stiffness of a thin-walled beam of open cross-sectional profile braced by evenly spaced transverse diaphragms is studied. Diaphragms rigidly fixed or attached by frictionless pins are treated and it is seen that, in either case, the only effect is to modify the St Venant torsional constant for the thin-walled beam. The theoretical work is supported by experimental evidence from two braced perspex channels which simulate the two assumed methods of attaching the diaphragms. Good agreement is demonstrated.

scholarly journals Influence of Geometric Parameters on Aerodynamic and Acoustic Performances of Bladeless Fans

2019 ◽  
Author(s):  
Ang Li ◽  
Jun Chen ◽  
Yangfan Liu ◽  
Stuart Bolton ◽  
Patricia Davies
Keyword(s):  
Cross Section ◽  
Fluid Dynamic ◽  
Geometric Parameters ◽  
Design Stage ◽  
Air Velocity ◽  
Cross Sectional ◽  
Future Design ◽  
Cross Sectional Profile ◽  
The Cross ◽  
Sectional Profile

Abstract In recent years, the bladeless fan that does not have visible impellers have been widely applied in household appliances. Since the customers are particularly sensitive to noise and the strength of wind generated by the fan, the aerodynamic and acoustic performances of the fan need to be accurately characterized in the design stage. In this study, computational fluid dynamic (CFD) and computational aeroacoustics (CAA) are applied to investigate the performances of different designs of a bladeless fan model. The influence of four parameters, namely the airfoil selection for cross-section of the wind channel, the slit width, the height of cross-section and the location of the slit, is investigated. The results indicate the streamwise air velocity increases significantly by narrowing the outlet, but the noise level increases simultaneously. In addition, the generated noise increases while the height of fan cross-section increases, and a 4mm height of the cross section is optimal for aerodynamic performance. When the slit is closer to the location of maximum thickness, the performances of the bladeless fan increases. Moreover, the performance is not changed significantly by changing the cross-sectional profile. Finally, the optimal geometric parameters are identified to guide the future design of the bladeless fan.

Twisting and Bending of Thin-Walled Prismatic Beams of Open Cross-Sectional Profile

1970 ◽  
Vol 12 (2) ◽  
pp. 130-134 ◽  
Author(s):  
T. Harrison
Keyword(s):  
Cross Sections ◽  
Virtual Work ◽  
Cross Sectional ◽  
Transverse Bending ◽  
Prismatic Beam ◽  
Loading System ◽  
Cross Sectional Profile ◽  
Thin Walled ◽  
Sectional Profile ◽  
Principle Of Virtual Displacements

Previous studies of the behaviour, in generalized co-ordinates, of thin-walled, prismatic beams of open cross-sectional profile have included, explicitly, only the effects of distributed transverse forces, q x and q y, distributed longitudinal forces, q z, and distributed torsional couples, m z. Using the principle of virtual displacements, the work of previous investigators is extended to include, quite generally, the effects of the hitherto neglected distributed couples, m x and m y. The derivation of the differential equation relating to the twisting of an open-section prismatic beam is presented fully whilst those relating to transverse and axial displacements of cross-sections are merely stated. The kinematic and static boundary conditions for a cantilever are also established from the virtual work equations. These show that the free-end shear boundary condition associated with transverse bending which is usually adopted in engineering calculations is inadequate for such a generalized loading system.

scholarly journals CORRELATION BETWEEN FIBER LENGTH, ULTRASTRUCTURE, AND THE LENGTH-TENSION RELATIONSHIP OF MAMMALIAN SMOOTH MUSCLE

1972 ◽  
Vol 52 (1) ◽  
pp. 105-116 ◽  
Author(s):  
Peter H. Cooke ◽  
Fredric S. Fay
Keyword(s):  
Fiber Length ◽  
Ultrastructural Organization ◽  
Cross Sectional ◽  
Dense Bodies ◽  
Tentative Model ◽  
Functional Consequences ◽  
Cross Sectional Profile ◽  
Sectional Profile ◽  
Basic Features ◽  
Relationship Of

The length-tension relationship was determined for strips of guinea pig taenia coli and correlated with the length and ultrastructural organization of the component fibers. The mean fiber length in "stretched" strips (passive ≥ active tension) was 30% greater than that for fibers in "unstretched" strips (active >> passive tension). In stretched fibers the dense bodies and 100 A diameter myofilaments were consolidated into a mass near the center of fibers in cross-sectional profile. The thick myofilaments were segregated into the periphery of the fiber profiles. In unstretched fibers the dense bodies-100 A diameter filaments and the thick myofilaments were uniformly distributed throughout cross-sectional profiles. A tentative model is proposed to account for the change in fiber length and ultrastructural organization that accompanies stretch. The basic features of the model require the dense bodies to be linked together into a network by the 100 A diameter filaments. The functional consequences of stretching the fibers are discussed in relation to the model proposed for this network.

scholarly journals Disentangling the Main Components of Hydromorphological Modifications at Reach Scale in Rivers of Greece

Hydrology ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 22
Author(s):  
Konstantinos Stefanidis ◽  
Anna Latsiou ◽  
Theodora Kouvarda ◽  
Anastasia Lampou ◽  
Nektarios Kalaitzakis ◽  
...  
Keyword(s):  
Habitat Modification ◽  
Ecological Quality ◽  
Cross Sectional ◽  
Habitat Features ◽  
Wide Range ◽  
Cross Sectional Profile ◽  
Sectional Profile ◽  
Main Components ◽  
The Individual ◽  
River Habitat Survey

The Water Framework Directive (WFD) requires from member states to monitor hydromorphological features of rivers in order to assess their ecological quality. Thus, numerous hydromorphological assessment methods have been developed with most of them focusing on the dynamics of hydrology, geomorphology and riparian zone extent. Within the scope of this study, we assessed the hydromorphological features of 106 river reaches distributed among thirteen WFD River Basin Districts (RBDs) to identify the main drivers of hydromorphological perturbation at a national scale. The studied reaches reflect a wide range of natural variability as they include various types of watercourses extending from lowlands to mid-altitude and mountainous systems. We employed the River Habitat Survey (RHS), and we recorded hydromorphological features and modifications in both banks and the channel bed along 500 m for each reach. Then, the Habitat Modification Score (HMS) and the individual sub-scores that indicate the extent of specific modifications (e.g., bridges, fords, weirs, bank reprofiling, bank reinforcement, etc.) were calculated in order to a) assess the severity of the total artificial modification and b) to highlight the most common and severe causes of overall alteration. The results showed that alterations such as reprofiling and reinforcement of banks contributed the most to the total HMS followed by the presence of fords and bridges. Particularly, the bank alterations indicate a serious deterioration of the longitudinal profile of the reaches, while the occurrence of many fords and bridges is the main cause for perturbations that affect locally the stream cross-sectional profile. Overall, these results compile a first nationwide assessment of the hydromorphological status of Greek rivers in line with the WFD and set the basis for further research that will focus on the diversity of stream habitat features as a measure for the overall ecological quality.

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